YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Analysis of Bioconversion Components of Fermentation Hwangryunhaedok-tang

발효 황련해독탕의 생물 전환 성분분석

  • Lee, Kwang Jin (Korean Institute of Oriental Medicine (KIOM), KM-Based Herbal Drug Development Group) ;
  • Lee, BoHyoung (Korean Institute of Oriental Medicine (KIOM), KM-Based Herbal Drug Development Group) ;
  • Jung, Pil Mun (Korean Institute of Oriental Medicine (KIOM), KM-Based Herbal Drug Development Group) ;
  • Lian, Chun (Korean Institute of Oriental Medicine (KIOM), KM-Based Herbal Drug Development Group) ;
  • Ma, Jin Yeul (Korean Institute of Oriental Medicine (KIOM), KM-Based Herbal Drug Development Group)
  • 이광진 (한국한의학연구원 한의신약개발그룹) ;
  • 이보형 (한국한의학연구원 한의신약개발그룹) ;
  • 정필문 (한국한의학연구원 한의신약개발그룹) ;
  • 량춘 (한국한의학연구원 한의신약개발그룹) ;
  • 마진열 (한국한의학연구원 한의신약개발그룹)
  • Received : 2013.06.12
  • Accepted : 2013.08.26
  • Published : 2013.08.31
Download PDF
⟨ Previous Next ⟩

Abstract

Hwangryunhaedok-tang (HRT) is a traditional herbal medicine, which has been known as a useful prescription for anti-biotic, anti-inflammatory, anti-oxidative and immunosuppressive activity. In this study, the variation in the amount of eight bioactive components of Hwangryunhaedok-tang (HRT) and its fermentation HRT with Lactobacillus casei KFRI 127, Lactobacillus curvatus KFRI 166 and Lactobacillus confuses KFRI 227 was investigated via high-performance liquid chromatography coupled with diode array detection (HPLC-DAD). Simultaneous qualitative and quantitative analysis of eight bioactive components; geniposide, genipin, baicalin, wogonoside, palmatine, berberine, baicalein and wogonin was achieved by comparing their retention times ($t_R$) and UV spectra with those of the standard components. All calibration curve of standard components showed good linearity ($r^2$ >0.979). As a result, the geniposide amount was $15.52{\pm}0.19{\mu}/mg$ that as a main components in HRT. The wogonoside was decreased by 29.28~58.35% with Lactobacillus casei KFRI 127 and L. confuses KFRI 227 ($3.17{\pm}0.31{\mu}g/mg$ and $3.55{\pm}0.13{\mu}g/mg$) compared with the original HRT ($5.02{\pm}0.14{\mu}g/mg$). Otherwise wogonin was increased by 16.28~41.86% with Lactobacillus casei KFRI 127 and L. confuses KFRI 227 ($0.61{\pm}0.01{\mu}g/mg$ and $0.50{\pm}0.02{\mu}g/mg$) compared with the original HRT($0.43{\pm}0.00{\mu}g/mg$). HRT fermented with L. casei KFRI 127 and L. confuses KFRI 227 were evaluated as creating the changes in wogonoside to that aglycon wogonine. In the fermented HRT using Lactobacillus acidophilus KFRI 166, the genipin was only detected, among 3 species of fermentation strains. Thus, these results considered that the strains 166 were exhibited the remarkable changes in genipin.

Keywords

References

  1. Zhang, H. Shen, P. and Cheng, Y. : Identification and determination of the major constituents in traditional Chinese medicine Si-Wu-Tang by HPLC coupled with DAD and ESIMS. J. Pharm. Biomed. Anal. 34, 705 (2004). https://doi.org/10.1016/S0731-7085(03)00650-2
  2. Jiang, W. Y. : Therapeutic wisdom in traditional Chinese medicine: a perspective from modern science. Trends. Pharmacol. Sci. 5, 455 (2005).
  3. Liu, S., Yi, L. Z. and Liang, Y. Z. : Traditional Chinese medicine and separation science. J. Sep. Sci. 31, 2113 (2008). https://doi.org/10.1002/jssc.200800134
  4. Kong, M. J., Ha, N. N., Lee, H. Y., Kim, Y. T., Rho, S. J. and Kim, H. C. : Effect of Hwangryunhaedok-tang and its modified prescription on the recovery of spatial cognitive function in the brain ischemia induced by four-vessel occlusion in rats. Kor. J. Herbal. 19, 161 (2004).
  5. Hwang, Y. S., Shin, C. Y., Huh, Y. and Ryu, J. H. : Hwangryun- Hae-Dok-tang (Huanglian-Jie-Du-Tang) extract and its constituents reduce ischemia-reperfusion brain injury and neutrophil infiltration in rats. Life Sci. 71, 2105 (2002). https://doi.org/10.1016/S0024-3205(02)01920-3
  6. Kim, S. G., Poudel, A. and Kim, Y. K. : Development of simultaneous analysis for marker constituents in Hwangryunhaedok-tang (黃連解毒湯) and its application in commercial herbal formulas. J. Nat. Med. 67, 390 (2013). https://doi.org/10.1007/s11418-012-0687-2
  7. Yang, H. J., Won, J. B., Lee, B. H. and Ma, C. J. : The alteration of components in the fermented Hwangryunhaedok-tang and its neuroprotective activity. Pharmacogn. Mag. 7, 207 (2011). https://doi.org/10.4103/0973-1296.84234
  8. Doh, E. S., Chang, J. P., Lee, K. H. and Seong, N. S. : Ginsenoside change and antioxidation activity of fermented ginseng. Korean J. Med. Crop. Sci. 18, 255 (2010).
  9. Hyun, J. S., Kang, S. M., Han, S. W., Kang, M. C., Oh, M. C. and Oh, C. K. : Flavonoid component changes and antioxidant activities of fermented Citrus grandis Osbeck Peel. J. Kor. Soc. Food. Sci. Nutr. 38, 1310 (2009). https://doi.org/10.3746/jkfn.2009.38.10.1310
  10. Nakano, D., Kwak, C. J., Fuji, K., Ikemura, K., Satake, A. and Ohkita, M. : Sesamin metabolites induce an endothelial nitric oxide-dependent vasorelaxation through their antioxidative property-independent mechanisms: possible involvement of the metabolites in the antihypertensive effect of sesamin. J. Pharmacol. Exp. Ther. 318, 328 (2006). https://doi.org/10.1124/jpet.105.100149
  11. Lin, Y. W. and Chiang, B. H. : Anti-tumor activity of the fermentation broth of Cordyceps militaris cultured in the medium of Radix astragali. Process Biochem. 43, 244 (2008). https://doi.org/10.1016/j.procbio.2007.11.020
  12. Park, H. Y., Um, Y. R. and Ma, J. Y. : Quantitative analysis of marker substance in fermented Rehmanniae Radix Preparata. Korean J. Pharmacogn. 41, 58 (2010).
  13. Maraza, J. A., Garro, M. S. and Giori, G. S. : Aglycone production by Lactobacillus rhamnosus CRL981 during soymilk fermentation. Food Microbiol. 26, 333 (2009). https://doi.org/10.1016/j.fm.2008.11.004
  14. Xu, M., Sun, Q., Su, J., Wang, J., Xu, C., Zhang, T. and Sun, Q. : Microbial transformation of geniposide in Gardenia jasminoides Ellis into genipin by Penicillium nigricans. Enzyme Microb. Technol. 42, 440 (2008). https://doi.org/10.1016/j.enzmictec.2008.01.003
  15. Chen, C., Han, F., Zhang, Y., Lu, J. and Shi, Y. : Simultaneous determination of geniposide and its metabolites genipin and genipinine in culture of Aspergillus unger by HPLC. Biomed. Chromatogr. 22, 753 (2008). https://doi.org/10.1002/bmc.993
  16. Ham, S. P., Lim, B. L., Ka, J. H., Ka, S. O. and Park, B. H. : Fermentation increase antidiabetic effects of Aconthopanax Senticosus. Kor. J. Ori. Med. Physiol. Pathol. 22, 340 (2008).
  17. Bae, E. A., Han, M. J., Kim, E. J. and Kim, D. H. : Transformation of ginseng saponins to ginsenoside Rh2 by acids and human intestinal bacteria and biological activities of their transformants. Arch. Pharm. Res. 27, 61 (2004). https://doi.org/10.1007/BF02980048
  18. Akao, T. and Kobashi, K. : Glycyrrhizin beta-D-glycyronidase of Eubacterium sp. From human intestinal flora. Chem. Pharm. Bull (Tokyo) 35, 205 (1987).