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Sustained Release of PLGylated G-CSF from PLGA Microsphere  

정경환 ((재)목암생명공학연굿)
임형권 ((재)목암생명공학연굿)
이시욱 ((재)목암생명공학연굿)
강관엽 ((재)목암생명공학연굿)
박태관 (한국과학기술원 생물과학과)
Publication Information
KSBB Journal / v.17, no.1, 2002 , pp. 33-37 More about this Journal
Abstract
To improve in vitro release kinetic of G-CSF in PLGA microsphere, G-CSF was PEGylated with methoxy polyethylene glycol-aldehyde (mPEG-aldehyde, MW 5000). The majority of G-CSF was mono-PEGylated and it was characterized using SDS-PAGE, HPLC, and peptide mapping. The PLGA microencapsulation with the native, or PEGylated G-CSF was performed using W/O/w method, where the encapsulation efficiency was high. For the high loading of G-CSF to microsphere, G-CSF and PEGylated G-CSF were concentrated and then verified the protein stability using native gel and gel filtration chromatography. In comparison with native G-CSF, PEGylated G-CSF was released during the extended period and its maximum amount of released G-CSF was also increased.
Keywords
G-CSF; PEGylation; PLGA microencapsulation; Sustained release;
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Times Cited By KSCI : 1  (Citation Analysis)
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