• KSBB Journal
• /
• 제17권1호
• /
• pp.33-37
• /
• 2002

PLGA 미립구로부터 G-CSF의 방출 거동 양상을 향상 시키기 위하여 G-CSF를 분자량 5000인 methoxy polyethylene glycol-aldehyde로 PEGylation 시켰다. 대부분의 G-CSF는 mono-PEGylation 되었으며, 이를 SDS-PAGE, HPLC, 및 펩타이드 지도 분석을 통해 확인하였다. W/O/W 방법을 사용하여 G-CSF 및 PEGylated G-CSF의 PLGA 미립구를 제조하였으며, 이때 봉입율은 높은 상태였다. 미립구내로 더 많은 G-CSF를 봉입하기 위하여 액상의 G-CSF 및 PEGylated G-CSF을 농축하였고 native gel과 gel filtration 크로마토 그래피를 통하여 단백질이 안정함을 확인하였다. 이렇게 제조한 PLGA 봉입체의 in vitro 방출 거동을 조사한 결과 PEGylated G-CSF는 native G-CSF에 비하여 더 오랜 시간 동안 방출이 지속되었고 최대 방출량도 증가하였다.

• 한국분말재료학회지
• /
• 제25권6호
• /
• pp.459-465
• /
• 2018

Most commercially available detonation nanodiamonds (DNDs) require further processing to qualify for use in biomedical applications, as they often contain many impurities and exhibit poor dispersibility in aqueous media. In this work, DNDs are modified to improve purity and impart a high colloidal stability to the particles. The dispersive and adsorption properties of modified DNDs are evaluated in terms of the suitability of DNDs as carriers for non-steroidal anti-inflammatory drugs (NSAIDs) in transdermal delivery. The study of adsorption on strongly positively and strongly negatively charged DNDs showed their high loading capacity for NSAIDs, and a pronounced relationship between the drugs and the particles' charges. Experiments on long-term desorption carried out with DND/NSAID complexes indicate that the nanoparticles exert a sustained effect on the drug release process.

• 한국진공학회:학술대회논문집
• /
• 한국진공학회 2016년도 제50회 동계 정기학술대회 초록집
• /
• pp.360.1-360.1
• /
• 2016

Hydroxyapatite (Ca10(PO4)6(OH)2, HAP) particles have attracted a great deal of attention in biomedical fields due to their good biocompatibility, bioactivity and fairly broad applications as drug delivery, dental implant, bone cement, and etc. Thus, many researchers have made an effort to add new functionalities such as luminescence, drug delivery, and bone regeneration properties up to HAP powders by controlling their nanostructure as well as composition. In this research, the mesoporous strontium substituted HAP (Sr-HAP) microspheres were synthesized using a hydrothermal method. In this synthesis, aspartic acid monomers were utilized to form microsphere by controlling surface energy of HAP particles and Sr ions were substituted into Ca ion sites, which induced luminescence property in HAP powders. Moreover, the change in the amount of Sr substitution was found to influence the particle size, morphology, and concurrently surface area, which led to changing drug loading as well as drug release property. The amount of Sr influences the morphology, luminescent properties, particle size, surface area cell viability and drug loading property, which are investigated by SEM, TEM, XRD, FTIR, BET, XPS and in vitro test such as MTT assay and drug release test. In particular, the multifunctional Sr-HAP with molar ratios of 0.25 (Sr/(Ca+Sr)) possessed the strongest luminescent property as well as the superior drug loading and sustained release properties that were correspondent with large surface area and pore size. Our study indicates that the fabricated multifunctional Sr-HAP microspheres are quite useful for bone regeneration and drug delivery.

• 한국지반공학회논문집
• /
• 제24권6호
• /
• pp.101-116
• /
• 2008

보강토 옹벽의 영구구조물로서의 적용성이 증가함에 따라 지속 혹은 반복하중 등 다양한 하중조건하에서 장기적인 잔류변형이 설계/시공시 주요 관점이 되고 있다. 본 연구에서는 교량구조물의 피어 및 Back-to-Back(BTB) 형태의 보강토 옹벽을 대상으로 사용하중 작용 이전에 작용시키는 선행하중이 보강토 구조물의 잔류변위를 제어에 미치는 영향을 고찰하였다. 이를 위해 모형 피어와 BTB 옹벽을 축조하여 단순재하, 지속하중, 반복하중 등 다양한 하중조건 및 시공조건에 대한 모형실험을 수행하였다. 그 결과 선행재하 공법은 다양한 하중 및 시공조건에서 잔류변위 제어에 탁월한 것으로 나타났다.

• Journal of Pharmaceutical Investigation
• /
• 제32권3호
• /
• pp.199-207
• /
• 2002

In order to develop a sustained release formulation of bovine somatotropin (BST), which has been used to increase the body weight of oxen or the milk production of dairy cows, poly(D,L-lactide-co-glyceride)(PLGA) microspheres were made by W/O/W multiple emulsification method and solvent extraction method. Physical properties including particle size, drug entrapment, drug release, protein denaturation, and in vivo body weight increase in rats were characterized. The size of the microspheres was increased as the molecular weight of PLGA increased. When Span 65 and stearic acid during preparation were added, the size was decreased but the amount of surface protein was increased, resulting in a high loading efficiency, with fast release of BST from the microspheres. Aggregation or fragmentation of BST by SDS-PAGE during microsphere preparation and drug release study was not observed. Body weight of Sprague-Dawley's male rats was significantly increased after subcutaneous administrations of BST-loaded PLGA microspheres. There was a good correlation between in vivo weight gain and in vitro release rate of microspheres. PLGA microspheres with a high surface protein ratio could be a good candidate for the sustained delivery of BST.

• 한국환경과학회지
• /
• 제9권6호
• /
• pp.449-454
• /
• 2000

Sustainable development has been the key concept, both in economic policies and in environmental management. On the basis of an systems ecology perspective, this suggests the sustainable development of Korea\`s natural environment and economic activity using the EMERGY evaluation. The total EMERGY use(7,730E20sej/yr) in Korea is 81 percent from imported sources, fuels and goods and services. The ratio of outside investment to attracting natural resources is already large, like other industrialized countries. Continued availability of purchased inputs at a favorable balance of EMERGY trade, currently about 2.85 to 1 net EMERGY, tis the basis for present economic activity and must decrease as the net EMERGY of purchased inputs including fossil fuels goes down. EMERGY yield ratio and environmental loading ratio were 1.23 and 20.30, respectively. The population level is already in excess of carrying capacity. Its carrying capacity for steady state on its renewable sources is only 2.2 million people, compared to 45.9 million in 1977. EMERGY sustainability index is therefore less than one, which is indicative of highly developed consumer oriented economies. Until now the development of a country has been achieved by the economic growth, but it can be sustained in the long run by the use of renewable resource systems. the efficiency of energy usage, and the transformation of the social-economic structure based on an ecological-recycling concept.

• Computers and Concrete
• /
• 제5권6호
• /
• pp.573-597
• /
• 2008

Among artificial intelligence-based computational techniques, adaptive neuro-fuzzy inference systems (ANFIS) are particularly suitable for modelling complex systems with known input-output data sets. Such systems can be efficient in modelling non-linear, complex and ambiguous behaviour of cement-based materials undergoing single, dual or multiple damage factors of different forms (chemical, physical and structural). Due to the well-known complexity of sulfate attack on cement-based materials, the current work investigates the use of ANFIS to model the behaviour of a wide range of self-consolidating concrete (SCC) mixture designs under various high-concentration sodium sulfate exposure regimes including full immersion, wetting-drying, partial immersion, freezing-thawing, and cyclic cold-hot conditions with or without sustained flexural loading. Three ANFIS models have been developed to predict the expansion, reduction in elastic dynamic modulus, and starting time of failure of the tested SCC specimens under the various high-concentration sodium sulfate exposure regimes. A fuzzy inference system was also developed to predict the level of aggression of environmental conditions associated with very severe sodium sulfate attack based on temperature, relative humidity and degree of wetting-drying. The results show that predictions of the ANFIS and fuzzy inference systems were rational and accurate, with errors not exceeding 5%. Sensitivity analyses showed that the trends of results given by the models had good agreement with actual experimental results and with thermal, mineralogical and micro-analytical studies.

• The Korean Journal of Physiology
• /
• 제10권2호
• /
• pp.23-28
• /
• 1976

Author have already reported that urinary aldosterone excretion of the Korean who usually eat high sodium diet is significantly lower comparing with the American, although the plasma aldosterone concentration is identical in the former with that of the latter. Measurement of urinary aldosterone excretion and Plasma concentration only is insufficient to establish the pressence and/or mode of evolution of the Korean. In this experiments, aldosterone secretion rate(ASR) was measured in normotensive Korean during high and low dietary sodium intake with or without additional potassium supply. Results were as follows; 1) In normal Korean, dietary sodium restriction resulted in appreciable increase in ASR, and a sustained increase in urinary aldosterone excretion with an increase in plasma level. 2) Oral potassium loading easily stimulated the adrenal cortex of the Korean who already adapted to a high sodium diet when dietary sodium is still identical with not·mal American. 3) Quantitative relationships between aldosterone secretion rate, plasma concentration and urinary excretion of aldosterone were altered by potassium loading. 4) Urinary aldosterone excretion didn't reflect concurrent increase aldosterone secretion in subjects with Potassium intake. It was discussed that the changes of tile relationships and of adrenal hyper response on Potassium Beading in the Korean will be elucidated by measuring the metabolic clearance rate.

• Macromolecular Research
• /
• 제15권6호
• /
• pp.547-552
• /
• 2007

Water soluble polymer, poly(vinyl pyrrolidone) was chosen to conjugate with 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(succinyl) (N-succinyl DPPE) to make a new drug delivery system. PVP with an amine group (amino-PVP) was polymerized by free radical polymerization. The amine group of amino-PVP was conjugated with the carboxylic group of N-succinyl DPPE. The resultant conjugate could form nanoparticles in the aqueous solution; these nanoparticles were termed a lipid-polymer system. The critical aggregation concentration was measured with pyrene to give a value of $1{\times}10^{-3}g/L$. The particle size of the lipid-polymer system, as measured by DLS, AFM and TEM, was about 70 nm. Lipophilic component in the inner part of the lipid-polymer system could derive the physical interaction with hydrophobic drugs. Griseofulvin was used as a model drug in this study. The loading efficiency and release profile of the drug were measured by HPLC. The loading efficiency was about 54%. The release behavior was sustained for a prolonged time of 12 days. The proposed lipid-polymer system with biodegradable and biocompatible properties has promising potential as a passive-targeting drug delivery carrier because of its small particle size.

• 한국지진공학회논문집
• /
• 제6권3호
• /
• pp.41-52
• /
• 2002

연계논문에서는 철근콘크리트 교각의 지진손상 평가를 위한 비선형 유한요소해석 기법을 제시하였다. 이 논문에서는 철근콘크리트 교각의 이력거동의 예측에 근거한 손상지수를 제시하였다. 손상지수는 지진하중하의 철근콘크리트 교각의 손상을 수치적으로 정량화하는 방법으로서 제안되었다. 제안한 해석기법을 실험된 철근콘크리트 교각에 적용하였고 다른 연구자의 손상지수와 비교.분석하였다. 제안된 해석기법은 조사된 실험체에 대하여 하중단계에 따라 손상을 정확하게 예측하였다.