Browse > Article

Effects of Cordyceps militaris on $CCl_4$ - Induced Liver Damage and Cancer Cell (HepG2 Cell) Growth  

Kim San (Department of Opthamology Otoiaryngology and Dermatology, Oriental Medical Collage, Wonkwang University)
Hwang Choong yeon (Department of Opthamology Otoiaryngology and Dermatology, Oriental Medical Collage, Wonkwang University)
Kim Nam kwen (Department of Opthamology Otoiaryngology and Dermatology, Oriental Medical Collage, Wonkwang University)
Park Min cheul (Department of Opthamology Otoiaryngology and Dermatology, Oriental Medical Collage, Wonkwang University)
Kim Jin (Department of Opthamology Otoiaryngology and Dermatology, Oriental Medical Collage, Wonkwang University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.16, no.4, 2002 , pp. 684-692 More about this Journal
Abstract
Cordyceps militaris has been known as a Chinese traditional medicine for the treatment of tuberculosis, asthma, kidney disease, debility and fatigue etc. This study was attempted to investigate the therapeutic effect of C. militaris extract on the cytotoxic activity of HepG2, human hepatocellular carcinoma cells and the liver damage induced by carbon tetrachloride in SD rats. C. militaris extracts inhibited significantly the proliferation of HepG2 cells in vitro. Carbon tetrachloride(CCl₄) caused a significant an increase in liver weight, serum aspartate aminotransferase(AST) and alanine aminotransferase(ALT) activity, alkaline phosphatase(ALP), serum thiobarbituric acid reactive substances (TBARS), microsomal TBARS, and decrease in microsomal detoxification enzymes (cytochrome P-450, P-450 reductase, cytochrome b5, b5 reductase). TBARS and ALP in serum pretreated with C. militaris extracts (300mg/kg/day, 600mg/kg/day) was significantly reduced compared to control group(CCl₄). Cytochrome b5 and b5 reductase activities were significantly increased in CM300 (300 mg/kg/day) and CM600 group(600 mg/kg/day), and cytochrome P-450 reductase was significantly increased in CM300 group. Pretreatment (100, 300, and 600 mg/kg/day for 7 days) of C. militaris with CCl₄ was significantly inhibited the accumulation microsomal TBARS and the significantly increased in the cytochrome P-450 activity. These results suggested that C. militaris (300mg/kg/day for 7 days) has appreciable therapeutic effect on CCl₄ induced hepatotoxicity.
Keywords
Cordyceps militaris; CCl₄; HepG2 Cell;
Citations & Related Records
연도 인용수 순위
  • Reference
1 The biochemistry of animal cells, the effect of corticosteroids on leakage of enzyme from dispersed rat liver cell. /
[ Takeda, Y.;A. Ichihara;H. Tanioka;H. Inove. ] / J. Biol. Chem.
2 /
[ 김창민;신민교;안덕균;이경순 ] / 중약대사전
3 Protein measurement with the Folin-phenol reagent. /
[ Lowry, O.H.;H.J. Rosebrough;A.L. Farr;R.J. Randall. ] / J. Biol. Chem.
4 Classification of Cordyceps species based on protein banding pattern. /
[ Sung, J.M.;H.K.;Lee, Y.J.;Yoo, Y.S.;Choi, S.H.;Kim, Y.O.;Kim;G.H. Sung. ] / Kor. J. Mycol.
5 Carbon tetrachloride로 유발된 간 손상에 미치는 백화사설초와 동충하초의 효과. /
[ 김미려 ] / 동서의학
6 /
[ 진존인 ] / 도설 한방의약대사전 III(중국약학대전)
7 The effects of natural Cordyceps and the cultured Mycelia of Cordyceps sinensis on murine immune organs and functions of mononuclear phagocyte system. /
[ Chen, D.M. ] / Chin. J. Integr. Trad. West. Med.
8 Activation of murine peritoneal macrophage by the natural cordyceps and the cultured mycelia of Cordyceps sinensis. /
[ Zhang S.L. ] / Chin. J. Integr. Trad. West. Med.
9 Pharmacology of natural cordyceps and cultured mycelia of Cordyceps sinensis. 2. Effects on immunologic function. /
[ Tang R.G. ] / Chin. Trad. Herbal Drugs.
10 눈꽃 동충하초의 약물활성. /
[ 심진영;이연실;임순성;신국현;현진이;김성연;이은방 ] / 생약학회지
11 Rapid colorimetric assay for cellular growth and survival application to proliferation and cytotoxic assays. /
[ Mosmann, T. ] / J. Immunol. Methods
12 Serum lipoperoxide levels in patients suppering from liver disease. /
[ Suematsu, T.;T. Kamada;H. Abe;S. Kikuchi;K. Yagi. ] / Clin. Chim. Acta.   ScienceOn
13 High pressure liquid chromatographic separation of multiple forms of cytochrome P-450. /
[ Bansal, S.K.;J. Love;H.L. Gurtoo. ] / Biochem. Biophys. Res. Commun.   ScienceOn
14 The carbon monoxide-binding pigment of liver microsomes. I. Evidence for its hemoprotein nature. /
[ Omura, T.;R. Sato. ] / J. Biol. Chem.
15 Microsomal NADPH cytochrome c reductase of liver. /
[ William, C.H.Jr.;M. Kamin. ] / J. Biol. Chem.
16 Partial purification of NADH cytochrome b5 reductase from rabbit liver microsomes with detergents and its properties. /
[ Mihara, K.;R. Sato, ] / J. Biochem(Tokyo)
17 The genus Cordyceps and its allies. Sci /
[ . Kobayasi, Y. ] / Sci. Rept. Tokyo Bunrika daikaku Sect B
18 The inhibition of purine biosynthesis de-novo in Bacillus subtilis by cordycepin. /
[ Rottman, F.;A. Guatino. ] / Biochem. Biophys. Acta
19 Effects of Cordyceps sinensis on natural killer activity and colony formation of B16 melanoma. /
[ Xu, R.H.;X.E. Peng;G.Z. Chen;G.L. Chen ] / Chin. Med. J.
20 Oral toxicity of carbon tetrachloride: Acute, subacute, and subchronic studies in rats. /
[ Brucker, J.V.;M.F. Mackenzie;S. Muralidhara. ] / Fund. Appl. Toxicol.   ScienceOn
21 Hepatotoxic interactions of ethanol with allyl alcohol or carbon tetrachloride in rats. /
[ Berman, E.;D.E. House;J.W. Allis;J.E. Simmons. ] / J. Toxicol. Environ. Health
22 Hepatoprotective effect of Swertia chirata on rat. /
[ Mukherjee, S.;A. Sur ;B.R Maiti. ] / Indian J. Exp. Biol.
23 Inhibitory effect of eugenol on non-enzymatic lipid peroxidation in rat liver mitochondria. /
[ Nagababu, E.;N. Lakshmaiah. ] / Biochem. Pharmacol.   ScienceOn
24 ffect of Teucrium stocksianum on paracetamol-induced hepatotoxicity in mice. /
[ Rasheed, R.A.;B.H. Ali;A.K. Bashir. ] / Gen. Pharmacol.
25 /
[ Black, S.D.;M.J. Coon.;P. Ortize de Montellano(Eds.) ] / Cytochrome
26 Cytochrome P450: Multiplicity of isoforms, substrates, and catalytic and regulation mechenisms. /
[ Portor, T.D.;M.J Coon. ] / J. Biol. Chem.
27 Effect of eugenol on drug- metabolizing enzymes of carbon tetrachloride-intoxicated rat liver. /
[ Kumaravelu, P.;D.P. Dakshinamoorthy;S. Subramaniam;H. Devaraj;N.S. Devaraj. ] / Biochem. Pharmacol.   ScienceOn
28 Chemoprotective effect of methanol extract of Hedera rhombea leaves on the reversal of cytochrome P-450 activities induced by carbon tetrachloride. /
[ Hong, Y-S.;H-L. Kim;Y-S. Pae;S-S. Park. ] / J. Appl. Pharmacol.
29 간암의 약물요법. /
[ 김병주 ] / 대한소화기병학회잡지
30 Therapeutic options for the treatment of colorectal cancer following 5-fluorouracil failure. /
[ Henderson, C.A. ] / Semin Oncol.
31 간암의 한의학적 치료에 대한 문헌적 고찰. /
[ 김동희;김성훈 ] / 대한동의병리학회지
32 수종 한약재가 간암세포(HepG2)와 마우스 복강 대식세포에 미치는 영향. /
[ 강성용;한종현;조남근 ] / 대한본초학회지
33 Bletilla striata as a vascular embolizing agent in interventional treatment of primary hepatic carcinoma. /
[ Zheng, C.;G. Feng;H. Liang. ] / Chin. Med. J.
34 North American entomogenous Cordyceps. /
[ Mains, E.B. ] / Mycologia
35 Seasonal changes in the infection of pupae of the beech caterpilla, Quadricalcarifera punctatella (Motsh)(Lep., Notodonitidae), by Cordyceps militaris Link (Clavicipitales, Clavicipitaceae) in the soil of the Japanese beech forest. /
[ Kamata, N.;H. Sato;M. Shimazu ] / J. Appl. Ent.   ScienceOn
36 Inhibitory effect of Cordyceps sinensis on spontaneous liver metastasis of Lewis lung carcinoma and B16 melanoma cells in syngeneic mice. /
[ Nakamura, K.;Y. Yamaguch;S. Kagota;Y.M. Kwon;K. Shinozuka;M. Kunitomo. ] / Jpn. J. Pharmacol.
37 CCl4 toxicity. III. Hepatostructural and enzyme change. /
[ Dinman, B.D.;E.A. Hamdin;C.F. Fox,;W.J. Frajola W.J. ] / Arch. Environ. Health
38 Hematologic and clinical chemistry effect of 2,3,7,8-tetrachlorodibenzo- p-dioxin in laboratory animals. /
[ Zinkle, J.G.;J.G. Vos;J.A. Moore;B.N. Gupta. ] / Environ. Health Perspectives
39 Growth inhibitors against tumor cells in Cordyceps sinensis other than cordycepin and polysaccharides. /
[ Kuo, Y.C.;C.Y. Lin;W.J. Tsai;C.L. Wu;C.F. Chen;M.S. Shiao. ] / Cancer Invest.   ScienceOn
40 Antioxidant activity of the extracts from fruiting bodies of cultured Cordyceps sinensis. /
[ Yamaguchi, Y.;S. Kagota;K. Nakamura;K. Shinozuka;M. Kunitomo. ] / Phytother. Res.   ScienceOn
41 Anti-oxidation activity of different types of natural Cordyceps sinensis and cultured Cordyceps mycelia. /
[ Li, S.P.;P. Li;T.T. Dong;K.W. Tsim. ] / Phytomedicine   ScienceOn
42 Antioxidative and antimutagenic effects of the ethanol extract from Cordyceps militaris. /
[ Kim, M-N.;S-W. Oh;D-S. Lee ;S-S. Ham. ] / Kor. J. Postharvest Sci. Technol.,
43 /
[ 이상인(외5인) ] / 한약임상응용
44 Free radical mechanisms in tissue injury. /
[ Slater, T.F. ] / Biochem. J.
45 The scientific rediscovery of an ancient Chinese herbal medicine: Cordyceps sinensis. Part II. /
[ Zhu, J.S.;G.M. Halpern;K. Jones. ] / Ibid.
46 Effects allopurinol, (+)-cyanidanol-3 and dihydroquinoline-type antioxidants on rat hepatic microsomal cytochrome P-450 and monooxygenases. /
[ Horvath, T.;E. Karge;T. Javor;W. Klinger. ] / Int. J. Clin. Pharmacol. Ther. Toxicol.
47 Pathological mechanisms in carbon tertrachloride hepatotoxicity. /
[ Brattin, W.J.;E.A. Glendo;R.O. Recknagel. ] / J. Free Rad. Biol. Med.   ScienceOn