• Journal of Life Science
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• v.26 no.3
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• pp.353-358
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• 2016

Thiolase is an enzyme that catalyzes condensation reactions between two acetyl-CoA molecules to produce acetoacetyl-CoA. As thiolase catalyzes is the first reaction in the production of n-butanol, knowledge of the molecular and regulatory mechanism of the enzyme is crucial for synthesizing high-value biofuel. Thiolase from Clostridium butyricum (CbTHL) was expressed, purified, and crystallized. X-ray diffraction data were collected from the crystals, and the 3-dimentional structure of the enzyme was determined at 2.0 Å. The overall structure of thiolase was similar to that of type II biosynthetic thiolases, such as thiolase from C. acetobutylicum (CaTHL). The superposition of this structure with that of CaTHL complexed with CoA revealed the residues that comprise the catalytic and substrate binding sites of CbTHL. The catalytic site of CbTHL contains three conserved residues, Cys88, His349, and Cys379, which may function as a covalent nucleophile, general base, and second nucleophile, respectively. For substrate binding, the way in which CbTHL stabilized the ADP moiety of CoA was unlike that of other thiolases, whereas the stabilization of β-mercaptoethyamine and pantothenic acid moieties of CoA was quite similar to that of other enzymes. The most interesting observation in the CbTHL structure was that the enzyme was regulated through redox-switch modulation, using a reversible disulfide bond.

• International Journal of Highway Engineering
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• v.7 no.1 s.23
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• pp.49-61
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• 2005

The dynamic modulus of hot mix asphalt can be determined according to the different combinations of testing temperature and loading frequency. The superposition rule is adapted to get the master curve of dynamic modulus for each hot mix asphalt. There are couple of different methods to get the shift factor which is a key for making the master curve. In this paper, Arrehnius, 2002 AASHTO, and experimental method was employed to get the master curve. Evaluation of dynamic modulus for 25mm base course of hot mix asphalt with granite aggregate and two asphalt binders(AP-3 and AP-5) was carried out. Superpave Level 1 Mix Design with gyratory compactor was adopted to determine the optimum asphalt binder content(OAC) and the measured ranges of OAC were between 4.1% and 4.4%. UTM was used for laboratory test. The dynamic modulus and phase angle were determined by testing on UTM, with 5 different testing temperature(-10, 5, 20, 40, & $55^{\circ}C$) and 5 different loading frequencies(0.05, 0.1, 1, 10, 25 Hz). Using the measured dynamic modulus and phase angle, the input parameters of Sigmoidal function equation to represent the master curve were determined and these will be adopted in FEM analysis for asphalt pavements. The shift factor and activation energy for determination of master curve were calculated.

• Journal of the Korean Society of Radiology
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• v.7 no.2
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• pp.131-136
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• 2013

Panorama of dental radiation generators can observe the wide anatomical structures of oral and maxillofacial areas but there can be distortion of lengths, angles, or shapes. CBCT can diagnose 3D images and get the ones whose errors by superposition and interference are remarkably smaller between anatomical structures. But the quality of the images by movement of subjects can be lowered as it takes long to diagnose them. And if there are impermeable radiation objects like metal in mouths, impermeable radiation lines can radially appear with the objects as center. This study tries to analyze accuracy of panorama and CBCT and get useful anatomical information in dental treatment by comparing the length of wisdom teeth which were measured by Panorama and CBCT with the teeth which were actually extracted and analyzing distortion of the teeth. The test result could be found that Panorama is expanded by average 7.3% as the errors of Panorama and Digital Vernier Caliper range from 110.7% to 103.9%. The length of wisdom teeth which were measured in CBCT and Digital Vernier Caliper could be found that the error range is 1.3%. And the length of wisdom teeth which were measured in Panorama and Digital Vernier Caliper has found that the error range shows 7.3%. So it could be found that the images of CBCT is about 6% more exact than those of Panorama. It could be found that CBCT shows the more exact images than those of Panorama. But because the examination expenses of CBCT are higher than those of Panorama and exposure dose of CBCT is much more than that of Panorama, it is thought to find proper ways in examination.

• Journal of Korean Tunnelling and Underground Space Association
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• v.20 no.3
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• pp.625-639
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• 2018

This study is about the reinforcing type of reinforcement method which is reinforced in tunnel portal of tunnel with bad ground condition. Generally, it is known that the horizontal reinforcement method is more effective than the conventional reinforcement method. However, as a limitation of the tunnel construction technology, it is being constructed by the superposition reinforcement method. In recent years, high-strength large-diameter steel pipes and horizontally oriented longitudes (L = 30.0~50.0 m) construction technology have been developed. Therefore, it is required to study reinforcement method of tunnel portal reinforcement method. Therefore, 3-D numerical analysis (Midas GTS NX 3D) was performed by setting the reinforcement method (No reinforcement type, overlap reinforcement type and horizontal reinforcement type) and ground condition as parameters. As a result, it was considered that the reinforcement effect was the largest as the horizontal reinforcement type of the reinforcement method was the smallest in the displacement and the support material stress. Based on the results of the numerical analysis, horizontal steel pipe grouting was applied to the actual tunnel site. The displacement of the tunnel portal and the stress of the support material occurred within the allowable values and were considered to ensure sufficient stability.

• Journal of Convergence for Information Technology
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• v.10 no.10
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• pp.115-122
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• 2020

Due to the climate change, torrential rain downpours unprecedentedly, and urban areas repeatedly suffer from the inundation damages, which cause miserable loss of property and life by flooding. Two major reasons of urban flooding are river inundation and inland submergence. However, most of previous studies ignored the comprehensive mechanism of those two factors, and showed discrepancy and inadequacy due to the linear summation of each analysis result. In this study, river inundation and inland flooding were analyzed at the same time. Petrov-stabilizing scheme was adopted to capture the shock wave accurately by which river inundation can be modularized. In addition, flux-blocking alrotithm was introduced to handle the wet and dry phenomena. Sink/source terms with EGR (Exponentially Growth Rate) concept were incorporated to the shallow water equations to consider inland flooding. Comprehensive simulation implementing inland flooding and river inundation at the same time produced satisfactory results because it can reflect the counterbalancing and superposition effects, which provided accurate prediction in flooding analysis.

• Polymer(Korea)
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• v.25 no.3
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• pp.359-366
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• 2001

Isothermal physical aging of a glass fiber/epoxy composite was examined at different aging temperatures ($T_a$) and degrees of conversion (monitored by the glass transition temperature, $T_g$) by means of the TBA torsion pendulum technique. The range of aging temperature was from 10 to $130^{\circ}C$ : the conversion was systematically changed from $T_g$=$76^{\circ}C$ to $T_g$=$177^{\circ}C$ (fully crosslinked). The effect of isothermal physical aging was manifested as perturbations of the modulus and mechanical loss vs. temperature in the vicinity of $T_a$ for all conversions. The rate of isothermal physical aging determined from the change of modulus with aging time at fixed aging temperature decreased and then increased with increasing conversion below T$_{a}$=9$0^{\circ}C$. There exists a superposition in aging rate vs. ($T_g$ -$T_a$) by shifting horizontally and vertically. This implies that the physical aging process is independent of the change of chemical structure as conversion proceeds. It has been found that water absorbed at the aging temperature below $70^{\circ}C$ during isothermal physical aging lowers the apparent aging rate. It is due to the absorbed water molecules forming strong polar interactions with hydroxyl group on network chain and reducing the segmental mobility during the physical aging.g.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.34 no.4
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• pp.1203-1213
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• 2014

The hydraulic characteristics and the patterns of riverbed change had been analysed by HEC-RAS simulation in a reach of Cheong-mi river with and without weir. The corresponding physical disturbance had been evaluated with the method suggested by K-water (2008). The occurrences of low physical disturbance score coincide with the corresponding high bed changes by weir installation. The effects of physical disturbance coincide with the patterns of riverbed change along river reach which shows riverbed change is the important factor to physical disturbance. In case of installation of additional weirs at up and down stream sections of no disturbance effect by the existing weir, no physical disturbances occur in certain sections with confirming the appearance of the similar disturbance scores between the simulation results of with and without additional weir installations. In case of installation of additional weirs at up and down stream sections of disturbance effect margins by the existing weir, physical disturbances occur at every section. In case of installation of additional weirs at up and down stream sections within disturbance effect by the existing weir, low physical disturbance scores are given at every section because of superposition of disturbance along river reach. The physical disturbance would be minimized such that the additional weir is installed with sufficient distances of no disturbance and bed change effects along river reach.

• Journal of the Korean Chemical Society
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• v.59 no.5
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• pp.387-396
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• 2015

The DFT and ab initio calculations have been performed to elucidate hydrogen interaction of HOOO-(H₂O)_n (n=1~5) clusters. The optimized geometries, harmonic vibrational frequencies, and binding energies are predicted at various levels of theory. The trans conformer of HOOO monomer is predicted to be thermodynamically more stable than cis form at the CCSD(T) level of theory. For HOOO-(H₂O)_n clusters, the geometries are optimized at B3LYP/aug-cc-pVTZ and CAM-B3LYP/aug-cc-pVTZ levels of theory. The binding energy of HOOO-H₂O cluster is predicted to be 6.05 kcal/mol at the MP2//CAM-B3LYP/ aug-cc-pVTZ level of theory after zero-point vibrational energy (ZPVE) and basis set superposition error (BSSE) correction. The average binding energy per H₂O is increased according to adding a H₂O moiety in HOOO-(H₂O)_n clusters up to 7.2 kcal/mol for n=5.

• Journal of the Institute of Electronics Engineers of Korea TC
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• v.46 no.5
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• pp.26-35
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• 2009

OFDM (Orthogonal Frequency Division Multiplexing) system is robust to frequency selective fading and narrowband interference in high-speed data communications. However, an OFDM signal consists of a number of independently modulated subcarriers and the superposition of these subcarriers causes a problem that can give a large PAPR(Peak-to-Average Power Ratio). Phase rotation method can reduce the PAPR without nonlinear distortion by multiplying phase weighting factors. But computational complexity of searching phase weighting factors is increased exponentially with the number of subblocks and considered phase factor. Therefore, a new method, which can reduce computational complexity and detect phase weighting factors efficiently, should be developed. In this paper, a modeling process is introduced, which apply metaheuristic algerian in phase rotation method and optimize in PTS (Particle Swarm Optimization) scheme. Proposed algorithm can solve the computational complexity and guarantee to reduce PAPR We analyzed the efficiency of the PAPR reduction through a simulation when we applied the proposed method to telecommunication systems.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.5 no.1
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• pp.123-132
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• 1985

The flexibility of base material gives considerable influences on seismic responses of a structure. The effects of relative stiffness between super-structure and base material on dynamic soil-structure interaction are evaluated by parametric studies. Two 5-story buildings are used for the study; one is shearwall structure with relatively higher fundamental frequency and the other is frame structure with relatively lower fundamental frequency. The structures are modeled as beam-sticks coupled with springs and dashpots representing the base material. Dynamic equilibrium equations of the soil-structure interaction system are sloved by mode superposition method using Rosset modal damping values. Soil-structure interaction effect is found to be major concern in seismic analysis of shearwall structure in most cases while it seldom becomes engineering problem in frame-type structure. It is also found that seismic responses at lower elevation of the super-structure are amplified though they decrease at higher elevation as soil-structure interaction effects of the system increase.