• The KIPS Transactions:PartD
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• v.19D no.3
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• pp.257-262
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• 2012

The aim of this study is to present the importance of Risk Management and Risk Classification System in Supply Chain to cope with the rapidly changing distribution environment flexibly through the cooperation between a shipper and a distribution company. First of all, we considered existing studies related to the risks of Supply Chain Risk and analyzed 47 different risk factors by 18 kinds of risk causing factors. Second, we collected opinions of corporation specialist group based on the analyzed risk factors and then classified the risk factors into three categories and ten sub-categories. Third, we conducted a survey targeting shipping companies and distribution companies about classified risk and then verified the validity of Supply Chain Risk Classification using verification techniques such as Confirmatory Factor Analysis, Concentration Validity and Distinction Validity. Finally, we suggest some implications based on the verification results.

• Korean Journal of Construction Engineering and Management
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• v.5 no.6 s.22
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• pp.72-79
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• 2004

Critical Path-based project management has been applied to the construction projects with a goal of delivering projects within original costs and time estimates. These current project management methods, rarely make early finishes of construction Projects. In addition, current practices on time management seems not to take advantages of early finishes concepts due to student syndrome and Parkinson's Law, This research study applied the Theory of Constraints(n) in the estimation of construction project duration. While the TOC includes variety of management techniques, in this study, it refers to critical chain that has been used to develop the specific management technique in scheduling. The concept of critical chain is applied to this study to solve the problems associated with the current scheduling method. The efforts focus to solve the p개blems associated with current construction project scheduling methods by adopting both stochastic estimation technique and the concept of schedule buffer,

• Toxicological Research
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• v.17
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• pp.109-115
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• 2001

Abrus agglutinin was purified from the kernels of Abrus precatorius by Sepharose 4B affinity column chromatography followed by Sephadex G-100 gel filtration column chromatography. About 1.25 g of abrus agglutinin was obtained from 1 kg of the kernels. The LD$_{50}$ of abrus agglutinin is 5 mg/kg of body weight, which is less toxic than that of abrin, 20$\mu\textrm{g}$/kg body weight. The amino acid sequence of abrus agglutinin was determined by protein sequencing techniques and deduced from the nucleotide sequence of a cDNA clone encoding full length of abrus agglutinin. There are 258 residues, 2 residues and 267 residues in the A-chain, the linker peptide and the B-chain of abrus agglutinin, respectively. Abrus agglutinin had high homology to abrin-a (77.8%). The 13 amino acid residues involved in catalytic function, which are highly conserved among abrin and ricin, were also conserved within abrus agglutinin. The protein synthesis inhibitory activity of abrus agglutinin ($IC_{50}$/ = 3.5 nM) was weaker than that of abrin-a (0.05 nM). By molecular modeling followed by site-directed mutagenesis showed that Pro199 of abrus agglutinin A-chain located in amphipathic helix H and corresponding to Asn200 of abrin A-chain, can induce bending of helix H. This bending would presumably affect the binding of abrus agglutinin A-chain to its target sequence GpApGpAp, in the tetraloop structure of 285 r-RNA subunit and this could be one of major factors contributing to the relatively weak protein synthesis inhibitory activity and toxicity of abrus agglutinin.n.

• The Journal of Society for e-Business Studies
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• v.14 no.4
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• pp.267-286
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• 2009

Most companies in automobile part industry need a SCM (Supply Chain Management) system under the cooperation with suppliers for assembling a automobile. Automobile part industry has a industry feature of hierarchical structure like 1st, 2nd, 3rd vendors. Recently, new cases to implement RFID (Radio Frequency Identification) technology appear in various areas required for integrated information system. Especially, supply chain environment in automobile part industry turns toward ubiquitous computing environment. Most companies in automobile part industry are interested in implementing RFID-based supply chain management system, which makes them shorten the business process, share the transaction information, and enhance the collaborative relationship between trading partners. This paper intends to implement RFID technology in the physical distribution area of automobile part industry such as warehousing, delivering, inventory management. The purpose of this paper is to improve the collaborative relationship of supply chain network using RFID applied to u-SCM system in ubiquitous computing environment. The contribution of this paper is to suggest a new system approach to transform existing business process into new business process through designing and implementing a RFID based SCM system using UML techniques.

• Journal of Aerospace System Engineering
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• v.14 no.2
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• pp.1-11
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• 2020

Detecting obstacles and generating a suitable path to avoid obstacles in real time is a prime mission requirement for UAVs. In areas, close to buildings and people, detecting obstacles in the path and estimating its own position (egomotion) in GPS degraded/denied environments are usually addressed with vision-based Simultaneous Localization and Mapping (SLAM) techniques. This presents possibilities and challenges for the feasible path generation with constraints of vehicle dynamics in the configuration space. In this paper, a near real-time feasible path is shown to be generated in the ORB-SLAM framework using a chain-based path planning approach in a force field with dynamic constraints on path length and minimum turn radius. The chain-based path plan approach generates a set of nodes which moves in a force field that permits modifications of path rapidly in real time as the reward function changes. This is different from the usual approach of generating potentials in the entire search space around UAV, instead a set of connected waypoints in a simulated chain. The popular ORB-SLAM, suited for real time approach is used for building the map of the environment and UAV position and the UAV path is then generated continuously in the shortest time to navigate to the goal position. The principal contribution are (a) Chain-based path planning approach with built in obstacle avoidance in conjunction with ORB-SLAM for the first time, (b) Generation of path with minimum overheads and (c) Implementation in near real time.

• Proceedings of the Korean Vacuum Society Conference
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• 2016.02a
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• pp.375.1-375.1
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• 2016

Polymerase chain reaction (PCR) has revolutionized genetics and become one of the most popular techniques in modern biological and medical sciences. It can be used not only as an in vitro DNA amplification method but also used in many bioassay applications. The PCR can be used to exponentially produce a large number of DNA copies from a small quantity of DNA molecules in a few hours. However, as unwanted DNA fragments are also often manufactured, the amplification efficiency of PCR is decreased. To overcome this limitation, several nanomaterials have been employed to increase the specificity of the PCR reaction. Recently, graphene has attracted a great interest for its excellent electron transfer, thermal and biocompatibility. Especially, gold nanoparticle-coated graphene oxide (GO/AuNPs) led to enhance electron and thermal transfer rate and low-charge transfer resistance. Therefore, we report the development of a demonstration for the PCR efficiency using a large-scale production of the GO and combination of gold nanoparticles. Because a thermal conductivity is an important factor for improving the PCR efficiency in different DNA polymerases and different size samples. When PCR use GO/AuNPs, the result of transmission electron microscopy and real-time quantitative PCR (qPCR) showed an enhanced PCR efficiency. We have demonstrated that GO/AuNPs would be simply outperformed for enhancing the specificity and efficiency of DNA amplification procedure.

• Clinical and Experimental Pediatrics
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• v.59 no.1
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• pp.43-46
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• 2016

We present a case of tuberculosis-associated hemophagocytic lymphohistiocytosis in a 14-year-old girl. The patient presented with weight loss, malaise, fatigue, prolonged fever, and generalized lymphadenopathy. Laboratory investigation revealed pancytopenia (white blood cells, $2,020cells/{\mu}L$; hemoglobin, 10.2 g/dL; platelets, $52,000cells/{\mu}L$), hypertriglyceridemia (229 mg/dL), and hyperferritinemia (1,420 ng/mL). Bone marrow biopsy showed a hypocellular bone marrow with a large numbers of histiocytes and marked hemophagocytosis; based on these findings, she was diagnosed with hemophagocytic lymphohistiocytosis. Polymerase chain reaction (PCR) with both the bone marrow aspiration and sputum samples revealed the presence of Mycobacterium tuberculosis. Antitubercular therapy with immune modulation therapy including dexamethasone and intravenous immunoglobulin was initiated. The results of all laboratory tests including bone marrow biopsy and PCR with both the bone marrow aspiration and sputum samples were normalized after treatment. Thus, early bone marrow biopsy and the use of techniques such as PCR can avoid delays in diagnosis and improve the survival rates of patients with tuberculosis-associated hemophagocytic lymphohistiocytosis.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.18 no.5
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• pp.259-265
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• 2018

As the supply method of products has become diverse and complex, problems such as manipulation of origin and product trace have occurred in the existing supply chain system. In order to deal with these issues, various Blockchain based studies have been actively carried in the SW industry and academy. However, there is a lack of comprehensive survey research on the applied Blockchain techniques, so that it hardly understand the pros and cons of the technologies. In this study, we intensively investigated the diverse studies from the Industrial and academic area based on the several criteria such as the goals, use of key Blockchain technologies, and IoT technologies.

• Journal of Korean Society on Water Environment
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• v.30 no.6
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• pp.683-690
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• 2014

Nanomaterials are used in a range of fields, including industry, medicine, aerospace, and manufacturing, due to their unique and useful properties. In recent years, nanotechnology has developed rapidly, and the amount of nanomaterials used in various fields has increased consistently. As a result, nanomaterials are released into the aquatic and soil ecosystem, posing potential risks to organisms and environment. These materials can enter the cells and may cause serious damage to organisms. Furthermore, they can be transferred through trophic levels and food web, thereby leading to bioconcentration and biomagnification. In this study, we analyzed the trends in research on food chain transfer of nanomaterials and investigated the techniques used in the research. Although many studies have been underway, there is a need for further advanced studies on higher trophic levels and complex microcosm and mesocosm. Furthermore, study topics should be expanded to include various types of nanomaterials and varied species and trophic levels.

• Journal of Microbiology and Biotechnology
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• v.5 no.4
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• pp.181-187
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• 1995

The cells of Vibrio vulnificus can be induced to the viable but nonculturable (VBNC) state by natural environmental parameters. The V. vulnificus cells in the VBNC state can not be recovered by ordinary laboratory techniques. This nonculturability could often hamper development of effective processing strategies to minimize the number of V. vulnificus in seafoods. Even with V. vulnificus cells in a culturable state, the length of time required to identify the bacteria in contaminated food by phenotyphic characterization may prevent appropriate in-time responses by public health agencies to infections of the bacteria. In the present study, we used polymerase chain reaction (PCR) to develop a rapid and direct detection method for V. vulnificus in small octopus (Octopus variabilis) which is consumed as a raw food in Korea. The region targeted was a 704-base pair (bp) portion of the hemolysin gene, vvhA, of V. vulnificus. The primers designed for PCR amplification were specific for all V. vulnificus sp. tested. Several methods were examined to extract total DNA directly from V. vulnificus seeded into the octopus homogenate and the guanidine isothiocyanate (CITC) method appeared to be most effective. From the octopus homogenate seeded by V. vulnificus at an initial level of $10^2$ CFU/ml of the homogenate and then incubated for 12 h, the targeted sequence was successfully amplified by PCR and the 704-bp DNA fragment was observed by gel electrophoresis. The total completion of this assay requires less than one day.