• The Korean Journal of Physiology and Pharmacology
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• v.26 no.3
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• pp.183-193
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• 2022

MicroRNAs (miRNAs) regulate gene expression and are biomarkers for coronary atherosclerosis (AS). A novel miRNA-mRNA regulation network of coronary AS still needs to be disclosed. The aim of this study was to analyze potential mRNAs in coronary AS patients and the role of their upstream miR-491-5p in vascular smooth muscle cells (VSMCs). We first confirmed top ten mRNAs according to the analysis from Gene Expression Omnibus database (GSE132651) and examined the expression levels of them in the plaques and serum from AS patients. Five mRNAs (UBE2G2, SLC16A3, POLR2C, PNO1, and AMDHD2) presented significantly abnormal expression in both plaques and serum from AS patients, compared with that in the control groups. Subsequently, they were predicted to be targeted by 11 miRNAs by bioinformatics analysis. Among all the potential upstream miRNAs, only miR-491-5p was abnormally expressed in the plaques and serum from AS patients. Notably, miR-491-5p overexpression inhibited viability and migration, and significantly increased the expression of contractile markers (α-SMA, calponin, SM22α, and smoothelin) in VSMCs. While silencing miR-491-5p promoted viability and migration, and significantly suppressed the expression of α-SMA, calponin, SM22α, and smoothelin. Overall, miR-491-5p targeted UBE2G2, SLC16A3, and PNO1 and regulated the dysfunctions in VSMCs.

• Journal of Microbiology and Biotechnology
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• v.32 no.12
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• pp.1605-1614
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• 2022

The strains associated with foodborne Salmonella enterica Thompson outbreaks in Korea have not been identified. Therefore, we characterized S. Thompson strains isolated from chocolate cakes linked to foodborne outbreaks in Korea. A total of 56 strains were isolated from preserved cake products, products in the supply chain distribution, the manufacturer's apparatus, and egg white liquid products used for cream preparation. Subsequently, serological typing, pathogenic gene-targeted polymerase chain reaction (PCR), pulsed-field gel electrophoresis (PFGE), and whole-genome multi-locus sequence typing (wgMLST) were performed to characterize these isolates. The antigen formula of all isolates was 7:k:1,5, namely Salmonella enterica subsp. enterica Serovar Thompson. All 56 isolates harbored invA, his, hin, and stn, and were negative for sefA and spvC based on gene-targeted PCR analyses. Based on PFGE results, these isolates were classified into one group based on the same SP6X01.011 pattern with 100% similarity. We selected 19 strains based on the region and sample type, which were subjected to wgMLST. Although the examined strains showed 100% similarity, they were classified into seven clusters based on allelic differences. According to our findings, the cause of these outbreaks was chocolate cake manufactured with egg white liquid contaminated with the same Salmonella Thompson. Additionally, comparative analysis of wgMLST on domestic isolates of S. Thompson from the three outbreaks showed genetic similarities of over 99.6%. Based on the results, the PFGE and wgMLST combination can provide highly resolved phylogeny and reliable evidence during Salmonella outbreak investigations.

• Journal of Photoscience
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• v.3 no.1
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• pp.23-28
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• 1996

To investigate the structure and function of photosystem I, cartridge mutagenesis technique was used to inactivate the psaB gene of photosystem I. From the screen, many strains which have potential defects in photosystem I were generated. Biochemical analysis revealed that B2, one of the mutant, had a reduced amount of chlorophyll. Electron transfer activitx from photosystem II to photosystem I as oxygen uptake was the rate of 64 % of wild type. Also B2 showed a decreased photosystem I activity when measured by 77 K fluorescence emission spectrum. Particularly, immunodetection analysis showed that the B2 had reduced amount of PsaA/PsaB, but a normal range of PsaC and PsaD. Here we present a photoheterotrophic mutant for psaB gene as a unique model strain for future study of structural/functional relationship and biogenesis of photosystem I.

• Proceedings of the KSME Conference
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• 2001.06d
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• pp.807-812
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• 2001

This paper represents the wall thinning analysis results for secondary side piping of two types of domestic nuclear power plants based on the DB establishment and F AC analysis study for NPP secondary system piping. CHECWORKS code utilized in this study has been applied world widely to wall thinning analyses for secondary side piping and its reliability has also been proved. The predicted wear rates for several piping systems of a pressurized water reactor NPP are compared with those of a pressurized heavy water reactor NPP and with the measured wear rates. On the basis of comparison results of the predicted and measured wear rates, the analysis results can be effectively applied to the development of a standard thinned pipe management program targeted all domestic nuclear power plants.

• Knowledge Management Research
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• v.16 no.2
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• pp.29-45
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• 2015

The number of studies on social media has increased rapidly worldwide. There has been, however, little effort to assess the achievements and limitations of social media studies up to present. The purpose of this study is to provide a systematical viewpoint on social media research published by scholarly business-related journals. Our analysis focus on research topic, functionality, targeted media types, methodology, and characteristics. User research receives the most attention, followed by effect research in terms of developmental models of media research agenda. The major methodological trend is online survey. Facebook and twitter are the two most popular mediums studied in literature, and social media is mainly characterized as information sharing as well as relationship building. Two recommendations are suggested in ways to strengthen social media research: more various topics and application areas, and the rigor and diversity of research methods.

• KIEAE Journal
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• v.16 no.1
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• pp.95-101
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• 2016

This study aims to analyze the satisfaction targeted community space for public rental housing tenants a real community space. and community space is analyzed only through the factor analysis of the effects of satisfaction, multiple regression analysis etc. As a result, the space community and public rental housing will only affect the satisfaction, factors affecting such analysis was 'common space', 'exercise place', 'resting place'. Thus, a variety of political and institutional efforts and practical research for interactive spatial planning public rental housing estates satisfaction for the improvement and community activation moving away from passive concept of the one-way ever to supply that community space in the past, provider centers and considering the tenant characteristics is required.

• The Journal of the Acoustical Society of Korea
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• v.25 no.3E
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• pp.89-94
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• 2006

For audio indexing and targeted search of specific audio or corresponding visual contents, the MPEG-7 standard has adopted a sound classification framework, in which dimension-reduced Audio Spectrum Projection (ASP) features are used to train continuous hidden Markov models (HMMs) for classification of various sounds. The MPEG-7 employs Principal Component Analysis (PCA) or Independent Component Analysis (ICA) for the dimensional reduction. Other well-established techniques include Non-negative Matrix Factorization (NMF), Linear Discriminant Analysis (LDA) and Discrete Cosine Transformation (DCT). In this paper we compare the performance of different dimensional reduction methods with Gaussian mixture models (GMMs) and HMMs in the classifying video sound clips.

• Convergence Security Journal
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• v.12 no.4
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• pp.97-108
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• 2012

In this study, we analysis the distributing malware using email on the korean defense service and defense industry as the social engineering attack. E-mail attack distributes the document files with the malware. Using the malware, attacker get the Information of the targeted people and devices. we proposed expected new types of attacks by analysis and transformation. And, expect the new email attack agendas which will be tried.

• Proceedings of the Korean Association of Geographic Inforamtion Studies Conference
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• 2003.11a
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• pp.9-14
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• 2003

The major reason that spatial data warehousing has attracted a great deal of attention in business GIS in recent years is due to the wide availability of huge amounts of spatial data and the imminent need for turning such data into useful geographic information. Therefore, this research has been focused on designing and implementing the pilot tested system for spatial decision making. The purpose of the system is to predict targeted marketing area by discriminating the customers by using both transaction quantity and the number of customer using credit card in department store. Focused on the analysis methodology, the case study is aiming to use GIS and clustering for knowledge discovery. The system is a key section of the research of multi-dimensional and spatio-temporal analysis in the internet environment.

• Journal of the Korean Home Economics Association
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• v.49 no.8
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• pp.113-124
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• 2011

The aim of this study is to conduct an evaluation review of the contents of the career education unit in textbooks on technology and home economics for senior high school freshmen. This study adopted the "Objective & System of Career Education" established by the Korea Research Institute for Vocational Education & Training as the criterion for evaluating the content of career education, and targeted the unit, "Design of Vocation & Careers", which was included in six different textbooks on technology and home economics for senior high school freshmen involving five different publishers. The unit of "Design of Vocation & Careers" was mostly written in three subunits. An analysis of the textbooks by different, publisher showed that every aspect of the "Objective & System of Career Education" was appropriately covered in the unit. This was achieved by means of the elements of contents and illustrations.