• BMB Reports
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• v.32 no.5
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• pp.451-455
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• 1999

The concentrations of L-ascorbic acid (AsA, ascorbate, vitamin C) and its biosynthetic and metabolically-related enzymes such as L-galactono-1,4-lactone dehydrogenase (GLDase), ascorbate peroxidase (APX), and ascorbate oxidase (ASO) were investigated in suspension cultures of Scutellaria baicalensis. Cells growing from 4 days after subculture (DAS) to 9 DAS and from 16 DAS to 19 DAS showed a diauxic growth, and then growth rapidly decreased with further culturing. The AsA content slowly increased to 19 DAS, reached a maximum at 21 DAS (ca $120\;{\mu}g/g$ dry cell wt), and then rapidly decreased with further culturing. GLDase and ASO activity were well correlated with the cell growth curve, showing a maximum at 19 DAS, whereas APX activity showed a good correlation with the changes in AsA content, showing a maximum at 21 DAS. The total ascorbate contents (reduced form, AsA, and oxidized form, dehydroascorbate, DHA) were markedly enhanced at 10 DAS when L-galactose and L-galactono-1,4-lactone (25 mM) were added to SH medium supplemented with 20 g/l sucrose at 9 DAS, by 5.5 and 6.8 times, respectively. DHA composed more than 90% of the total ascorbate contents in suspension cultures of S. baicalensis, even though the ratio of reduced to oxidized form slightly varied with cell growth stage. The results indicate that L-galactose and L-galactono-1,4-lactone are effective precursors of AsA in cell cultures of S. baicalensis, and that in vitro cultured cells provide suitable biomaterials for the study of biosynthesis and metabolism of AsA.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.21 no.6
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• pp.555-561
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• 2008

This paper describes an e-Engineering framework to support collaborative design of automotive suspension systems developed at KIMM(Korea Institute of Machinery and Materials). The e-Engineering framework is proposed and developed on the base of the multi-layered software agents including engineering task agent which is generated from the domain knowledge of experts. The developed framework is aim to widely spread application to the small and medium enterprises by adopting open source technologies such as JADE (Java Agent Development Framework) and by using the independent characteristics related with applicant H/W and 81W system. This framework can provide an integrated design environment to support distributed personnel, design activities and engineering resources during product development process. For the validation of the system's applicability and efficiency, the several practical design processes for automotive suspension systems of RR/FR lower arms and RR cross member are applied and discussed.

• Journal of Plant Biology
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• v.31 no.2
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• pp.91-100
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• 1988

The effect of Ca2+ and polyamines on the activity $\beta$-glucan synthetase II(GSII) related to cell wall synthesis was studied in carrot suspension cultured cells. The activity of GS II is four times higher than that of $\beta$-glucan synthetase I in carrot suspension cultured cells and in vitro expreiment, the activity of GSII was increased in response to increase in concentration of Ca2+ and polyamines. When carrot suspension cultured cells were incubated together with Ca2+ and polyamines, the GSII activity was high at 0.1mM of Ca2+ and 1mM of putrescine. Also, polycationic poly-L-lysine and poly-L-ornithine increased about 50% the GSII activity than that of the control, respectively. These results may imply that Ca2+ and polyamines were related to the enzyme activity as a polycationic nature. In addition, verapamil as the calcium channel blocker and flunarizine as an antagonist of calcium mechanism in cytoplasm decreased GSII activity ramarkably, Ca2+ and calmodulin stimulated GSII activity as Ca2+ of free ion rather than Ca2+ calmodulin complex. The effect of 2,4-D on the GSII activity in culture medium is shown to be low at 0.1mg per liter and GSII activity increased about 30% more than that of the 0.1mg/l at the range of 0.3-1.0mg per litere. Cummulative results suggest that Ca2+ and polyfamines stimulate the cell wall synthesis by means of the enhancement of GSII activity responsible for synthesizing the cell wall components.

• International journal of steel structures
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• v.18 no.4
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• pp.1252-1264
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• 2018

Long span bridges such as steel cable stayed and suspension bridges are usually more flexible than short to medium span bridges and expected to have large deformations. Deflections due to live load for long span bridges are important since it controls the overall heights of the bridge for securing the clearance under the bridge and serviceability for securing the comfort of passengers or pedestrians. In case of sea-crossing bridges, the clearance of bridges is determined considering the height of the ship master from the surface of the water, the trim of the ship, the psychological free space, the tide height, and live load deflection. In the design of bridges, live load deflection is limited to a certain value to minimize the vibrations. However, there are not much studies that consider the live load deflection and its effects for long span bridges. The purpose of this study is to investigate the suitability of live load deflection limit and its actual effects on serviceability of bridges for steel cable-stayed and suspension bridges. Analytical study is performed to calculate the natural frequencies and deflections by design live load. Results are compared with various design limits and related studies by Barker et al. (2011) and Saadeghvaziri et al. (2012). Two long span bridges are selected for the case study, Yi Sun-Sin grand bridge (suspension bridge, main span length = 1545 m) and Young-Hung grand bridge (cable stayed bridge, main span length = 240 m). Long-term measured deflection data by GNSS system are collected from Yi Sun-Sin grand bridge and compared with the theoretical values. Probability of exceedance against various deflection limits are calculated from probability distribution of 10-min maximum deflection. The results of the study on the limitation of live load deflection are expected to be useful reference for the design, the proper planning and deflection review of the long span bridges around the world.

• Journal of Life Science
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• v.14 no.6 s.67
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• pp.1018-1022
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• 2004

Optimized conditions for Agrobacterium-mediated lisianthus pollen transformation were adjusted using various factors such as temperature, pH and sucrose concentration. Pollen tube growth was successfully achieved in a medium (pollen germination medium; PGM) containing $7-15\%$ sucrose with pH in the range of 5.5-7.0 at temperature of $20-27^{\circ}C$. Lisianthus pollen was vacuum-infiltrated with Agrobacterium cell suspension for 20 min, and transformed pollen was confirmed by GUS histochemistry and Southern hybridization following RT-PCR. Transgenic pollen system may be utilized for establishing an area of plant transient expression systems based on the convenient pollen transformation procedure presented in here.

• Applied Chemistry for Engineering
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• v.19 no.6
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• pp.609-616
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• 2008

The present study is concerned with a simple numerical method for estimating the hindered settling velocity of noncolloidal suspensions with bidisperse size distribution of particles. The method is based on an effective-medium theory which uses the conditional ensemble averages for describing the velocity fields or other physical quantities of interest in the suspension system with the particles randomly placed. The effective-medium theory originally developed by Acrivos and Chang[1] for monodisperse suspensions is modified for the bidisperse case. Using the radial distribution functions and stream functions the hindered settling velocity of the suspended particles is calculated numerically. The predictions by the present method are compared with the previous experimental results by Davis and Birdsell[2] and Cheung et al.[3]. It is shown that the estimations by the effective-medium model of the present study reasonably agree with the experimental results.

• Korean Journal of Plant Tissue Culture
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• v.27 no.2
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• pp.149-154
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• 2000

Embryogenic suspension cultures were initiated using embryogenic callus from immature inflorescence explants (Aralia cordata Thunb.) cultured on solid MS medium containing 1 mg/L 2,4-D for 8 weeks and then the embryogenic callus was proliferated in liquid MS medium containing 1 mg/L 2,4-D. After sieving the suspensions (pore size 270$\mu$m), embryogenic cells were cultured in liquid MS medium with cytokinins (kinetin, BA, zeatin) for two weeks. When the embryogenic cells were transferred to liquid MS basal medium, primary somatic embryos were developed after 5 weeks of culture. Secondary embryos were developed directly from the primary torpedo and cotyledonary embryos cultured in solid MS basal medium. Frequency of secondary embryogenesis was higher on medium containing 2 mg/L kinetin than the other cytokinins. Plant regeneration was highly recorded by placing secondary cotyledonary embryos induced from primary cotyledonary embryos in MS medium containing 2 mg/L kinetin or 2 mg/L zeatin (25.4% and 28.6%, respectively). The plant regeneration from secordary embryos was prohibited by tertiary embryogenesis.

• FLOWER RESEARCH JOURNAL
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• v.17 no.2
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• pp.114-120
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• 2009

The most effective conditions of in vitro culture were studied for mass propagation of Pteris cretica 'Wilsonii'. Spores of the species germinated within 7 weeks. The greatest proliferation was obtained with Knop and Hyponex media, but growth was more effective in Hyponex medium. MS medium induced necrosis of prothalli in all strength of nitrogen and sucrose except in case of 0% sucrose. Hyponex medium supplemented with 1% sucrose and 0.6% agar promoted propagation and growth of prothalli. In Hyponex medium, optimal inoculation method was homogenization, but in MS medium dividing colonies of prothalli was more effective. Culturing on solid medium was more effective than liquid culture method. Liquid culture induced necrosis of prothalli. Shaking cultured prothalli showed good growth, but propagation was inhibited compared to those cultured on solid medium.

• Korea-Australia Rheology Journal
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• v.13 no.1
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• pp.19-27
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• 2001

In this symposium paper, the migration and hydrodynamic diffusion of non-colloidal, spherical particles suspended in polymer solutions are considered under Poiseuille or torsional flows. The migration phenomena in polymer solutions are compared with those in Newtonian fluids and the effect of fluid elasticity is discussed. The experimental results on particle migration in dilute polymer solution reveal that even a slight change in the rheological property of the dispersing medium can induce drastic differences in flow behavior and migration of particles, especially in dilute and semi-concentrated suspensions.

• Journal of the Korean Ceramic Society
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• v.28 no.3
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• pp.205-214
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• 1991

Calcium carbonate fine powders were synthesized by blowing CO2 gas in CaO or Ca(OH)2 suspension, and the shapes of powders obtained were examined for each synthetic condition. When water was used as a solvent, ultrafine calcite powders with the average size of∼0.03$\mu\textrm{m}$ were obtained. When synthesized using methanol as a solvent, amorphous phase and spherical vaterite phase were obtained by suction filtering and non-filtering, respectively. Reaction did not occured in ethanol medium, but spherical vaterite phase was obtained by adding ethylene glycol in ethanol.