• Title/Summary/Keyword: tBHP

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Antioxidant Activity of Extract from Walnut Uuglans sinensis Dode) and Its Protective Effect on Cell Injury and Lipid Peroxidation in Renal Cortical Slices (호두 추출물의 항산화 활성과 신피질에서 세포 손상과 지질과산화 방지효과)

  • Bae Kae Sun;Hwang Eul Chul;Kwon Chae Hwa;Kim Soon Hee;Choi Chun Whan
    • Journal of Life Science
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    • v.15 no.1 s.68
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    • pp.106-111
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    • 2005
  • To investigate the antioxidant activity of extract from the raw walnut, Juglans sinensis Dode, we prepared five fractions (methanol (MeOH), dichloromethane $(CH_2Cl_2)$, ethyl acetate (EtOAc), n-buthanol (n-BuOH) and dehydrogen monooxide $(H_2O)$ fractions) and examined. The effect of walnut extract on the oxidative stress was investigated in vitro. The DPPH (2,2-Di (4-tert-octylphenyl)-1-picrylhydrazyl) free radical scavenging activity of extract from raw walnut was shown in the following order: $EtOAc\;fraction layer. The result showed that the highest activity $(0.56{\mu}g/ml,\;IC_{50}.)$ was observed in EtOAc fraction, whereas n-BuOH fraction, MeOH fraction, $CH_2O_2$ fraction and $H_2O$ layer of $IC_{50}$ were $2.34{\mu}g//ml,\;3.88{\mu}g/ml,\;8.06{\mu}g/ml,\;and\;8.19{\mu}g/ml$, respectively. The radical scavenging activity assay of each fraction showed that the antioxidative activity was observed in the following order: EtOAc fraction $(74.27\pm1.56\%)>MeOH\;fraction\;(60.76\pm3.4\%)>n-BuOH\;fraction\;(59.32\pm0.88\%)>H_2O\;layer\;(41.69\pm2.06\%)$. These results revealed that all fractions, except for $CH_2Cl_2$ fraction, showed high antioxidative activity. Furthermore, the peroxynitrite $(ONOO^-)$ scavenging activity was assayed in each fraction. The result showed that the $ONOO^-$ scavenging activity of EtOAc fraction, MeOH fraction and n-BuOH fraction from raw walnut was $95.14\pm0.36\%,\; 90.02\pm1.19\%\;and\;89.41\pm0.81\%$, respectively. The tert-butylhydroperoxide (t-BHP) treatment in vitro increased lactate dehydrogenase release and lipid peroxidation in renal cortical slices. Such changes were completely prevented by addition of MeOH fraction, EtOAc fraction and n-BuOH fraction of walnut. These results indicate that the walnut extract exerts the benedicial effect against t-BHP-induced cell injury and its effect may be due to antioxidant action. In addition, it is suggested that walnut extract might be developed as the effective scavenger for the prevention of oxidative stress.

Antioxidant Effect of Salvia miltiorrhiza

  • Kang, Hye-Sook;Chung, Hae-Young;Jung, Jee-Hyung;Kang, Sam-Sik;Choi, Jae-Sue
    • Archives of Pharmacal Research
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    • v.20 no.5
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    • pp.496-500
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    • 1997
  • A strong antioxidant activity, which was measured by the radical scavenging effect on 1, 1-diphenyl-2-picrylhydrazyl(DPPH) radical, was detected in the methanol extract of Salvia miltiorrhiza Bunge (Labiatae). By activity-directed fractionation, compounds 1 and 2 were isolated as antioxidant principles of S. miltiorrhiza. Compounds 1 and 2 were identified as dimethyl lithospermate and 3-(3, 4-dihydroxyphenyl)lactamide, respectively, on the basis of spectral data. The radical scavenging effect of compounds 1 and 2 on DPPH radical exceeded that of L-ascorbic acid which is a well known antioxidant. These two compounds also showed prominent inhibitory activity against free radical generation in dichlorofluorescein (DCF) method and cytoprotective effect against t-BHP in cultured liver cell.

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The Characteristic of Tuna Longliners ( 1 ) - On the effective horse power and propulsion efficiency - (참치 연승어선의 특성 ( 1 ) - 유효마력과 추진효율 -)

  • 고장권
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.11 no.1
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    • pp.21-25
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    • 1975
  • Authors studied the propulsion efficiency of G. T. 280 to 300 class tuna longliners used in Korea. At first authors calculated the ratios and factors of the ship by use of principal dimensions. In cordance with this ratios and factors authors, examined the effective horse power, propulsion ficiency and brake horse power, and following results are obtained. (1) EHP is slightly increased according to the increasing of Froude number and speed. (2) Value of propulsion coefficient is obtained in the range of 51-54% at the cruising speed an its average is 52% in 6-13 knot of the speed. (3) EHP and BHP are increased according to the increasing of prismatic coefficient at the same speed.

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Experimental Determination of Friction Characteristics for Advanced High Strength Steel Sheets (초고강도강판 마찰특성의 실험적 규명)

  • Kim, N.J.;Keum, Y.T.
    • Transactions of Materials Processing
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    • v.22 no.4
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    • pp.223-228
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    • 2013
  • The friction coefficients of advanced high strength steel sheets were experimentally determined. In the friction test, the pulling and holding forces acting on the sheet for various friction conditions, such as lubricant viscosity, pulling speed, blank holding pressure, sheet surface roughness, and hardness of the sheet were measured and the friction coefficient was calculated based on Coulomb's friction law. While the friction coefficient, generally, decreases as the value of friction factor increases, the factor associated with the sheet surface roughness shows U shape behavior for the friction coefficient. Furthermore, the relationship between friction coefficient and the wear volume, which was computed for the roughness of both sheet surfaces and the friction area, is linearly proportional.

Comparison of Antioxidative Activity on Fish and Bovine Skin Gelatin Hydrolysates Produced in a Three-Step Membrane Enzyme Reactor (3단계 막효소반응기에서 연속적으로 생산된 어피 및 우피 젤라틴 가수분해물의 항산화활성 비교)

  • 김세권;박표잠;송병권;김종배
    • KSBB Journal
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    • v.15 no.6
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    • pp.635-643
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    • 2000
  • To compare the antioxidative activities of fish skin and bovine skin gelatin hydrolysate, gelatin hydrolysates from Alaska pollack and bovine skin were prepared by various enzymatic hydrolysis methods (1st step, Alcalase; 2nd step, pronase E; 3rd step, collagenase) using a continuous three-step membrane reactor. The molecular weight distributions of the 1st, 2nd and 3rd step hydrolysates were 7∼10 kDa, 2∼5 kDa and 0.7∼0.9 kDa, respectively. The antioxidative activity of fish skin gelatin hydrolysate was stronger than that of bovine skin gelatin hydrolysate, and in particular, both of 2nd step hydrolysates showed more antioxidative activity than hydrolysates of any other step. The optimum antioxidative activity concentration of the 2nd step hydeolysates of fish and boving skin were 1% (w/w) in a linoleic acid water-alcohol emulsion. In cultured cells exposed to t-butyl hydroperoxide (t-BHP), the 2nd step hydrolysate of fish skin gelatin delayed cell death most. These results suggest that the antioxidative activity of fish skin gelatin hydrolysate is higher than that of bovine skin gelatin hydrolysate because of their different amino acid contents.

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온라인 증권거래 서비스의 고객만족 요인에 대한 실증적 연구

  • Choe, Don-Hwang;Hong, Jae-Beom;Yang, Jeong-Sik
    • Proceedings of the Korea Association of Information Systems Conference
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    • 2005.05a
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    • pp.161-175
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    • 2005
  • 본 연구에서는 온라인 증권거래 서비스 품질과 고객만족 나아가 고객행동을 분석한 연구이다. 이를 위해 설문조사를 실시하였으며 조사원이 직접 증권사 객장을 방문하여 운라인 증권거래경험이 있는 고객을 대상으로 설문조사를 실시하였다. 이를 위해 40개 증권사, 420여개설문이 회수되었다. 분석결과, 첫째, 온라인 증권거래를 이용하는 고객층을 분석하면, 여성보다는 남성이 온라인 증권거래를 활발히 이용하고 있으며 연령별로는 40대, 50대, 30대 순으로 나타났으며 직업은 대부분 근로생활자가 많이 활용하고 있는 것으로 나타났으며 전체 50%이상이 매일 접속하고 거래하는 것으로 나타났으며 운영 규모는 1천만원이하가 다수로 나타났다. 둘째, 온라인 증권거래 서비스 품질요인으로 지각된 신뢰성, 지각된 편의성, 지각된 유형성, 지각된 응답성 순으로 고객만족에 영향을 미치는 것으로 나타났다. 지각된 신뢰성은 약속된 서비스를 정확하게 수행하는 능력에 대한 지각으로 나타내며, 지각된 편의성은 온라인증권거래를 이용하기에 얼마나 용이한 지에 대한 지각을 나타낸다. 지각된 유형성은 물적 시설, 장비, 사람, 의사소통 도구의 외형과 같은 부분에 대한 지각을 나타내는 것이다 지각된 응답성은고객을 돕고 즉각적인 서비스를 제공하려는 의지에 대한 지각을 나타낸다. 마지막으로 고객만족은 재이용이나 구전의도에 긍정적인 효과를 미치는 것으로 나타났다. 이러한 결과는 고객지향적인 온라인 증권거래 서비스 시스템 구축 및 운영이 이용확대에 중요한 요인임을 제시한다. 본 연구는 온라인 증권거래에 대한 고객만족의 탐색적인 연구로써 보다 심도 있는 연구를 위한 기초를 제공하였다는 점과 온라인 증권거래 성과측정요소로써 고객만족에 영향을 미치는 요인을 제시했다는 점에서 의미를 지닌다.에 의해 유도된 single-strand 절단을 억제하였다. 이상과 같이 간세포 일차배양에서 양파추출물은 t-BHP에 의해 유발된 간독성, 간세포 생존율 감소, 지질과산화를 농도 의존적으로 억제시켰고 또한 t-BHP에 의해 억제된 GSH-Px, GSH-Rd 및 catalase의 활성을 증가시켰다. 이와 같이 양파추출물의 간보호 및 항산화 효과는 항산화 효소, 특히 catalase의 활성 증가와 hydroxyl radical에 의해 유도된 산화억제 및 이에 따른 지질과산화 억제에 기인하는 것으로 사료된다.chno-economic paradigm)의 시각에서 제시하는 한국경제의 성장 ${\cdot}$ 고용 ${\cdot}$ 분배를 위한 정책방향은 다음과 같은 동태적발전과정으로 요약할 수 있다 : 기초과학연구능력 확충 ${\rightarrow}$ 소화 ${\cdot}$ 흡수 ${\cdot}$ 개량 ${\rightarrow}$ 토착화 능력의 배양 ${\rightarrow}$ 자체기술개발, 선진기술 도입, 산업간 및 산업내 기술확산, 국제기술협력 ${\rightarrow}$ 기술혁신의 촉진 ${\rightarrow}$ 총요소생산성과 기업경쟁력(자원 및 역량, 프로세스 경쟁력, 품질경쟁력, 시장경쟁력, 고객성과, 시장성과, 재무성과)의 제고 ${\rightarrow}$ 가격경쟁력(임금, 금리, 물류비용, 환율 등)과 비(非)가격경쟁력(디자인, 에프터서비스, 품질, 운송 등)의 제고 ${\rightarrow}$ 국가경쟁력의 제고 ${

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Fortified Antioxidative Potential by Chrysoeriol through the Regulation of the Nrf2/MAPK-mediated HO-1 Signaling Pathway in RAW 264.7 Cells (생쥐 대식세포에서 HO-1 발현 유도를 통한 chrysoeriol의 항산화 효과)

  • Park, Chung Mu
    • Journal of Life Science
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    • v.28 no.1
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    • pp.43-49
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    • 2018
  • Chrysoeriol is a widespread flavone, and it is usually found in alfalfa, which has been used as a traditional medicine to treat dyspepsia, asthma, and urinary system disorders. Recently, analysis has been conducted on the anti-inflammatory activity of chrysoeriol, but information on its antioxidative capacity is limited. In this study, the antioxidative potential of chrysoeriol against oxidative damage and its molecular mechanisms were evaluated by analysis of the cell viability, reactive oxygen species (ROS) formation, and Western blots in the RAW 264.7 cell line. Chrysoeriol significantly scavenged lipopolysaccharide (LPS)-induced intracellular ROS formation in a dose-dependent manner, without any cytotoxicity. Heme oxygenase-1 (HO-1), a phase II enzyme that exerts antioxidative activity, was also potently induced by chrysoeriol treatment, which corresponded to the translocation of nuclear factor-erythroid 2 p45-related factor 2 (Nrf2) into the nucleus. Moreover, mitogen-activated protein kinase (MAPK) and phosphoinositide 3-kinase (PI3K) were analyzed due to their important role in maintaining cellular redox homeostasis against oxidative stress. As a result, chrysoeriol-induced HO-1 upregulation was mediated by extracellular signal - regulated kinase (ERK), c-Jun $NH_2$-terminal kinase (JNK), and p38 phosphorylation. To identify the antioxidative potential exerted by HO-1, tert-butyl hydroperoxide (t-BHP)-induced oxidative damage was applied and mitigated by chrysoeriol treatment, which was confirmed by the HO-1 selective inhibitor and inducer, respectively. Consequently, chrysoeriol strongly strengthened the HO-1-mediated antioxidative potential through the regulation of the Nrf2/MAPK signaling pathways.

Characteristics of Fermented Dropwort Extract and Vinegar Using Fermented Dropwort Extract and Its Protective Effects on Oxidative Damage in Rat Glioma C6 Cells (미나리 발효액과 미나리 발효액을 이용한 식초의 특성 분석 및 glioma C6 세포에서 산화적 손상에 대한 보호 효과)

  • Kim, Min-Ju;Lee, Sam-Pin;Choi, Jun-Hyeok;Kwon, Seung-Hyuk;Kim, Hyung-Dae;Bang, Myun-Ho;Yang, Seun-Ah
    • Korean Journal of Food Science and Technology
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    • v.45 no.3
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    • pp.350-355
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    • 2013
  • The quality of fermented dropwort extract (FDE) and fermented dropwort vinegar (FDV) was assessed for free sugar, organic acid and free and total amino acid content. Major organic acids were lactic acid in FDE and acetic acid in FDV. Free sugars in FDE were fructose and glucose, and those in FDV were fructose, sucrose, and maltose. Aspartic acid was the major free amino acid in both FDE and FDV. Additionally, the main free amino acids in FDE were alanine and ${\gamma}$-amino-n-butyric acid (GABA), while those in FDV were arginine and valine. Moreover, to investigate the protective effects of FDE and FDV against oxidative stress induced by t-BHP and $H_2O_2$, C6 cells were treated with FDE or FDV prior to inducing the oxidative damage. FDE and FDV inhibited cell death significantly in a dose-dependent manner. These data imply that FDE and FDV may be effective in neuronal cell protection against oxidative damage.

Effect of Scutellaria Baicalensis Georgi. Extract on Cisplatin-Induced Acute Renal Failure in Rabbits (토끼에서 cisplatin에 의해 유도된 급성 신부전시 황금(黃芩; Scutellaria Baicalensis Georgi.) 추출물의 효과)

  • Kim, Soo-Chang;Song, Yeong-Min;Lee, Sung-Dae;Song, Seung-Hee;Koh, Phil-Ok;Kim, Jong-Su;Kim, Chung-Hui;Kang, Chung-Boo
    • Journal of Veterinary Clinics
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    • v.24 no.3
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    • pp.392-399
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    • 2007
  • Scutellaria baicalensis Georgi. (SBGE) is known to be antioxidant effect. In addition of the effects, we further investigated the SBGE on the antioxidant effect on a renal cortical slices cell and kidney protecting effects. The results were as follows. 1 When renal cortical slices separated from a rabbit's kidney were treated with 1mM tert-Butylhydroperoxide (t-BHP) in the presence of SBGE. SBGE significant prevented t-BHP induced increase in lactate dehydrogenase (LDH) release and lipid peroxidation. 2. When renal cortical slices separated from a rabbit's kidney were treated with oxidant $300{\mu}M$ cisplatin in the presence of SBGE. SBGE significant prevented cisplatin-induced increase in LDH release and lipid peroxidation. 3. Pretreatment with 0.1g/kg SBGE for seven day and treatment with 5 mg/kg cisplatin by the intraperitoneal injection The results were that the pretreatment group with SBGE showed a significant decrease in lipid peroxidation, increase in clearance rate of blood urea nitrogen (BUN) and creatinine in the kidney than the administering single agent group of cisplatin. and pretreatment group with SBGE showed intact microvillus of proximal tubule and no contraction of rumen, it was a similar result with normal group. With the results SBGE showed to be highly effective on antioxidant effect and cellular protection activity against cisplatin that was a toxic agent on a kidney. Therefore, SBGE is considered to have protective effective on a disordered kidney or kidney diseases such as nephritis or renal failure that cause tissue damages in a kidney.

Effect of Juglandis Semen Herbal Acupuncture on Toxic Agent-Induced Liver Cell Damage ((호도약침액)胡桃藥鍼液 독성물질(毒性物質)에 의한 간조직(肝組織) 손상(損傷)에 미치는 영향(影響))

  • Lee, Kyung-Tae;Kim, Cheol-Hong;Youn, Hyoun-Min;Jang, Kyung-Jeon;Ahn, Chang-Beohm;Song, Choon-Ho
    • Korean Journal of Acupuncture
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    • v.22 no.1
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    • pp.117-132
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    • 2005
  • Objectives : This study was carried out to determine whether Juglandis Semen herbal acupuncture (JSA) exerts the protective effect against toxic agent-induced live. cell damage. Methods : The cell damage was estimated by measuring lactate dehydrogenase (LDH) release, and lipid peroxidation was estimated by measuring maiondialdehyde (MDA), a product of lipid peroxidation, in rabbit liver slices. Results : When tissues were incubated with 0.5 mM Hg for $10{\sim}120\;min$, LDH release and lipid peroxidation were increased as a function of incubation time, and these effects were significantly prevented by addition of 0.1% JSA. Hg increased LDH release and lipid peroxidation in dose-dependent manner over the range of $0.1{\sim}l\;mM$ concentrations, which were reduced by 0.1% JSA. When tissues were treated with 0.5 mM Hg in the presence of $0.05{\sim}l\;%$ JSA, LDH release and lipid peroxidation induced by Hg were prevented by JSA in a dose-dependent fashion. JSA at 0.5 and 1% prevented completely effects of 0.5 mM Hg. When tissues were treated with 0.5 mM Hg for 60 min, LDH release and lipid peroxidation were increased, which were significantly prevented by addition of 0.1 % JSA. tert-Butyl hydroperoxide (tBHP) increased LDH release and lipid peroxidation, which were significantly reduced by 0.1 % JSA. Such protective effects were similar to those of N,N'-diphenyl-p-phenylenediamine (DPPD), a potent antioxidant. When tissues were treated with 0.5 mM Hg, activities of catalase and glutathione peroxidase were inhibited, and glutathione content was also reduced. Such effects were prevented by JSA, but not by DPPD. JSA prevented Hg-induced morphological changes. Conclusions : These results indicate that JSA exerts the protective effect against liver cell injury induced by toxic agents through antioxidant action, and this effect may be attributed to an increase in activities of endogeous anitoxidant enzymes and GSH content. However, antioxidant effect of JSA is different from that of a well-known potent antioxidant DPPD.

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