• Journal of Sensor Science and Technology
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• v.17 no.5
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• pp.369-374
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• 2008

A novel fiber optic surface plasmon resonance (SPR) sensor using cyclic olefin copolymer (COC) prism with the spectral modulation is presented. The SPR sensor chip is fabricated using the SU-8 photolithography, Ni-electroplating and COC injection molding process. The sidewall of the COC prism is partially deposited with Au/Cr (45/2.nm thickness) by e-beam evaporator, and the thermal bonding process is conducted for micro fluidic channels and optical fibers alignment. The SPR spectrum for a phosphate buffered saline (0.1.M PBS, pH.7.2) solution shows a distinctive dip at 1300.nm wavelength, which shifts toward longer wavelength with respect to the bovine serum albumin (BSA)concentrations. The sensitivity of the wavelength shift is $1.16\;nm{\cdot}{\mu}g^{-1}{\cdot}{\mu}l^{-1}$. From the wavelength of SPR dips, the refractive indices (RI) of the BSA solutions can be theoretically calculated using Kretchmann configuration, and the change rate of the RI was found to be $2.3{\times}10^{-5}RI{\cdot}{\mu}g^{-1}{\cdot}l^{-1}$. The realized fiber optic SPR sensor with a COC prism has clearly shown the feasibility of a new disposable, low cost and miniaturized SPR biosensor for biochemical molecular analyses.

• Journal of Sensor Science and Technology
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• v.16 no.6
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• pp.401-406
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• 2007

In this paper, we fabricated the polymer waveguide type surface plasmon resonance (SPR) sensor using a white light source and optical spectrum analyzer (OSA). Fabricated sensor uses the principle of phase matching between evanescent wave and surface plasmon wave. According to the measuring result, the shift of resonance wavelength conducts the change of the refractive index. The proposed SPR sensor is expected to apply the integrated multichannel SPR sensor and the realtime monitoring system.

• BMB Reports
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• v.31 no.1
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• pp.101-105
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• 1998

Immunoassay of botulinum toxin (BTX) B type was investigated using two typed of biosensors: light addressable potentiometric sensor (LAPS) and surface plasmon resonance (SPR) sensor. Urease-tagged and immuno-filtration capture method have been used for LAPS. Tag-free and direct binding real-time detection method have been used for SPR sensor. The detection limit of sandwich assay format with LAPS was 10 ng/ml, which was the lowest among methods tested. SPR has the advantage of being more convenient because tag-free direct binding assay can be used and reaction time was reduced, regardless of low sensitivity. This result shows that sandwich assay format with LAPS can be used as an alternative method of BTX mouse bioassay which is known as the most sensitive method for the detection of BTX.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.56 no.1
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• pp.156-160
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• 2007

Surface plasmon resonance(SPR) has been widely studied for biological and chemical sensing applications. The present study conducts numerical simulation for the single and bimetallic layer SPR configurations by using the multiple beam interference matrix(MBIM) method to investigate the influence of wave interference and complex refractive indices of materials on optical characteristics such as reflectance and optical phase shift which are used for sensing. First, calculated reflectances are compared with experimental data for validation. In addition, in the single film structures this study finds out the appropriate film thicknesses with minimum reflectance for cases of gold film and silver film. For a bimetallic silver-gold film structure, in particular, the bimetallic film thicknesses that has the minimum reflectance are found 36 nm for silver and 5 nm for gold. From the results, the use of phase shift would be useful compared to reflectance in determining the SPR configuration because the phase shift becomes more sensitive than reflectance.

• Journal of Sensor Science and Technology
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• v.26 no.6
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• pp.402-407
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• 2017

Characteristics of various material surface plasmon resonance (SPR) chips were investigated in angular interrogation mode and intensity interrogation mode. Among five metals, silver (Ag), gold (Au), copper (Cu), chromium (Cr) and titanium (Ti), three metals, Ag, Au and Cu were paid attention to since their characteristics can be easily analyzed in angular interrogation mode by investigating the change of their reflectance curves according to refractive index change from 1.331 to 1.335. Most of SPR chips with various configurations showed the similar property in angular interrogation mode. The application of the SPR chip made of Ag, Au and Cu or their combinations depends on their reflectance properties. In intensity interrogation mode, the operation range may be limited since the variation of the intensity was not linearly related to refractive index change ranging from 1.331 to 1.335. However, the SPR chip containing high ratio of Ag may be applicable to high sensitive detection due to their sharp reflectance curves in intensity interrogation mode.

• Journal of Sensor Science and Technology
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• v.19 no.6
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• pp.456-461
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• 2010

The detection of C-reactive protein(CRP) using self-assembled monolayer(SAM) was investigated by a portable surface plasmon resonance(SPR) sensor system. The CRP is a biomarker for the possible cardiovascular disease. The SAM was formed on gold(Au) surface to anchor the monoclonal antibody of CRP(anti-CRP) for detection of CRP. Sequence injection of the anti-CRP and bovine serum albumin(BSA) into the sensor system has been carried out immobilize the antibody and to prevent non-specific binding. The portable SPR system has two flow channels: one for the sample measurements and the other for the reference. The output SPR signal was increased with the injection of the anti-CRP, BSA and CRP due to binding of the proteins on the sensor chip. The valid output SPR signals was linearly related to the critical range of the CRP concentration. The experimental results showed the feasibility of the portable SPR system with newly developed SAM to diagnose a risk of the future cardiovascular events.

• Journal of the Optical Society of Korea
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• v.11 no.2
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• pp.55-58
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• 2007

We propose a systematic method for design of fiber-optic surface plasmon resonance (SPR) sensors. We used rigorous coupled wave analysis (RCWA) for analysis of the transmission spectrum, and the (1+1) evolution strategy (ES) was employed as an optimization tool. The simulation results show that the optimization method presented here is very useful in designing fiber-optic SPR sensor for strain and temperature measurement. This algorithm can be extended to another objective function with other weighting factors and optical parameters.

• Journal of Sensor Science and Technology
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• v.20 no.6
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• pp.434-438
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• 2011

A portable surface plasmon resonance(SPR) based immunosensor using thiolated protein G and protein G was developed for the detection of immunoglobulin G(IgG). The protein G has specific affinity with Fc fragment of IgG and was thiolated by 2-Iminothiolane for introduction of thiol groups. Anti-IgG, bovine serum albumin(BSA), and IgG have been sequently injected after surface modification of gold sensor chip with protein G and thiolated protein G. The output signal was increased with the injection of each protein and the actual signal was measured by subtracting signal of reference channel from signal of sample injected channel. The experimental results showed the higher detection capability of IgG using thiolated protein G compared with protein G. From these results, we can conclude that the current surface modification technique and the portable SPR sensor system can be applied to various immunosensors for diagnosis.

• Korean Journal of Optics and Photonics
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• v.25 no.2
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• pp.95-101
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• 2014

When voltage is applied on the metal layer of a surface plasmon resonance (SPR) sensor, electric field excitation causes charge accumulation on the metal surface. This alters not only the optical properties of the metal but also the SPR angle. In this study we investigate this effectby performing experiments using solutions of various pH values, and we obtain the relation between total surface charge and SPR angle. The curves for the various pH conditions become coincident. We compare our results to those from an earlier space charge layer (SCL) model, and suggest a modified SCL model which explains our result well. This result will be useful in applications of SPR sensors, and in studying the optical properties of thin metal layers.

• Journal of Biosystems Engineering
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• v.34 no.1
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• pp.50-56
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• 2009

Surface plasmon resonance (SPR) biosensor has been used to detect many biochemical reactions, because this label-free sensor has high sensitivity and rapid response. The reactions are monitored by refractive index changes of the SPR biosensor. Iprovalicarb is protective, curative, and eradicative systemic fungicide introduced by Bayer AG in 1999. It has potential far control of downy mildew infesting onion, cucumber, grape and melon, late blight infesting tomato and potato, and anthracnose infesting watermelon and pepper. It is strictly limited to the maximum residue limit. In this study, the applicability of a portable SPR biosensor (Spreeta, Texas instrument, TX, USA) to detect the iprovalicarb residue was examined. The sensor chip was adopted to detect the reaction of iprovalicarb to immobilized iprovalicarb-antibody. The binding of the iprovalicarb onto the biosensor surface was measured by change of the refractive index (RI). Characteristics of the sensor chip including specificity, sensitivity, stability, and reusability were analyzed. In calibration test for seven levels of iprovalicarb concentration (0.32 to 5,000 mg/L) with three replications, a Sigmoidal model with Hill function was obtained between relative RI value and the iprovalicarb concentration with R-square of 0.998. It took 30 minutes to complete a set of detecting assay with the SPR biosensor.