• Applied Biological Chemistry
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• v.19 no.2
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• pp.82-92
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• 1976

The study was carried out to investigate the changes of the various chemical components and the microflora during the aging period of Korean navive Kochuzang. (Red pepper soybean paste) Korean native maeju loaves were separated into surface and inner parts. Three kinds of Korean native Kochuzang were prepared from surface part, inner part, and ordinary of maeju. The selection and the indentification of the high enzyme producing strains from the microflora and characteristics of their enzymes were studied. I. The changes of the various chemical components during the aging period of Kochuzang. 1) The changes of pH in the 3 kinds of Kochuzang displayed rapid decrease for the first 10 days after preparing and gradual curve of decrease until 60 days, but slight increase for the next 30 days. The pH of the surface part Kochuzang was lower than that of inner part or ordinary Kochuzang. 2) The total acid contents in the 3 kinds of Kochuzang showed gradual increase until the 60 days but it slowly reduced after this time. 3) The total nitrogen contents in the 3 kind of Kochuzang showed gradual inerease up to the 60 days, but slight decrease after this time. 4) The changes of trichloroacetic acid soluble nitrogen in the 3 kinds of Kochuzang showed a remarkable increase for the first 10 days, however gradual increase after this time. 5) The increase of amino nitrogen contents in the 3 kinds of Kochuzang seemed to be remarkable until the first 30 days, however to be less remarkable after this time. 6) The contents of reducing sugar in the 3 kinds of Kochuzang showed remarkable increase until the first 50 days and it slowly reduced after this time. II. The changes of microflora during the aging period of Kochuzang. 1) Aerobic, anaerobic bacteria and mold in the 3 kinds of Kochuzang were increased until the first 30 to 40 days, but they were reduced after this time. 2) No yeast in the three kinds of Kochuzang appeared until the first 20 days. Yeast were proved to grow, when the pH value was decreased below 5.4 after the 30 days. Yeasts in the surface part and ordinary Kochuzang were gradually increased and those in the inner part Kochuzang were decreased as aging. III. The selection and identification of high amylase and protease producing strains from the microflora during the aging period of Kochuzang. 1) The amylase and protease highly producing strains from microflora were identified as Bacillus subtilis-P, Bacillus subtilis-G, Bacillus licheniformis-K, Aspergillus oryzae-B. 2) Amylase activity of Aspergillus oryzae-B was highest among the strains and the strains in order of the higher activity to the lower one were Bacillus subtilis-P Bacillus licheniformis-K, Bacillus subtilis-G. Protease activities of Aspergillus oryzae-B and Bacillus subtilis-P were about the same and the strains in order of the higher activity to the lower one were Bacillus licheniformis-K, Bacillus subtilis-G. 3) Amylase activity was inhibited more than protease activity was with NaCl concentration. Amylase activity was inhibited by 45 to 65 percent and protease activity by 40 to 46 percent at the concentration of 15 percent NaCl, which was the average concentration of NaCl in Kochuzang.

• Radiation Oncology Journal
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• v.24 no.4
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• pp.300-308
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• 2006

$\underline{Purpose}$: To develop a wireless CCTV system in semi-beam's eye view (BEV) to monitor daily patient setup in radiation therapy. $\underline{Materials\;and\;Methods}$: In order to get patient images in semi-BEV, CCTV cameras are installed in a custom-made acrylic applicator below the treatment head of a linear accelerator. The images from the cameras are transmitted via radio frequency signal (${\sim}2.4\;GHz$ and 10 mW RF output). An expected problem with this system is radio frequency interference, which is solved utilizing RF shielding with Cu foils and median filtering software. The images are analyzed by our custom-made software. In the software, three anatomical landmarks in the patient surface are indicated by a user, then automatically the 3 dimensional structures are obtained and registered by utilizing a localization procedure consisting mainly of stereo matching algorithm and Gauss-Newton optimization. This algorithm is applied to phantom images to investigate the setup accuracy. Respiratory gating system is also researched with real-time image processing. A line-laser marker projected on a patient's surface is extracted by binary image processing and the breath pattern is calculated and displayed in real-time. $\underline{Results}$: More than 80% of the camera noises from the linear accelerator are eliminated by wrapping the camera with copper foils. The accuracy of the localization procedure is found to be on the order of $1.5{\pm}0.7\;mm$ with a point phantom and sub-millimeters and degrees with a custom-made head/neck phantom. With line-laser marker, real-time respiratory monitoring is possible in the delay time of ${\sim}0.17\;sec$. $\underline{Conclusion}$: The wireless CCTV camera system is the novel tool which can monitor daily patient setups. The feasibility of respiratory gating system with the wireless CCTV is hopeful.

• The Korean Journal of Malacology
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• v.24 no.1
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• pp.41-50
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• 2008

The histochemical characteristics and ultrastructure of the mantle in the spiny top shell, Batillus cornutus were described using light and electron microscopy. The simple epidermal layer wrapped on the top and bottom of the centrally located connective tissue. And then the epidermal layer were divided into the outer epidermal layer near a shell and the inner epidermal layer closed to the visceral mass. The connective tissue layer was composed of the collagen fiber muscularfiber bundle and hemolymph sinus. Mucous cells in the apical mantle contained acid and neutral mucopolysaccaride, and acidic carboxylated mucopolysaccaride in the mid and marginal mantle. The mantle thickness, epidermal layer thickness and hemolymph sinus area displayed a trend of reduction from the marginal zone to the apical zone. From TEM observation, it was possible to distinguish epithelium, ciliated cell, absorptive cell and secretory cell in the epidermal layer. The epithelia were columnar and the nucleus was elliptical. The free surface were covered with microvilli. The lateral membranes of epithelium was con nected with neighboring cells by the zonular occludens, zonular adherens and membrane interdigitation. Ciliated cell on free surface had cilia and microvilli, and numerous mitochondria in the apical cytoplasm. In the epidermal layer, it observed 2 type cells having absorptive function. The absorptive cells were columnar in shape, and contained microvilli, pinocytotic vesicles, mitochondria and lysosomes of various electron density. Secretory cells can be divided into four types (A, B, C, D) depending on the cell shape and characteristics of secretory granules. These cells were unicellular glands and had similar characteristics to previously reported on the mantle of the gastropod and bivalves.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.13 no.3
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• pp.200-209
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• 2008

Particle fluxes were measured with a time-series sediment trap from July 2003 to June 2005 at the St. KOMO(KOMO; Korea Deep-Sea Environmental Study Long-Term Monitoring Station, $10^{\circ}30'N,\;131^{\circ}20'W$) in the northeastern Pacific. Total mass fluxes at a depth of 4,960 m showed distinct seasonal variations with high values in the winter(December-February) and spring(March-May) and low values in the summer(June-August) and fall(September-November). Biogenic origin fluxes also displayed distinct seasonal variations similar to total mass fluxes. Particularly, calcium carbonate fluxes in winter and spring were more than two times greater than those in summer and fall. The prominent seasonal variations of total mass and biogenic fluxes were closely related with the seasonal changes of primary production in the surface waters; in winter and spring, primary production increased due to the enhanced supply of nutrients below the surface mixed layer by strong wind and less stratification, whereas it decreased as a result of the less supply of nutrient by reduced wind speed and strong stratification in summer and fall. The seasonal variations of total mass and biogenic fluxes in this study were higher than the differences of total mass and biogenic fluxes caused by the environmental changes such as El $Ni\tilde{n}o$ and La $Ni\tilde{n}a$ events in the previous studies. In order to understand the effects of El $Ni\tilde{n}o$ and La $Ni\tilde{n}a$ on the particle flux, therefore, the seasonal variation of particle flux in the northeastern equatorial Pacific needs to be well defined.

• Korean Journal of Remote Sensing
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• v.34 no.2_1
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• pp.237-248
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• 2018

The vertical distribution of hydrometeor before precipitation near the cloud base has been analyzed using a scanning lidar, rawinsonde data, and Cloud-Resolving Storm Simulator (CReSS). This study mostly focuses on 13 Desember 2016 only. The typical synoptic pattern of lake-effect snowstorm induced easterly in the Yeongdong region. Clouds generated due to high temperature difference between 850 hPa and sea surface (SST) penentrated in the Yeongdong region along with northerly and northeasterly, which eventually resulted precipitation. The cloud base height before the precipitation changed from 750 m to 1,280 m, which was in agreement with that from ceilometer at Sokcho. However, ceilometer tended to detect the cloud base 50 m ~ 100 m below strong signal of lidar backscattering coefficient. As a result, the depolarization ratio increased vertically while the backscattering coefficient decreased about 1,010 m~1,200 m above the ground. Lidar signal might be interpreted to be attenuated with the penetration depth of the cloud layer with of nonspherical hydrometeor (snow, ice cloud). An increase in backscattering signal and a decrease in depolarization ratio occured in the layer of 800 to 1,010 m, probably being associated with an increase in non-spherical particles. There seemed to be a shallow liquid layer with a low depolarization ratio (<0.1) in the layer of 850~900 m. As the altitude increases in the 680 m~850 m, the backscattering coefficient and depolarization ratio increase at the same time. In this range of height, the maximum value (0.6) is displayed. Such a result can be inferred that the nonspherical hydrometeor are distributed by a low density. At this time, the depolarization ratio and the backscattering coefficient did not increase under observed melting layer of 680 m. The lidar has a disadvantage that it is difficult for its beam to penetrate deep into clouds due to attenuation problem. However it is promising to distinguish hydrometeor morphology by utilizing the depolarization ratio and the backscattering coefficient, since its vertical high resolution (2.5 m) enable us to analyze detailed cloud microphysics. It would contribute to understanding cloud microphysics of cold clouds and snowfall when remote sensings including lidar, radar, and in-situ measurements could be timely utilized altogether.

• Horticultural Science & Technology
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• v.28 no.6
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• pp.996-1002
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• 2010

Flow cytometry (FCM) was used to measure the ploidy level of three different sports from 'Campbell Early' ($Vitis$ $labruscana$) grape. Results of the study showed different ploidy levels. FCM analysis for 'Campbell Early' grape which contains 2C DNA diploid cells showed single peak around 35-40 while 'Kyoho' grape with 4C DNA tetraploid cells had a different level of 70-80. However, analysis of the sports displayed a histogram with 2 peaks containing both 2C and 4C nuclei. There was no difference in histograms of 2C DNA flesh and pericarp; on the other hand, 4C DNA flesh type of sports had a different histogram from that of the 2C DNA pericarp. Chromosome numbers of diploid ('Campbell Early'), tetraploid ('Kyoho'), and three sports were counted under the microscope. 'Campbell Early' and 'Kyoho' have 38 and 76 chromosomes, respectively. Three different sports are mixoploids with mixtures of diploid and tetraploid cells. Microscopic observations of shoot apical meristems in sports from 'Campbell Early' grape were carried out to determine the type of plant chimera. 'Campbell Early' grape (diploid) and 'Kyoho' grape (tetraploid) showed that both had 2 tunica layers covering corpus cells, while the three different sports had tunica layers showing mostly oblique division. Most cells from 'Kyoho' grape were larger than 'Campbell Early' grape. Cells from L-2 and L-3 layers of the three sports were similar to 'Kyoho' grape in size, although all cells in L-1 surface layer were uniform in size like 'Campbell Early' grape. Results of FCM analysis indicated that both normal and polyploid cells could be intermixed in sports and could become mixoploidy consisting of diploid and tetraploid. All sports used in the tests were periclinal chimera plants with two distinct L-1 and L-2 cell layers. The result of this study suggests that all three sports which originated from 'Campbell Early' grape might be 2-4-4 type chimera formation.

• Journal of Energy Engineering
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• v.25 no.4
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• pp.103-109
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• 2016

Polyethylene pipes has useful benefits which are anti-corrosive and flexible material, so it is used to gas pipes but also class 3 water pipes of nuclear power plant, process pipes of petrochemical plant and chemical plant. So the usage of polyethylene pipes is widely increased. But it has been limited for the usage of polyethylene, because it can not be directly detected to fusion joints by using non destructive evaluation. Polyethylene pipes are connected by two methods, one is butt fusion and the other is electrofusion. Butt fusion is widely used to connecting the pipes. It is proposed to method for determining the reliability of joints in this study that is detection of the melt flow zone at fusion joints. In this study, middle density polyethylene is used, outside diameter of the test specimen is 225mm and thickness is 20.5mm. Speed of ultrasonic of this test specimen is 2,200m/s. Test specimens were fabricated by varying the heating time which means from 0% to 130% applying time through heating plate to polyethylene for detecting melt flow zone. Also 4 additional test specimens were made, one was made that not scrapping attached surface of pipes but applying 100% of the proper heating time and the others were made to include of soil, gravel and vinly tape paper at fusion joints, that were also applied 100% of proper heating time. Ultrasonic testing to measure the melt flow zone of 20 test specimens was conducted by using 3.5MHz and 5.0MHz ultrasonic probes and melt flow zone measuring was conducted to three times at different point to one specimen. To differentiate the melt flow zone signal, post image processing was equally conducted to all test results and image levels, contrast, sharpen, threshold were adopted to all teat results and the test results were displayed gray scale. From the results, for the shorter heating times the reflection area of multiple echo have been increased, so the data was obtained from the position where it can be eliminated as much as possible. At 80% of proper heating time(168 sec.), the signal of melt flow zone was obtained clearly, so measuring could be conducted. From 7% of proper heating time(15 sec.) to shorter heating times. we could not obtain the signal because test specimen was not fused. From the result, we can verify that measuring of melt flow zone by using phased array ultrasonic imaging method is possible. And we can verify to complete and incomplete butt fusion by measuring the melt flow zone.

• Journal of Dental Rehabilitation and Applied Science
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• v.24 no.4
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• pp.337-349
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• 2008

Despite an improved bone reactions of Mg-incorporated implants in the animals, little yet has been carried out by the experimental investigations in functional loading conditions. This study investigated the clinical and histologic parameters of osseointegrated Mg-incorporated implants in delayed loading conditions. A total of 36 solid screw implants (diameter 3.75 mm, length 10mm) were placed in the mandibles of 6 beagle dogs. Test groups included 18 Mg-incorporated implants. Turned titanium Implants served as control. Gold crowns were inserted 3 months. Radiographic assessments and stabilitytests were performed at the time of fixture installation, $2^{nd}$ stage surgery, 1 and 3 months after loading. Histological observations and morphometrical measurements were also performed. Of 36 implants, 32 displayed no discernible mobility, corresponding to successful clinical function. There was no statistically significant difference between test implants and controls in marginal bone levels (p=0.413) and RFA values. The mean BIC % in the Mg-implants was $54.4{\pm}20.2%$. The mean BIC % in the turned implant was $48.9{\pm}8.0%$. These differences between the Mg-implant and control implant were not statistically significant (P=0.264). In the limitation of this study, bone-to-implant contact (BIC) and bone area of Mg-incorporated oxidized implant were similar to machine-turned implant. The stability analysis showed no significantly different ISQ values and marginal bone loss between two groups. Considering time-dependent bone responses of Mg-implant, it seems that Mg-implants enhanced bone responses in early loading conditions and osseointegrated similarly to cp Ti implants in delayed loading conditions. However, further investigations are necessary to obtain long-term bone response of the Mg-implant in human.

• Journal of Dental Rehabilitation and Applied Science
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• v.36 no.3
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• pp.183-195
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• 2020

Purpose: The purpose of this study is to compare the flexural strength of CAD/CAM denture base resins with conventional denture base resins based on their thicknesses. Materials and Methods: For the conventional denture base resins, Lucitone 199^® (C-LC) was used. DIOnavi - Denture (P-DO) and DENTCA Denture Base II (P-DC) were taken for the 3D printing denture base resins. For the prepolymerized PMMA resins, Vipi Block Gum (M-VP) and M-IVoBase^® CAD (M-IV) were used. The final dimensions of the specimens were 65.0 mm x 12.7 mm x 1.6 mm / 2.0 mm / 2.5 mm. The 3-point bend test was implemented to measure the flexural strength and flexural modulus. Microscopic evaluation of surface of fractured specimen was conducted by using a scanning electron microscope (SEM). After testing the normality of the data, one-way ANOVA was adopted to evaluate the differences among sample groups with a significance level of P = 0.05. The Tukey HSD test was performed for post hoc analysis. Results: Under the same thicknesses, there are significant differences in flexural strength between CAD/CAM denture base resins and conventional denture base resins except for P-DO and C-LC. M-VP showed higher flexural strength than conventional denture base resins, P-DC and M-IV displayed lower flexural strength than conventional denture base resins. Flexural modulus was highest in M-VP, followed by C-LC, P-DO, P-DC, M-IV, significant differences were found between all materials. In the comparison of flexural strength according to thickness, flexural strength of 2.5 mm was significantly higher than that of 1.6 mm in C-LC. Flexural strength of 2.5 mm and 2.0 mm was significantly higher than that of 1.6 mm in P-DC and M-VP. In M-IV, as the thickness increases, significant increase in flexural strength appeared. SEM analysis illustrates different fracture surfaces of the specimens. Conclusion: The flexural strength of different CAD/CAM denture base resins used in this study varied according to the composition and properties of each material. The flexural strength of CAD/CAM denture base resins was higher than the standard suggested by ISO 20795-1:2013 at a thickness of 1.6 mm or more though the thickness decreased. However, for clinical use of dentures with lower thickness, further researches should be done regarding other properties at lower thickness of denture base resins.

• Food Science and Preservation
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• v.17 no.5
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• pp.659-666
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• 2010

A central composite design was used to investigate the effects of the three independent variables of extraction temperature ($X_1$), ethanol concentration ($X_2$), and extraction time ($X_3$), on dependent variables including yield ($Y_1$), total phenol levels ($Y_2$), electron-donating ability ($Y_3$), brownness ($Y_4$), and reducing sugar content ($Y_5$) of Vitis Coignetiae. Yield was affected by extraction temperature and time. The maximum yield was obtained at $91.62^{\circ}C(X_1)$, and, 25.37% (w/v) ethanol ($X_2$), after 317.70 min of extraction ($X_3$), evident as a saddle when displayed graphically. Total phenol levels were essentially unaffected by extraction temperature or ethanol concentration, but were highly influenced by extraction time. The maximum total phenol levels was 4,763.46 GAE mg/100 g obtained at $88.20^{\circ}C(X_1)$, and 47.79% (w/v) ethanol ($X_2$), after 349.32 min ($X_3$) of extraction. Electron-donating ability (EDA) was affected by extraction temperature and time. Maximum EDA was 55.90% at $86.72^{\circ}C(X_1)$, 50.61% (w/v) ethanol ($X_2$), and 265.96 min ($X_3$) of extration time, again shown by a graphical saddle. Brownness was affected by extraction time. The maximum extent of brown coloration was obtained at $82.66^{\circ}C(X_1)$, 99.27% (w/v) ethanol ($X_2$), and 252.63 min of extraction time ($X_3$), once again shown by graphical saddle. The maximum reducing sugar content was obtained at $96.24^{\circ}C(X_1)$, 22.59% (w/v) ethanol ($X_2$), and 216.06 min extraction time($X_3$).