• Journal of Medicine and Life Science
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• v.15 no.2
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• pp.46-50
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• 2018

Cancer, a leading mortality disease following cardiovascular disease worldwide, has high incidence as one out of every four adults in Korea. It was known to be caused by several reasons including somatic mutation, activation of oncogene and chromosome aneuploidy. Cancer cells show a faster growth rate and have metastatic and heterogeneous cell populations compared to normal cells. Cancer stem cells, the most invested field in cancer biology, is a theory to explain heterogeneous cell populations of cancer cells among several characteristics of cancer cells, which is providing the theoretical background for incidence of cancer and treatment failure by drug resistance. Cancer stem cells initially explain heterogeneous cell populations of cancer cells based on the same markers of normal stem cells in cancer, in which only cancer stem cells showed heterogeneity of cancer cells and tumor initiating ability of leukemia. Based on these results, cancer stem cells were reported in various solid cancers such as breast cancer, liver cancer, and lung cancer. Breast cancer stem cells were first reported in solid cancer which had tumor initiating ability and further identified as anti-cancer drug resistance. There were several identification methods in breast cancer stem cells such as specific surface markers and culture methods. The discovery of cancer stem cells not only explains heterogeneity of cancer cells, but it also provides theoretical background for targeting cancer stem cells to complete elimination of cancer cells. Many institutes have been developing new anticancer drugs targeting cancer stem cells, but there have not been noticeable results yet. Many researchers also reported a necessity for improvement of current concepts and methods of research on cancer stem cells. Herein, we discuss the limitations and the perspectives of breast cancer stem cells based on the current concept and history.

• Journal of Periodontal and Implant Science
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• v.51 no.5
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• pp.329-341
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• 2021

Purpose: Periodontal treatment aims at complete regeneration of the periodontium, and developing strategies for periodontal regeneration requires a deep understanding of the tissues composing the periodontium. In the present study, the stemness characteristics and gene expression profiles of cementum-derived cells (CDCs) were investigated and compared with previously established human stem cells. Candidate marker proteins for CDCs were also explored. Methods: Periodontal ligament stem cells (PDLSCs), pulp stem cells (PULPSCs), and CDCs were isolated and cultured from extracted human mandibular third molars. Human bone marrow stem cells (BMSCs) were used as a positive control. To identify the stemness of CDCs, cell differentiation (osteogenic, adipogenic, and chondrogenic) and surface antigens were evaluated through flow cytometry. The expression of cementum protein 1 (CEMP1) and cementum attachment protein (CAP) was investigated to explore marker proteins for CDCs through reverse-transcription polymerase chain reaction. To compare the gene expression profiles of the 4 cell types, mRNA and miRNA microarray analysis of 10 samples of BMSCs (n=1), PDLSCs (n=3), PULPSCs (n=3), and CDCs (n=3) were performed. Results: The expression of mesenchymal stem cell markers with a concomitant absence of hematopoietic markers was observed in PDLSCs, PULPSCs, CDCs and BMSCs. All 4 cell populations also showed differentiation into osteogenic, adipogenic, and chondrogenic lineages. CEMP1 was strongly expressed in CDCs, while it was weakly detected in the other 3 cell populations. Meanwhile, CAP was not found in any of the 4 cell populations. The mRNA and miRNA microarray analysis showed that 14 mRNA genes and 4 miRNA genes were differentially expressed in CDCs vs. PDLSCs and PULPSCs. Conclusions: Within the limitations of the study, CDCs seem to have stemness and preferentially express CEMP1. Moreover, there were several up- or down-regulated genes in CDCs vs. PDLSCs, PULPSCs, and BMSCs and these genes could be candidate marker proteins of CDCs.

• Korean Journal of Biological Psychiatry
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• v.22 no.2
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• pp.47-54
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• 2015

In the past decade, structural, molecular, and functional changes in glial cells have become a major focus in the search for the neurobiological foundations of schizophrenia. Glial cells, consisting of oligodendrocytes, astrocytes, microglia, and nerve/glial antigen 2-positive cells, constitute a major cell population in the central nervous system. There is accumulating evidence of reduced numbers of oligodendrocytes and altered expression of myelin/oligodendrocyte-related genes that might explain the white matter abnormalities and altered inter- and intra-hemispheric connectivities that are characteristic signs of schizophrenia. Astrocytes play a key role in the synaptic metabolism of neurotransmitters ; thus, astrocyte dysfunction may contribute to certain aspects of altered neurotransmission in schizophrenia. Increased densities of microglial cells and aberrant expression of microglia-related surface markers in schizophrenia suggest that immunological/inflammatory factors are of considerable relevance to the pathophysiology of psychosis. This review describes current evidence for the multifaceted role of glial cells in schizophrenia and discusses efforts to develop glia-directed therapies for the treatment of the disease.

• Proceedings of the KOSOMBE Conference
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• v.1994 no.05
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• pp.32-34
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• 1994

An automatic multiframe procedure for tracking signalized points on multiple, differently moving discontinuous surface is described. The position of markers which attached on human body give us some important information such as velocity, acceleration, angular velocity and angular acceleration, etc, of the rigid objects. Generally, the detected objects occufying some area in images rather than a point because of the generic size of the marks or the smeared effects of the camera. To solve the problem we used a modified clustering algorithm.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2003.06a
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• pp.256-259
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• 2003

This paper proposes OPTICAL MARKER fer registering process - one of the 3D measurement process : scan registering - merging - measurement. If the registering work is carried out manually, it can be accompanied with much time and many errors. Because the patterned marker make registering process automatic, many firms use it now. But the physical shape of existing markers is the source of the data loss caused by hiding surface, and the marker arrangement is the source of the time loss. The optical marker proposed in this paper has marker generator, organized a large number of binary coded control laser diode, separate from 3D scan object. So, it does not take much time for the marker disposition, and it is not the origin of the data loss, and the binary coded laser information make the auto-registering possible.

• Korean Journal of Clinical Laboratory Science
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• v.44 no.3
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• pp.142-146
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• 2012

Hepatitis B surface antigen (HBsAg) seroclearance is a rare event in chronic hepatitis B virus (HBV) infection which acquires the disease early in life. A case study have examined with asymptomatic chronic hepatitis B carrier who exhibits HBsAg seroclearance in anti-HBe positive. We comprehensively studied the biochemical, virological and clinical aspects of a patient with HBsAg seroclearance. Liver biochemistry, serological markers, serum HBV DNA levels, and development of clinical complications were monitored. Mutation of hepatitis B virus is suspected serum HBsAg detected by the HBsAg assay systems of VITROS (OrthoClinical Diagnostics, USA), AxSYM (Abbott Laboratories, USA), Elecsys (Roche Diagnostics, Germany) and ADVIA Centaur (Bayer Diagnostics, USA). These four immunoassays showed negative results. Also, the patient had undetectable serum HBV DNA. Therefore, no mutation within the "a" determinant of HBsAg, which might escape detection from HBsAg immunoassay were found. Natural seroclearance was confirmed.

• Journal of the Korean Society of Safety
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• v.21 no.6 s.78
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• pp.82-87
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• 2006

The existing raised pavement marker, however, have some limitations. Most of them are square-shaped which makes only forward and backward retro-reflection, their orientation can be easily changed by impact of vehicle, and the surface reflector can be readily contaminated leading poor functioning. In addition, maintenance for broken raised pavement marker is not easy. The purpose of the study is to design and manufacture a new and improved type of raised pavement marker. The new raised pavement marker has round-shaped upper structure. Its body was separated from the holder and the reflector was surrounded by acrylic cover. We tested performances of the constituent parts, optimum load after installation, and reflection efficiency. The new raised pavement marker will be an initiation of studies on the improvement of the shape and performance of raised pavement markers.

• Proceedings of the KSME Conference
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• 2008.11a
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• pp.979-983
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• 2008

A bi-modal tram can travel in not only dedicated way but also road so as to reduce construction costs and increase vehicle operation efficiency, whose passenger capacity is 2,500 to 7,000 persons/direction/hour. A bi-modal has an electronic guidance system that knows the location and route of the vehicle, and uses magnetic markers in the road surface for reference. Since a bi-modal tram will be operated in the downtown area, there is some possibility that strong wind occurred between high-rise buildings can produce sudden lateral movement (displacement) of the vehicle to influence its automatic operation controlled by electronic guidance system. For bi-modal tram in the automatic operation mode, lateral movements occurred by strong wind were calculated and analyzed in the dynamic model developed by using the ADAMS. Some useful relations among vehicle speeds, wind speeds, and lateral behaviors were discussed in this paper.

• Korean Journal of Plant Resources
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• v.32 no.1
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• pp.19-28
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• 2019

An efficient shoot regeneration condition for pea cv. 'Sparkle' was developed by using optimum explant, plant growth regulator concentrations, and pretreatment of BA onto explant. The average shoot number per explant showed the highest on two kinds of shoot induction media (MSB5 media containing 2 mg/L BA and a combination of 2 mg/L BA and 1 mg/L TDZ) when cotyledonary node explants were cultured. Moreover, the pretreatment of explant in 200 mg/L BA solution was found to be more effective in shoot induction than that of non-pretreatment. By histological study, cell division and proto-meristem were formed near the surface of the sub-epidermal and epidermal cell layers of cotyledonary node in earlier than 3 days after culture. The analysis of genetic stability of regenerants by using thirteen ISSR markers showed that in vitro regenerated plants showed polymorphism with 8.3% compared with their mother plants.

• The Korean Journal of Mycology
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• v.52 no.2
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• pp.135-143
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• 2024

Lindera obtusiloba Blume, a shrub prevalent in the understory vegetation of the deciduous forests in East Asia and Korea, is known for its pharmacological properties, including antioxidant, anti-allergic, and anti-inflammatory activities. Endophytic fungi were isolated from surface-sterilized twigs and leaves of L. obtusiloba collected from Samcheok-si and Jecheon-si in Korea. The fungal strains were identified based on their morphological characteristics and molecular analyses using DNA markers such as internal transcribed spacer, large subunit rDNA, β-tubulin, chitin synthase, and glyceraldehyde-3-phosphate dehydrogenase. Here, we report two new records of endophytic fungi isolated from L. obtusiloba in Korea: Colletotrichum citricola and Valsa ceratophora. These results enhance our understanding of the diversity of endophytic fungi in L. obtusiloba and contribute to enhancing our knowledge of fungal biodiversity in Korea.