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INHIBITION OF BROWNING REACTIONS OCCURRING IN THE STORAGE OF DRIED OYSTER 2. Inhibitory Effect of Sodium Sulfite Treatment and the Addition of Antioxidants (건조굴 저장중의 갈변방지 2. 아황산소오다 처리 및 항산화제 효과)

  • CHOI Jin-Ho;LEE Kang-Ho;KIM Mu-Nam
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.10 no.1
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    • pp.17-22
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    • 1977
  • In the present paper, the effect of sodium sulfite treatment on tile inhibition of browning reactions occurring in the storage of dried oyster was tested and the supplementary effect of antioxidantsaddedwasalsomentioned. Dried oysters treated with sodium sulfite solutions as described in the previous paper(Lee and Choi, 1977) were stored in the bottles with silica gel bags at room temperature with or without the application of antioxidants. The ethanol solution of an antioxidant mixture(BHA, BHT, plus, synergists) was sprayed on the surface of cooked oyster before drying. The density of brown pigment was determined spectrophotometrically by measuring the absorbance at 420 and 440 nm of both fractions of pigment extract, namely chloroform-methanol and water soluble fractions, which represent the brown color developed by fat oxidation and Maillard reactions respectively. TBA value was also measured for the oxidative rancidity in oysters during the storage. It appeared from the results that the 0.5 M sodium sulfite-60minute treated samples showed better effect after 150 day storage at room temperature. Controlling tile pH of treating solutions, did not reveal so much different in inhibitory effect in the aspect of color but a more reduction of tyrosine and reducing sugar was resulted with acidic solution than with alkaline solution. The development of brown color in dried oyster seemed to be leaded rather by the oxidative rancidity of lipids than sugar-amino reactions particularly in a long-term storage since the browning of chloroform-methanol fraction progressed more rapidly than of water. soluble fraction. The application of antioxidant, therefore, could largely retard the browning of the product as appeared in the results that sodium sulfite treated oyster with addition of antioxidant kept the best color during the storage.

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Effects of Hyperbaric Pressure on Cellular Morphology, Proliferation and Protein Expression of Jurkat Cell

  • Oh, Eun-Ha;Oh, Sang-Nam;Im, Ho-Sub;Lee, Joo-Hyun;Kim, Jin-Young;Moon, Joo-Hee;Hong, Eun-Young;Kim, Yang-Hee;Yang, Min-Ho;Lim, Yong-Chul;Park, Sun-Young;Lee, Eun-Il;Sul, Dong-Geun
    • Molecular & Cellular Toxicology
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    • v.1 no.2
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    • pp.116-123
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    • 2005
  • The application of high pressure on cellular morphology, proliferation and protein expression of Jurkat cells (human T lymphocyte cell line) has been extensively investigated. In the present study, we manufactured a novel pressure chamber that modulates 5% $CO_{2}$, temperature and pressure (up to 3 ATA). Jurkat cells was incubated 2 ATA pressure and analyzed cellular morphology and growth using an electron microscopy and MTT assay. The cells showed the morphological changes in the cell surface, which appeared to cause a severe damage in cell membrane. The growth rate of the cells under 2 ATA pressure decreased as cultured time got increased. Furthermore, a long term exposure of high pressure on Jurkat cells may act as one of the important cellular stresses that leads to inducing cell death. Cellular proteomes were separated by 2-dimensional electrophoresis with pH 3-10 ranges of IPG Dry strips. And many proteins showed significant up-and-down expressions with hyperbaric pressure. Out of all, 10 spots were identified significantly using matrix-assisted laser desorption/ionization-time of fight (MALDI-TOF) mass spectrometry. We and found that 9 protein expressions were decreased and one protein, heat shock protein HSP 60, was increased in Jurkat cells under 2 ATA. Identified proteins were related to lipid metabolism and signal transduction.

Growth of Rice Plant and Salinity under Different Flooding Times and Days to Transplanting after Submerged Rotary in Saline Paddy (우척답에서 정지후 환수회수와 이앙시기에 따른 염분농도와 수도생육)

  • 정진일;유숙종
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.38 no.5
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    • pp.398-404
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    • 1993
  • This study was conducted in order to obtain the information for yield improvement in saline paddy. Saline concentration, growth and yield of rice, being subjected to different flooding times and days to transplanting were investigated in saline paddy with 0.35 % and 0.5 % salt concentration. Saline concentration of soil was increased to 0.41 % just after rotary in the paddy with 0.35 % salinity, but decreased to 0.20 % after 3 to 4 times of flooding treatment. And also that of surface water was decreased from 0.2 % to 0.11 %. Saline concentration of soil in 5cm depth was decreased to 0.31 % by one time flooding and to 0.22% by 3~4 times flooding but salinity below 7cm depth showed slight decrease. Seedling death was exceeded 37 % when transplanted one day after rotary in the paddy with 0.35% salinity. Death ratio was decreased to 20% by three times flooding and transplanting six days after rotary. In paddy with 0.5 % salinity, death ratio was high but the tendency was very similar to 0.35 % field. In 0.35 % saline paddy field, yields were increased by 14 % by three times flooding and transplanting six days after rotary as compared to one time flooding and transplanting are day after rotary. Therefore, 3 to 4 times flooding and transplanting 5 to 6 days after rotary are desirable in high saline paddy.

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Hydrogeochemical study of a watershed in Pocheon area: controls of water chemistry

  • Kim, Kyoung-Ho;Yun, Seong-Taek;Chae, Soo-Ho;Jean, Jong-Wook;Lee, Jeong-Ho;Kweon, Hae-Woo
    • Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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    • 2004.09a
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    • pp.121-121
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    • 2004
  • The groundwater in the Pocheon area occurs from both a fractured bedrock aquifer in igneous and metamorphic rocks and an alluvial aquifer with a thickness of <50 m, and forms a major source of domestic and agricultural water supply. In this study, we performed a hydrogeochemical study in order to identify the control of geochemical processes on groundwater quality. For this study, groundwater level and physicochemical parameters (EC, Eh, pH, alkalinity) were monitored once a month from a total of 150 groundwater wells between June 2003 to August 2004. A total of 153 water samples (13 surface water, 66 alluvial groundwater, 74 bedrock groundwater) were also collected and analyzed in February 2004. Groundwater chemistry in the study area is very complex, depending on a number of major factors such as geology, degree of chemical weathering, and quality of recharge water. Hydrochemical reactions such as the leaching of surficial and near-solace soil salts, dissolution of calcite, cation exchange, and weathering of silicate minerals are proposed to explain the chemistry of natural groundwater. Alluvial groundwaters locally have very high TDS concentrations, which are characterized by their chloride(nitrate)-sulfate-bicabonate facies and low Na/Cl ratio. Their grondwater levels are highly fluctuated according to rainfall event. We suggest that high nitrate content and salinity in such alluvial groundwaters originates from the local recharge of sewage effluents and/or fertilizers. Likewise, high concentrations of nitrate were also locally observed in some bedrock groundwaters, suggesting their effect of anthropogenic contamination. This is possibly due to the bypass flow taking place through macropores. Tile degree of the weathering of silicate minerals seems to be a major control of the distribution of major cations (sodium, calcium, magnesium, potassium) in bedrock groundwaters, which show a general increase with increasing depth of wells. Thermodynamic interpretation of groundwater chemistry shows that the groundwater in the study area is in chemical equilibrium with kaolinite and Na-montmorillonite, which indicates that weathering of plagioclase to those minerals is a major control of hydrochemistry of bedrock groundwater. The interpretation of the molar ratios among major ions, as well as the mass balance calculation, also indicates the role of both dissolution/precipitation of calcite and Ca-Na cationic exchange as bedrock groundwaters evolves progressively.

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Mechanical reliability of Sn-37Pb BGA solder joints with high-speed shear test (고속전단 시험을 이용한 Sn-37Pb BGA solder joints의 기계적 신뢰성 특성 평가)

  • Jang, Jin-Kyu;Ha, Sang-Su;Ha, Sang-Ok;Lee, Jong-Gun;Moon, Jung-Tak;Park, Jai-Hyun;Seo, Won-Chan;Jung, Seung-Boo
    • Journal of the Microelectronics and Packaging Society
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    • v.15 no.4
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    • pp.65-70
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    • 2008
  • The mechanical shear strength of BGA(Ball Grid Array) solder joints under high impact loading was investigated. The Sn-37Pb solder balls with a diameter of $500{\mu}m$ were placed on the pads of FR-4 substrates with ENIG(Electroless Nickel Immersion Gold) surface treatment and reflowed. For the High Temperature Storage(HTS) test, the samples were aged a constant testing temperature of $120^{\circ}C$ for up to 250h. After the HTS test, high speed shear tests with various shear speed of 0.01, 0.1, 1, 3 m/s were conducted. $Ni_3Sn_4$ intermetallic compound(IMC) layer was observed at the solder/Ni-P interface and thickness of IMC was increased with aging process. The shear strength increased with increasing shear speed. The fracture surfaces of solder joints showed various fracture modes dependent on shear speed and aging time. Fracture mode was changed from ductile fracture to brittle fracture with increasing shear speed.

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Optimization of Extraction Conditions for Cabbage (홍월적 양배추의 추출조건 최적화)

  • Do, Jeong-Ryong;Kim, Hyun-Ku;Hong, Joo-Heon;Lee, Gee-Dong
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.34 no.10
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    • pp.1625-1632
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    • 2005
  • This study was conducted to monitor the extraction yields and functional properties from cabbage by a response surface methodology. The extract yield was maximized as 44.47$\%$ under the temperature of 79.86$^{\circ}C$, ethanol concentration of 56.84$\%$ and solvent to sample ratio 25.58 mL/g . The maximum value of electron donating ability was 85.46$\%$ at 46.38$^{\circ}C$,57.06$\%$ of ethanol concentration and 27.71 mL/g of solvent to sample ratio. The maximum value of tyrosinase inhibitory effect was 69.37$\%$ at 37.5$^{\circ}C$,47.71$\%$ of ethanol concentration and 16.03 mL/g of solvent to sample ratio. The maximum value of SOD-like activity was 48.36$\%$ in 66.12$^{\circ}C$, 70.35$\%$ of ethanol concentration and 29.13 mL/g of solvent to sample ratio. Estimated conditions for the maximized extraction including yield, electron donating ability and SOD-like activity were 20 $\∼$ 30 mL/g in ratio of solvent to sample, 25$\∼$85$\%$ in ethanol concentration, and 40$\∼$90$^{\circ}C$ in extraction temperature.

Case Studies of Geophysical Mapping of Hazard and Contaminated Zones in Abandoned Mine Lands (폐광 부지의 재해 및 오염대 조사관련 물리탐사자료의 고찰)

  • Sim, Min-Sub;Ju, Hyeon-Tae;Kim, Kwan-Soo;Kim, Ji-Soo
    • The Journal of Engineering Geology
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    • v.24 no.4
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    • pp.525-534
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    • 2014
  • Environmental problems typically occurring in abandoned mine lands (AML) include: contaminated and acidic surface water and groundwater; stockpiled waste rock and mill tailings; and ground subsidences due to mining operations. This study examines the effectiveness of various geophysical techniques for mapping potential hazard and contaminated zones. Four AML sites with sedimentation contamination problems, acid mine drainage (AMD) channels, ground subsidence, manmade liner leakage, and buried mine tailings, were selected to examine the applicability of various geophysical methods to the identification of the different types of mine hazards. Geophysical results were correlated to borehole data (core samples, well logs, tomographic profiles, etc.) and water sample data (pH, electrical conductivity (EC), and heavy metal contents). Zones of low electrical resistivity (ER) corresponded to areas contaminated by heavy metals, especially contamination by Cu, Pb, and Zn. The main pathways of AMD leachate were successfully mapped using ER methods (low anomaly peaks), self-potential (SP) curves (negative peaks), and ground penetrating radar (GPR) at shallow penetration depths. Mine cavities were well located based on composite interpretations of ER, seismic tomography, and well-log records; mine cavity locations were also observed in drill core data and using borehole image processing systems (BIPS). Damaged zones in buried manmade liners (used to block descending leachate) were precisely detected by ER mapping, and buried rock waste and tailings piles were characterized by low-velocity zones in seismic refraction data and high-resistivity zones in the ER data.

Vertical Distribution of Bulk Density and Salts in a Plastic Film House Soil (깊이별 용적밀도가 다른 시설재배지 토양의 염류분포)

  • Kim, Pil-Joo;Lee, Do-Kyoung;Chung, Doug-Young
    • Korean Journal of Soil Science and Fertilizer
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    • v.30 no.3
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    • pp.226-233
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    • 1997
  • To investigate the salt accumulation in the plastic film house soils, bulk density, electrical conductivity(EC), exchangeable canons and water soluble anions were determined at different depths(0~60cm) in the salt-accumulated plastic film house soils in Yesan, Chungnam, Korea. Bulk density were increased from $1.2Mg/m^3$ to $1.5Mg/m^3$ as the depth changed from 0cm(top soil) to 30cm(subsoil) below the soil surface, whereas the bulk densities between 30cm to 60cm slightly decreased to $1.42Mg/m^3$. These changes of soil bulk densities might influence the porosity and pore size distribution, resulting in affecting the water flow throughout, soil layers. Electrical conductivity and Exchangeable sodium percentage(ESP) for 0 to 10cm soil layer were 5.08 dS/m and 6.4, respectively, while the EC was decreased to less than 1.63 dS/m in 20~30cm depth and about 0.7 dS/m. Salt accumulation patterns in the plastic film house soils might be influenced by the changes of the bulk densities in soil.

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Allelopathic Effect of Sorghum Extract and Residues on Selected Crops and Weeds (수수의 타감작용(他感作用)에 관(關)한 연구(硏究))

  • Kim, S.Y.;De Datta, S.K.;Robles, R.P.;Kim, K.U.;Lee, S.C.;Shin, D.H.
    • Korean Journal of Weed Science
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    • v.14 no.1
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    • pp.34-41
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    • 1994
  • To better understand the allelopathic effect of sorghum(Sorghum vulgare L.), the inhibitory activities of water extracts of the stem, leaf and root, and of residues of the stem to major crops and weeds associated with them were evaluated. The allelopathic activity of sorghum plants was species specific, and depended on source and concentration. Germination, and shoot and root length of all test species were inhibited by the different concentrations of the stem extract. Among the crop species, radish showed the most inhibition, followed by wheat and rice. Maize was the least sensitive species. Of the weed species, Ipomoea triloba was most inhibited, followed by Echinochloa colona and Rottboellia cochinchinensis. The water extracts of leaves, stems, and roots significantly inhibited germination and seedling growth in E. colona and radish. The stem extract gave the greatest inhibitory effect on E. colona while all three extracts produced similar response in radish. In the greenhouse trial, sorghum stem residue placed on the soil surface as mulch significantly inhibited seedling growth in E. colona and radish, but not that in rice.

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Culture of the Olive Flounder, Paralichthys olivaceus in a Marine Recirculating System (해수 순환 여과 사육 시스템에서 넙치 사육 시험)

  • Bang, Jong-Deuk;Choi, Yong-Suk;Seo, Hyung-Chul
    • Journal of fish pathology
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    • v.18 no.1
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    • pp.91-97
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    • 2005
  • Bio-secure culture of olive flounder Paralichthys olivaceus in the IBK (Intensive Bioproduction Korean) recirculating system with dry pellet was tested for 6 months. The IBK system consists of 12 rearing tanks, 6 sedimentation tanks. 4-sectioned submerged biofilter chamber and channels. The size of each rearing tank was 3m in diameter and 1m in depth. The size of each biofilter chamber was $3.1\times3.3\times2.0$ m (D) and was filled with corrugated plastic plates as a biofilter medium. Total surface area of the biofilter was 3,789.7 $m^2$ Water was circulated by one of two vertical axial pump and circulating rate was about 34 times per day. A UV light sterilizer was used to treat inlet sea water with the flow rate of 4 ton/hr. All fish were treated with 150 ppm formalin 3 times with 5 day interval before stocking. It took 60 days for 'conditioning' the biofilter with the stocking density of 4.5 kg of fish $m^2$. The concentrations of ammonium-nitrogen, nitrite-nitrogen and nitrate-nitrogen in the system remained at the range of 0.096-0.315 mg/L, 0.015-0.504 mg/L, and 2.530-39.517 mg/L, respectively. Water temperature fluctuated from 17.5 to 25.1$^{\circ}C$ and salinity was from 30.1 to 33.5 ppt during rearing period. The fish grew from the average weight of 615.2 g to 1,201.1 g for 180 days. Initial and final fish densities were 8.4 and 15.9$kg/m^2$, Survival rate was 97.1 %. Neither parasites nor noticeable diseases was observed during the raring period even Vibrio spp. were detected from some fish in the system.