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http://dx.doi.org/10.4333/KPS.2002.32.3.199

Sustained Release Injectable of Recombinant Bovine Somatotropin in Biodegradable Poly(D,L-lactide-co-glyceride) Microspheres  

Jeon, Hong-Ryeol (College of Pharmacy, Chung-Ang University)
Lee, Bong-Sang (College of Pharmacy, Chung-Ang University)
Kown, Do-W (College of Pharmacy, Chung-Ang University)
Yoon, Mi-Kyoung (College of Pharmacy, Chung-Ang University)
Jeon, Hyun-Joo (College of Pharmacy, Chung-Ang University)
Shin, Taek-Hwan (College of Pharmacy, Chung-Ang University)
Choi, Young-Wook (College of Pharmacy, Chung-Ang University)
Publication Information
Journal of Pharmaceutical Investigation / v.32, no.3, 2002 , pp. 199-207 More about this Journal
Abstract
In order to develop a sustained release formulation of bovine somatotropin (BST), which has been used to increase the body weight of oxen or the milk production of dairy cows, poly(D,L-lactide-co-glyceride)(PLGA) microspheres were made by W/O/W multiple emulsification method and solvent extraction method. Physical properties including particle size, drug entrapment, drug release, protein denaturation, and in vivo body weight increase in rats were characterized. The size of the microspheres was increased as the molecular weight of PLGA increased. When Span 65 and stearic acid during preparation were added, the size was decreased but the amount of surface protein was increased, resulting in a high loading efficiency, with fast release of BST from the microspheres. Aggregation or fragmentation of BST by SDS-PAGE during microsphere preparation and drug release study was not observed. Body weight of Sprague-Dawley's male rats was significantly increased after subcutaneous administrations of BST-loaded PLGA microspheres. There was a good correlation between in vivo weight gain and in vitro release rate of microspheres. PLGA microspheres with a high surface protein ratio could be a good candidate for the sustained delivery of BST.
Keywords
Bovine somatotropin; PLGA; Microsphere; Drug release; Weight increase;
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