• Title/Summary/Keyword: sucrose content

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Mass Proliferation of Hibiscus hamabo Adventitious Root in an Air-lift Bioreactor, and the Antioxidant and Whitening Activity of the Extract (생물반응기를 이용한 황근 부정근의 대량증식과 추출물의 항산화 및 미백 활성 평가)

  • Lee, Jong-Du;Hyun, Ho Bong;Hyeon, Hyejin;Jang, Eunbi;Ko, Min-Hee;Yoon, Weon-Jong;Ham, Young Min;Jung, Yong-Hwan;Choi, Hwon;O, Eu Gene;Oh, Daeju
    • Korean Journal of Plant Resources
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    • v.35 no.4
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    • pp.435-444
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    • 2022
  • Hibiscus hamabo Sieb. et Zucc. (yellow hibiscus) is a deciduous semi-shrub plant and mainly growing in Jeju Island. This is known the unique wild hibiscus genus and classified as an 2nd grade of endangered plant for Korean Red List. In previous studies, properties of germination, ecological, genetical and salt resistance have been reported. In this study, we investigated mass-proliferated adventitious root using bioreactor, antioxidant and whitening effects to conduct functional ingredients. Yellow hibiscus were collected from Gujwa, Jeju by prior permission and they were introduced by explant type and various medium composition after surface sterilization. As a result, seed response rates were evaluated at range of 51.17~51.83%, in terms of comprehensive efficiency of shoot and root formation. In the case of adventitious root propagation condition was confirmed in half strength Murashige and Skoog medium salts, 30 mg/L sucrose, and 2 mg/L indole-3-butyric acid for 8 weeks in 5,000 mL bioreactor. We also compared between relationship with biomass and secondary metabolites accumulation by total phenolics content, the flavonoid content, DPPH free radical scavenging activity and melanin content. The results indicated that adventitious root mass proliferation, antioxidant and whitening effect could develop value of the high-quality cosmeceutical ingredient and further metabolite studies.

Component Analysis and Antioxidant Activity of Opuntia ficus-indica var. saboten (손바닥 선인장 열매의 영양성분 분석과 항산화 활성)

  • Shin, Eon-Hwan;Park, Sung-Jin;Choi, Sang-Kyu
    • Journal of the East Asian Society of Dietary Life
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    • v.21 no.5
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    • pp.691-697
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    • 2011
  • The purpose of this study was to determine the possibility of using Opuntia ficus-indica as a natural health food source. To accomplish this, the contents of general and antioxidative nutrient contents of Opuntia ficus-indica were measured. The carbohydrate, crude protein, crude fat and crude ash were 66.79%, 5.51%, 9.89% and 9.29%, respectively. The calorie contents of Opuntia ficusindica was 378.21 kcal. The content of total dietary fiber was 36.54%. The essential and non-essential amino acids contents were 1,635.14 mg and 3,012.68 mg, respectively. Potassium was the most abundant mineral followed by Ca, Mg, and Na, showing that Opuntia ficus-indica is an alkali material. The electron-donating activity (EDA) of Opuntia ficus-indica was 29.85~44.57%, and the activity was dependent on the sample concentration. Total phenolic content of Opuntia ficus-indica was 2.21 ${\mu}g$/mg, and total flavonoids content was estimated as 1.80 ${\mu}g$/mg. Opuntia ficus-indica extract showed the highest reducing power (OD 700=3.18) at a concentration of 6.25 mg/mL. Based on the above results, we determined that the Opuntia ficus-indica has potential antioxidant activities.

Quality Characteristics of Sweet Potato Varieties Baked and Freeze Thawed (원적외선으로 구운 군고구마의 품종별 냉·해동 후 품질 특성)

  • Jang, Gwi Yeong;Li, Meishan;Lee, Sang Hoon;Woo, Koan Sik;Sin, Hyun Man;Kim, Hong Sig;Lee, Junsoo;Jeong, Heon Sang
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.42 no.3
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    • pp.403-409
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    • 2013
  • We investigated the quality characteristics of sweet potatoes [Shinyulmi (SM), Yeonhwangmi (HM) and Yunmi (YM) variety] baked and subsequently freeze thawed. Baking was performed with a far infrared radiation oven at $250^{\circ}C$ for 40 minutes. Baked sweet potatoes were frozen at $-18^{\circ}C$ for 30 days, and thawed by a microwave oven for 3 minutes. The crude fat and protein content of HM were higher than the SM and YM varieties. Total sugar, reducing sugar, and free sugar content were increased after baking. Lightness, redness, and yellowness, as well as content of ${\beta}$-carotene, decreased after baking and freeze-thawing. Hardness, gumminess, and cohesiveness decreased during thawing after freezing, except for the YM variety. In a sensory evaluation, appearance, flavor, sweetness and overall acceptance were higher from baking and freeze-thawing the SM variety, instead of the HM and YM variety. From the results, the SM variety is preferred for the production of baked sweet potatoes.

Analysis of Nutrition and Antioxidants of Yak-Kong Chungkukjang Powder Added Black Foods (블랙푸드가 첨가된 약콩청국장분말의 영양소 및 생리활성물질 분석)

  • Kong, Hyun-Joo;Park, Heyun-Sook;Kim, Tae-Hoon;Shin, Seung-Ryeul;Hong, Ju-Yeon;Yang, Kyung-Mi
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.42 no.11
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    • pp.1727-1735
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    • 2013
  • This study is carried out to investigate the analysis of nutrition and antioxidants of soybean Chungkukjang powder (SCP), Yak-Kong Chungkukjang powder (YCP) and Yak-Kong Chungkukjang powder added black foods (YCBP) for the management of Alzheimer's disease. The water content of YCBP was higher than that of SCP and YCP. The soluble and crude protein contents of YCP were the highest among three Chungkukjang powders. In SCP, YCP and YCBP, the ratios of unsaturated and saturated fatty acids were 5.17, 5.76, and 5.78, respectively. The mineral content of SCP was higher than that of YCP and YCBP. Antioxidants analysis showed that the content of diadzein was the highest in YCP and genistein, and anthocyanin contents were higher than the others in YCBP. In conclusion, YCP and YCBP had higher contents than SCP in soluble and crude proteins, and unsaturated fatty acids, which are needed for composition and function of the brain tissue. Also, it was found that the contents of diadzein, genistein and anthocyanin with outstanding antioxidative ability were high. Thus, this study suggests that Chungkukjang powder, which is made with Yak-Kong, black sesame, black rice and sea tangle, can be utilized for in vivo experiment for the control of oxidative stress, reported as cause and therapy for Alzheimer's Disease.

Effects of Drainage Depths on Agronomic Characteristics, Yield and Feed Value of Silage Corn Hybrid in Paddy Field of Lowland (저지대 논 토양에서 배수로 깊이가 사일리지용 옥수수의 생육특성 및 사료가치에 미치는 영향)

  • Lee, Sang Moo
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.35 no.2
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    • pp.137-144
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    • 2015
  • This study was carried out to investigate the growth characteristics, yield, and chemical compositions of corn hybrids for silage according to the drainage depths in a lowland paddy field. The experimental design was arranged in a randomized block design with four treatments and three replications. The drainage depths of the four treatments were 0 cm, 20 cm, 30 cm and 50 cm. Tip-filling degree, ear length, and ear circle were higher in the 30 cm and 50 cm treatments compared with the other treatments (p < 0.05); but the plant length, leaf length, leaf width, ear height, stem diameter, and stem hardness were not significantly different (p < 0.05). The brix was higher in the order of 20 cm > 0 cm > 50 cm > 30 cm treatment. The fresh yield, dry matter yield, and TDN yield increased as the drainage depth increased (p < 0.05). The crude-protein and crude-ash content were highest in the 50 cm treatment (p < 0.05), but the crude-fat, NDF, ADF, and crude-fiber levels were not significantly different (p < 0.05). The total mineral content was higher in the order of 0 cm > 30 cm > 20 cm > 50 cm treatment (p < 0.05). Fructose, glucose, and sucrose were highest in the 20 cm treatment. The total amino-acid (EAA + NEAA) content was the highest in the 50 cm treatment. Based on these results, greater drainage depths increased the yield and feed value of silage corn.

Changes in Chemical Components and Antioxidant Activity of Dried Jujube with Different Aging Temperatures and Durations (가온숙성온도와 기간에 따른 건대추의 성분과 항산화활성 변화)

  • Park, Hye-Jin;Lee, Sang-Hoon;Kim, Hyun-Young;Jang, Gwi-Yeong;Hwang, In-Guk;Woo, Koan-Sik;Kwon, Oh-Sung;Lee, Jun-Soo;Jeong, Heon-Sang
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.41 no.5
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    • pp.591-597
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    • 2012
  • This study was conducted to investigate the changes in chemical components and antioxidant activities of dried jujube ($Zizyphus$ $jujuba$ Miller) with different aging temperatures ($60^{\circ}C$, $70^{\circ}C$, and $80^{\circ}C$) and durations (5 days, 10 days, 15 days, 30 days, and 50 days). The browning index increased from 0.124 to 1.998 with increased aging temperature and duration. As aging duration increased, fructose, glucose, and sucrose content decreased from 24.96% to 4.96%, from 22.23% to 4.41%, and from 18.41% to 0%, respectively. Reducing sugar content decreased from 351.52 mg/g to 155.96 mg/g as aging temperature and duration increased. Total acidity slightly increased from 1.61 to 3.13%. 5-Hydroxymethyl-2-furaldehyde increased from 0.002 mg/g to 38.84 mg/g, respectively. As aging temperature and duration increased, total polyphenol content increased from 15.98 mg/g to 38.61 mg/g. DPPH and ABTS radical scavenging activities increased from 4.03 mg trolox eq/g to 22.22 mg trolox eq/g, and from 2.11 mg ascorbic acid eq/g to 13.39 mg ascorbic acid eq/g, respectively. Reducing power increased rapidly in the first stage and then slowly increased afterward.

Effect of Salting Levels on the Changes of Taste Constituents of Domestic Fermented Flounder Sikhae of Hamkyeng-Do (함경도 지방의 전통가자미식해의 소금 첨가수준에 따른 숙성중 맛 성분의 변화에 관한 연구)

  • Jung, Hae-Suk;Lee, Su-Hak;Woo, Kang-Lyung
    • Korean Journal of Food Science and Technology
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    • v.24 no.1
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    • pp.59-64
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    • 1992
  • Salted and then washed flounder muscles with salting levels of 10%, 15% and 20% were mixed with boiled foxtail millet and spices(radish, garlic, ginger and red pepper) and fermented at $15^{\circ}C$ for 7 days. The changes of taste constituents of fermented flounder Sikhae, such as sugars, free amino acids and 5'-nucleotides, were investigated. The content of fructose decreased significantly during Sikhae fermentation, but the content of mannitol that was not detected from raw material was estimated to be $6.26%{\sim}8.97%$ in Sikhae. The content of total free amino nitrogen in the 15% salted Sikhae was 290.6 mg% and the highest value with 53.4% of its extract nitrogen. It is believed that leucine, alanine, arginine, glutamine, isoleucine, valine, glutamic acid and lysine may play an important role as the taste constituents in Sikhae. The detected 5'-nucleotides were CMP, UMP, CTP, AMP, ADP and ATP and among them the nucleotide showing the hightest level irrespective of treatment was UMP estimated to be $761.0\;{\mu}g{\sim}849.0\;{\mu}g/g$. ATP and ADP were significantly decreased in Sikhae, but CMP and CTP were significantly increased in the 15% salted Sikhae compared with those of raw material.

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Use of East Deep Sea Water for the Increase of Functional Components of Ginseng (Panax ginseng C.A. Meyer) and Tomato (Lycopersicon eculentum L.) (인삼과 토마토의 기능성 성분 증진을 위한 동해 해양심층수의 이용)

  • Woo Cheon-Seok;Kang Won-Hee
    • Korean Journal of Plant Resources
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    • v.19 no.2
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    • pp.331-335
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    • 2006
  • This experiment was conducted to investigate the effect of deep sea water on fruit quality and yield of tomato. In the deep sea water treatments, fruit growth and weight were decreased as the concentration of deep sea water increased. Especially, the fresh weight of second truss was decreased significantly than first truss. Soluble solid content was increased significantly in higher concentration treatment especially at 30mM and 40mM treatment. That was increased more in the first than in the second truss fruits. Most of hexose in fruits were glucose and fructose. The reason of increased glucose and fructose contents was the decline of growth because of salinity stress by deep sea water treatment. however deep sea water treatment increased the lycopene content, especially in 20mM treatment. It is assumed that deep sea water treatment cause induction and promotion of ethylene. The higher concentration of deep sea water to the solution, the eater fruit quality improvement was noticed. However, proportional yield reduction accompanied concentration, 20mM deep sea water improved fruit quality without a significant yield reduction. The Re content was the highest among ginsenosides in all treatments. The contents total of ginsenosides in all treatments, except EC 8 treatment, was higher than those in the controlled treatment. The PT/PD value was 1.31 of the lowest in the EC 8 treatment and was 2.52 of the highest in the EC6 treatment. Rf contents in high increase was detected at all treated ginseng roots.

Fermentation Kinetics for Production of Carotenoids by ${\beta}$-ionone Resistant Mutant of Xanthophyllomyces dendrorhous (Xanthophyllomyces dendrorhous 변이군주에 의한 Carotenoids 생산 발효의 특성 연구)

  • Park, Ki-Moon;Kim, Young-Jun;Song, Min-Woo;Kang, Seog-Jin;Lee, Jae-Heung
    • KSBB Journal
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    • v.21 no.4
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    • pp.286-291
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    • 2006
  • Various ${\beta}$-ionone resistant mutants were isolated from the wild-type red yeast Xanthophyllomyces dendrorhous KCTC 7704. Although the growth of X. dendrorhous KCTC 7704 was strongly inhibited at 0.025 mM ${\beta}$-ionone, one of the ${\beta}$-ionone resistant mutants isolated at 0.1 mM ${\beta}$-ionone by NTG mutagenesis showed rather 70% of relative survival at 0.15 mM ${\beta}$-ionone. Fermentation kinetics study with the mutant was carried out at $20^{\circ}C$ for 4 days in 300-mL baffled flasks. The mutant yielded up to 2.3-fold higher carotenoids content(viz. $1.2{\mu}g$ of total carotenoids per mg of dry cells) compared with the wild-type strain. The production of metabolites such as organic acids could be neglected. Studies on the kinetics with various carbon substrates revealed both an increase in final dry cell mass and a higher total carotenoids content in cell mass with glucose when compared to fructose or sucrose. As a further part of study, the effect of pH on the fermentation kinetics was investigated in glucose-limited chemostat at a dilution rate of $0.04h^{-1}$. When compared to steady-state kinetic parameters obtained at pH 4.0, a significant reduction in cell concentration at pH 3.0 and a lower carotenoids content at pH 5.2 were evident.

Physicochemical Properties and Sensory Evaluation of Citrus Unshiu Produced in Cheju (제주산 온주밀감의 특성과 관능평가)

  • Koh, Jeong-Sam;Koh, Jeong-Eun;Yang, Sang-Ho;Ahn, Sung-Ung
    • Applied Biological Chemistry
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    • v.37 no.3
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    • pp.161-167
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    • 1994
  • Soluble solids $(^{\circ}Brix)$, acid content and vitamin C content of Citrus miyakawa unshiu and C. okitsu unshiu juice harvested middle of November 1993 in Seuho-Dong and Topyung-Dong, Seogwipo-si, south area of Cheju were $10.7{\sim}11.0$, 1.04% and $41.19{\sim}44.94\;mg/100\;g$ juice, respectively. Carbohydrate was consisted of about 1/2 sucrose, 1/4 glucose, and 1/4 fructose. Fruit weight, peel thickness, soluble solids, pH, acid content and Brix/Acid ratio had a good correlation in linear function with increasing fruit size, respectively. However, hardness and edible part ratio were not showed correlation with increasing fruit size. Middle size of citrus fruit was favorable to panelists, and the citrus peel color of red yellow was more favorable than pale yellow color in sensory evaluation. Sensory evaluation score showed a linear correlation among citrus fruits of C. okitsu unshiu produced in Namwon-ri, Seuho-Dong, Seogwipo-si, south area of Cheju, and that of Chochun-ri, Ara-Dong, Cheju-si, north area of Cheju, and the score was higher according to citrus fruit produced in optimum cultivation area. With increasing Brix/Acid ratio of citrus juice, high sensory evaluation score was gained in panelists. These data obtained are supposed to be applied to the quality evaluation of Citrus unshiu produced in Cheju.

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