• 한국건설관리학회논문집
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• 제13권1호
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• pp.24-35
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• 2012

RFID 기반의 물류 관리는 건설 프로젝트의 효과적인 물류 관리를 위해 적용이 모색되는 기술로, 점차 다양한 분야로 기술 적용이 확대되고 있다. 하지만 기존의 연구들에서 지적하고 있는 바와 같이, RFID 기술이 가지는 잠재적 가능성에 비해 기술의 확산이 미흡한 상태에 있고, 이를 위한 적용 효과에 대한 검증이 관리자의 생산성 향상에 치우쳐져 있어, 보다 폭넓은 관점에서의 효과 검증이 필요한 상태에 있다. 따라서 본 연구에서는 RFID 기반의 물류관리 시스템의 보다 정확한 효과 검증을 위해, 실제 프로젝트의 커튼월 공사를 대상으로 시스템을 적용/운영하고, 현장 및 시스템 모니터링을 통해 적용효과 및 시사점을 제시하였다. 그리고 본 연구에서 제시한 RFID 기반 물류 관리 시스템의 적용효과는 기존 연구에서 제시하고 있는 관리자의 업무 시간 단축 및 생산성 향상과 관련된 효과뿐만 아니라, 물류 및 작업 생산성에 영향을 미치는 자원 평준화 측면에서의 효과를 포함하고 있으며, 기존 연구와는 달리 가장 유사한 환경을 가지는 쌍둥이 빌딩을 대상으로 RFID 적용 및 미적용 사례를 분석하여, 보다 정확한 비교 데이터를 제시하였다는데 의의를 가진다.

• 한국통신학회논문지
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• 제37권2C호
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• pp.132-139
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• 2012

2011년 배우식 등은 해시함수 기반의 새로운 저비용 RFID 상호인증 프로토콜(NLMAP)을 제안하였다. 그들은 난수 발생 등 연산량을 최소화하며, 상호인증으로 재전송 공격, 스푸핑 공격, 트래픽 공격, 도청 공격 등에 안전하기 때문에 RFID 시스템에 적용 시 제작비의 절감 및 보안성이 우수한 장점이 있다고 주장하였다. 그러나 그들의 주장과는 달리 도청 공격으로 해시된 태그의 고유 식별 정보 H(IDt)를 획득할 수 있다. 그리고 공격자가 획득한 H(IDt)를 이용하여 위치추적과 스푸핑 공격이 가능함을 본 논문에서 증명한다. 더 나아가 해시함수와 CRC코드를 이용한 상호인증 프로토콜을 제안한다. 그리고 제안 프로토콜이 NLMAP보다 다양한 공격에 안전하고 연산량 측면에서 효율적임을 증명한다.

• 한국통신학회논문지
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• 제35권3B호
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• pp.462-473
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• 2010

RFID(Radio-Frequency IDentification) 시스템은 개인 정보의 노출 및 위치 추적과 같은 보안상 문제점을 가지고 있다. 이러한 문제점을 해결하기 위해 해쉬함수와 같은 암호학적 접근방법들은 안전성을 증명하고 있지만 태그의 연산능력과 저장 공간의 한계로 인해 현실적으로 적용하기 어렵다. 최근의 경량 인증기법들은 단순 연산자만을 사용하여 높은 효율성을 보장하지만 안전성에 관한 주장이 충분하지 못하다. 본 논문에서는 안전한 RFID 인증을 위해서 대칭키 방식의 AES(Advanced Encryption Standard)를 이용하며 대칭키 방식에서의 고정된 키를 사용하던 문제를 단계적인 키 변환을 통해 해결한다. 프로토콜에서 태그와 서버의 동일한 대칭키는 태그, 리더, 백-엔드-서버에서 각각 생성된 난수를 이용하여 차례로 변환되며 태그와 리더의 출력 값을 항상 변경한다. 이와 같이 단계적으로 변환된 키를 이용할 경우 태그 정보의 노출 문제를 해결하며 도청, 재전송, 위치추적 및 스푸핑과 같은 공격에도 안전한 상호 인증이 이루어진다.

• Animal cells and systems
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• 제15권3호
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• pp.227-233
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• 2011

The Nramp1/Slc11a1 locus encodes a proton-coupled divalent cation transporter, expressed in late endosomes/lysosomes of macrophages, that constitutes a component of the innate immune response to combat intracellular pathogens and it was shown to play an important role in regulating inherent immunity. The previously identified Z-DNA forming polymorphic repeat(GT)n in the promoter region of the human Nramp1 gene does act as a functional polymorphism influencing gene expression. Research has shown that INF-${\gamma}$, TNF-${\alpha}$, IL-$1{\beta}$ and bacteria LPS increase the level of Nramp1 expression. However, the molecular mechanism for Nramp1 gene regulation is unclear. In this research, bovine Nramp1 5'-flanking region (-1748~+769) was cloned and analyzed by bioinformatics. Then to find the core promoter and the cis-acting elements, deletion analysis of promoter was performed using a set of luciferase reporter gene constructs containing successive deletions of the bovine Nramp1 5'-flanking regions. Promoter activity analysis by the dual luciferase reporter assay system showed that the core promoter of Nramp1 was located at +58~-89 bp. Some positive regulatory elements are located at -89~-205 bp and -278~-1495 bp. And the repressor elements were in region -205~-278 bp, intron1 and -1495~-1748 bp. LPS-responsive regions were located at -1495~-1748 bp and -278~-205 bp. The present study provides an initial effort to explore the molecular mechanism of transcriptional activation of the bovine Nramp1 gene and should facilitate further studies to decode the complex regulatory process and for molecular breeding for disease resistance in bovines.

• Smart Structures and Systems
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• 제16권3호
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• pp.415-433
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• 2015

Tendon failures in bonded post-tensioned bridges over the last two decades have motivated ongoing investigations on various aspects of unbonded tendons and their monitoring methods. Recent research shows that change of strain distribution in anchor heads can be useful in detecting wire breakage in unbonded construction. Based on this strain variation, this paper develops a damage detection model that enables an automated tendon monitoring system to identify and locate wire breaks. The first part of this paper presents an experimental program conducted to study the strain variation in anchor heads by generating wire breaks using a mechanical device. The program comprised three sets of tests with fully populated 19-strand anchor head and evaluated the levels of strain variation with number of wire breaks in different strands. The sensitivity of strain variation with wire breaks in circumferential and radial directions of anchor head in addition to the axial direction (parallel to the strand) were investigated and the measured axial strains were found to be the most sensitive. The second part of the paper focuses on formulating the wire breakage detection framework. A finite element model of the anchorage assembly was created to demonstrate the algorithm as well as to investigate the asymmetric strain distribution observed in experimental results. In addition, as almost inevitably encountered during tendon stressing, the effects of differential wedge seating on the proposed model have been analyzed. A sensitivity analysis has been performed at the end to assess the robustness of the model with random measurement errors.

• 한국균학회소식:학술대회논문집
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• 한국균학회 2015년도 추계학술대회 및 정기총회
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• pp.35-35
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• 2015

Pleurotus eryngii has recently become a major cultivated mushroom; it uses tetrapolar heterothallism as a part of its reproductive process. Sexual development progresses only when the A and B mating types are compatible. Such mating incompatibility occasionally limits the efficiency of breeding programs in which crossing within loci-shared strains or backcrossing strategies are employed. Therefore, understanding the mating system in edible mushroom fungi will help provide a short cut in the development of new strains. We isolated and identified pheromone and receptor genes in the B3 locus of P. eryngii and performed a functional analysis of the genes in the mating process by transformation. A genomic DNA library was constructed to map the entire mating-type locus. The B3 locus was found to contain four pheromone precursor genes and four receptor genes. Remarkably, receptor PESTE3.3.1 has just 34 amino acid residues in its C-terminal cytoplasmic region; therefore, it seems likely to be a receptor-like gene. Real-time quantitative RT-PCR (real-time qRT-PCR) revealed that most pheromone and receptor genes showed significantly higher expression in monokaryotic cells than dikaryotic cells. The pheromone genes PEphb3.1 and PEphb3.3 and the receptor gene PESTE3.3.1 were transformed into P5 (A3B4). The transformants were mated with a tester strain (A4B4), and the progeny showed clamp connections and a normal fruiting body, which indicates the proposed role of these genes in mating and fruiting processes. This result also confirms that PESTE3.3.1 is a receptor gene. In this study, we identified pheromone and receptor genes in the B3 locus of P. eryngii and found that some of those genes appear to play a role in the mating and fruiting processes. These results might help elucidate the mechanism of fruiting differentiation and improve breeding efficiency.

• Nuclear Engineering and Technology
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• 제45권5호
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• pp.613-624
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• 2013

Multi-dimensional two-phase phenomena occur in many industrial applications, particularly in a nuclear reactor during steady operation or a transient period. Appropriate modeling of complicated behavior induced by a multi-dimensional flow is important for the reactor safety analysis results. SPACE, a safety analysis code for thermal hydraulic systems which is currently being developed, was designed to have the capacity of multi-dimensional two-phase thermo-dynamic phenomena induced in the various phases of a nuclear system. To validate the performance of SPACE, a two-dimensional two-phase flow test was performed with slab geometry of the test section having a scale of $1.43m{\times}1.43m{\times}0.11m$. The test section has three inlet and three outlet nozzles on the bottom and top gap walls, respectively, and two outlet nozzles installed directly on the surface of the slab. Various kinds of two-dimensional air/water flows were simulated by selecting combinations of the inlet and outlet nozzles. In this study, two-dimensional two-phase void fraction profiles were quantified by measuring the local gap impedance at 225 points. The flow conditions cover various flow regimes by controlling the flow rate at the inlet boundary. For each selected inlet and outlet nozzle combination, the water flow rate ranged from 2 to 20 kg/s, and the air flow rate ranged from 2.0 to 20 g/s, which corresponds to 0.4 to 4 m/s and 0.2 to 2.3 m/s of the superficial liquid and gas velocities based on the inlet port area, respectively.

• 대한조선학회논문집
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• 제52권1호
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• pp.88-95
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• 2015

For the frequency-domain spectral fatigue analysis, the probability density function of stress range needs to be estimated based on the stress spectrum only, which is a frequency domain representation of the response. The probability distribution of the stress range of the narrow-band spectrum is known to follow the Rayleigh distribution, however the PDF of wide-band spectrum is difficult to define with clarity due to the complicated fluctuation pattern of spectrum. In this paper, efforts have been made to figure out the links between the probability density function of stress range to the structural response of wide-band Gaussian random process. An artificial neural network scheme, known as one of the most powerful system identification methods, was used to identify the multivariate functional relationship between the idealized wide-band spectrums and resulting probability density functions. To achieve this, the spectrums were idealized as a superposition of two triangles with arbitrary location, height and width, targeting to comprise wide-band spectrum, and the probability density functions were represented by the linear combination of equally spaced Gaussian basis functions. To train the network under supervision, varieties of different wide-band spectrums were assumed and the converged probability density function of the stress range was derived using the rainflow counting method and all these data sets were fed into the three layer perceptron model. This nonlinear least square problem was solved using Levenberg-Marquardt algorithm with regularization term included. It was proven that the network trained using the given data set could reproduce the probability density function of arbitrary wide-band spectrum of two triangles with great success.

• Archives of Pharmacal Research
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• 제26권12호
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• pp.1047-1054
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• 2003

Molecular chaperones have a crucial role in the folding of nascent polypeptides in endoplasmic reticulum. Some of them are known to be sensitive to the modification by electrophilic metabolites of organic pro-toxicants. In order to identify chaperone proteins sensitive to alkyators, ER extract was subjected to alkylation by 4-acetamido-4 -maleimidyl-stilbene-2,2 -disulfonate (AMS), and subsequent SDS-PAGE analyses. Protein spots, with molecular mass of 160, 100, 57 and 36 kDa, were found to be sensitive to AMS alkylation, and one abundant chaperon protein was identified to be protein disulfide isomerase (PDI) in comparison with the purified PDI. To see the reactivity of PDI with cysteine alkylators, the reduced form ($PDI_{red}$) of PDI was incubated with various alkylators containing Michael acceptor structure for 30 min at $38^{\circ}C$ at pH 6.3, and the remaining activity was determined by the insulin reduction assay. Iodoacetamide or N-ethylmaleimide at 0.1 mM remarkably inactivated $PDI_{red}$ with N-ethylmaleimide being more potent than iodoacetamide. A partial inactivation of $PDI_{oxid}$ was expressed by iodoacetamide, but not N-ethylmaleimide (NEM) at pH 6.3. Of Michael acceptor compounds tested, 1,4-benzoquinone ($IC_{50}, 15 \mu$ M) was the most potent, followed by 4-hydroxy-2-nonenal and 1,4-naphthoquinone. In contrast, 1,2-naphthoquinone, devoid of a remarkable inactivation action, was effective to cause the oxidative conversion of $PDI_{red}$ to $PDI_{oxid}$. Thus, the action of Michael acceptor compounds differed greatly depending on their structure. Based on these, it is proposed that POI, one of chaperone proteins in ER, could be susceptible to endogenous or xenobiotic Michael acceptor compounds in vivo system.

• 한국음향학회지
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• 제33권6호
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• pp.420-426
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• 2014

본 논문에서는 충격성 잡음 환경 하에서 인접 투사 알고리즘(Affine Projection Algorithm, APA)의 안정성을 높이기 위해 제안된 인접 투사 부호 알고리즘(Affine Projection Sign Algorithm, APSA)의 수렴 속도를 향상시키기 위한 새로운 APSA을 제안하였다. APSA은 충격성 잡음에 안정적으로 동작하고 역행렬 연산을 요구하지 않는다는 장점을 가지고 있다. 제안된 알고리즘은 기존 알고리즘의 장점을 가지면서도 기존의 알고리즘보다 더 빠른 수렴속도를 갖는다. 기존의 알고리즘은 i번째 과거 입력 신호를 필터 갱신을 위해 사용되는 모든 입력 신호의 $l_2$-norm으로 정규화 하지만 제안된 알고리즘은 i번째 과거 입력 신호를 i번째 과거 입력 신호의 $l_2$-norm 만으로 정규화한다. 시스템 식별 환경에서 두 알고리즘의 성능을 비교하는 컴퓨터 모의 실험을 수행하여 제안된 알고리즘의 수렴속도가 기존의 알고리즘보다 개선되었음을 보였다.