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Study of Optimized Extraction Conditions for Simultaneous Anti-inflammatory and Antioxidant Activity of Artemisia iwayomogi using Response Surface Methodology

반응표면분석을 이용한 한인진의 항염 및 항산화 복합 활성 최적 추출 조건에 관한 연구

  • 박다원 (한국화학융합시험연구원) ;
  • 최우석 (한국화학융합시험연구원) ;
  • 이창현 (한국화학융합시험연구원)
  • Received : 2019.09.04
  • Accepted : 2019.09.27
  • Published : 2019.09.30

Abstract

This study was conducted to find a solvent, a extraction method and optimized conditions for Artemisia iwayomogi extract. which is effective to anti-inflammatory and antioxidant activity. A. iwayomogi extract by distilled water, ethanol, methanol, hexane and ethyl acetate using ultrasonic, high-pressure and supercritical extraction was investigated for NO inhibitory and DPPH radical scavenging activity. Extract obtained by ethanol and ultrasonic extraction method showed high NO production inhibitory activity, DPPH free radical scavenging activity and yield. Response surface methodology (RSM) was applied to find a optimized ultrasonic extraction conditions. Results showed that the optimum conditions for the higher yield were ethanol solvent of 45.71% concentration with extraction time and ultrasonic power of 63.33min and 308.84 W, respectively. This condition predicted 15.85% yield, but real yield was $16.40{\pm}0.28%$. The optimum conditions for simultaneous anti-inflammatory and antioxidant activity were established as ethanol concentration (80.81%), extraction time (90.00 min) and ultrasonic power (400.00 W). NO production inhibitory and antioxidant activity were $89.77{\pm}1.37%$ and $60.12{\pm}0.39%$, respectively. These results showed similar to the predicted values of 94.54%, 58.03% respectively.

본 연구에서는 한인진으로부터 항염 및 항산화 활성이 뛰어난 추출물을 추출하기 위한 용매 및 추출법을 개발하여 최적의 추출 조건을 확인하였다. 증류수, 에탄올, 메탄올, 헥산 및 EA를 추출 용매로 한 초음파, 초고압 추출법과 초임계 추출법으로 추출을 한 후 NO 생성 저해 활성과 DPPH free radical 소거 활성을 비교 확인하였다. 그 결과 항염, 항산화 활성, 수율 및 안전성을 모두 고려하여 볼 때 에탄올 용매의 초음파 추출법이 가장 좋은 결과를 보였다. 이를 바탕으로 초음파 최적 추출 조건을 설정하기 위해 반응표면분석법(RSM)을 사용하여 연구한 결과, 수율의 최적 추출 조건은 에탄올 함량(45.71%), 추출 시간(63.33 min), 초음파 출력량(308.84 W)일 때, 15.85%의 가장 높은 수율을 보이는 것으로 예측되었고 실제 수율은 $16.40{\pm}0.28%$였다. 항염과 항산화 복합 활성의 최적 추출 조건은 에탄올 함량(94.54%), 추출 시간(90.00 min), 초음파 출력량(400.00 W)일 때, 각각 94.54%, 58.03%로 가장 높은 값을 보이는 것으로 예측되었고 실측검증에서 각각 $89.77{\pm}1.37%$, $60.12{\pm}0.39%$으로 예측 값과 유사한 결과를 보였다.

Keywords

References

  1. R. K. Bijauliya, S. Alok, M. Kumar, D. K. Chanchal, and S. Yadav, A comprehensive review on herbal cosmetics, Int J Pharm Sci Res, 8(12), 4930 (2017).
  2. E. S. Sohn, S. W. Kim, J. S. Kang,and S. P. Lee, Technology trend and patent information analysis of cosmetic materials derived from natural products, Applied Chemistry, 8(2), 466 (2004).
  3. Y. E. Song, J. S. Ryu, J. R. Chung, J. S. Kwak, D. H. Kim, B. S. Kim, and C. W. Rim, Study on the biological activity of Artemisia iwayomogi KITAMURA, Korean J. Medicinal Crop Sci., 9(2), 116 (2001).
  4. H. K. Han, Effect of Artemisia iwayomogi ethanol extract on hypoglycemic and antioxidant activities in diabetic rats, J Korean Soc Food Sci Nutr, 41(12), 1716 (2012). https://doi.org/10.3746/jkfn.2012.41.12.1716
  5. S. M. Nam, S. S. Ham, D. H. Oh, I.J. Kang, S. Y. Lee, and C. K. Chung, Effects of Artemisia iwayomogi Kitamura ethanol extract on lowering serum and liver lipids in rats, J Korean Soc Food Sci Nutr, 27(2), 338 (1998).
  6. E. J. Park, J. X. Nan, J. Y. Kim, H. C. Kang, J. H. Choi, S. J Lee, B. H. Lee, S. J. Kim, J. H. Lee, Y. C. Kim, and D. H. Sohn, The ethanol-soluble part of a hot-water extract from Artemisia iwayomogi inhibits liver fibrosis induced by carbon tetrachloride in rats, J. Pharm. Pharmacol., 52(7), 875 (2000). https://doi.org/10.1211/0022357001774561
  7. H. Ahn, J. Y. Kim, H. J. Lee, Y. K. Kim, and J. H. Ryu, Inhibitors of inducible nitric oxide synthase expression from Artemisia iwayomogi, Arch. Pharm. Res., 26(4), 301 (2003). https://doi.org/10.1007/BF02976959
  8. A. R. Kim, Y. N. Zou, T. H. Park, K. H. Shim, M. S. Kim, N. D. Kim, J. D. Lim, S. J. Bae, J. S. Choi, and H. Y. Chung, Active components from Artemisia iwayomogi displaying $ONOO^{-}$ scavenging activity, Phytother Res, 18(1), 1 (2004). https://doi.org/10.1002/ptr.1358
  9. N. Y. Kim, H. J. Koh, H. Li, H. J. Lee, and J. H. Ryu, Inhibitory effect of a sesquiterpene from Artemisia iwayomogi on expression of inducible nitric oxide synthase by suppression of I-${\kappa}B{\alpha}$ degradation in LPS-stimulated RAW 264.7 cells, Nat Prod Sci., 23(2), 92 (2017). https://doi.org/10.20307/nps.2017.23.2.92
  10. J. H. Ryu, H. Ahn, J. Y. Kim, and Y. K. Kim, Inhibitory activity of plant extracts on nitric oxide synthesis in LPS-activated macrophages, Phytother Res, 17(5), 485 (2003). https://doi.org/10.1002/ptr.1180
  11. D. Yan, J. A. Jeong, S. Y. Yang, W. K. Kim, S. H. Lee, H. D. Jang, and Y. H. Kim, Antioxidative sesquiterpenes from Artemisia iwayomogi, Bull. Korean Chem. Soc., 32(9), 3493 (2011). https://doi.org/10.5012/bkcs.2011.32.9.3493
  12. H. R. Lee, B. R. Jung, J. Y. Park, I. W. Hwang, S. K. Kim, J. U. Choi, S. H. Lee, and S. K. Chung, Antioxidant activity and total phenolic contents of grape juice products in the Korean market, Korean J. Food Preserv., 15(3), 445 (2008).
  13. D. O. Moon, Y. H. Choi, N. D. Kim, Y. M. Park, and G. Y. Kim, Anti-inflammatory effects of ${\beta}$-lapachone in lipopolysaccharide-stimulated BV2 microglia, Int. Immunopharmacology., 7(4), 506 (2007). https://doi.org/10.1016/j.intimp.2006.12.006
  14. N. J. Rothwell and G. N. Luheshi, Interleukin 1 in the brain: biology, pathology and therapeutic target, Trends Neurosci., 23(12), 618 (2000). https://doi.org/10.1016/S0166-2236(00)01661-1
  15. R. Korhonen, A. Lahti, H. Kankaanranta and E. Moilanen, Nitric oxide production and signaling in inflammation, Current Drug Targets-Inflammation &Allergy, 4(4), 471 (2005). https://doi.org/10.2174/1568010054526359
  16. S. M. Lucas, N. J. Rothwell, and R. M. Gibson, The role of inflammation in CNS injury and disease, Br. J. Pharmacol., 147(S1), S232 (2006). https://doi.org/10.1038/sj.bjp.0706400
  17. J. H. Woo, S. L. Shin, Y. D. Chang, and C. H. Lee, Antioxidant Effect according to Extraction Method in Extracts of Dendranthema zawadskii var. yezoense and Cosmos bipinnatus, Kor. J. Hort. Sci. Technol., 28(3), 462 (2010).
  18. H. M. Kim, Y. Y. Lim, S. M. Cho, M. Y. Kim, I. P. Son, J. M. Suk, J. O. Park, J. H. Park, J. W. Cho, and B. J. Kim, The evaluation of skin safety and skin cell toxicity for Scutellaria baicalensis Georgi extract according to extraction conditions, Korean J Dermatol, 50(11), 959 (2012).
  19. E. Y. Kim, I. H. Baik, J. H. Kim, S. R. Kim, and M. R. Ryu, Screening of the antioxidant activity of some medicinal plants, Korean J. Food Sci. Technol., 36(2), 333 (2004).
  20. L. Jin, J. H. Ha, Y. Y. Choi, Y. C. Seo, J. S. Kim, Y. O. Kim, S. W. Cha, J. C. Kim, and H. Y. Lee, Enhancement of cosmeceutical activites of Berberis koreana bark by ultra-high pressure and ultrasonification extraction process, Korean J. Medicinal Crop Sci., 19(1), 54 (2011). https://doi.org/10.7783/KJMCS.2011.19.1.054
  21. J. H. Kim, D. H. Kim, J. H. You, C. H. Kim, M. C. Kwon, N. S. Seong, S. E. Lee, and H. Y. Lee, Immuno-regulatory activities of various fractions from Ehpedrae Sinica STAPF, Rubus Coreanus Miq. and Angelica gigas Nakai extracts with ultrasonification, Korean J. Medicinal Crop Sci., 13(4), 161 (2005).
  22. Z. Shouqin, Z. Junjie, and W. Changzhen, Novel high pressure extraction technology, Int J Pharm, 278(2), 471 (2004). https://doi.org/10.1016/j.ijpharm.2004.02.029
  23. E. J. Lee, S. A. Yang, H. D. Choi, H. G. Im, K. Whang, and I. S. Lee, Comparison of gingerols in various fractions and the antioxidant effects of supercritical fluid extracts from ginger, Korean J. Food Sci. Technol., 43(4), 469 (2011). https://doi.org/10.9721/KJFST.2011.43.4.469
  24. D. Ryu and E. Koh, Optimization of ultrasound-assisted extraction of anthocyanins and phenolic compounds from campbell early grape using response surface methodology, Korean J. Food Sci. Technol., 50(5), 474 (2018). https://doi.org/10.9721/KJFST.2018.50.5.474
  25. J. R. Kang, S. J. Lee, C. R. Hwang, J. H. Shin, M. J. Kang, and N. J. Sung, Optimization of extraction conditions for mixing beverage development of black garlic and Gaeddongssuk by response surface methodology, Journal of Agriculture & Life Science, 46(2), 139 (2012).
  26. K. E. Oh, H. Shin, Y. H. Jeon, Y. H. Jo, M. K. Lee, K. S. Lee, B. Park, and K. Y. Lee, Optimization of pancreatic lipase inhibitory and antioxidant activities of Ilex paraguariensis by using response surface methodology, Arch. Pharm. Res., 39(7), 946 (2016). https://doi.org/10.1007/s12272-016-0768-y
  27. K. Thaipong, U. Boonprakob, K. Crosby, L. Cisneros-Zevallos and D. H. Byrne, Comparison of ABTS, DPPH, FRAP, and ORAC assays for estimating antioxidant activity from guava fruit extracts, Journal of Food Composition and Analysis, 19(6-7), 669 (2006). https://doi.org/10.1016/j.jfca.2006.01.003
  28. V. Dewanto, X. Wu, K. K. Adom, and R. H. Liu, Thermal processing enhances the nutritional value of tomatoes by increasing total antioxidant activity, J. Agric. Food Chem., 50(10), 3010 (2002). https://doi.org/10.1021/jf0115589
  29. D. Mladenova and M. R. Kohonen-Corish, Mouse models of inflammatory bowel disease-insights into the mechanisms of inflammation-associated colorectal cancer, In vivo, 26(4), 627 (2012).
  30. C. Nathan and Q. W. Xie, Regulation of biosynthesis of nitric oxide, J. Biol. Chem., 269(19), 13725 (1994). https://doi.org/10.1016/S0021-9258(17)36703-0
  31. D. Noh, J. G. Choi, S. S. Hong, and M. S. Oh, Comparison of anti-inflammatory effects between Artemisia capillaris and Artemisia iwayomogi by extraction solvents, Kor. J. Herbol, 33(3), 55 (2018). https://doi.org/10.6116/KJH.2018.33.3.55
  32. S. B. Kedare and R. P. Singh, Genesis and development of DPPH method of antioxidant assay, J. Food Sci Technol, 48(4), 412 (2011). https://doi.org/10.1007/s13197-011-0251-1
  33. J. I. Kim, S. W. Park, J. J. Lim, S. I. Sohn, J. S. Shin, S. C. Park, Y. P. Jang, E. K. Chung, H. W. Lee, and K. T. Lee, Gastroprotective effects of the isopropanol extract of Artemisia princeps and its gastroretentive floating tablets on gastric mucosal injury, Acta Pharm, 67(4), 479 (2017). https://doi.org/10.1515/acph-2017-0037
  34. M. H. Bang, D. H. Kim, J. S. Yoo, D. Y. Lee, M. C. Song, H. J. Yang, T. S. Jeong, K. T. Lee, M. S. Choi, H. G. Chung, and N. I. Baek, Development of biologically active compounds from edible plant sources X IV. Isolation and identification of flavonoids from the aerial parts of Sajabalssuk (Artemisia herba), J. Korean Soc. Appl. Biol. Chem., 48(4), 418 (2005).
  35. B. Prakash, R. Shukla, P. Singh, A. Kumar, P. K. Mishra, and N. K. Dubey, Efficacy of chemically characterized Piper betle L. essential oil against fungal and aflatoxin contamination of some edible commodities and its antioxidant activity, Int. J. Food Microbiol., 142(1-2), 114 (2010). https://doi.org/10.1016/j.ijfoodmicro.2010.06.011