• Biotechnology and Bioprocess Engineering:BBE
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• v.8 no.1
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• pp.1-8
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• 2003

With current developments in enzyme-catalyzed reactions and techniques available for rational redesign of natural biocatalysts, the enzymatic biosynthesis can become one of the most valuable Synthetic methods. Enzymatic regioselective catalysis in organic media has played a key role in pursuing asymmetric synthesis for active chiral compounds. Here, we shortly do-scribe some historical issues of the rapidly growing area, enzymatic catalysis in synthetic organic chemistry and then review researches that have been carried out in the regioselective enzymatic catalysis for the past two decades. An application of this technology to the modification of some potential target drug co m pound will be adios presented.

• Journal of Microbiology
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• v.33 no.2
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• pp.142-145
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• 1995

The pumb gene encoding a basic amino acid transport protein in Neurospora crassa could be cloned by using a mutant strain defective in pmb gene as a host strain, using a negative selection on the media containing amino acid analogue canavanine. To select positive transformants of the genes for cloning, an auxotrophic marker (his-2) was added to a pmb mutant strain by mating ; a triple mutant (pmn : pmb : his-2) was constructued by crossing a strain defective in basic amino acid transport system (# 1683-bat um 535 "A") to a double mutant strain defective in neutral amino acid transport and histidine production (mitrol : his-2 "a"). Crossing was performed on synthetic crossing (SC) media containing histidine. The pmn : pmb and pmn :pmb : his-2 strains were selected among the progeny colonies from crosses on plates containing 5- .mu.g/ml para-fluoro-phenylalanine (PFPA), 200 .mu.g/ml canavanine, and 500 .mu.g/ml histidine. The selected colonies were cultured on minimal media with or without histidine for discarding pmn : pmb strain, because the pmn : pmb : his -2 strain grows only on histidine containing media. The pmn :pmb : his-2 strain selected can be used as a host strain for the cloning of the pmb and the pmn genes from a Neurospora genomic library by means of positive selections.

• Bulletin of the Korean Chemical Society
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• v.25 no.12
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• pp.1877-1880
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• 2004

The simple and convenient determination of trace Hg(II) with its 2-mercaptobenzothiazole (MBT) complex in cetyltrimethylammonium bromide (CTAB) media has been studied. The UV-visible spectrum of Hg(II)-MBT complex in CTAB media had a good sensitivity and reproducibility. The Hg(II)-MBT complex in CTAB solution was very stable at pH 10.0 and could be quantitatively complexed if MBT were added to the sample solution more than 10 equivalent of Hg(II). The optimum concentration of CTAB was 0.001%. The calibration curve of Hg(II)-MBT complex with good linearity ($R^2$ = 0.9985) was obtained at the concentration range between $1.0{\times}10^-7$ and $1.0{\times}10^-5$ M in 0.001% CTAB media. The detection limit was $3.1{\times}10^-8$ M (6.2 ng m$L^{-1}$). Hg(II) in the synthetic samples and in the wastewater of the university's wastewater tank and the industrial wastewater tank could also be determined. Based on the experimental results, this proposed technique could be applied to the simple and convenient determination of trace Hg(II) in real samples.

• Proceedings of the Korean Society of Broadcast Engineers Conference
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• 2020.07a
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• pp.232-235
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• 2020

최근 딥러닝을 이용한 자동차 번호판 인식 알고리즘에 있어서 인조 번호판을 생성하여 데이터 수집과 라벨링 작업 시간을 줄이기 위한 연구가 진행되고 있다. 하지만 인조 번호판의 특성상 정면의 이미지로 구성되어 있기 때문에 자동차의 정면에서 촬영된 번호판의 인식률은 높지만 측면에서 촬영된 번호판의 경우 인식률이 낮아진다. 본 논문에서는 다양한 카메라 설치 위치에 따른 다각도로 촬영된 번호판 영상의 인식률을 보완하기 위해 이미지를 3차원으로 회전하여 데이터를 생성하는 인조 번호판 생성기 프로그램을 개발하였다. 3차원 회전을 하였을 때 번호판 인식 성능을 비교하기 위해 기존 방식으로 생성한 번호판과 제안 방식으로 생성한 번호판 각 600,000장씩 생성하여 총 1,200,000장을 생성하였으며, 데이터의 비율에 따라 10가지의 학습 데이터 셋을 구성하였다. 인조 번호판 데이터의 학습 결과를 평가하기 위해 실제 번호판 이미지 1789장으로 테스트 셋을 구성하였고, 기존의 인조 번호판 생성 방식과 인식 정확도를 비교 분석하였다.

• Journal of Broadcast Engineering
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• v.26 no.4
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• pp.453-462
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• 2021

A lot of license plate data is required for car number recognition. License plate data needs to be balanced from past license plates to the latest license plates. However, it is difficult to obtain data from the actual past license plate to the latest ones. In order to solve this problem, a license plate recognition study through deep learning is being conducted by creating a synthetic license plates. Since the synthetic data have differences from real data, and various data augmentation techniques are used to solve these problems. Existing data augmentation simply used methods such as brightness, rotation, affine transformation, blur, and noise. In this paper, we apply a style transformation method that transforms synthetic data into real-world data styles with data augmentation methods. In addition, real license plate data are noisy when it is captured from a distance and under the dark environment. If we simply recognize characters with input data, chances of misrecognition are high. To improve character recognition, in this paper, we applied the DeblurGANv2 method as a quality improvement method for character recognition, increasing the accuracy of license plate recognition. The method of deep learning for license plate detection and license plate number recognition used YOLO-V5. To determine the performance of the synthetic license plate data, we construct a test set by collecting our own secured license plates. License plate detection without style conversion recorded 0.614 mAP. As a result of applying the style transformation, we confirm that the license plate detection performance was improved by recording 0.679mAP. In addition, the successul detection rate without image enhancement was 0.872, and the detection rate was 0.915 after image enhancement, confirming that the performance improved.

• Journal of Pharmaceutical Investigation
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• v.22 no.3
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• pp.49-63
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• 1992

Two different types of chemical manipulations of dobutamine were investigated in order to develop novel, improved cardiotonic drugs. Three new analogues of carbostyril, in which the m-hydroxy group of dobutamine was isosterically modified with an amide type carbostyril system, were synthesized from, ${\rho}-methoxyphenethylamine$ via multi-steps. Two analogues of (2'-aminoethyl)-1-hydroxy-2-pyridone system which has isosteric structural similarity with dopamine without having the COMT vulnerable m-hydroxy group were synthesized via 12 synthetic steps. Their biological stabilities in various media and inotropic activities were evaluated.

• Proceedings of the Korean Society of Broadcast Engineers Conference
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• 1995.06a
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• pp.91-96
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• 1995

In this paper we propose algorithms for high resolution image sequence interpolation. Image sequences, in general, are assumed to have greater amount of information than a still image. By this reason, image sequences can be used to improve the resolution of interpolated image sequences. Therefore the proposed algorithms can be the theoretical basis for interpolating dynamic image sequences. In order to demonstrate the validity of the proposed algorithms, experimental results using both synthetic and real test images are presented.

• International Journal of Industrial Entomology and Biomaterials
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• v.5 no.2
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• pp.217-220
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• 2002

Mulberry being a foliage crop is grown extensively for feeding of silkworms and are also used for cattle feeding. These loaves are highly nutritious, which contain various mineral elements and bio-molecules such as carbohydrates, proteins, lipids and other essential amino acids, etc. In the present study, deproteinized mulberry leaf juice was used for preparation of the medium for cultivation of various types of microbes. Results revealed that deproteinized mulberry leaf juice medium is best for isolation of fungi, bacteria and actinomycetes and this medium can be substituted with synthetic media, which are haying the costly ingredients for isolation and identification of bacteria, fungi and actinomycetes. Further, this deproteinized mulberry juice medium can also be used in mass multiplication of useful/beneficial microbes to enhance soil microflora to improve soil fertility and to avoid root diseases. Perspective enterprises can take up the mass multiplication/large-scale production of useful microbes such as Trichoderma, Rhizobium, Pseudomonas and Bacillus to use in mulberry and in other agricultural crops using deproteinized mulberry leaf juice.

• Journal of the Korean Chemical Society
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• v.56 no.1
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• pp.58-61
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• 2012

The solvent extraction of platinum (IV) metal from hydrochloric acid media using 4-(4-ethoxybenzylideneamino)-5-methyl-4H-1,2,4-triazole-3-thiol (EBIMTT) in chloroform was studied as a function of several variables, such as reagent, acid and metal ion concentration, effect of various diluents, and diverse ions. The proposed method was further applied for the separation of platinum (IV) from binary mixtures, synthetic mixtures, alloys and commercially available samples.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.11 no.6
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• pp.3121-3142
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• 2017

We present a method for 3D shape reconstruction of inextensible deformable surfaces from a single image. The key of our approach is to represent the surface as a 3D triangulated mesh and formulate the reconstruction problem as a sequence of Linear Programming (LP) problems. The LP problem consists of data constraints which are 3D-to-2D keypoint correspondences and shape constraints which are designed to retain original lengths of mesh edges. We use a closed-form method to generate an initial structure, then refine this structure by solving the LP problem iteratively. Compared with previous methods, ours neither involves smoothness constraints nor temporal consistency, which enables us to recover shapes of surfaces with various deformations from a single image. The robustness and accuracy of our approach are evaluated quantitatively on synthetic data and qualitatively on real data.