• 한국정보디스플레이학회:학술대회논문집
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• 2004.08a
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• pp.975-977
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• 2004

According to thin and light form-factor and additional function of today's electronic devices, it is required to decrease the pattern pitch of FPC. The high density demand is more and more important trend especially, for small size LCD module. Based on this requirement, the manufacturing process is advancing from subtractive method to super subtractive method.

• Journal of the Korean Society of Manufacturing Process Engineers
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• v.20 no.2
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• pp.45-50
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• 2021

In the recent fourth industrial revolution, the demand for additive processes has emerged rapidly in many mechanical industries, including the aircraft and automobile industries. Additive processes, in contrast to subtractive processes, can be used to produce complex-shaped products, such as three-dimensional cooling systems and aircraft parts that are difficult to produce using conventional production technologies. However, the limitations of additive processes include nonuniform surface quality, which necessitates the use of post-processing techniques such as subtractive methods and grinding. This has led to the need for hybrid machines that combine additive and subtractive processes. A hybrid machine uses additional additive and subtractive modules, so product deformation, for instance, deflection, is likely to occur. Therefore, structural analysis and design optimization of hybrid machines are essential because these defects cause multiple problems, such as reduced workpiece precision during processing. In this study, structural analysis was conducted before the development of an additive/subtractive hybrid processing machine. In addition, structural optimization was performed to improve the stability of the hybrid machine.

• The Plant Pathology Journal
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• v.19 no.1
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• pp.34-42
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• 2003

The ascomycete Magnaporthe grisea is a pathogen of rice blast and is known to form specialized infection structures called appressoria for successful infection into host cells. To understand the molecular mechanism underlying infection process, appressorium-related genes were identified through global approaches including EST sequencing, differential hybridization, and sup-pression subtractive hybridization. EST database was generated on >2,000 cDNA clones randomly selected from appressorium stage cDNA library. Large number of ESTs showed homology to known proteins possibly involved in infection-related cellular development (attachment, germination, appressorium formation, and colonization) of rice blast fungus. The 1051 ESTs showing significant homology to known genes were assigned to 11 functional categories. Differential hybridization and suppression subtractive hybridization were applied to identify genes showing an appressorium stage specific expression pattern. A number of genes were selected as up-regulated during appressorium formation compared with the vegetative growing stage. Clones from various cDNA libraries constructed in different developmental stages were arrayed on slide glass for further expression profiling study. functional characterization of genes identified from these global approaches may lead to a better understand-ing of the infection process of this devastating plant disease, and the development of novel ways to protect host plant.

• Journal of Magnetics
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• v.12 no.1
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• pp.12-16
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• 2007

Magnetic excitation and reversal by a spin polarized current via spin transfer have been a central research topic in spintronics due to its application potential. Special techniques are required to fabricate nano-scale magnetic layers in which the effect can be observed and studied. This work discusses the possibility of using electron-beam resists, the nano-scale patterning media, as ion milling mask in a subtractive fabrication method. The possibility is demonstrated by two resists, one positive tone, the ZEP 520A, and one negative tone, the ma-N2403. The advantage and the key points for success of this process will be also addressed.

• Korean Institute of Interior Design Journal
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• v.25 no.2
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• pp.3-12
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• 2016

Aires Mateus Architects is one of the most well-known Portuguese architects after $\acute{A}lvaro$ Siza and Eduardo Souto de Moura. The studio shows clear design methods in which the relationship between solid mass and void space are creatively inter-connected. Subtractive transformation of form is the fundamental aspect of this relationship. Most of Aires Mateus' projects were designed based on the subtractive method. Although subtraction can be seen as architectural formal presentation, in Aires Mateus' cases, there are deeper logics, strategies, and meanings. Not only to create a form, subtractive method has been flexibly used to organize and compose different programs and spatial atmosphere. To examine this hidden dimension of Aires Mateus' unique design process, 5 case projects were selected and analyzed in detail based on program, light & visual-perception, and site's placeness. From the case study results, it is found that there are much deeper role of subtraction: relationship between solid-void are constantly changing to generate serviced and servant spaces, there are central spaces that are contrast against the complexity of exterior forms, aiming to build an archetypal form that transcends ever-changing time, ambivalent balance between universality and peculiarity in the genealogy of contemporary architecture. The study concluded that the unique characteristics of subtraction method as design organization have various possibilities that can apply on other architectural and interior projects which would have similar ideas and circumstances.

• Asian-Australasian Journal of Animal Sciences
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• v.26 no.7
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• pp.921-929
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• 2013

Huoyan goose is a Chinese local breed famous for its higher laying performance, but the problems of variety degeneration have emerged recently, especially a decrease in the number of eggs laid. In order to better understand the molecular mechanism that underlies egg laying in Huoyan geese, gene profiles in the pituitary gland of Huoyan geese taken during the laying period and ceased period were investigated using the suppression subtractive hybridization (SSH) method. Total RNA was extracted from pituitary glands of ceased period and laying period geese. The cDNA in the pituitary glands of ceased geese was subtracted from the cDNA in the pituitary glands of laying geese (forward subtraction); the reverse subtraction was also performed. After sequencing and annotation, a total of 30 and 24 up and down-regulated genes were obtained from the forward and reverse SSH libraries, respectively. These genes mostly related to biosynthetic process, cellular nitrogen compound metabolic process, transport, cell differentiation, cellular protein modification process, signal transduction, small molecule metabolic process. Furthermore, eleven genes were selected for further analyses by quantitative real-time PCR (qRT-PCR). The qRT-PCR results for the most part were consistent with the SSH results. Among these genes, Synaptotagmin-1 (SYT1) and Stathmin-2 (STMN2) were substantially over-expressed in laying period compared to ceased period. These results could serve as an important reference for elucidating the molecular mechanism of higher laying performance in Huoyan geese.

• Journal of the Korean Society for Precision Engineering
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• v.17 no.8
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• pp.46-52
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• 2000

3D Welding and Milling is a solid freeform fabrication process which is based on the combination of welding as additive and conventional milling as subtractive technique. This hybrid approach enables direct building of metallic parts with high accuracy and surface finish. Although it needs further improvements it shows an application potential in rapid tooling of injection mold inserts as the investigation results show. To optimize the process for higher surface quality and accuracy effectively Taguchi method is applied to the experimental investigation. in this way relationships between process parameters and final product qualities such as tensile strength and surface hardness are found with minimal efforts.

• Proceedings of the Korea Society of Environmental Toocicology Conference
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• 2003.05a
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• pp.107-107
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• 2003

The heavy metal cadmium is a xenobiotic toxicant of environmental and occupational concern and it has been classified as a human carcinogen. Inhalation of cadmium has been implicated in the development of emphysema and pulmonary fibrosis, but, the detailed mechanism by which cadmium induces adverse biological effects is not yet known. Therefore, we undertook the investigation of genes that are induced after cadmium exposure to illustrate the mechanism of cadmium toxicity For this purpose, we employed the polymerase chain reaction-based suppression subtractive hybridization technique. We identified 29 different cadmium-inducible genes in human peripheral mononuclear cells, such as macrophage migration inhibitory factor, lysophosphatidic acid acyltransferase-${\alpha}$, enolase-1${\alpha}$, VEGF, Bax, neuron-derived orphan receptor-1, and Nur77, which are known to be associated with inflammation, cell survival, and apoptosis. Induction of these genes by cadmium treatment was further confirmed by semi-quantitative reverse-transcription polymerase chain reaction. Further, we found that these genes were also induced after cadmium exposure in normal human lung fibroblast cell line, WI-38, suggesting potential use of this induction profile to monitor cadmium toxicity in the lung. Next, Nur77, one of cadmium-inducible genes, was further studied since the products of Nur77 are known to be involved in the apoptotic process of lung cells. Following cadmium treatment, Nur77 gene expression was increased at protein-level in A549 cells. Consistently, the reporter containing Nur77 binding sequence was activated by 2.5-fold after exposure to cadmium in reporter gene analysis by transient transfection experiments. When the plasmid encoding dominant negative Nur77 that represses the transcriptional function of wild-type Nur77 was transfected into A549 cells, the expression of Bax was significantly reduced, suggesting that induction of Nur77 was an important process in cadmium-induced apoptosis in the cells. Cadmium induced the expression of Nur77 in vivo, confirming the relevance of the data obtained in viro. Together our results suggest that Nur77 gene expression in exposure to cadmium leads apoptosis of lung cells which may cause pathological changes in lung.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
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• v.27 no.10
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• pp.552-557
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• 2015

The nanostereolithography process using a femtosecond laser has been shown to have strong merits for the direct fabrication of 2D/3D micro structures. In addition, a femtosecond laser provides efficient tools for precise micromachining owing to the advantages of a small and feeble heat effect zone. In this paper, we report an effective fabrication process of 3D micro optical and fluidic devices using nanostereolithography process composed of a dual stage system. Process conditions for additive and subtractive fabrication are examined. The Piezo stage scanning system is used for 3D micro-fabrication in unit area of sub-mm scale, and the motor stage is employed in fabrication on the scale of several mm. The misalignment between the pizeo- and motor- stages is revised through rotational transformation of CAD data in the unit domain. Here, the effectiveness of the proposed process is demonstrated through examples using 3D optical and microfluidic structures.

• Textile Coloration and Finishing
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• v.29 no.4
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• pp.276-283
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• 2017

The aim of this study is to develop an effective dyeing process for black color on the silk fabrics, using natural Indigo, Madder, Amur cork tree, Alder, Logwood, and Gallnut. All natural dye materials were water-extracted, concentrated, and freeze-dried. Seven different processes were carried out and the color differences(${\Delta}E$) of black color with each process from the chemical black dyed fabric were compared. The light, washing, and rubbing fastness were evaluated. The black dyeing of the silk fabrics was well carried and excellent black color was obtained in every methods. The profound black color could be obtained by the subtractive mixture of the three primary colors of red, yellow, and blue in the order of indigo(blue) - amur cork tree(yellow) - madder or lac(red). Black color was efficiently obtained by iron mordant when logwood or alder fruit was used. The color difference(${\Delta}E$) from the black color with chemical dyestuff was the lowest in the indigo - amur cork tree - lac - iron method. Light fastness and washing fastness were excellent in all dyeing processes, showing grade 5. The fastness to rubbing was excellent as shown grade 5 in the dry samples of logwood - iron process and alder fruit - iron process.