• Journal of Environmental Science International
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• v.12 no.1
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• pp.87-92
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• 2003

Synchronous spectrofluorimetry was carried out for the simultaneous determination of various polycyclic aromatic hydrocarbons(PAHs) in aqueous solution by fluorescence spectrometry have been studied. The optimal wavelength interval(${\triangle}{\lambda}$) for synchronous spectra of acenaphthene, anthracene, benzo[a]anthracene, fluorene and pyrene were investigated in the presence of surfactants. The great enhancement of the fluorescence of these PAHs in Triton X-100 was obtained and optimal wavelength was 50 nm. The calibration curves in synthetic mixture solution of 5 PAHs were linear over the range from $1.0{\times}10^{-8}M$ to $1.0{\tiems}10^{-4}M$. Under the optimal experimental conditions, the detection limits were $4.9{\tiems}10^{-9}M$,\;7.0{\times}10^{-9}M,\;4.7{\tiems}10^{-9}M,\;1.6{\tiems}10^{-9}M$ and $3.2{\tiems}10^{-9}M$ for acenaphthene, anthracene, benzo[a]anthracene, fluorene and pyrene, respectively.

• Analytical Science and Technology
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• v.15 no.5
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• pp.393-398
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• 2002

A method to determine simultaneously fluorene and anthracene in aqueous solution by synchronous fluorescence spectrometry has been studied. The emission characteristics and optimum wavelength interval (${\Delta}{\lambda}$) for synchronous spectra of fluorene and anthracene in aqueous solution were investigated. The optimum wavelength interval (${\Delta}{\lambda}$) was found to be 50 nm. The calibration curve for fluorene and anthracene in the synthetic mixture solution of both compounds was linear over the range from $5.0{\times}10^{-8}M$ to $1.0{\times}10^{-3}M$ and from $5.0{\times}10^{-8}M$ to $1.0{\times}10^{-3}M$ for fluorene and anthracene, respectively. The detection limit was $3.0{\times}10^{-9}M$ and $7.0{\times}10^{-9}M$, for fluorene and anthracene, respectively under the optimal wavelength interval.

• Bulletin of the Korean Chemical Society
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• v.32 no.7
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• pp.2253-2259
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• 2011

The self-assembly of one novel organic complex based on chlorogenic acid (HCA) and 2,2'-bipyridine (2,2'-bipy) has been synthesized and characterized. The complex achieved by hydrogen-bonding interactions, adopted a 1:1 stoichiometry in a solid state. The proton transfer occurred from the carboxyl oxygen to the aromatic nitrogen atom to form salts CA${\cdot}$(2,2'-Hbipy), the 2,2'-Hbipy molecule individually occupies the pseudo-tetragonum that is formed with CA. In this paper, the interactions of CA${\cdot}$(2,2'-Hbipy) with bovine serum albumin (BSA) were studied by fluorescence spectrometry. For CA${\cdot}$(2,2'-Hbipy), HCA and 2,2'-bipy, the average quenching constants for BSA were $2.4384{\times}10^4$, $4.653{\times}10^3$, and $3.059{\times}10^3\;L{\cdot}mol^{-1}$, respectively. The mechanism for protein fluorescence quenching is apparently governed by a static quenching process. The Stern-Volmer quenching constants and corresponding thermodynamic parameters ${\Delta}$H, ${\Delta}$G and ${\Delta}$S were calculated. The binding constants and the number of binding sites were also investigated. The conformational changes of BSA were observed from synchronous fluorescence spectra.

• Analytical Science and Technology
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• v.16 no.5
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• pp.339-348
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• 2003

Determination of some PAHs in used engine oils have been carried out by extraction of the components into acetonitrile followed by GC/FID and synchronous spectrofluorimetric technique. 7 PAHs, such as acenaphthene (Ace), anthracene (Anth), benzo(a)pyrene (BaP), chrysene (Chry), phenanthrene (Phen), fluoranthene (Ft), and perlyrene (Per) in used engine oil sample were able to determine separately by synchronous spectrofluorimetry. Calibration curves for those components were linear for the concentration range of 0.4~166 ppb PAHs with the corelation factor of 0.9985~0.9999. The peak areas produced by GC/FID split ratio program were used for the calibration curves of the other 8 PAHs. Detection sensitivity of the synchronous spectrofluorimetry seems to be 100 times more sensitive than GC/FID method. The total amount of PAHs in the used engine oil were 5.5 ng/g for LNG (bus), 10.5 ng/g for LPG(taxi), 92.2 ng/g for gasoline-passenger car, and 130 ng/g for diesel trailer, respectively.