• 생명과학회지
• /
• 제17권1호
• /
• pp.91-96
• /
• 2007

본 연구는 적포도주의 첨가가 돈육 patty의 냉장 중 품질에 미치는 영향을 검토하기 위하여 적포도주를 첨가하지 않은 돈육 patty(대조구), 적포도주를 1% 첨가한 돈육 patty(RW-1), 적포도주를 3% 첨가한 돈육 patty(RW-3) 및 적포도주를 5% 첨가한 돈육 patty(RW-5)등 네 종류의 돈육 patty를 제조하고, 냉장 중 보수력, 표면색깔, pH, TBARS값, VBN 함량 및 총균수를 측정하였다. 보수력은 냉장 중 현저하게 증가하였으나, 적포도주의 첨가에 의한 영향은 없었다(p<0.05). 돈육 patty의 $L^*,\;a^*$ 및 $b^*$값은 적포도주 첨가에 의하여 낮아졌으며, 냉장 중 감소하는 경향이었다(p<0.05). pH는 냉장 기간이 경과하면서 증가하는 경향이었으며, 적포도주를 3 및 5% 첨가한 돈육 patty가 대조구 및 적포도주 1% 첨가구보다 낮게 나타났다(p<0.05). TBARS값은 냉장 기간이 경과하면서 현저하게 증가하였으며, 적포도주 첨가량이 증가함에 따라 TBARS값은 감소하는 경향이었다(p<0.05). 돈육 patty의 VBN함량은 냉장기간의 경과와 함께 유의하게 증가하였으며, 총균수도 냉장기간에 의하여 증가하였다. 그러나 적포도주의 첨가가 VBN 함량과 총균수의 증가를 억제하는 경향이었다.

• 한국식품영양과학회지
• /
• 제37권1호
• /
• pp.83-89
• /
• 2008

건조된 마늘을 분쇄하고 비율을 달리하여 소금과 혼합하여 마늘 소금을 개발하였고, 배소 시 김에 도포하여 마늘맛 조미 김을 개발하여 1, 6, 12주 동안 저장하면서 품질변화 및 지방산 산화정도를 측정하였다. 저장기간 및 마늘 첨가량에 따른 일반성분의 차이는 나타나지 않았다. 마늘 맛김의 tocopherol 함량을 분석한 결과 저장기간에 따라 ${\alpha}$-tocopherol은 감소하였고 특히 마늘 33% 첨가 시 감소량이 가장 적었다. ${\gamma}$-Tocopherol 함량은 저장기간에 따라 증가하였다. 또한, 지방산 중 linoleic acid 함량이 가장 높게 측정되었으나 저장기간 증가에 따라 큰 차이는 나타나지 않았다. 저장기간에 따른 TBARS 함량 변화는 대조군이 크게 증가한 반면, 마늘 맛김에서는 산화 억제효과가 나타났다. 마늘 파우더 33% 첨가는 12주 후에도 조미 김의 TBARS 생성량을 강하게 억제한 것으로 나타났다. 관능평가 결과 33% 마늘 소금을 첨가한 김이 색과 향 면에서 대조구와 유사하게 나타났다. 따라서 마늘의 첨가비율을 달리하여 제조한 마늘 소금으로 조미 김을 제조한 결과, 소금 대비 33%로 제조한 김이 다른 첨가군에 비교해 성분 및 관능적으로 우수하게 측정되었다.

• 약학회지
• /
• 제45권5호
• /
• pp.494-499
• /
• 2001

Houttuynia cordata (Saururaceae) has pungent smell and taste. It has been regarded as detoxicant, antipyretic, antiinflammantory and diuretic agents. In order to evaluate antioxidative and antilipidperoxidative efficacies, its fractions ($H_2O$, 20% MeOH, 40% MeOH, 60% MeOH, 170% MeOH) were measured by DPPH method and TBARS assay on rat liver homogenate. It was revealed that 60% MeOH fractions had potential antioxidative activity and inhibited lipid peroxidation significantly: In active fractions, we isolated rutin, hyperin, quercitrin and quercetin.

• 약학회지
• /
• 제47권6호
• /
• pp.361-364
• /
• 2003

Acanthopanax species (Araliaceae) has been traditionally used as tonic, analgesic, stimulant of immune system, and replenishment of body function. The antioxidant activities of leaf and root bark of Acanthopanax species were determined by 1,1-diphenyl-2-picrylhydrazyl (DPPH) method and thiobarbituric acid reactive substances (TBARS) assay and relative electrophoretic mobility (REM) on human plasma low density lipoproteins (LDL). Acanthopanax divaricatus var. albeofructus and Acanthopanax for. nambunensis showed potent antioxidant activities.

• 약학회지
• /
• 제44권4호
• /
• pp.354-357
• /
• 2000

Two ellagitannins, pedunculagin and 2,3-(S)-Hexahydroxydiphenoyl (HHDP)-D -glucose and three condensed tannins, (+)-catechin, (-)-epicatechin and procyanidin B-4 which were isolated from Rubus coreanum were evaluated for their antioxidative effects with 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and lipid peroxidation generation system mediated by addition of $H_2O$$_2$to rat liver homogenate (TBARS). The ellagitannins, 2,3-(S)-HHDP-D-glucose and pedunculagin, showed more potent antioxidative activities by DPPH and TBARS than condensed tannins.

• Entomological Research
• /
• 제48권5호
• /
• pp.453-456
• /
• 2018

The lipids of Clanis bilineata larvae meat (CBLM) are susceptible to oxidation, and thus the commercial and consumption values of CBLM decrease during frozen storage. In the present study, peroxide values (PV), thiobarbituric acid-reactive substances (TBARS), free fatty acid (FFA) content, fatty acid composition, and likeness (palatability) score of CBLM were determined to investigate the effect of glutathione on the oxidation of CBLM lipids. Glutathione decreased the PV, TBARS, and FFA content, maintained fatty acid composition, and increased the likeness (palatability) score of the CBLM, indicating that glutathione can be used as a cryoprotectant to extend the shelf life of CBLM.

• 한국식품영양과학회지
• /
• 제34권1호
• /
• pp.36-41
• /
• 2005

본 연구는 고콜레스테롤을 급여한 흰쥐 에 키토산 올리고당의 첨가 수준을 달리한 식이가 흰쥐의 지방대사에 미치는 영향을 알아보고자 수행하였다. 체중이 174.7$\pm$6.3 g인 Sprague-Dawley종 수컷 흰쥐에게 고콜레스테롤혈증을 유발시키기 위하여 콜레스테롤을 식 이 무게의 0.5%를 급여하고 키토산 올리고당을 식이 무게의 1.0%와 2.0%로 하여 총 3군으로 나누어 4주간 사육하였다. 식이섭취량과 체중증가량은 식이에 의한 영향이 없었다. 혈청 중 총 콜레스테롤, 중성지방은 대조군에 비해 2.0% 키 토산 올리고당을 첨가한 군에서 유의적으로 그 농도가 크게 감소하였으며 HDL-콜레스테롤/총콜레스테롤 비는 키토산 올리고당 첨가에 의해 높아지는 경향을 보였다. 간 조직 중 TBARS 함량은 키토산 올리고당을 첨가하였을 경우 유의적으로 낮게 나타났으며 SOD와 catalase의 활성은 키토산 올리고당을 첨가한 군이 대조군에 비해 유의적으로 낮았다. 이상의 결과를 볼 때 키토산 올리고당은 체내 지방대사에 있어 총콜레스테롤과 LDL-콜레스테롤, 중성지방 및 TBARS 함량을 낮추고 HDL-콜레스테롤/총 콜레스테롤 비는 높이는 효과를 나타냈으며, 또한 생체내 고콜레스테롤에 의한 산화적 스트레스로부터 간을 보호하는 것을 알 수 있었다.

• Journal of Nutrition and Health
• /
• 제40권2호
• /
• pp.105-110
• /
• 2007

The study was designed to observe antioxidant activities of conjugated linoleic acid (CLA) by determining antioxidant enzyme protein levels [cytochrome P4502 El (CYP2E1), Copper, Zinc-superoxide dismutase (CuZn-SOD), glutathione peroxidase (CSH-Px), glutathione S-transferase (GST)] by Western blot analysis and the levels of ${\alpha}$-tocopherol and 2-thiobarbituric acid reactive substances (TBARS) in the liver of chronically ethanol-treated rats. Sixty Sprague Dawley male rats were divided into 3 groups (Control, EtOH, EtOH+CLA). All rats were fed Lieber-DeCarli liquid diet for 4 weeks by pair-feeding against the EtOH group. The liquid diet was supplemented with 1.77g CLA mixture per kg diet in the EtOH+CLA group. Isocaloric maltose dextrin was added in replace of 50g ethanol (36%kcal) for the Control group. Ethanol ingestion significantly increased the levels of CYP2E1 protein and TBARS, but significantly reduced CuZn-SOD protein level and increased GST protein level. There was no significant effect on the level of GSH-Px protein and ${\alpha}$-tocopherol in the liver by ethanol. CLA supplementation with ethanol significantly increased the levels of CuZn-SOD, GSH-Px and GST and also significantly attenuated TBARS level, whereas there was no significant effect on the levels of CYP2E1 protein and ${\alpha}$-tocopherol by CLA. Overall, the CLA supplemented to ethanol could significantly increase the levels of CuZn-SOD, GSH-Px and GST proteins and reduce the level of TBARS in the liver of chronically ethanol-treated rats.

• 동의생리병리학회지
• /
• 제16권4호
• /
• pp.684-692
• /
• 2002

Cordyceps militaris has been known as a Chinese traditional medicine for the treatment of tuberculosis, asthma, kidney disease, debility and fatigue etc. This study was attempted to investigate the therapeutic effect of C. militaris extract on the cytotoxic activity of HepG2, human hepatocellular carcinoma cells and the liver damage induced by carbon tetrachloride in SD rats. C. militaris extracts inhibited significantly the proliferation of HepG2 cells in vitro. Carbon tetrachloride(CCl₄) caused a significant an increase in liver weight, serum aspartate aminotransferase(AST) and alanine aminotransferase(ALT) activity, alkaline phosphatase(ALP), serum thiobarbituric acid reactive substances (TBARS), microsomal TBARS, and decrease in microsomal detoxification enzymes (cytochrome P-450, P-450 reductase, cytochrome b5, b5 reductase). TBARS and ALP in serum pretreated with C. militaris extracts (300mg/kg/day, 600mg/kg/day) was significantly reduced compared to control group(CCl₄). Cytochrome b5 and b5 reductase activities were significantly increased in CM300 (300 mg/kg/day) and CM600 group(600 mg/kg/day), and cytochrome P-450 reductase was significantly increased in CM300 group. Pretreatment (100, 300, and 600 mg/kg/day for 7 days) of C. militaris with CCl₄ was significantly inhibited the accumulation microsomal TBARS and the significantly increased in the cytochrome P-450 activity. These results suggested that C. militaris (300mg/kg/day for 7 days) has appreciable therapeutic effect on CCl₄ induced hepatotoxicity.

• Journal of Microbiology and Biotechnology
• /
• 제19권8호
• /
• pp.810-817
• /
• 2009

The effect of cryoprotectants (maltodextrin+glycerol) and cryoprotectants+antioxidant [ascorbic acid and/or butylated hydroxytoluene (BHT)] mixtures on the survival, electrolyte leakage, and lipid degradation of freeze-dried Weissella paramesenteroides LC11 during storage was investigated and compared with that of the control (cells without additives) over a 90-day storage period at 4 or $20^{\circ}C$ in glass tubes with water activity ($a_w$) of 0.23. The survival, electrolyte leakage, and lipid degradation were evaluated through colony counts, electrical conductivity, and thiobarbituric acid reactive substances (TBARS) content, respectively. The fatty acids composition was determined by gas chromatography, in both the total lipid extract and the polar lipid fraction, and compared with that of the control after the 90-day storage period. As the storage proceeded, increases in leakage value and TBARS content, as well as a decrease in viability, were observed. After 90 days of storage, the major fatty acids found in both the total lipid extract and the polar lipid fraction were palmitic (16:0), palmitoleic (16:1), stearic (18:0), oleic (18:1), linoleic (18:2), and linolenic (18:3) acids. The survival, leakage value, TBARS content and 18:2/16:0 or 18:3/16:0 ratio were the greatest for the protected strain held at $4^{\circ}C$. Cells with the cryoprotectants+BHT mixture showed the highest percentage of survival and 18:2/16:0 or 18:3/16:0 ratio in both lipid extracts, as well as the lowest leakage value and TBARS content after the 90-day storage period. Drying cells with the cryoprotectants+BHT mixture considerably slowed down polar lipid degradation and loss of membrane integrity, resulting in improved viability during storage.