• Korean Journal of Food Science and Technology
• /
• v.28 no.3
• /
• pp.411-416
• /
• 1996

Supercritical fluid extraction of ${\beta}$-carotene from carrot was optimized to maximize ${\beta}$-carotene (Y) extraction yield. A central composite design involving extraction pressure ($X_1$ 200-,100 bar), temperature ($X_2,\;35-51^{\circ}C$) and time ($X_1$$ 60-200min) was used. Three independent factors ($X_1,\;X_2,\;X_3$) were chosen to determine their effects on the various responses and the function was expressed in terms of a quadratic polynomial equation,$Y={\beta}_0+{\beta}_1X_1+{\beta}_2X_2+{\beta}_3X_3+{\beta}_11X_12+{\beta}_22X_3^2+{\beta}_-12X_1X_2+{\beta}_12X_1X_2+{\beta}_13X_1X_3+{\beta}_23X_2X_3,$ which measures the linear, quadratic and interaction effects. Extraction yields of ${\beta}$-carotene were affected by pressure, time and temperature in the decreasing order, and linear effect of tenter point (${\beta}_11$) and pressure (${\beta}_1$) were significant at a level of 0.001(${\alpha}$). Based on the analysis of variance, the model fitted for ${\beta}_11$-carotene (Y) was significant at 5% confidence level and the coefficient of determination was 0.938. According to the response surface of ${\beta}$-carotene by cannoical analysis, the stationary point for quantitatively dependent variable (Y) was found to be the maximum point for extraction yield. Response area for ${\beta}$-carotene (Y) in terms of interesting region was estimated over $10,611{\mu}g$ Per 100 g raw carrot under extraction.

• Korean Journal of Food Science and Technology
• /
• v.37 no.3
• /
• pp.431-437
• /
• 2005

Overall experiments were planned by central composite design, and results were analyzed by response surface methodology (RSM) to determine effects of three independent variables, temperature ($X_{1}$), extraction time ($X_{3}$), and pressure ($X_{3}$), on yield of sesame oil extract (Y). Regression equation model optimized by response surface analysis was: Y (sesame oil) = $-3.89+0.07X_{1}+0.03X_{2}+0.0006X_{3}-0.0007X_{1}^{2}-0.0002X_{2}X_{1}-0.00008X_{2}^{2}+0.000004X_{3}X_{1}+0.0000009X_{3}X_{2}-0.00000009X_{3}^{2}$. According to RSM analysis, optimum extracting conditions of temperature, time, and pressure were $45.89^{\circ}C$, 131.89 min, and 34228.41 kPa, respectively, and statistical maximum yield of sesame oil was 96.27%. Fatty acid composition of sesame oil showed sesame oil extracted by Supereritical Fluid $CO_{2}$ contained lower levels of palmitic, stcaric, and oleic acids and higher levels or palmitoleic and linoleic acids than commercial sesame oil. Commercial and extracted sesame oils were analyzed by electronic nose composed of 12 different metal oxide sensors. Obtained data were interpreted by statistical method of MANOVA. Sensitivities of sensors from electronic nose were analysed by principal component analysis. Proportion of first principal component was 99.92%. All sesame oils showed different odors (p < 0.05).

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.31 no.3
• /
• pp.417-422
• /
• 1998

This study was performed to obtain information about the extraction conditions of lipids from microalgae, Spirulina platensis, using supercritical fluid $CO_2$. Regardless of extraction temperature conditions, the extracted lipid contents increased as pressure increased, but decreased at 8500 psi on each temperature. The highest yield of extracted lipid content showed in the condition of 5500psi at $50^{\circ}C$, and extracted lipid content was about $20\%$. In same pressure the contents of C18 : 2 and C20 : 0 increased as temperature increased, but fatty acids composition were $60\~75\%$ saturated, $12\~20\%$ monounsaturated and $13\~31\%$ polyunsaturated regardless of extraction conditions. The C18 : 3 was only detected in the condition of 5500psi at $50^{\circ}C$ but the content was very little.

• Clean Technology
• /
• v.26 no.1
• /
• pp.1-6
• /
• 2020

Rapid expansion of supercritical solution (RESS) process was used to make molsidomine (MOL) loaded peracetyl-β-cyclodextrin (PAc-β-CD) nanoparticles, which were collected into the air. The effect of the concentration of the drug PAc-β-CD (0.5 and 1 wt%), extraction temperature (45 ~ 60 ℃), nozzle length (5 ~ 20 mm) and internal diameter (ID) (50 ~ 150 μm) of a capillary, and spray distance on the particle size and morphology of the resulting particles were investigated. The interaction of a drug and PAc-β-CD was confirmed by ¹H-NMR spectroscopy while the particle size was measured by means of a scanning electron microscope. It was found that increasing the temperature from 45 ℃ to 60 ℃ and decreasing the nozzle diameter from 150 μm to 50 μm had an increasing effect on the average particle size, while increasing the spray distance led to a decrease in the average particle size at a constant pressure of 34.5 MPa and temperature of 45 ℃. With 0.5 wt% of PAc-β-CD, the capillary nozzle of short length (5 mm) and small ID (50 μm) gave the smallest size (165 nm). The obtained nanoparticles showed increased dispersity and solubility in oil. The oil suspension of the inclusion complex showed increased sustainability, which can increase the in-vitro controlled release time of the drug.

• Biotechnology and Bioprocess Engineering:BBE
• /
• v.6 no.4
• /
• pp.212-230
• /
• 2001

Recent advances in recombinant DNA technology have resulted in development of many new protein drugs. Due to the unique properties of protein druges, they have to be delivered by parenteral injection Although delivery of protein drugs by other routes, such as pulmonary and nasal routes, has shown some promises, to date most protein drugs are administered by par-enteral routs. For long-term delivery of protein drugs by parenteral administration, they have been formulated into biodegradable microspheres. A number of microencapsulation methods have been developed, and the currently used microencapsulation methods are reviewed here, The microen-capsulation methods have been divided based on the method used. They are: solvent evapora-tion/extraction; phase separation (coacervation);spray drying; ionotropic gelation/polyelectrolyte complexation; interfacial polyumerization and supercritical fluid precipitation. Each method is de-scribed fro its applications, advantages, and limitations.

• 제어로봇시스템학회:학술대회논문집
• /
• 1990.10a
• /
• pp.246-251
• /
• 1990

A fuzzy control scheme has been proposed for a supercritical extraction process which has attracted much attention recently as a new separation technology. Based on the manual operation experience, three control pairs between manipulated and output variables are selected first and then seven membership functions are defined for control error and time rate of the error, respectively for each control pair, resulting in forty nine Fuzzy control rules. In addition to these, the membership functions are defined in two steps (coarse and fine) to enhance control performance. Fuzzy inference is performed using MAX-MTN composition rule and defuzzified control output is calculated based on center of gravity method. The prosed Fuzzy control scheme has been assessed through numerical simulation. As a result, the proposed scheme shows good control performance comparable with that by INA(inverse nyquist array) which usually requires complicated design procedure.

• Food Science and Preservation
• /
• v.20 no.6
• /
• pp.827-833
• /
• 2013

In this study we investigated physicochemical properties of Zanthoxylum schinifolium seeds oil base extracts. Supercritical fluid extraction (SFE), roast pressure (RPM) and steam pressure (SPM) method were used for oil base extracts. The pressure and temperature conditions of SFE method were $70{\sim}80kgf/cm^2$ and below $30^{\circ}C$, respectively, by newly designed SFE-$CO_2$ system. The yield of extraction was 38.5% at the SFE method and others were 30% in each. Refractive index of oil base extracts, there was also no difference between them as 1.470~1.473. At the SFE method, viscosity observed higher value better than two method that showed as 181.88~209.93 according to the extraction time. Three oil base extracts showed difference in color which was low in b value at SFE, especially. The result of acid value at RPM that was lower as 0.93 mg/g than 2.36~2.64 mg/g of SFE method. Saponification value ranged $182.96{\sim}196.57mg{\cdot}KOH/g$ in three extraction method. At SPM, TBA value showed as 158.96 mg/kg, but in the SFE method ranged higher value as 201.30~347.14 mg/kg. Fatty acids analysed with 18 varieties in all oil base extracts and the composition of saturated/unsaturated fatty acids was 17:83(v/v) at SEF. Especially, ${\omega}$-3,6,9 fatty acids observed at SFE and SPM, but did not appeared at RPM. Fatty acid of ${\omega}$-6,9 detected in all cases.

• Journal of Korea Technical Association of The Pulp and Paper Industry
• /
• v.47 no.6
• /
• pp.130-138
• /
• 2015

This study was conducted to investigate the effect of the strengthening treatment of methyl cellulose (MC) on properties and aging characteristics of the dewaxed papers during humid heating aging. Beeswax-treated Hanji was dewaxed by the supercritical fluid extraction method, and subsequently the strengthening treatment was performed with MCs having three different viscosities. MC was first applied by dipping a dewaxed paper into a MC solution whose concentration was controlled from 0.5% to 1.5%. After the strengthened papers were artificially aged at $80^{\circ}C$ and 65% RH, the changes in optical and mechanical properties of the samples were evaluated. The results show that viscosity and especially pick-up of MC influenced the strengthening efficiency and aging characteristics of dewaxed paper. Strength was increased with the MC coating weight; in addition, strengthening with MC improved preservability of the dewaxed paper. The optimum conditions for the strengthening with MC was found to be the coating ratio of 4% with 1500 cP MC.

• Korean Journal of Agricultural Science
• /
• v.43 no.2
• /
• pp.249-257
• /
• 2016

In this study, rapeseed extracts were obtained by supercritical carbon dioxide fluid extraction of defatted rapeseed to evaluate the stability and antioxidant activity of an oil-in-water (O/W) emulsion system. The oil-in-water emulsions were prepared from stripped soybean oil with different concentrations (0.3, 0.4, 0.5, and 0.6%) of rapeseed extract as an emulsifier. Their emulsion stability was compared to that of emulsions prepared with the commercial emulsifier, Tween 20 (Polysorbate 20, 0.2%). After stripping the soybean oil, the total tocopherol content was reduced from 51.4 g/100 g to 1.1 g/100 g. Emulsion stability and oxidative stability of emulsions prepared with Tween 20 and rapeseed extract as emulsifiers were evaluated. For 30 days droplet sizes of emulsions containing rapeseed extract (0.4, 0.5, and 0.6%) were not significantly different (p > 0.05). Similar results were obtained for emulsion stability (ES) and Turbiscan analysis, suggesting that the addition of rapeseed extract increased emulsion stability. The addition of rapeseed extract at more than 0.4% resulted in an emulsion stability comparable to the addition of 0.2% Tween 20. The antioxidative ability of rapeseed extract increased with the amount added in the emulsion. Moreover, the addition of 0.6% rapeseed extract resulted in the lowest emulsion peroxide values (10.3 mEq/L) among all treatments. Therefore, according to the stability of its antioxidative and physical stability properties, rapeseed extract from super critical extraction could be successfully applied to the food and cosmetic industries.

• Korean Journal of Food Science and Technology
• /
• v.31 no.6
• /
• pp.1465-1470
• /
• 1999

Aroma was extracted from Applemint(Mentha rotundifolia(L.) Huds) with SDE(simultaneous distillation and extraction), SFE(supercritical fluid extraction) and headspace method and the compounds of aroma were tentatively identified with GC-MS. The functionality of aroma compounds were determined with GC-olfactometry. Total 67 compounds were identified. Among them, 39 compounds were determined from SDE, 42 from SFE and 16 from headspace extract. Many terpene compounds were extracted with SDE and headspace methods but hydrocarbones with SFE. The major constituents of aroma obtained from SDE and SFE, were piperitenone oxide, germacrene-D and trans sabinene hydrate, but those from headspace method were 3-octanol, 1,8-cineol, camphene and benzeneacetaldehyde. Results of sniffing test, determining characteristics and strength of aroma showed that the major constituents of SDE extract were refreshing sweet and apple-like(ethyl-2-methyl butanoate), sweet and fruity-like$({\alpha}-thujene)$, fresh mushroom-like(1-octen-3-ol, 3-octanol), and bitter herb-like$({\delta}-cadidene)$. Major constituents of aroma extracts obtained from headspace method were alcoholic, refreshing sweet and apple-like(ethyl 2-methyl butanoate), unpleasant chemical, and bitter herb and grassy-like(camphene).