• 한국해양학회지:바다
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• 제17권3호
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• pp.160-180
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• 2012

본 연구는 메타게놈 분석법을 기초로 낙동강 하류 담수 환경의 물금과 기수 환경의 을숙도대교 정점에서 채수된 환경 시료내의 진핵 플랑크톤 종다양성 및 군집 구조를 비교 분석하고자 하였다. 수환경 시료에서 추출된 DNA에 대한 environmental Polymerase Chain Reaction(PCR)을 수행하여 18S rDNA 클론라이브러리를 구축하였고, colony PCR, PCR-Restriction Fragment Length Polymorphism(RFLP), 염기서열 결정 및 유사도 분석을 통하여 종다양성을 분석하였다. 물금 및 을숙도대교 정점에서 338개의 클론들을 분석하였고(170 clones, 물금; 168 clones, 을숙도대교), 그 결과 총 74개의 phylotype을 발굴하였다(49개, 물금; 25개, 을숙도대교). 발굴된 phylotype에 대한 계통 분석 결과, Stramenopiles, Cryptophyta, Viridiplantae, Alveolata, Rhizaria, Metazoa 및 Fungi 등의 분류군에 속하는 다양한 생물종이 발굴되었으며, 국내 미기록종 및 신종 후보 가능 생물종과 속(genus)이상의 새로운 분류학적 처리가 필요한 생물종의 존재를 확인하였다. 특히 Stramenopiles의 Pirsonia 및 Alveolata의 Perkinsea에 속하는 phylotypes 등 국내 미기록 생물종을 포함한 숨은 종다양성(cryptic species diversity)의 발굴은 분자모니터링 기법이 낙동강 하구역 수생태계 변화 모니터링을 위한 새로운 유용한 생물학적 정보를 제공할 수 있음을 제시하고 있다.

• Journal of Microbiology and Biotechnology
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• 제20권12호
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• pp.1717-1723
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• 2010

The integrated fixed-film activated sludge (IFAS) system is a variation of the activated sludge wastewater treatment process, in which hybrid suspended and attached biomass is used to treat wastewater. Although the function and performance of the IFAS system are well studied, little is known about its microbial community structure. In this study, the composition and diversity of the bacterial community of suspended and attached biomass samples were investigated in a full-scale IFAS system using a high-throughput pyrosequencing technology. Distinct bacterial community compositions were examined for each sample and appeared to be important for its features different from conventional activated sludge processes. The abundant bacterial groups were Betaproteobacteria (59.3%), Gammaproteobacteria (8.1%), Bacteroidetes (5.2%), Alphaproteobacteria (3.9%), and Actinobacteria (3.2%) in the suspended sample, whereas Actinobacteria (14.6%), Firmicutes (13.6%), Bacteroidetes (11.6%), Betaproteobacteria (9.9%), Gammaproteobacteria (9.25%), and Alphaproteobacteria (7.4%) were major bacterial groups in the attached sample. Regarding the diversity, totals of 3,034 and 1,451 operational taxonomic units were identified at the 3% cutoff for the suspended and attached samples, respectively. Rank abundance and community analyses demonstrated that most of the diversity was originated from rare species in the samples. Taken together, the information obtained in this study will be a base for further studies relating to the microbial community structure and function of the IFAS system.

• 한국토양비료학회지
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• 제26권3호
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• pp.215-221
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• 1993

중국(中國)은 1991년에 새로운 토양계통분류법(土壤系統分類法)을 1차(次) 시안(試案)으로 확정 발표하였다. 본 체계는 미국의 Soil Taxonomy 체계와 비슷한 형태계량적규준(形態計量的規準)(Morpho-metric definition)으로 되어 있고 전산(電算)프로그램화(化)도 가능한 체계로 되어 있다. 분류체계는 목(目)(Order, 토망(土網)), 아목(亞目)(Suborder, 아망(亞網)), 군(群)(Group, 토류(土類)), 아군(亞群)(Subgroup, 아류(亞類)), 속(屬)(Family, 토속(土屬)), 통(統)(Series, 토종(土種))으로 되어 있고, 현재 13개 목망(目網), 33개 아목(亞目), 77개 군(群), 301개 아군(亞群)으로 분류되어 있다. 특징적표층(特徵的表層)은 유기표층(有機表層) 등(等) 8종, 진단표하층(診斷表下層)(차표층(次表層))은 표백층(漂白層) 등(等) 10종, 기타 특징토층(特徵土層)은 석회적표층(石灰的表層) 등(等) 12종, 그리고 암성(岩性) 등(等) 23종의 토양 생성적(生成的) 특징(特徵) 또는 현상(現像)을 분류기준으로 쓰고 있다. 분류상의 특징은 고위도(高緯度)나 고산지대(高山地帶)의 영구동결층(永久凍結層)을 강조하여 아목수준(亞目水準)에서 분류하였고 논토양이나 관개퇴적토(灌漑堆積土), 영양분(營養分)이 심층(深層)까지 집적(集積)된 채전(菜田) 등(等) 인위적(人爲的) 영향(影響)을 크게 받은 토양을 최고차(最高次) 분류단위(分類單位)인 목수준(目水準)에서 독립(獨立)시키고 있을 뿐 아니라 인위적인 교란(攪亂)을 받았으나 아직 단면(斷面)의 발달이 없는 토양을 초육토(初育土)의 아목수준(亞目水準)에서 구분하고 있다.

• 한국균학회지
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• 제3권2호
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• pp.13-18
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• 1975

1974년(年) 9월(月) 5일(日)부터 1975(年) 7월(月) 30일(日)까지 소백산(小白山) 및 안동지방(安東地方)에서 50여종(餘種)의 고등균류(高等菌類)를 채집(採集)하여 동정(同定)하였다. 그중(中) 한국(韓國) 미기록종(未記錄種)은 다음과 같다. Hygrophorus pantoleucus Hongo 흰갓주름버섯, Marasmius prasiosmus (Fr.) Fr. 흰낙엽버섯, Conocybe lactea (Lange) Metrod 노란종버섯, Pholiota spimosa(Fr.) sing. 노란갓비늘버섯, Crepidotus sulphurinus Imaz. et Toki 노란귀버섯, Pulveroboletus retipes (Berk. et Curt.) Sing. 밤색비단그물버섯, Calodon zonatus (Fr.) $Qu{\acute{e}}l$. 갈색깔대기버섯 Patella scutellata (St. Amans) Morgan 주홍접시버섯, Peziza badia Pers. ex Merat. 자주주발버섯.

• Mycobiology
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• 제47권4호
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• pp.378-390
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• 2019

The Tian Shan mountain system is one of the large mountain ranges located in Central Asia. This region is globally recognized as mountain ranges, offering inestimable wealth in fauna and flora with significant biodiversity values. We surveyed macrofungal diversity of Tian Shan in Kyrgyzstan from 2016 to 2018. A collection of macrofungi was made, and these were subjected to sequence comparisons and phylogenetic analysis to ensure the identity of the collected macrofungi. Of those collected, 95 out of 100 specimens were successfully sequenced and compared with those of other related species retrieved from GenBank. The sequenced specimens were classified into 2 phyla, 8 orders, 24 families, 47 genera, and 57 species, based on current taxonomic concepts (combining morphology and phylogeny). To the best of our knowledge, this study provides the first well-documented checklist and phylogenetic analysis of macrofungi recovered from the Tian Shan mountains in Kyrgyzstan.

• Journal of Dairy Science and Biotechnology
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• 제30권2호
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• pp.131-137
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• 2012

Rapid and reliable identification of microorganisms is a key for tracing the relationship between the target bacteria and related infectious diseases. Various identification methods such as classical phenotypic analysis, numerical taxonomic analysis, and DNA sequencing have been widely used to classify microorganisms in milk and dairy products. Matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) identifies targeted bacteria in milk and milk products. Several studies have demonstrated that MALDI-TOF MS identification is an efficient and inexpensive method for the rapid and routine identification of isolated bacteria. MALDI-TOF MS could provide accurate identification of bacteria in milk and milk products at the serotype or strain level and enable antibiotic resistance profiling within minutes.

• Animal cells and systems
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• 제4권2호
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• pp.109-115
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• 2000

To clarify taxonomic status of the two sibling species, Moroco oxycephalus and M. lagowskii reproductive isolation mechanisms were investigated at sympatric area located in Kansung-up, Kosung-gun, Kangwon-do, Korea. Genetic analysis was performed to reveal mating system and intensity of Hybridization between the two species. The frequencies of hybrids were increased since 1989, and then the observed hybrid frequencies ($H_O$) did not significantly differ from the expected hybrid ($H_E$) in 1998 and 1999. However, based on histological analysis of two parents and their hybrid s gonads, the hybridizations between M. oxycephalus and M. lagowskii produced mostly fertile females but sterile males in accordance with Haldane s rule. Although it was suspected that pre- and postmating isolation mechanisms were affected between the two species, M. oxycephalus and M. lagowskii seemed to be strongly isolated with microhabitat at sympatry until 1997. Since 1998, hybrid frequencies were increased by habitat disturbance. However, their hybrid frequencies would be reduced by postmating isolation mechanisms. Therefore, the two species are considered to be distinct species recently diverged.

• 천문학회보
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• 제38권1호
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• pp.62.2-62.2
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• 2013

It is considered that comets near the ecliptic plane have been injected into inner solar system from Kuiper-belt. Some of them are still active while others are dormant with no detectable tails and comae. These dormant comets have eccentric and/or inclined orbits, which are parameterized by Tisserand parameter TJ<3. In addition, dormant comets can be differentiated from asteroids based on the albedo, because they have low albedo (the geometrical albedos pv<0.1). The conditions of TJ<3 and pv<0.1 have been used as a criteria to discriminate dormant comets from asteroids. However, we must be more careful because there are 'contaminations' from the outer region, i.e. Jovian Trojans (5.05

• 한국한의학연구원논문집
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• 제8권1호
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• pp.55-63
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• 2002

The plant origines of Atractylodis Rhizoma Alba and Atractylodis Rhizoma are genus Atractylodes*Compositae). Their origines in pharmacopoeia are slightly different among Korea, Japan, North Korea and China. The species of Atractylodis Rhizoma Alba are Atrctylodes japonica $K_{OIDZ}$. A. ovata $T_{HUNB}$.. Atractylodis Rhizoma are A. lancea DC., A. chinensis DC. and A. koreana $K_{ITM}$. But the texonomic genealogy of Atractylodis Rhizoma Alba is different from that of Atractylodis Rhizoma. The herbal origines of Atractylodis Rhizoma Alba and Atractylodis Rhizoma were not different at the early days. Since Tao-Hong-Jing(陶弘景) distinguished between Atractylodis Rhizoma Alba and Atractylodis Rhizoma at the very beginning, theri medicinal usages are different, Atractylodis Rhizoma have been used to remove pathogenic dampness in the digestive organs and Atractylodis Rhizoma Alba have been used to invigorate the digestive system and replenish qi. In additions, the figures and historical records of herbal appearance are different between Atractylodis Rhizoma Alba and Atractylodis Rhizoma. The diagnostics of them are in accord with the texonomic genealogies.

• 한국균학회지
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• 제5권2호
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• pp.1-9
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• 1977

한국(韓國)에 자생(自生)하는 야생(野生)버섯의 분포상(分布狀)을 조사(調査)하기 위해, 수원(水原), 광릉(光陵), 대관령(大關嶺), 지리산(智異山) 및 한라산(漢拏山)에서 무당 버섯속(屬)에 대(對)하여 조사(調査)한 결과(結果), 한국미기록종(韓國未記錄種) 3종(種)이 분류(分類)되었으며 기록종(記錄種) 29종(種)을 포함(包含)하여 32종(種)을 4아속(亞屬)에 배치기재(配置記載)하였다. 또한 한국(韓國) 미기록(未記錄) 3종(種)과 4아속(亞屬)에 대(對)하여 우리나라 이름을 명명(命名)하였다.