• Journal of Microbiology and Biotechnology
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• 제21권2호
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• pp.129-135
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• 2011

Cloning and characterization of the gene encoding a solvent-tolerant protease from the haloalkaliphilic bacterium Geomicrobium sp. EMB2 are described. Primers designed based on the N-terminal amino acid sequence of the purified EMB2 protease helped in the amplification of a 1,505-bp open reading frame that had a coding potential of a 42.7-kDa polypeptide. The deduced EMB2 protein contained a 35.4-kDa mature protein of 311 residues, with a high proportion of acidic amino acid residues. Phylogenetic analysis placed the EMB2 gene close to a known serine protease from Bacillus clausii KSM-K16. Primary sequence analysis indicated a hydrophobic inclination of the protein; and the 3D structure modeling elucidated a relatively higher percentage of small (glycine, alanine, and valine) and borderline (serine and threonine) hydrophobic residues on its surface. The structure analysis also highlighted enrichment of acidic residues at the cost of basic residues. The study indicated that solvent and salt stabilities in Geomicrobium sp. protease may be accorded to different structural features; that is, the presence of a number of small hydrophobic amino acid residues on the surface and a higher content of acidic amino acid residues, respectively.

• 한국안전학회지
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• 제27권3호
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• pp.9-14
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• 2012

The objectives of this study were to compare the results of HSL (Health and Safety Laboratory) ramp test with perceived sense of slip onto the several different floor surfaces under contaminated conditions. There are a variety of approaches from biomechanical measurements to psychophysical test and human perception. However, controversies over these approaches still remain. Some widely accepted methods need to be improved. AHP (Analytic Hierarchy Process) was used to evaluate the perception of slipperiness of seven different floor surfaces under the contaminated condition with glycerol solution. Twelve subjects worn same footwear and walked with self-selected step and cadence along the test floors. The angle of inclination obtained for water wet condition using 5 l/min with HSL ramp test was compared to perception of slipperiness. The surface roughness ($R_z$) related very well both AHP (r=0.95) and ramp test (r=0.92). The high significant correlation (r=0.90) was found between AHP and HSL ramp test.The HSL ramp test values (Coefficient of Friction, COF) according to subjective evaluation were divided into two categories. There were high correlations between test results (subjective evaluation, HSL ramp test) and surface roughness in Rz. Perception rating obtained with AHP showed a high correlation with COF obtained with HSL ramp test.

• 한국공작기계학회논문집
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• 제17권5호
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• pp.52-57
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• 2008

Most of the electrons emitted from the filament, are captured by the anode. The portion of the electron current that leaves the gun through the hole in the anode is called the beam current. Electron beam probe is called the focused beam on the specimen. Because of the lenes and aperture, the probe current becomes smaller than the beam current. It generate various signals(backscattered electron, secondary electron) in an interaction with the specimen atoms. Backscattered electron provide an useful signal for composition and local specimen surface inclination. Secondary electron is used far the formation of surface imagination. The steady electron beam probe is very important for the imagination formation and the brightness. In this paper, we show the results of developed elements that create electron beam probe and the measured beam probe in various acceleration voltages by Faraday cup. These data are used to analysis and improve the performance of the system in the development.

• 한국태양에너지학회 논문집
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• 제24권3호
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• pp.19-25
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• 2004

The measured solar radiation incident on tilted surfaces by all directions has been widely used as important solar radiation data in installing photovoltaic modules. To maximize the incident beam radiation, the slope, which is the angle between the plane of the surface in question and the horizontal, an4 the solar azimuth angles are needed for these solar photovoltaic systems. This is because the performance of the solar photovoltaic systems is much affected by angle and direction of incident rays. Recognizing those factors mentioned above are of importance, actual experiment has been performed in this research to obtain the an91e of inclination with which the maximum incident rays can be absorbed. The results obtained in this research could be used in installing optimal photovoltaic modules.

• Current Optics and Photonics
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• 제4권6호
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• pp.509-515
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• 2020

A practical single photon source for fiber-based quantum information processing is still lacking. As a possible 1.55-㎛ quantum-dot single photon source, an InGaAsP/InP-air-aperture micropillar cavity is investigated in terms of fabrication tolerance. By properly modeling the processing uncertainty in layer thickness, layer diameter, surface roughness and the cavity shape distortion, the fabrication imperfection effects on the cavity quality are simulated using a finite-difference time-domain method. It turns out that, the cavity quality is not significantly changing with the processing precision, indicating the robustness against the imperfection of the fabrication processing. Under thickness error of ±2 nm, diameter uncertainty of ±2%, surface roughness of ±2.5 nm, and sidewall inclination of 0.5°, which are all readily available in current material and device fabrication techniques, the cavity quality remains good enough to form highly efficient and coherent 1.55-㎛ single photon sources. It is thus implied that a quantum dot contained InGaAsP/InP-air-aperture micropillar cavity is prospectively a practical candidate for single photon sources applied in a fiber-based quantum information network.

• 로봇학회논문지
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• 제17권1호
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• pp.48-57
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• 2022

In this paper, we propose a legged landing platform for the quadcopter taking off and landing in the ship environment. In the ship environment with waves and winds, the aircraft has risks being overturned by contact impact and excessive inclination during landing on the ship. This landing platform has four landing legs under the quadcopter for balancing and shock relief. In order to make the quadcopter balanced on ships, the position of each end effector was controlled by PID control. And shocks have mainly happened when quadcopter contacts the ship's surface as well as legs move fast. Hence, impedance control was used to cope with the shocks. The performance of the landing platform was demonstrated by a simulation and a prototype in three sea states based on a specific size of a ship. During landing and tracking the slope of the ship's surface, oscillations of rotation and translation from the shock were mitigated by the controller. As a result, it was verified that transient response and stability got better by adding impedance control in simulation models and prototype experiments.

• 한국기계가공학회지
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• 제21권5호
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• pp.91-97
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• 2022

The Directed Energy Deposition (DED) is a representative metal additive manufacturing method. Owing to its strong point of repairment, its application is gradually spreading in aerospace applications, power generation, military components, and mold making. 5-axis cladding is needed to repair damage, such as wear and scratches on cylindrical surfaces to circular-shaped parts, including sleeves and liners. Furthermore, the condition of cladding on inclined parts must also be considered to prevent interference between the nozzle and the part. In this study, the effects of changes in scanning speed due to the 5-axis control system and differences from the height of laser beam irradiation due to inclination are evaluated among the items that should be additionally considered in 5-axis cladding compared to 3-axis cladding. Moreover, the trends of the width and height of the clad are identified by different tilting angles via single line cladding. Lastly, cladding methods on cylindrical surfaces at various angles are proposed to enhance the clad quality and post-processing efficacy. These results can be applied with 5-axis cladding on inclined surfaces, including cylindrical surfaces.

• Geomechanics and Engineering
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• 제31권1호
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• pp.71-86
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• 2022

In Taiwan, an efficient approach for enhancing the stability of colluvium slopes is the drilled shaft method. For slopes with drilled shafts, the soil arching effect is one of the primary factors influencing slope stability and intertwines to the failure mechanism of the pile-soil system. In this study, the contribution of soil arching effect to slope stability is evaluated using the FEM software (Plaxis 3D) with the built-in strength reduction technique. The result indicates the depth of the failure surface is influenced by the S/D ratio (the distance to the diameter of piles), which can reflect the contribution of the soil arching effect to soil stability. When α (rock inclination angles)=β (slope angles) is considered and the S/D ratio=4, the failure surface of the slope is not significantly influenced by the piles. Overall, the soil arching effect is more significant on α=β, especially for the steep slopes. Additionally, the soil arching effect has been included in the proposed stability charts. The proposed charts were validated through two case studies, including that of the well-known Woo-Wan-Chai field in Taiwan. The differences in safety factor (FoS) values between the referenced literature and this study was approximately 4.9%.

• Geomechanics and Engineering
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• 제37권1호
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• pp.31-48
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• 2024

To stabilize the excavations in urban area, soil anchorage is among the very common methods in geotechnical engineering. A more efficient deformation analysis can potentially lead to cost-effective and safer designs. To this end, a total of 116 three-dimensional (3D) finite element (FE) models of a deep excavation supported by tie-back wall system were analyzed in this study. An initial validation was conducted through examination of the results against the Texas A&M excavation cases. After the validation step, an extensive parametric study was carried out to cover significant design parameters of tie-back wall system in deep excavations. The numerical results indicated that the maximum horizontal displacement values of the wall (δ_hm) and maximum surface settlement (δ_vm) increase by an increase in the value of ground anchors inclination relative to the horizon. Additionally, a change in the wall embedment depth was found to be contributing more to δ_vm than to δ_hm. Based on the 3D FE analysis results, two simple equations are proposed to estimate excavation deformations for different scenarios in which the geometric configuration parameters are taken into account. The model proposed in this study can help the engineers to have a better understanding of the behavior of such systems.

• 대한치과교정학회지
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• 제25권2호
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• pp.209-221
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• 1995

한국인에게 적합한 설측교정장치와 이상적인 설측 치열궁 아치와이어 제작의 기초자료를 얻기위하여 정상교합자 경석고 모형 30개를 재료로 하여 설측치관의 형태와, 후지타 설측 브라켓을 위치시킨 모형에서 설측치열궁 형태를 조사하여 다음과 같은 결과를 얻었다. 각 치아에 대한 치관의 근원심 경사도와 설면의 순설측 치관 경사도의 평균치를 얻었다. 각 치아에 대한 브라??? 위치에서 설면의 수평 및 수직적 형태를 얻은 결과 대구치, 소구치, 견치는 상하악이 비슷한 모양을 보였으며, 강하악의 중절치와 측절치도 비슷한 모양을 보여 각각 브라켓 기저부를 공유할 수 있을 것으로 나타났다. 브라켈 부착위치에서의 평균 설측치열궁 형태를 얻은 결과, 그 형태는 전치부는 반원형이며 견치와 소구치 사이에 강한 오프??? 밴드가 있는 버섯 형태로서 소구치간 및 제2소구치와 대구치간에도 오프??? 밴드가 필요한 것으로 나타났다. 남녀간의 평균 설측치열궁 형태는 상악은 거의 일치하였고 하악은 여자가 남자보다 다소 작은 형태를 나타내었다.