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http://dx.doi.org/10.7841/ksbbj.2016.31.4.300

Characteristic as a Gene Delivery System of Water Soluble Chitosan Conjugated with Cationic Peptide  

Kim, Young-Min (Department of Polymer Science and Engineering, College of Engineering, Sunchon National University)
Kim, Ji-Ho (Department of Polymer Science and Engineering, College of Engineering, Sunchon National University)
Park, Seong-Cheol (Department of Polymer Science and Engineering, College of Engineering, Sunchon National University)
Park, Yung-Hoon (Department of Polymer Science and Engineering, College of Engineering, Sunchon National University)
Jang, Mi-Kyeong (Department of Polymer Science and Engineering, College of Engineering, Sunchon National University)
Publication Information
KSBB Journal / v.31, no.4, 2016 , pp. 300-311 More about this Journal
Abstract
Recently gene delivery has been designed newly using bioactive biomaterial and applied in the various field by many researchers. In this study, we proposed a new gene delivery system which has the capability of targeting effect in the specific tissue and remarkably enhanced transfection efficiency. We investigated $^1H-NMR$ spectroscopy, particle size analyzer and gel retardation to confirm the correct preparation of gene delivery. Also, we identified the hemo-compatibility of gene delivery by hemolysis assay, non-cytotoxicity by MTT test and transfection efficiency. The uptake mechanism of the gene carrier was confirmed using inhibitor agent such as sodium azide, indomethacin, quercetin, colchicine, and chloropromazine. As a results, it was identified that gene carrier prepared by in this study entered in the cell by the microtubule-dependent, energy-dependent and clathrin-mediated endocytosis pathway.
Keywords
Gene carrier; Non-viral vector; Chitosan; transfection efficiency; Peptide;
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1 Wang, X., Z. Tai, J. Tian, W. Zhang, C. Yao, L. Zhang, Y. Gao, Q. Zhu, J. Gao, and S. Gao (2015) Reducible chimeric polypeptide consisting of octa-D-arginine and tetra-L-histidine peptides as an efficient gene delivery vector. Int. J. Nanomed. 10: 4669-4690.
2 Brus, C., H. Petersen, A. Aigner, F. Czubayko, and T. Kissel (2004) Physicochemical and biological characterization of polyethylenimine-graft-poly(ethylene glycol) block copolymers as a delivery system for oligonucleotides and ribozymes. Bioconjug. Chem. 15: 677-684.   DOI
3 Kamimura, K., T. Suda, G. Zhang, and D. Liu (2011) Advances in gene delivery systems. Pharmaceut. Med. 25: 293-306.
4 Chang, C. W., D. Choi, W. J. Kim, J. W. Yockman, L. V. Christensen, Y. H. Kim, and S. W. Kim (2007) Non-ionic amphiphilic biodegradable PEG-PLGA-PEG copolymer enhances gene delivery efficiency in rat skeletal muscle. J. Control. Release 118: 245-253.   DOI
5 Guo, X. and L. Huang (2012) Recent advances in nonviral vectors for gene delivery. Acc. Chem. Res. 45: 971-979.   DOI
6 Kim, W. J. and S. W. Kim (2009) Efficient siRNA delivery with non-viral polymeric vehicles. Pharm. Res. 26: 657-666.   DOI
7 Lee, M. and S. W. Kim (2005) Polyethylene glycol-conjugated copolymers for plasmid DNA delivery. Pharm. Res. 22: 1-10.   DOI
8 Choi, J. S., K. Nam, J. Y. Park, J. B. Kim, J. K. Lee, and J. S. Park (2004) Enhanced transfection efficiency of PAMAM dendrimer by surface modification with L-arginine. J. Control Release 99: 445-456.   DOI
9 Nam, H. Y., K. Nam, H. J. Hahn, B. H. Kim, H. J. Lim, H. J. Kim, J. S. Choi, and J. S. Park (2009) Biodegradable PAMAM ester for enhanced transfection efficiency with low cytotoxicity. Biomaterials 30: 665-673.   DOI
10 Tanaka, K., T. Kanazawa, T. Ogawa, Y. Takashima, T. Fukuda, and H. Okada (2010) Disulfide crosslinked stearoyl carrier peptides containing arginine and histidine enhance siRNA uptake and gene silencing. Int. J. Pharm. 398: 219-224.   DOI
11 Balicki, D., R. A. Reisfeld, U. Pertl, E. Beutler, and H. N. Lode (2000) Histone H2A-mediated transient cytokine gene delivery induces efficient antitumor responses in murine neuroblastoma. Proc. Natl. Acad. Sci. USA 97: 11500-11504.   DOI
12 Kaouass, M., R. Beaulieu, and D. Balicki (2006) Histonefection: Novel and potent non-viral gene delivery. J. Control Release 113: 245-254.   DOI
13 Kim, J. B., J. S. Choi, K. Nam, M. Lee, J. S. Park, and J. K. Lee (2006) Enhanced transfection of primary cortical cultures using arginine-grafted PAMAM dendrimer, PAMAM-Arg. J. Control Release 114: 110-117.   DOI
14 Wadhwa, M. S., W. T. Collard, R. C. Adami, D. L. McKenzie, and K. G. Rice (1997) Peptide-mediated gene delivery: Influence of peptide structure on gene expression. Bioconjug. Chem. 8: 81-88.   DOI
15 Gardlik, R., R. Palffy, J. Hodosy, J. Lukacs, J. Turna, and P. Celec (2005) Vectors and delivery systems in gene therapy. Med. Sci. Monit. 11: RA110-RA121.
16 Futaki, S., T. Suzuki, W. Ohashi, T. Yagami, S. Tanaka, K. Ueda, and Y. Sugiura (2001) Arginine-rich peptides. An abundant source of membrane-permeable peptides having potential as carriers for intracellular protein delivery. J. Biol. Chem. 276: 5836-5840.   DOI
17 Schnitzer, J. E., P. Oh, E. Pinney, and J. Allard (1994) Filipin-sensitive caveolae-mediated transport in endothelium: Reduced transcytosis, scavenger endocytosis, and capillary permeability of select macromolecules. J. Cell. Biol. 127: 1217-1232.   DOI
18 Kichler, A., A. J. Mason, and B. Bechinger (2006) Cationic amphipathic histidine-rich peptides for gene delivery. Biochim. Biophys. Acta 1758: 301-307.   DOI
19 Hyvonen, Z., A. Plotniece, I. Reine, B. Chekavichus, G. Duburs, and A. Urtti (2000) Novel cationic amphiphilic 1,4-dihydropyridine derivatives for DNA delivery. Biochim. Biophys. Acta 1509: 451-466.   DOI
20 Rejman, J., V. Oberle, I. S. Zuhorn, and D. Hoekstra (2004) Sizedependent internalization of particles via the pathways of clathrinand caveolae-mediated endocytosis. Biochem. J. 377: 159-169.   DOI
21 Wang, L. H., K. G. Rothberg, and R. G. Anderson (1993) Mis-assembly of clathrin lattices on endosomes reveals a regulatory switch for coated pit formation. J. Cell. Biol. 123: 1107-1117.   DOI
22 Dangoria, N. S., W. C. Breau, H. A. Anderson, D. M. Cishek, and L. C. Norkin (1996) Extracellular simian virus 40 induces an ERK/MAP kinase-independent signalling pathway that activates primary response genes and promotes virus entry. J. Gen. Virol. 77: 2173-2182.   DOI
23 Ou, M., R. Xu, S. H. Kim, D. A. Bull, and S. W. Kim (2009) A family of bioreducible poly(disulfide amine)s for gene delivery. Biomaterials 30: 5804-5814.   DOI
24 Templeton, N. S., D. D. Lasic, P. M. Frederik, H. H. Strey, D. D. Roberts, and G. N. Pavlakis (1997) Improved DNA: Liposome complexes for increased systemic delivery and gene expression. Nat. Biotechnol. 15: 647-652.   DOI
25 Deshpande, D., P. Blezinger, R. Pillai, J. Duguid, B. Freimark, and A. Rolland (1998) Target specific optimization of cationic lipidbased systems for pulmonary gene therapy. Pharm. Res. 15: 1340-1347.   DOI
26 Christensen, L. V., C. W. Chang, W. J. Kim, S. W. Kim, Z. Zhong, C. Lin, J. F. Engbersen, and J. Feijen (2006) Reducible poly(amido ethylenimine)s designed for triggered intracellular gene delivery. Bioconjug. Chem. 17: 1233-1240.   DOI
27 Kim, T. I., M. Ou, M. Lee, and S. W. Kim (2009) Arginine-grafted bioreducible poly(disulfide amine) for gene delivery systems. Biomaterials 30: 658-664.   DOI
28 Ou, M., X. L. Wang, R. Xu, C. W. Chang, D. A. Bull, and S. W. Kim (2008) Novel biodegradable poly(disulfide amine)s for gene delivery with high efficiency and low cytotoxicity. Bioconjug. Chem. 19: 626-633.   DOI
29 Fischer, D., T. Bieber, Y. Li, H. P. Elsasser, and T. Kissel (1999) A novel non-viral vector for DNA delivery based on low molecular weight, branched polyethylenimine: effect of molecular weight on transfection efficiency and cytotoxicity. Pharm. Res. 16: 1273-1279.   DOI
30 Kim, S. H., J. H. Jeong, T. I. Kim, S. W. Kim, and D. A. Bull (2009) VEGF siRNA delivery system using arginine-grafted bioreducible poly(disulfide amine). Mol. Pharm. 6: 718-726.   DOI
31 Kay, M. A. (2011) State-of-the-art gene-based therapies: The road ahead. Nat. Rev. Genet. 12: 316-328.   DOI
32 Niidome, T., M. Urakawa, H. Sato, Y. Takahara, T. Anai, T. Hatakayama, A. Wada, T. Hirayama, and H. Aoyagi (2000) Gene transfer into hepatoma cells mediated by galactose-modified alpha-helical peptides. Biomaterials 21: 1811-1819.   DOI
33 Nam, J. P. and J. W. Nah (2016) Target gene delivery from targeting ligand conjugated chitosan-PEI copolymer for cancer therapy. Carbohydr. Polym. 135: 153-161.   DOI