• BMB Reports
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• v.34 no.4
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• pp.365-370
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• 2001

We constructed a comparative expression system in order to produce recombinant human ferritin homo- and heteropolymers in Escherichia coli. Human ferritin H-(hfH) and L-chain (hfL) genes were expressed without amino acid changes under the control of a tac promoter. Ferritin heteropolymers of varying subunit composition were also produced by combining two different expression systems, a bicistronic expression system and a coplasmid expression system. As a result, recombinant H-chain ferritin and ferritin heteropolymers were catalytically active in forming iron core in vivo. In particular, the ferritin heteropolymer that is composed of 7% H-subunit and 93% L-subunit was capable of forming an iron core of the protein, while the L-chain ferritin homopolymer was inactive in vivo. This result indicates that the two H-subunits (i.e., 7% H-subunit content) are important to keep ferritin active in the cells. In addition, human ferritins were identified as the major iron binding proteins in the transformed cells. Also, the amount of iron bound to the recombinant ferritins was proportional to the H-subunit content in ferritin heteropolymers in vivo.

• Nutrition Research and Practice
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• v.8 no.4
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• pp.417-424
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• 2014

BACKGROUD/OBEJECTIVES: It is hypothesized that obese people with dyslipidemia is more likely to have increased oxidative stress and decreased antioxidant status, in comparison with the controls who were obese without dyslipidemia. Thus, the aims of the present study were to determine the dietary intakes, plasma adipokines, and antioxidative systems between obese with dyslipidemia and obese without dyslipidemia were investigated. SUBJECTS/METHODS: Female subjects who were between 20 and 55 years old, and whose BMI was 23 or greater were recruited. Subjects who met the criteria of $BMI{\geq}23$, total cholestero ${\geq}200mg/dL$, LDL cholesterol ${\geq}130mg/dL$, and $TG{\geq}110mg/dL$ were categorized Obese with dyslipidemia. Anthropometric measurements and blood biochemical tests were conducted. The diet survey was conducted by a trained dietitian using two days of 24 hour dietary recall. The lipid peroxidation, the plasma total antioxidant capacity (TAC), the activities of antioxidantive enzymes, and various antioxidantive vitamins levels were determined. RESULTS: Plasma adiponectin and leptin levels were also determined. There were no significant differences for age, Body Mass index (BMI), and body fat (%), waist-size between two groups. Obese with dyslipidemia had significantly high levels of total cholesterol, triglyceride, LDL-cholesterol, the ratio of total cholesterol/HDL-C, and the ratio of HDL-C/LDL-C, respectively. Blood alkaline phosphatase level was statistically different between the two groups (P < 0.05). No statistical significance in dietary intake between two groups was shown. In case of obese with dyslipidemia group, the levels of GSH-Px (P < 0.05) and catalase (P < 0.05) as well as adjusted blood retinol (P < 0.05) and tocopherol level (P < 0.05) were significantly low. However, the plasma concentration of leptin was significantly high (P < 0.05). CONCLUSIONS: Obesity with dyslipidemia was shown to have high arthtrogenic index, depleted antioxidant status, and higher blood leptin levels which suggest higher risks of oxidative stress and cardiovascular diseases.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.21 no.10
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• pp.1879-1885
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• 2017

This paper discusses the trajectory tracking system of unmanned ground vehicles based on predictive control. Because the unmanned ground vehicles can not satisfactorily complete the path tracking task, highly efficient and stable trajectory control system is necessary for unmanned ground vehicle to be realized intelligent and practical. According to the characteristics of unmanned vehicle, this paper built the kinematics tracking models firstly. Then studied algorithm solution with the tools of the optimal stability analysis method and proposed a tracking control method based on the model predictive control. The controller used a kinematics-based prediction model to calculate the predictive error. This controller helps the unmanned vehicle drive along the target trajectory quickly and accurately. The designed control strategy has the true robustness, simplicity as well as generality for kinematics model of the unmanned vehicle. Furthermore, the computer Simulink/Carsim results verified the validity of the proposed control method.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.37B no.10
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• pp.912-920
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• 2012

In this paper, we derived a closed-form equation of a Best Linear Unbiased Estimator (BLUE) estimator for the 3 dimensional estimation of the position of the emitter based on the Time Difference of Arrival (TDOA) technique. The BLUE derived for the case of estimating 3 dimensional position of the emitter with 4 base stations or sensors, and for this purpose, we used an approximated equation of the TDOA hyperbola equation obtained from the first order Taylor-series after setting the reference points of the position. The derived equation can be used for any kind of noises which are uncorrelated in each other in the TOA measurement noises and for a white Gaussian noise also.

• Korean Journal of Veterinary Service
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• v.38 no.4
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• pp.253-257
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• 2015

Bull-boxing in South Korea is a form of competitions in which bulls measure their strength with each other in the form of pushing by putting their heads together. Bull-boxing has been admitted after being designated as a play culture by the government because it does not cause great injuries to animals' bodies and has strong positive aspects such as contributing to livestock farmers' leisure activities, encouragement of animal husbandry, and communities' economic development. However, bull-boxing sometimes causes damage to the head and horns due to the heavy bodies. According to the results of examinations of these damage cases, damage that caused irregular shapes of the base of horns was identified in 31.5% of fighting bulls. The damage to horns is thought to have been caused by the great forces of fighting bulls repeatedly imposed to each other's horns during bull-boxing that caused minute fractures leading to damage to the blood vessels inside the horns resulting in the blocking of delivery of nutrients to the horns causing the deformation of the horns into abnormal shapes. Since bulls' bodies are injured during bull-boxing although the injuries are small as bull-boxing is mainly conducted by pushing, animal protection groups regard bull-boxing as cruelty to animals and request to stop bull-boxing. The present study aims to develop animal welfare type headgears that can protect bulls' horns and heads in order to protect fighting bulls during bull-boxing in terms of animal protection and welfare.

• Analytical Science and Technology
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• v.18 no.4
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• pp.329-337
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• 2005

TL dating using pre-dose effect is concerned with the sensitivity increase of $110^{\circ}C$ TL peak in quartz following the pre-exposure of irradiation (pre-dose) in antiquity and heating to thermal activation temperature. Using the multiple activation (MA) method for determining an equivalent dose, the activation temperature was selected to $500^{\circ}C$ and the region of interest was selected from $85^{\circ}C$ to $105^{\circ}C$ on quartz sample from archaeological remains, roof-tile kiln. which was excavated at Chudong-Ri cultural site. It was concluded that the resultant date was determined to the age of $1725{\pm}25years$ AD (l ${\sigma}$ SD), which had more improved precision considering with e radiocarbon dates and was in good agreement with the age derived by archaeological assessment. Also it was concluded that the pre-dose dating should contribute significantly to future archaeological dating work in the range of the last 2000 years.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.19 no.6
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• pp.547-556
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• 2007

The sediment transport by waves, wave-induced current and tidal current was calculated using the TRANSPOR2004, then the seasonal sediment budget was analyzed. Also, annual sediment budget was calculated, and sediment circulation patterns was deduced in the broad area including Haeundae beach. A sediment mainly inflows from the east coast of the beach and then moves to the eastward to the Dongback Is, where the 80% of inflow sediment transported to the eastward as a longshore sediment while 20% of them going out to the offshore at the center of the beach. Above results shows a good agreement with the sediment transport trend analysis results by the Gao model.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.19 no.6
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• pp.574-585
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• 2007

To analyze the incident wave characteristics around Haeundae beach the long-term deep water wave data computed by wave hindcast method were used and a continuous wave observation was carried out for 1 year at the 20 m of water depth in front of Haeundae beach. Wave observation data showd that the prevalent wave direction was SSW-S in spring and summer seasons while E-SE in autumn and winter. A numerical modeling shows that the waves from E-SE are refracted strongly due to the shoal developed at the south-east side of Haeundae beach. The simulation also shows inflowing nearshore current along the east coast of the beach develops strongly in autumn and winter. Radioactive isotope tracer experiment for 155 days indicated that the tracers moves to the on-shore direction in the 1st and 2nd tracking then dispersed to the E-W direction along the shore.

• The Korean Journal of Pain
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• v.24 no.4
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• pp.216-220
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• 2011

Background: Transforaminal epidural steroid injection is one of the effective treatments in managing radicular pain. There have been some prospective studies on the depth to the epidural space with the transforaminal approach. However, there have been no studies about the depth in Asians, especially Koreans. This study was carried out in order to evaluate the depth to the epidural space and the oblique angle and factors that influence the depth to the epidural space during lumbar transforaminal epidural injection. Methods: A total of 248 patients undergoing fluoroscopically guided transforaminal epidural steroid injections were evaluated. At the L3-4, L4-5, L5-S1, and S1 levels, we measured the oblique angle and depth to the epidural space. Results: Needle depth was positively associated with body mass index (correlation coefficient 0.52, P = 0.004). The median depths (in centimeters) to the epidural space were 6.13 cm, 6.42 cm, and 7.13 cm for 50-60 kg, 60-70 kg, and 70-80 kg groups, respectively, at L5-S1. Age and height were not significantly associated with the needle depth. Conclusions: There is a positive association between the BMI (and weight) and transforaminal epidural depth but not with age, sex, and height.

• Journal of Microbiology and Biotechnology
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• v.18 no.11
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• pp.1853-1857
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• 2008

Salmonella contamination in chicken meat was studied with 100 chicken meat samples purchased from 55 shops located in various regions. A total of 21 isolates of Salmonella enterica were isolated from 21 chicken meat samples from four shops located at open markets, whereas there were none from supermarkets with well-equipped cold systems. Among these, 18 isolates were identified as Salmonella enterica serotype Haardt (S. Haardt) and three isolates were S. enterica serotype Muenchen. When the minimal inhibitory concentrations of the S. Haardt isolates were assayed with the agar dilution method to determine susceptibility to ampicillin, chloramphenicol, sulfisoxazole, tetracycline, and nalidixic acid, all 18 isolates were resistant to tetracycline and nalidixic acid and nine of these were resistant to ampicillin. These isolates showed reduced susceptibility to eight fluoroquinolones including ciprofloxacin, enrofloxacin, levofloxacin, gatifloxacin, gemifloxacin, moxifloxacin, norfloxacin, and ofloxacin. When quinolone resistance determining regions of gyrA and gyrB were sequenced, every isolate had the same missense mutation Ser83$\rightarrow$Tyr (TCC$\rightarrow$+TAC) in gyrA, whereas no mutation was found in gyrB. Pulsed-field gel electrophoresis with XbaI revealed a close relationship among these isolates, suggesting a contamination of raw chicken meat with clonal spread of nalidixic acid-resistant and quinolone-reduced susceptibility S. Haardt in chickens. Results in this study show the importance of a well-equipped cold system and the prudent use of fluoroquinolone in chickens to prevent the occurrence of quinolone-resistant isolates.