• Journal of Life Science
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• v.27 no.5
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• pp.561-566
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• 2017

Dendropanax morbifera Lev. is known in Korea for its golden sap and medicinal properties. The many biological activities of the leaf and stem extracts suggest that this tree could be a valuable source of medicinal compounds for the treatment of various ailments such as dermatitis, migraines, dysmenorrhea, muscle pain, and infectious diseases. However, there is little information on the composition and biological activity of the volatile fraction of D. morbifera. Therefore, in this study, the volatile compounds in leaves, stems, and sap of D. morbifera were isolated using solvent and supercritical fluid extraction (SFE), and analyzed by gas chromatography/mass spectrometry to reveal their chemical composition and identify potential compounds of interest. Fifteen compounds were identified in the leaf extracts, whereas 29 and 3 compounds were identified in the stem and sap extracts, respectively. The volatile profiles obtained using solvent and SFE differed. Esters and aromatic hydrocarbons predominated in the solvent extract of leaves and SFE extract of stems, whereas the solvent extract of stems and SFE extract of leaves contained terpenoids. Limonene, ${\alpha}$-pinene, and ${\beta}$-myrcene were identified in the volatile extract of sap, with limonene representing 96.30% of the total peak area. In addition, the antiproliferative effects of the solvent extracts of leaves and stems were evaluated, revealing that these solvent extracts were particularly effective in decreasing the proliferation of HepG2 cells.

• Korean Chemical Engineering Research
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• v.48 no.6
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• pp.747-751
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• 2010

The objective of this research were to produce amino acids from freezing-dried brown seaweed(Undaria pinnatifida) powder by sub- and supercritical water hydrolysis and to characterize the products. The hydrolysis was carried out in a batch type reactor consisting of 4.6 cm inside diameter and $200cm^3$ vessel and stir made of Hastelloy 276. A stir inside the reactor was continuously moving at 100 rpm. Brown seaweed powder and 100 mL of 1% acetic acid in distilled water were charged into the reactor at a ratio of 1:100(w/v). The applied conditions were $180{\sim}374^{\circ}C$, respectively for 1 hour. The total amino acid content was found to be significantly higher in brown seaweed hydrolyzed by low temperature comparing to high temperature. The amounts of low molecular weight amino acids(glycine, alanine, serine etc) were higher than that of high molecular weight amino acids. The maximum yields of amino acids were produced at low temperature($220^{\circ}C$) with acid catalyst.

• Journal of Ginseng Research
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• v.40 no.4
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• pp.423-430
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• 2016

Background: The induction of cellular defensive genes such as phase II detoxifying and antioxidant enzymes is a highly effective strategy for protection against carcinogenesis as well as slowing cancer development. Transcription factor Nrf2 (nuclear factor E2-related factor 2) is responsible for activation of phase II enzymes induced by natural chemopreventive compounds. Methods: Red ginseng oil (RGO) was extracted using a supercritical $CO_2$ extraction system and chemical profile of RGO was investigated by GC/MS. Effects of RGO on regulation of the Nrf2/antioxidant response element (ARE) pathway were determined by ARE-luciferase assay, western blotting, and confocal microscopy. Results: The predominant components of RGO were 9,12-octadecadienoic acid (31.48%), bicyclo[10.1.0] tridec-1-ene (22.54%), and 22,23-dihydrostigmasterol (16.90%). RGO treatment significantly increased nuclear translocation of Nrf2 as well as ARE reporter gene activity, leading to upregulation of heme oxygenase-1 and NAD(P)H:quinone oxidoreductase 1. Phosphorylation of the upstream kinases such as apoptosis signal-regulating kinase (ASK)1, mitogen-activated protein kinase (MAPK) kinase (MKK)4/7, c-Jun N-terminal kinase (JNK), and p38 MAPK were enhanced by treatment with RGO. In addition, RGO-mediated Nrf2 expression and nuclear translocation was attenuated by JNK inhibitor SP600125 and p38 MAPK inhibitor SB202190. Conclusion: RGO could be used as a potential chemopreventive agent, possibly by induction of Nrf2/ARE-mediated phase II enzymes via ASK1-MKK4/7-JNK and p38 MAPK signaling pathways.

• Korean Journal of Food Science and Technology
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• v.31 no.6
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• pp.1465-1470
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• 1999

Aroma was extracted from Applemint(Mentha rotundifolia(L.) Huds) with SDE(simultaneous distillation and extraction), SFE(supercritical fluid extraction) and headspace method and the compounds of aroma were tentatively identified with GC-MS. The functionality of aroma compounds were determined with GC-olfactometry. Total 67 compounds were identified. Among them, 39 compounds were determined from SDE, 42 from SFE and 16 from headspace extract. Many terpene compounds were extracted with SDE and headspace methods but hydrocarbones with SFE. The major constituents of aroma obtained from SDE and SFE, were piperitenone oxide, germacrene-D and trans sabinene hydrate, but those from headspace method were 3-octanol, 1,8-cineol, camphene and benzeneacetaldehyde. Results of sniffing test, determining characteristics and strength of aroma showed that the major constituents of SDE extract were refreshing sweet and apple-like(ethyl-2-methyl butanoate), sweet and fruity-like$({\alpha}-thujene)$, fresh mushroom-like(1-octen-3-ol, 3-octanol), and bitter herb-like$({\delta}-cadidene)$. Major constituents of aroma extracts obtained from headspace method were alcoholic, refreshing sweet and apple-like(ethyl 2-methyl butanoate), unpleasant chemical, and bitter herb and grassy-like(camphene).

• Korean Journal of Poultry Science
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• v.20 no.4
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• pp.217-231
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• 1993

Due to the increased awareness on the relationship between high cholesterol intake and cardiovascular disease, the development of low cholesterol eggs and egg products are necessary at the present time. Largely, two approaches are possible to develop low cholesterol eggs. The first approach is the production of low cholesterol eggs by altering feeds of layers, by administering drugs, by genetic selection of strains and breeds, and by management of laying cycle, age and egg size. The second approach is to manufacture low cholesterol eggs technically treated with adsorbant, solvent, enzyme, edible oil and supercritical fluid. Both approaches have their own pros and cons, respectively, as far as the cholesterol removal rates, the easeness of process, and the economic reasons are concerned. The low cholesterol egg production is quite labor-intensive and has relatively low cholesterol removal rate(30∼50％ ) compared to that of chemically treated eggs. On the other hand, the low cholesterol eggs treated with chemicals have a very high cholesterol removal rate (80∼90％) but some of the methods are not completely safe as food processing purposes due to the residual chemicals and the sensory quality is not as good as the low cholesterol eggs without chemical treatment. Therefore, further studies must be conducted to improve the sensory quality of low cholesterol eggs and to eliminate fear about the harmfulness using low cholesterol eggs.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.9 no.3
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• pp.574-579
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• 2008

The evaporation heat transfer coefficient of $CO_2$ (R-744) in a horizontal tube was investigated experimentally. The main components of the refrigerant loop are a receiver, a variable-speed pump, a mass flow meter, a pre-heater and evaporator (test section). The test section consists of a smooth, horizontal stainless steel tube of inner diameter of 4.57mm. The experiments were conducted at mass flux of 400 to $900kg/m^2s$, saturation temperature of 5 to $20^{\circ}C$, and heat flux of 10 to $40kW/m^2$. The test results showed the heat transfer of $CO_2$ has a greater effect on nucleate boiling more than convective boiling. Mass flux of $CO_2$ does not affect nucleate boiling too much. In comparison with test results and existing correlations, All of the existing correlations for the heat transfer coefficient underestimated the experimental data. However Jung et al.'s correlation showed a good agreement with the experimental data. Therefore, it is necessary to develope accurate predictions determining the evaporation heat transfer coefficient of $CO_2$ in horizontal tubes.

• Journal of the Korean Wood Science and Technology
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• v.41 no.6
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• pp.583-593
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• 2013

The based-catalyzed depolymerization (BCD) of kraft lignin isolated from black liquor which the chemical pulping of a mixture of various Southeast Asia hardwood chips was carried out in a batch reactor in the presence of different NaOH concentrations with supercritical methanol. The S:G ratio of the kraft lignin determined by pyrolysis-GC/MS analysis turned out roughly 1.4:1 and main products were vanillic acid, syringol and 3-methoxy catechol. The diethyl ether extracts as phenolic monomers from BCD reaction were produced similar yield among different NaOH concentrations. The 21 compounds were identified by GC/MS analysis in all experiments and major products were catechol, 3-methoxycatechol, 4-methylcatechol, syringol and isovanillic acid. However, it had been shown to be different monomer contents depending on the dosage of NaOH. Catechol, 4-methylcatechol and 3-methoxycatechol were shown to be the dominant monomer from BCD reaction using 7.5 and 3.25% of NaOH concentration whereas syringol, isovanillic acid, 3-methoxycatechol and 4-methylcatechol were determined to be the most typical products under the condition of 1.63% NaOH.

• Journal of the Korean Institute of Gas
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• v.25 no.4
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• pp.10-18
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• 2021

CO₂ storage technology in an aquifer is one of the most effective way to decrease global warming due to a high storage capacity and economics. A demonstration-scale offshore CO₂ storage project was performed in a geological deep aquifer in the Pohang Basin, Korea for a technological development of large-scale CO₂ storage. A challenging issue in the early design stage of the project was to establish the proper injectivity during CO₂ injection. To solve this issue, injection conditions were calculated by calculating injection rate, pressure, temperature, CO₂ phase change, and thermodynamic properties. For this study, we simulated and numerically analyzed CO₂ phase change from gas to supercritical phase and flow behavior in transport piping and injection tubing using OLGA program. Our results provide the injectivity conditions of CO₂ injection system combined with a bottomhole pressure of an aquifer.

• Korean Journal of Environmental Biology
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• v.36 no.4
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• pp.659-664
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• 2018

In this study, two essential oils (EOs) extracted from Aleriana fauriei and Alpinia galangal were formulated as an emulsifiable concentrate (EC) and a granule. In the evaluation of their acute toxicity on fishes, Cyprinus carpio adults were used and the toxicities were determined in a static condition. The formulations were prepared from the essential oil extracted by three different methods namely steam distillation (SD), solvent extraction (SE) and supercritical fluid extraction (SFE). The acute toxicities were calculated using $LC_{50}$ values. Among EOs, only the EO extracted by solvent showed acute toxicities on carps. Some of the EC, EOs of Aleriana fauriei did not exhibit toxicity, while EOs from Alpinia galangal showed potent acute toxicities on carps. Among the granules, granules formulated with Aleriana fauriei EO extracted by SD method and Alpinia galangal EO extracted by SFE method showed acute toxicities on fishes. Nevertheless, $LC_{50}$ of ECs and granules formulated with all types of EOs in this study was higher than the fish toxicity level III for pesticides suggested by Korea Rural Development Administration. Furthermore, cytochrome P450 1A and glutathione S-transferase were confirmed as biomarkers in carps in response to the exposure to Alpinia galangal EO extracted by SD and SFE method, tracking Alpinia galangal EO in the aquatic environment.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.45 no.3
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• pp.319-331
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• 2019

This study was conducted to find a solvent, a extraction method and optimized conditions for Artemisia iwayomogi extract. which is effective to anti-inflammatory and antioxidant activity. A. iwayomogi extract by distilled water, ethanol, methanol, hexane and ethyl acetate using ultrasonic, high-pressure and supercritical extraction was investigated for NO inhibitory and DPPH radical scavenging activity. Extract obtained by ethanol and ultrasonic extraction method showed high NO production inhibitory activity, DPPH free radical scavenging activity and yield. Response surface methodology (RSM) was applied to find a optimized ultrasonic extraction conditions. Results showed that the optimum conditions for the higher yield were ethanol solvent of 45.71% concentration with extraction time and ultrasonic power of 63.33min and 308.84 W, respectively. This condition predicted 15.85% yield, but real yield was $16.40{\pm}0.28%$. The optimum conditions for simultaneous anti-inflammatory and antioxidant activity were established as ethanol concentration (80.81%), extraction time (90.00 min) and ultrasonic power (400.00 W). NO production inhibitory and antioxidant activity were $89.77{\pm}1.37%$ and $60.12{\pm}0.39%$, respectively. These results showed similar to the predicted values of 94.54%, 58.03% respectively.