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Inhibition of in Vitro Growth of Three Soil-borne Turfgrass Diseases by Antagonistic Bacteria from Composted Liquid Manure (가축분뇨액비의 길항미생물에 의한 토양전염성 병원균의 생육억제 효과)

  • Ryu, Ju Hyun;Shim, Gyu Yul;Kim, Ki Sun
    • Horticultural Science & Technology
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    • v.32 no.6
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    • pp.879-886
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    • 2014
  • This study was conducted to test in vitro the antagonistic effect of composted liquid manure (CLM) against soil-borne turfgrass pathogenic fungi, Rhizoctonia solani AG-2-2 (IIIB) (brown patch), R. solani AG-2-2 (IV) (large patch), and Sclerotinia homoeocarpa (dollar spot) for environmentally friendly turfgrass management. CLMs were collected from 9 livestock excretion treatment facilities around the country including Gunwi (GW), Hapcheon (HC), Hoengseong (HS), Icheon (IC), Iksan (IS), Muan (MA), Nonsan (NS), and Yeoju (YJ). CLMs of IC, GW, and IS showed s ignificant (p < 0.05) mycelium growth inhibition that was 17.8%, 20.4%, and 48.0% against R. solani AG-2-2 (IIIB), R. solani AG-2-2 (IV), and S. homoeocarpa, respectively. A t otal of 110 bacterial isolates were obtained from the CLMs that showed antagonistic effects. Among them, 5, 4, and 10 microbe isolates showed promising antifungal activity against mycelium growth of R. solani AG-2-2 (IIIB), R. solani AG-2-2 (IV), and S. homoeocarpa, respectively. The bacterial isolates ICIIIB60, GWIV70, and ISSH20 effectively inhibited the mycelial growth of three soil-borne turfgrass pathogens. Selected bacterial isolates were identified as Alcaligenes sp., Bacillus licheniformis Ab2, and B. subtilis C7-3 through 16s rDNA gene sequence analysis. Among 5 fungicides, the most compatible fungicide with ICIIIB60, GWIV70, and ISSH20 was tebuconazol, toclofos-methyl and toclofos-methyl, respectively. These findings suggested that CLMs could be effectively used not only as organic liquid fertilizer sources but also as biological control agents for soil-borne turfgrass diseases such as brown patch, large patch, and dollar spot.

Acer tegmentosum Maxim Prevents Bone Loss by Inhibiting Osteoclastogenesis and Promoting Osteoblast Mineralization in Ovariectomized Mice

  • Oh, Tae Woo;Park, Kwang-Il;Do, Hyun Ju;Kim, Kyungho;Yang, Hye Jin;Cho, Won Kyung;Ma, Jin Yeul
    • Natural Product Sciences
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    • v.26 no.1
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    • pp.83-89
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    • 2020
  • Osteoporosis is a worldwide disease leading to significant economic and societal burdens globally. Osteoporosis is caused by unbalanced bone remodeling between the rate of osteoclast bone resorption and osteoblast bone formation. Acer tegmentosum Maxim (AT) is a traditional herbal medicine containing multiple biological activities such as anti-oxidant and anti-inflammatory purposes. However, its role in osteoporosis has not been fully studied. Therefore, we investigated whether AT has a potent inhibitory effect on osteoporosis and its mechanism through a systemic evaluation in ovariectomized (OVX) mice. OVX mice were orally administrated with the AT at doses of 50, 100, and 200 mg/kg for 10 weeks. Histological images and histomorphometry analyses were performed by H&E and Toluidine blue satin, and the expression levels of receptor activator for nuclear factor-kB ligand (RANKL), nuclear factor of activated T cells cytoplasm 1 (NFATc1), c-Fos, and matrix metalloproteinase 9 (MMP9) related to the osteoclast differentiation were investigated using immunohistochemical analysis. Administration of AT prevented bone loss and the alternations of osteoporotic bone parameters at the distinct regions of the distal femur and spongiosa region in OVX mice. Further, administration of AT increased periosteal bone formation in a dose-dependent manner. Meanwhile, AT inhibited not only the expression of NFATc1 and c-Fos, which are two major regulators of osteoclastogenesis but also reduced bone resorbed encoding expression of MMP9 and RANKL. Our results indicated that administration of AT prevented bone loss and the alternations of osteoporotic bone parameters at the distinct regions of the distal femur and spongiosa region in OVX mice. Also AT has the bone protective effect through the suppression of osteoclast and promotion of osteoblast, suggesting that it could be a preventive and therapeutic candidate for anti-osteoporosis.

The Experimental Phonetic Study of Word Accent in Standard Korean (표준한국어 악센트의 실험음성학적 연구 -청취 테스트 및 음향분석-)

  • Seong Cheol-jae
    • MALSORI
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    • no.21_24
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    • pp.43-89
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    • 1992
  • In this thesis, the prominent aspect of word accent in standard Korean is studied by auditory test and acoustic analysis experiment. The definition of 'accent' is, following Hoyoung Lee's discussion(1990), to be described as 'the means whereby a focused part of an utterance is made to stand out in order to concentrate the hearer's attention on it.' That is to say, the ten of 'accent' may be described in terms of phonological phenomenon and the accented syllable can be phonetically prominent as the result of those phonological process. Prosodic features may have different characteristics in different languages whether they contain linguistically important functions or not. Thus the characteristics of word accent in standard Korean will be determined as the content and trait of prosodic features. Following this viewpoint, present study looked over prosodic features which may effect the characteristics of word accent in standard Korean, through systematic experimental procedure. And the result of this experiment has been verified by statistical method, the T-test, for the purpose of identifying the relatedness among prosodic features(parameters). This thesis, therefore, aimed to investigate the intrinsic acoustic and physical qualities of the word accent in standard Korean. Nonsense words composed by 'mal' and 'ma' which can be divided into 'heavy syllable' and 'light syllable' quoted from Hyman(1975) have been classified into 28 types with respect to syllable numbers(2 syl., 3 sy1., 4 syl.) and these words have become the target of auditory test and acoustic experiment. As the result of those experimental Procedures, the word accent in standard Korean may be said that it has a tendency of fixing first two syllables regardless of syllable numbers. The syllable types of HH, HL, LL in the first two syllables may be prominent at first syllable and the type of H may be at second syllable. Various prosodic features(parameters) including duration, intensity, and Fo(purely phonetic terms) were also strengthened in those positions. The result of this experiment can be cleared up like these : 1. The most important feature is proved as 'duration', the feature of intensity resulted in more subsidiary one than the feature of duration. 2. Fo( fundamental frequency) could be observed as having some coherent contour through almost all syllable types(99 %), that is, in 2 syllable types, it had rising contour, in 2 syllable types, rising-falling contour, and in 4 syllable types, it contained rising-falling-rising contour. The result of auditory test was different with those contour forms of all Fo surveyed. With respect to these results, the discuss for Fo is determined' to be excluded comparing other features. 3. Finally, this thesis resulted in a decision that the word accent in standard Korean may has fixed(somewhat weaker) accent, especially fixed at first two syllables in almost all words. 4. Various kinds of syllable types related with 2,3,4 syllables, therefore, can be reclassified into 4 types of HH, HL, LH, LL following the concept of accent fixing placement(i.e. first two syllables). In these 4 types, the types of HH, HL, LL were prominent at the position of the first syllable , and the type of LH was prominent at the second syllable otherwise.

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Thermal Performance of Air Receiver filled with Porous Material for $5kW_t$ Dish Solar Collector (공기식 흡수기를 이용한 5kW급 접시형 태양열 집열기의 열성능 해석)

  • Seo, Joo-Hyun;Ma, Dae-Sung;Kim, Yong;Seo, Tae-Beom;Kang, Yong-Heack;Lee, Sang-Nam;Han, Gui-Young
    • Proceedings of the SAREK Conference
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    • 2007.11a
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    • pp.570-575
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    • 2007
  • The thermal performance of the air receiver filled with porous material for 5kWt dish solar collector installed in Inha University, Korea, is experimentally investigated. The diameter of the parabolic dish is 3.2 m, and its focal length is 2 m. It consists of 10 small pieces of glasses which have their own curvatures, and the effective reflecting area is 5.9 m2. The reflectivity of the glass is 0.95, and the thermal capacity of the system is about 5 kW thermal. The aperture diameter of the cylindrical-shape receiver which is made of stainless steel is 100 mm, and the height is 210 mm. A quartz window is installed at the receiver aperture to minimize the convective heat loss and prevent air leakages. In order to increase the heat transfer area, porous material (nickel-alloy) is inserted into the receiver. Air flows into the upper part of the receiver, which is the opposite side of the aperture. After the air flows through the inside receiver, that goes out of the receiver through 3 exits which are located near the aperture. The volumetric flow rates of air are varied from 600 to 1200 L/min. The thermal efficiency of the receiver ranges from 82% - 92% depending upon the flow rate. The results show that the system efficiency and receiver efficiency increase as the volume flow rate increases as expected. These results from the experiment will be useful for the applications to air heating receivers and solar reactors.

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Analysis of Bioconversion Components of Fermentation Hwangryunhaedok-tang (발효 황련해독탕의 생물 전환 성분분석)

  • Lee, Kwang Jin;Lee, BoHyoung;Jung, Pil Mun;Lian, Chun;Ma, Jin Yeul
    • YAKHAK HOEJI
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    • v.57 no.4
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    • pp.293-298
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    • 2013
  • Hwangryunhaedok-tang (HRT) is a traditional herbal medicine, which has been known as a useful prescription for anti-biotic, anti-inflammatory, anti-oxidative and immunosuppressive activity. In this study, the variation in the amount of eight bioactive components of Hwangryunhaedok-tang (HRT) and its fermentation HRT with Lactobacillus casei KFRI 127, Lactobacillus curvatus KFRI 166 and Lactobacillus confuses KFRI 227 was investigated via high-performance liquid chromatography coupled with diode array detection (HPLC-DAD). Simultaneous qualitative and quantitative analysis of eight bioactive components; geniposide, genipin, baicalin, wogonoside, palmatine, berberine, baicalein and wogonin was achieved by comparing their retention times ($t_R$) and UV spectra with those of the standard components. All calibration curve of standard components showed good linearity ($r^2$ >0.979). As a result, the geniposide amount was $15.52{\pm}0.19{\mu}/mg$ that as a main components in HRT. The wogonoside was decreased by 29.28~58.35% with Lactobacillus casei KFRI 127 and L. confuses KFRI 227 ($3.17{\pm}0.31{\mu}g/mg$ and $3.55{\pm}0.13{\mu}g/mg$) compared with the original HRT ($5.02{\pm}0.14{\mu}g/mg$). Otherwise wogonin was increased by 16.28~41.86% with Lactobacillus casei KFRI 127 and L. confuses KFRI 227 ($0.61{\pm}0.01{\mu}g/mg$ and $0.50{\pm}0.02{\mu}g/mg$) compared with the original HRT($0.43{\pm}0.00{\mu}g/mg$). HRT fermented with L. casei KFRI 127 and L. confuses KFRI 227 were evaluated as creating the changes in wogonoside to that aglycon wogonine. In the fermented HRT using Lactobacillus acidophilus KFRI 166, the genipin was only detected, among 3 species of fermentation strains. Thus, these results considered that the strains 166 were exhibited the remarkable changes in genipin.

Study on Encapsulation Efficiency and Preparation of SLN Using Conjugated New Whitening Agent(LA-PEG) and Its Cosmetic Application (새로운 미백물질을 함유하는 SLN의 제조와 봉입효율에 관한 연구)

  • Kang, Ki-Choon;Pyo, Hyeong-Bae;Lee, Cheong-Hee;Kim, Tae-Hoon;Ma, Sang-Chol;Im, Won-Cheol;Jeong, Noh-Hee
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.34 no.1
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    • pp.15-23
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    • 2008
  • This study is on encapsulation of a new whitening agent, LA-PEG using solid lipid nanoparticle(SLN) method, one of nanoparticle preparation method. Classical method has high capsulation efficiency for hydrophobic compounds but has demerit of low capsulation efficiency($2{\sim}3%$) for hydrophilic compounds. Purpose of this study is preparation of SLN that has higher skin penetration effect compared with general liposome, and also has higher encapsulation efficiency of hydrophilic compounds. For SLN preparation, coconut oil, macadamia oil, and jojoba oil were used. As a result, SLN preparation using coconut oil(include LA-PEG) has the most high encapsulation efficiency and also has the smallest average particle size(270 nm). SLN prepared with macadamia oil and 1% of Tween 60 has the largest particle size. Base made with coconut oil and 2% of Tween 60 showed the fastest release and base made with macadamia oil and 2% of Tween 20 showed the latest release.

A Comparison Research on the Expected Satisfaction and Actual Satisfaction on VOC of one General Hospital (서울소재 일개 종합병원의 CRM에 대한 기대만족도와 실제만족도 비교와 VOC활용)

  • Ma, Yeon-Ji;Kim, Jeong-Ah;Park, Seung-Woo;Oh, Eun-Hwa;Moon, So-Young;Rhee, Hyun-Sill
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.11 no.3
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    • pp.1146-1152
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    • 2010
  • The Korean healthcare industry is rapidly changing along with the competition among hospitals. In the past, hospitals could make profit without designing competitive management strategies. Thus, they did not find importance in listening to customers' voices and identifying their wants. However, nowadays, the increasingly intense competition is encouraging hospitals to seriously consider competitive management strategies and Customer Relationship Management (CRM) activities to gain a competitive advantage and prosper. It tries to compare the expected satisfaction with the satisfaction of out-patient and in-patient and analysis of VOC(Voice of Customer). This survey was done from 27th, April, 2009 to 8th May and each 100 in-patients and out-patients. The paired t-test and descriptive analysis was used to analysis between before and after satisfaction. The result, the replied out-patients were the highest of I.M department, 43% and in-patients, surgery and other department are the highest each 22.0%. Nurses kindness is statistical significant in out-patients. Doctor, Nurse and staff's kindness and rounding service was statistical significant in in-patients. Totally, the satisfaction was lower than expected satisfaction, so the medical care institutions should analyst detailed the patient's satisfaction by VOC.

The Effects of Spinal Decompression Combined with Therapeutic Modalities for Patients with Lumbar Radiculopathy (치료적 모달리티를 병용한 척추 감압치료가 요추 신경뿌리병증 환자에게 미치는 효과)

  • Ma, Sang-Yeol;Kwon, Won-An;Lee, Jae-Hong;Min, Dong-Gi
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.14 no.1
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    • pp.336-343
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    • 2013
  • The purpose of the present study was to determine the effect of 4 weeks course of motorized spinal decompression delivered via SpineMT(mobilization & traction) combined with therapeutic modalities on the treatment of patients with lumbar radiculopathy(LRP). A total of 15 patients with LRP (mean age, 36.63 years; age range 20-50years) participated in this study. 4 weeks course of spinal decompression delivered via SpineMT combined with therapeutic modalities was delivered to the patients for 6 days per week for the first two weeks, and four times per week for two additional weeks. The entire treatment consisted of 20 visits over 4 week period. Comparisons of changes in the muscle strengthening (MS), straight leg raise (SLR), and Oswestry disability index (ODI) at pre-intervention, after 10 treatment sessions, and at discharge (after 20 treatment sessions) were analyzed. There were significant improvements in the outcome measures of MS test, SLR test, and ODI score after 10 and 20 sessions of spinal decompression treatment combined with therapeutic modalities as compared with the pre-intervention(p<0.05). Spinal decompression treatment combined with therapeutic modalities appears to be a safe and efficacious, noninvasive treatment modality for patients with LRP.

Fabrication of long SmBCO coated conductor on IBAD-MgO template using co-evaporation method (동시증발법을 이용한 SmBCO/IBAD-MgO 박막 장선재 제조)

  • Ha, H.S.;Kim, H.S.;Ko, R.K.;Yoo, K.K.;Yang, J.S.;Kim, H.K.;Jung, S.W.;Lee, J.H.;Lee, N.J.;Kim, T.H.;Song, K.J.;Ha, D.W.;Oh, S.S.;Youm, D.;Park, C.;Yoo, S.I.;Moon, S.H.;Joo, J.
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2007.11a
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    • pp.241-241
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    • 2007
  • We fabricated SmBCO coated conductors(CCs) on IBAD-MgO templates using co-evaporation method. IBAD-MgO templates consist of PLD-LMO/epi-MgO/IBAD-MgO/Ni-alloy and showed good in-plane texture of below FWHM 7 degree. Evaporation rates of Sm, Ba, and Cu were precisely controlled to get the optimum composition ratio after deposition process. To optimize the oxygen partial pressure of reaction region, wide range of the partial pressure was investigated from 1 mTorr to 15 mTorr. By reducing the oxygen partial pressure to 5mTorr, (103)grains in SmBCO layer have been increased. On the other hand, there were only (001)grains in SmBCO layer deposited at 15 mTorr $O_2$. Deposition temperature was also investigated from $600^{\circ}C\;to\;800^{\circ}C$ to make high Ic SmBCO CCs. SmBCO on IBAD MgO template showed that the Ic increased gradually at higher growth temperature to $800^{\circ}C$, which the highest Jc and Ic is $2.6\;MA/cm^2$ and 500 A/cm-w., respectively.

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Petrogenesis of Mesozoic granites at Garorim Bay, South Korea: evidence for an exotic block within the southwestern Gyeonggi massif?

  • Kim, Ji In;Choi, Sung Hi;Yi, Keewook
    • Geosciences Journal
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    • v.23 no.1
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    • pp.1-20
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    • 2019
  • We present data from the Mesozoic Keumkang, Palbong, and Baekhwa granites in Garorim Bay, in the southwestern part of the Gyeonggi massif, South Korea. Using major and trace element concentrations, Sr-Nd-Pb isotopic compositions, and sensitive high-resolution ion microprobe (SHRIMP) zircon U-Pb ages, we aim to constrain the petrogenesis of the granites and explain their origin within a broader regional geological context. SHRIMP U-Pb zircon ages of $232.8{\pm}3.2$, $175.9{\pm}1.2$, and $176.8{\pm}9.8$ Ma were obtained from the Keumkang, Palbong and Baekhwa granites, respectively. The Late Triassic Keumkang granites belong to the shoshonite series and show an overall enrichment in large ion lithophile elements (LILE), a depletion in high field strength elements (HFSE) relative to primitive mantle, compared with neighboring elements in the primitive mantle-normalized incompatible trace element diagram with notable high Ba and Sr contents, and negligible Eu anomalies. The Keumkang granites are typified by highly radiogenic Sr and unradiogenic Nd and Pb isotopic compositions: $(^{87}Sr/^{86}Sr)_i=0.70931-0.70959$, $(^{143}Nd/^{144}Nd)_i=0.511472-0.511484$ [$({\varepsilon}_{Nd})_i=-17.0$ to -16.7], and $(^{206}Pb/^{204}Pb)=17.26-17.27$. The Middle Jurassic Palbong and Baekhwa granites belong to the medium- to high-K calc-alkaline series, and show LILE enrichment and HFSE depletion similar to the Keumkang granites, but exhibit significant negative anomalies in Ba, Sr, and Eu. Furthermore, they have elevated Y and Yb contents at any given $SiO_2$ content compared with other Jurassic granitoids from the Gyeonggi massif. The Palbong and Baekhwa granites have slightly less radiogenic Sr and more radiogenic Nd and Pb isotopic compositions [$(^{87}Sr/^{86}Sr)_i=0.70396-0.70908$, $(^{143}Nd/^{144}Nd)_i=0.511622-0.511660$, $({\varepsilon}_{Nd})_i=-15.4$ to -14.7, $(^{206}Pb/^{204}Pb)=17.56-17.76$] relative to the Keumkang granites. The Keumkang granites are considered to have formed in a post-collisional environment following the Permo-Triassic Songrim orogeny that records continent-continent collision between the North and South China blocks, and may have formed by fractional crystallization of metasomatized lithospheric mantle-derived mafic melts. The Palbong and Baekhwa granites may have been produced from a gabbroic assemblage at pressures of less than ~15 kbar, associated with subduction of the paleo-Pacific (Izanagi) plate at the Eurasian continental margin. Elevated ${\varepsilon}_{Nd}(t)$ values in the granitoids from the southwestern part of the Gyeonggi massif relative to those of the central and northern parts, together with the comparatively shallow depth of origin, imply the presence of an exotic block in the Korean lithosphere.