Molecular & Cellular Toxicology

The Korean Society of Toxicogenomics and Toxicoproteomics (대한독성유전 단백체학회)
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Effects of Hyperbaric Pressure on Cellular Morphology, Proliferation and Protein Expression of Jurkat Cell

  • Oh, Eun-Ha (Environmental Toxico-Gemomic and Proteomic Center, School of Medicine, Korea University) ;
  • Oh, Sang-Nam (Environmental Toxico-Gemomic and Proteomic Center, School of Medicine, Korea University) ;
  • Im, Ho-Sub (School of Public Health, Korea University) ;
  • Lee, Joo-Hyun (School of Public Health, Korea University) ;
  • Kim, Jin-Young (School of Public Health, Korea University) ;
  • Moon, Joo-Hee (School of Public Health, Korea University) ;
  • Hong, Eun-Young (School of Public Health, Korea University) ;
  • Kim, Yang-Hee (Environmental Toxico-Gemomic and Proteomic Center, School of Medicine, Korea University) ;
  • Yang, Min-Ho (Environmental Toxico-Gemomic and Proteomic Center, School of Medicine, Korea University) ;
  • Lim, Yong-Chul (Environmental Toxico-Gemomic and Proteomic Center, School of Medicine, Korea University) ;
  • Park, Sun-Young (School of Public Health, Korea University) ;
  • Lee, Eun-Il (Environmental Toxico-Gemomic and Proteomic Center, School of Medicine, Korea University) ;
  • Sul, Dong-Geun (Environmental Toxico-Gemomic and Proteomic Center, School of Medicine, Korea University)
  • Published : 2005.06.30
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Abstract

The application of high pressure on cellular morphology, proliferation and protein expression of Jurkat cells (human T lymphocyte cell line) has been extensively investigated. In the present study, we manufactured a novel pressure chamber that modulates 5% $CO_{2}$, temperature and pressure (up to 3 ATA). Jurkat cells was incubated 2 ATA pressure and analyzed cellular morphology and growth using an electron microscopy and MTT assay. The cells showed the morphological changes in the cell surface, which appeared to cause a severe damage in cell membrane. The growth rate of the cells under 2 ATA pressure decreased as cultured time got increased. Furthermore, a long term exposure of high pressure on Jurkat cells may act as one of the important cellular stresses that leads to inducing cell death. Cellular proteomes were separated by 2-dimensional electrophoresis with pH 3-10 ranges of IPG Dry strips. And many proteins showed significant up-and-down expressions with hyperbaric pressure. Out of all, 10 spots were identified significantly using matrix-assisted laser desorption/ionization-time of fight (MALDI-TOF) mass spectrometry. We and found that 9 protein expressions were decreased and one protein, heat shock protein HSP 60, was increased in Jurkat cells under 2 ATA. Identified proteins were related to lipid metabolism and signal transduction.

Keywords

References

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