• Bulletin of the Korean Chemical Society
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• v.34 no.8
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• pp.2408-2412
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• 2013

We demonstrate herein the synthesis and modification of magnetic nanoparticles and its use in the immobilization of the lipase. Magnetic $Fe_3O_4$ nanoparticles (MNPs) were prepared by simple co-precipitation method in aqueous medium and then subsequently modified with tetraethyl orthosilicate (TEOS) and 3-aminopropyl triethylenesilane (APTES). Silanization magnetic nanoparticles (SMNP) and amino magnetic nanomicrosphere (AMNP) were synthesized successfully. The morphology, structure, magnetic property and chemical composition of the synthetic MNP and its derivatives were characterized using transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FT-IR) analysis, X-ray diffraction, superconducting quantum interference device (SQUID) and thermogravimetric analyses (TGA). All of these three nanoparticles exhibited good crystallization performance, apparent superparamagnetism, and the saturation magnetization of MNP, SMNP, AMNP were 47.9 emu/g, 33.0 emu/g and 19.5 emu/g, respectively. The amino content was 5.66%. The AMNP was used to immobilize lipase, and the maximum adsorption capacity of the protein was 26.3 mg/g. The maximum maintained activity (88 percent) was achieved while the amount of immobilized lipase was 23.7 mg $g^{-1}$. Immobilization of enzyme on the magnetic nanoparticles can facilitate the isolation of reaction products from reaction mixture and thus lowers the cost of enzyme application.

• Journal of Microbiology and Biotechnology
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• v.24 no.11
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• pp.1551-1558
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• 2014

The esterase gene Est8 from the thermophilic bacterium Bacillus sp. K91 was cloned and expressed in Escherichia coli. The monomeric enzyme exhibited a theoretical molecular mass of 24.5 kDa and an optimal activity around $50^{\circ}C$ at pH 9.0. A model of Est8 was constructed using a hypothetical YxiM precursor structure (2O14_A) from Bacillus subtilis as template. The structure showed an ${\alpha}/{\beta}$-hydrolase fold and indicated the presence of a typical catalytic triad consisting of Ser-11, Asp-182, and His-185, which were investigated by site-directed replacements coupled with kinetic characterization. Asp-182 and His-185 residues were more critical than the Ser-11 residue in the catalytic activity of Est8. A comparison of the amino acid sequence showed that Est8 could be grouped into the GDSL family and further classified as an SGNH hydrolase. Est8 is a new member of the SGNH hydrolase subfamily and may employ a different catalytic mechanism.

• Journal of Microbiology and Biotechnology
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• v.23 no.4
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• pp.437-443
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• 2013

The diversity of culturable non-actinobacterial (NA) bacteria associated with four species of South China Sea gorgonians was investigated using culture-dependent methods followed by analysis of the bacterial 16S rDNA sequence. A total of 76 bacterial isolates were recovered and identified, which belonged to 21 species of 7 genera, and Bacillus was the most diverse genus. Fifty-one percent of the 76 isolates displayed antibacterial activities, and most of them belonged to the Bacillus genus. From the culture broth of gorgonian-associated Bacillus methylotrophicus SCSGAB0092 isolated from gorgonian Melitodes squamata, 11 antimicrobial lipopeptides including seven surfactins and four iturins were obtained. These results imply that Bacillus strains associated with gorgonians play roles in coral defense mechanisms through producing antimicrobial substances. This study, for the first time, compares the diversity of culturable NA bacterial communities among four species of South China Sea gorgonians and investigates the secondary metabolites of gorgonian-associated B. methylotrophicus SCSGAB0092.

• Journal of Environmental Impact Assessment
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• v.19 no.4
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• pp.409-420
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• 2010

Heritage resources have become important social assets as the global interests in heritage tourism increased. Utilization of heritage as unique tourist destination has positive effects on regional economy, which in turn plays an important role in heritage conservation. However, it was found that the essential quality of heritage resources has been degraded and even destroyed when excessive use was permitted. This undesirable side-effects of heritage tourism have led to the growing concern for sustainable tourism that can contribute to both utilization and conservation of heritage. In order for sustainable tourism to be effectively implemented, it is crucial for visitors to be aware of the need for heritage conservation because they are the most important consumers of the heritage. The purpose of this study was to suggest some basic information for sustainable tourism by investigating visitors' evaluation of conservation and use values of heritage resources. The study sites were the two Korean traditional temples(Sokrisan Bubjusa and Naejangsan Naejangsa) and contingent valuation method was used. 390 visitors in Bubjusa and 308 visitors in Naejangsa participated in the questionnaire survey. From the results, sustainable tourism is possible in such heritage areas as traditional temples because the participants had relatively balanced values for heritage conservation and use. The WTP for natural conservation value was found to be highest in both temples; visitors may be more agreeable with the heritage conservation plan if the sustainability of natural resource is emphasized. Also, the use value of heritage could be influenced by the tourism utilization of the area where the heritage was located. If there are no other famous tourist destinations except the heritage in the area, additional plans to develop alternative destinations should be prepared in order to formulate the agreement on the need to protect the heritage among visitors, local communities, and government.

• Proceedings of the Korea Committee for Ocean Resources and Engineering Conference
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• 2001.05a
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• pp.83-86
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• 2001

This paper aims to make a feasibility study and to propose a master plan for the development of "integrated ocean energy utilization system", which utilizes various renewable ocean energies. Fossils energy, as the most important energy resources which are inevitable for the living and industrial development, becomes exhausted and its consumption creates serious environmental problem. It is important to utilize renewable ocean energy for a sustainable and environmentally friendly development. We survey the integrated utilization of ocean energy based on surveyed energy density distribution and propose concepts of integrated ocean energy utilization plant for both onshore and offshore system. The results of this study can support national effort for renewable energy development utilizing integrated ocean energy and refer as a guideline for the technical development of sustainable integrated ocean energy.

• Proceedings of the Korea Water Resources Association Conference
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• 2019.05a
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• pp.232-232
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• 2019

The persistence of drought periods and water scarcity is a growing public concern, as climate change projections indicate a more critical scenario in the future. The sustainability of water resources for the increasing population, and to ensuring crop production will unarguably be a daunting task for the water resources managers, with a projected 9.8 billion people by 2050 as well as the need to increase food production by 70 to 100%. Consequently, there is a need for significant irrigation water use for more crop production in the face of stiff competition among water users. However, the available natural resources are already over-constrained, and the allocation of more resources for food production is not feasible. Currently, about two-thirds of global water withdrawer is used by the agricultural sector while 48% of water resources in Korea is used for agricultural production. Despite the apparent ecological deficit and unfavorable conditions of resources utilization, a staggering amount of food waste occurs in the country. Moreover, wastage of food translates to waste of all the resources involved in the food production including water resources. Food waste can also be considered a serious potential for economic and environmental problems. Hence, exploring an alternative approach to efficient resources utilization in a more sustainable way can ensure considerable resources conservation. We hypothesized that reducing food waste will decline the demand for food production and consequently reduce the pressure on water resources. We investigated the food wastage across the food supply chain using the top-down datasets based on the FAO mass balance model. Furthermore, the water footprint of the estimated food wastage was assessed using the representative of selected food crops. The study revealed that the average annual food wastage across the food supply chain is 9.05 million tonnes, signifying 0.51 kg/capita/day and 48% of domestic food production. Similarly, an average of 6.29 Gm3 per annum of water resources was lost to food wastage, which translates to 40% of the total allotted water resources for agriculture in the country. These considerable resources could have been conserved or efficiently used for other purposes. This study demonstrated that zero food waste generation would significantly reduce the impact on freshwater resources and ensure its conservation. There is a need for further investigation on the food waste study using the bottom-up approach, specifically at the consumer food waste, since the top-down approach is based on estimations and many assumptions were made.

• Journal of Energy Engineering
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• v.29 no.3
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• pp.57-63
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• 2020

In this paper, we reported that the global trend of cement production and utilization as raw materials and as a fuel. As we know, cement is one of the significant materials required for the construction industry. The recent trend of rising urbanization, both the cement and construction industry played a vital role. The cement industry is a major sustainable infrastructure for the countries. Currently, China producing cement half of the world's cement production. During the year 2018, Korea producing cements nearly 57.5 million metric tons. Waste materials are used as circular resources and also having tremendous benefits for cement production. Another important use of these circular resources is fuel for the cement industry. There is a large potential benefit of the cement industry, but it's creating a severe environmental threat. The cement industry contributes to the major emissions of CO2. This leads the global warming. As per the Paris agreement, the Korean government initiated the recycling policy of waste materials and also the utilization of circular resources for the prevention of limited natural resources and also the global warming effect.

• Proceedings of the Korea Water Resources Association Conference
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• 2019.05a
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• pp.408-408
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• 2019

The persistence of drought periods and water scarcity is a growing public concern, as climate change projections indicate a more critical scenario in the future. The sustainability of water resources for the increasing population, and to ensuring crop production will unarguably be a daunting task for the water resources managers, with a projected 9.8 billion people by 2050 as well as the need to increase food production by 70 to 100%. Consequently, there is a need for significant irrigation water use for more crop production in the face of stiff competition among water users. However, the available natural resources are already over-constrained, and the allocation of more resources for food production is not feasible. Currently, about two-thirds of global water withdrawer is used by the agricultural sector while 48% of water resources in Korea is used for agricultural production. Despite the apparent ecological deficit and unfavorable conditions of resources utilization, a staggering amount of food waste occurs in the country. Moreover, wastage of food translates to waste of all the resources involved in the food production including water resources. Food waste can also be considered a serious potential for economic and environmental problems. Hence, exploring an alternative approach to efficient resources utilization in a more sustainable way can ensure considerable resources conservation. We hypothesized that reducing food waste will decline the demand for food production and consequently reduce the pressure on water resources. We investigated the food wastage across the food supply chain using the top-down datasets based on the FAO mass balance model. Furthermore, the water footprint of the estimated food wastage was assessed using the representative of selected food crops. The study revealed that the average annual food wastage across the food supply chain is 9.05 million tonnes, signifying 0.51 kg/capita/day and 48% of domestic food production. Similarly, an average of $6.29Gm^3$ per annum of water resources was lost to food wastage, which translates to 40% of the total allotted water resources for agriculture in the country. These considerable resources could have been conserved or efficiently used for other purposes. This study demonstrated that zero food waste generation would significantly reduce the impact on freshwater resources and ensure its conservation. There is a need for further investigation on the food waste study using the bottom-up approach, specifically at the consumer food waste, since the top-down approach is based on estimations and many assumptions were made.

• Proceedings of the Korea Water Resources Association Conference
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• 2019.05a
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• pp.161-161
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• 2019

Water security issues, stimulated by increasing population and changing climate, are growing and pausing major challenges for water resources managers around the world. Proper utilization, management and distribution of all available water resources is key to sustainable development for achieving water security To alleviate the water shortage, most of the current research on multi-sources combined water supplies depends on an overall generalization of regional water supply systems, which are seldom broken down into the detail required to address specific research objectives. This paper proposes the concept of optimization framework on multi water sources selection. A multi-objective water allocation model with four objective functions is introduced in this paper. Harmony search algorithm is employed to solve the applied model. The objective functions addresses the economic, environmental, and social factors that must be considered for achieving a sustainable water allocation to solve the issue of water security.

• Ocean and Polar Research
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• v.33 no.4
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• pp.497-506
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• 2011

The rapid industrial and technological development has made the human activities for the utilization of marine resources more complex. Marine spatial management is a space-based approach. It is a comprehensive and integrated management approach. The ultimate goal of marine spatial management is the "sustainable use" of marine resources. The partial approach is applied in the existing marine spatial management, mainly coastal zones which involves integrated approach. Also this showed various limitations including restricted mostly to coastal zones, and limitation to implementation tools. However, for marine spatial management to have a reasonable approach that attaches importance to the relationship between humans and the holistic ecosystem, it is important to internalize a central principle in marine spatial management that focuses on the sustainable use of marine resources. In the present study, four central principles are proposed that will eventually be applied through marine spatial management planning tools. These principles are 1) the establishment of a cooperative decision making and planning system that is based on stakeholder participation; 2) scientific assessment of the current status and impact on the basis of ecology, sociology, and economics; 3) reasonable and optimal spatial assignment based on the forecasting of future-use characteristics and environmental changes; and 4) ascribing importance to the implementation of the results of rational planning processes.