• Tribology and Lubricants
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• 제26권1호
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• pp.21-30
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• 2010

In this research, it is investigated the collecting efficiency of jet oil to several types of piston oil cooling gallery by using recently developed PCJ (piston cooling jet) rig tester. So it will be selected for a better design of piston oil cooling gallery. The collecting efficiencies at each type of piston cooling galleries are measured under conditions of a few piston positions, and several oil jet pressures and oil viscosities. Furthermore, the type of jet cone will be compared for a few jet pressure conditions. The selected type of piston oil cooling gallery is planned to be applied to the target engine which is now developing to satisfy the EURO VI emission regulation.

• Journal of Communications and Networks
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• 제18권5호
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• pp.846-856
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• 2016

Wireless sensor networks (WSNs) are widely applied in monitoring and control of environment parameters. It is sometimes necessary to disseminate data through wireless links after they are deployed in order to adjust configuration parameters of sensors or distribute management commands and queries to sensors. Several approaches have been proposed recently for data dissemination in WSNs. However, none of these approaches achieves both high efficiency and low complexity simultaneously. To address this problem, cluster-tree based network architecture, which divides a WSN into hierarchies and clusters is proposed. Upon this architecture, data is delivered from base station to all sensors in clusters hierarchy by hierarchy. In each cluster, father broadcasts data to all his children with instantly decodable network coding (IDNC), and a novel scheme targeting to maximize total transmission gain (MTTG) is proposed. This scheme employs a new packet scheduling algorithm to select IDNC packets, which uses weight status feedback matrix (WSFM) directly. Analysis and simulation results indicate that the transmission efficiency approximate to the best existing approach maximum weight clique, but with much lower computational overhead. Hence, the energy efficiency achieves both in data transmission and processing.

• KSBB Journal
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• 제31권4호
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• pp.300-311
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• 2016

Recently gene delivery has been designed newly using bioactive biomaterial and applied in the various field by many researchers. In this study, we proposed a new gene delivery system which has the capability of targeting effect in the specific tissue and remarkably enhanced transfection efficiency. We investigated $^1H-NMR$ spectroscopy, particle size analyzer and gel retardation to confirm the correct preparation of gene delivery. Also, we identified the hemo-compatibility of gene delivery by hemolysis assay, non-cytotoxicity by MTT test and transfection efficiency. The uptake mechanism of the gene carrier was confirmed using inhibitor agent such as sodium azide, indomethacin, quercetin, colchicine, and chloropromazine. As a results, it was identified that gene carrier prepared by in this study entered in the cell by the microtubule-dependent, energy-dependent and clathrin-mediated endocytosis pathway.

• 유통과학연구
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• 제20권9호
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• pp.97-107
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• 2022

Purpose: Online-To-Offline Food Delivery Services (O2O-FDS) is a new element in hotel supply chain that digitalizes traditional food delivery practices using new technologies. Targeting hotel guests, the present research is to explore the role of trust in the relationship between food delivery app on loyalty, price efficiency, and convenience. This new perspective is an essential value addition in the field of hotel marketing in relation to O2O-FDS. Research design, data and methodology: The present research conducted the mediator variable regression analysis as a main statistical methodology to identify the connections between three main constructs using total 244 South Korean participants that were recruited from the professional research company in Korea. Results: The statistical findings based on three steps of mediator regression approach strongly indicate that the trust of food delivery app in the hotel sector plays a moderating role in the relationship between price efficiency and convenience of food delivery app and customer loyalty of food delivery app in the hotel business. Conclusions: This research concludes that hotel practitioners must strive to provide adequate information on food delivery applications in order to increase price efficiency and convenience relative to traditional food and beverage services, influencing consumer trust in food delivery technology.

• 한국동물생명공학회지
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• 제38권3호
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• pp.99-108
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• 2023

Background: Efficient gene editing technology is needed for successful knock-in. Homologous recombination (HR) is a major double-strand break repair pathway that can be utilized for accurately inserting foreign genes into the genome. HR occurs during the S/G2 phase, and the DNA mismatch repair (MMR) pathway is inextricably linked to HR to maintain HR fidelity. This study was conducted to investigate the effect of inhibiting MMR-related genes using CdCl₂, an MMR-related gene inhibitor, on HR efficiency in HC11 cells. Methods: The mRNA and protein expression levels of MMR-related genes (Msh2, Msh3, Msh6, Mlh1, Pms2), the HR-related gene Rad51, and the NHEJ-related gene DNA Ligase IV were assessed in HC11 cells treated with 10 μM of CdCl₂ for 48 hours. In addition, HC11 cells were transfected with a CRISPR/sgRNA expression vector and a knock-in vector targeting Exon3 of the mouse-beta casein locus, and treated with 10 μM cadmium for 48 hours. The knock-in efficiency was monitored through PCR. Results: The treatment of HC11 cells with a high-dose of CdCl₂ decreased the mRNA expression of the HR-related gene Rad51 in HC11 cells. In addition, the inhibition of MMR-related genes through CdCl₂ treatment did not lead to an increase in knock-in efficiency. Conclusions: The inhibition of MMR-related gene expression through high-dose CdCl₂ treatment reduces the expression of the HR-related gene Rad51, which is active during recombination. Therefore, it was determined that CdCl₂ is an inappropriate compound for improving HR efficiency.

• 한국물환경학회지
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• 제39권6호
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• pp.465-474
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• 2023

The chemical composition and molecular weight characteristics of dissolved organic matter (DOM) exert a profound influence on the efficiency of organic matter removal in water treatment systems, acting as efficiency predictive indicators. This research evaluated the primary chemical and molecular weight properties of DOM derived from diverse sources, including rivers, lakes, and biomasses, and assessed their relationship with the efficiency of coagulation/flocculation treatments. Dissolved organic carbon (DOC) removal efficiency through coagulation/flocculation exhibited significant correlations with DOM's hydrophobic distribution, the ratio of humic-like to protein-like fluorescence, and the molecular weight associated with humic substances (HS). These findings suggest that the DOC removal rate in coagulation/flocculation processes is enhanced by a higher presence of HS in DOM, an increased influence of externally sourced DOM, and more presence of high molecular weight compounds. The results of this study further posit that the efficacy of water treatment processes can be more accurately predicted when considering multiple DOM characteristics rather than relying on a singular trait. Based on major results from this study, a predictive model for DOC removal efficiency by coagulation/flocculation was formulated as: 24.3 - 7.83 × (fluorescence index) + 0.089 × (hydrophilic distribution) + 0.102 × (HS molecular weight). This proposed model, coupled with supplementary monitoring of influent organic matter, has the potential to enhance the design and predictive accuracy for coagulation/flocculation treatments targeting DOC removal in future applications.

• 대한의용생체공학회:의공학회지
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• 제38권1호
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• pp.43-48
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• 2017

우리가 예상했던 DPNs의 지름은 약 500 nm였으며 이는 SEM과 AFM 영상, Size Distribution을 통해 기대했던 것과 유사한 크기를 가진다는 것을 확인하였다. 또한, Zeta potential은 약 $-17.8{\pm}4.4mV$으로 측정되었다. Zeta potential이 +30 mV이상이면 강한 양성을 띤다고 한다. 나노 입자의 Zeta potential이 강한 양성이면 nonspecific cellular interaction이 높아지지만 간에 의해 쉽게 제거되며, hemolytic activity가 높아지기 때문에 약물 전달을 하기에 적합하지 않은 것으로 알려져 있다. 또한 강한 음성이어도 간에 의해 제거될 확률이 높아진다. 하지만 나노 입자의 Zeta potential이 중성이거나 약한 전하를 띠면 혈액에서 제거가 잘 되지 않아 혈액에 오랫동안 남을 수 있어 약물전달에 유리하고, 약 -15 mV의 전하를 띤 입자는 tumor site에 high accumulation됨이 알려져 있다[14]. DPNs의 경우 $-17.8{\pm}4.4mV$이므로 인체에 적용하기에 적합한 것으로 판단된다. DPNs의 Encapsulation Efficiency는 약 $43.8{\pm}6.6%$로 Nano-precipitation과 같은 Bottom-up 방식보다 낮은 수치를 나타내었지만, 독성이 강한 Salinomycin을 사용함으로써 이를 해결할 수 있을 것으로 생각되며 적은 양의 약물만으로 항암효과를 나타낼 수 있을 것으로 기대된다. 암세포와 함께 배양했을 때 형광 현미경으로 확인해본 결과 암세포 주변에 나노 입자가 이동한 것으로 보아 Targeting ligand나 Peptide, Aptamer를 이용하면 더욱 정확한 암세포 표적화를 이룰 수 있을 것으로 예상된다[15]. DPNs의 Drug Carrier로서의 평가는 Loading Amount와 Drug Releasing Profile을 통해 추가로 검증을 할 예정이며, Cell viability를 실행하여 DPNs의 In vitro 항암 효과를 확인하고 In vivo 실험을 진행할 예정이다.

• 대한방사성의약품학회지
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• 제8권2호
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• pp.53-61
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• 2022

This study aimed to increase the targeting ability against PSMA in cell therapy using metabolic glycoengineering and biorthogonal chemistry and to visualize cell trafficking using PET imaging. Cellular membranes of THP-1 cells were decorated with azide(-N₃) using Ac₄ManNAz by metabolic glycoengineering. Engineered THP-1 cells were conjugated with DBCO-bearing fluorophore (ADIBO-Cy5.5) for 1 h at different concentrations and analyzed by confocal fluorescence microscopy and flow cytometry. For PSAM ligand conjugation to THP-1 cells, Ac₄ManNAz treated THP-1 cells were incubated with DBCO-PSMA ligand (ADIBO-GUL) at a final concentration with 100 µM for 1 h. To evaluate the effect on cell recognition, PSMA ligand conjugated THP-1 cells(as effectors) were co-cultured with PSMA positive 22RV1 (as target cells) at 3 : 1 a effector-to-target cell (E/T) ratio. The interaction between THP-1 and 22RV1 was monitored by confocal fluorescence microscopy. For preparing the radiolabeled THP-1, the cells were treated at the activity of ~ 740 kBq of [⁸⁹Zr]Zr(oxinate)₄/5 × 10⁶ cells. Radiolabeled cells were analyzed for determination of cell-associated radioactivity by gamma counting and viability using MTS assay. In the cytotoxicity assay, THP-1 cells did not have any cytotoxicity even when the Ac₄ManNAz concentration was 100 µM. In confocal microscopy and flow cytometry, THP-1 cells were efficiently labeled ADIBO-Cy5.5 in a dose-dependent manner, and the dose of 100 µM was the optimal concentration for the following experiments. The clusters of PSMA ligand-conjugated THP-1 cells and 22RV1 cells were identified, indicating cell-cell recognition over the cell surface between two types of cells. Cell radiolabeling efficiency was 54.5 ± 17.8%. THP-1 labeled with 0.09 ± 0.03 Bq/cell showed no significant cytotoxicity compared to unlabeled THP-1 up to 7 days. We successfully demonstrated that Ac₄ManNAz treated cells were efficiently conjugated with ADIBO-GUL for preparing the PSMA-targeting cells, and [⁸⁹Zr]Zr(oxinate)₄ could be used to label cells without toxicity. It suggested that PSMA-ligand conjugated cell therapy could be improved cell targeting and be monitored by PET imaging.

• 산학경영연구
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• 제23권2호
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• pp.53-75
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• 2010

강원도는 기업지원사업인 기술지원사업과 마케팅지원사업의 성과를 개별적으로 평가하여 왔으며, 기업을 대상으로 한 평가는 만족도 조사에 국한되어 왔다. 따라서 본 연구는 효율성 평가에서 그 유용성이 입증된 계량모형인 자료포락분석 (DEA : Data Envelopment Analysis)을 이용하여 기업지원사업에 참여하는 기업들의 효율성을 평가하는 모형을 개발하고 이 모형으로 기업의 효율성을 평가하였다. 기업지원사업의 투입요소와 산출요소를 기업지원사업의 특성에 맞는 변수로 모형을 개발하였으며, DEA 모형를 적용하여 기업에 대한 각각의 상대적 효율성 측정과 비효율적인 기업이 개선해야 할 부분을 계량적으로 제시하였다. 특히, 강원도내 기업의 기업지원 효율성을 처음으로 평가하였다는데 그 의의가 있다.

• 한국물환경학회지
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• 제36권5호
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• pp.405-422
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• 2020

Land use change by urbanization has significantly affected the hydrological process including the runoff characteristics. Due to this situation, it has been becoming more complicated to manage non-point source pollutions caused by rainfall. In order to effectively control non-point sources, it is necessary to identify the reduction efficiency of the various management method based on land use characteristics. Thus, the purpose of this study is to analyze the reduction efficiency of non-point source pollution management practices targeting three different watersheds with the different land use characteristics using the Soil and Water Assessment Tool (SWAT). To do this, the vulnerable subwatersheds to non-point source pollution occurrence within each watershed were selected based on the streamflow and water quality simulation results. Then, considering the land use, low impact development (LID) or best management practices (BMPs) were applied to the selected subwatersheds and the efficiency of each management was analyzed. As a result of analysis of the non-point source pollution reduction efficiency, when LID was applied to urban areas, the average reduction efficiencies of SS, NO₃-N, and TP were 5.92%, 4.62%, and 10.35%, respectively. When BMPs were applied to rural areas, the average reduction efficiencies of SS, TN and TP were 35.45%, 4.37%, and 10.16%, respectively. The results of this study can be used as a reference for determining appropriate management methods for non-point source pollution in urban, rural, and complex watersheds.