• Journal of Microbiology and Biotechnology
• /
• v.19 no.9
• /
• pp.898-903
• /
• 2009

Pullorum disease affecting poultry is caused by Salmonella enterica serovar Pullorum and results in severe economic loss every year, especially in countries with a developing poultry industry. The pathogenesis of S. Pullorum is not yet well defined, as the specific virulence factors still need to be identified. Thus, to isolate specific DNA fragments belonging to S. Pullorum, this study used suppression subtractive hybridization. As such, the genome of the S. Pullorum C79-13 strain was subtracted from the genome of Salmonella enterica serovar Gallinarum 9 and Salmonella enterica serovar Enteritidis CMCC(B) 50041, respectively, resulting in the identification of 20 subtracted fragments. A sequence homology analysis then revealed three types of fragment: phage sequences, plasmid sequences, and sequences with an unknown function. As a result, several important virulence-related genes encoding the IpaJ protein, colicin Y, tailspike protein, excisionase, and Rhs protein were identified that may play a role in the pathogenesis of S. Pullorum.

• Journal of the Optical Society of Korea
• /
• v.19 no.1
• /
• pp.38-44
• /
• 2015

Three-dimensional (3D) crosstalk is one of the main causes of visual fatigue and needs to be suppressed. The 3D crosstalk can be categorized into two different kinds according to its appearance-additive 3D crosstalk and subtractive 3D crosstalk. In this paper, we analyze the influence of different kinds of 3D crosstalk to the perceived level of visual fatigue in order to suppress the perceived 3D crosstalk effectively.

• The Transactions of the Korean Institute of Electrical Engineers P
• /
• v.57 no.2
• /
• pp.198-201
• /
• 2008

We investigated the operating characteristics of the flux-lock type superconducting fault current limiter(SFCL) with the parallel connection between the primary and secondary windings which are connected with two superconducting units in series. The parallel connection for current level increase of the flux-lock type SFCL is necessary to apply the SFCL into the power system. The resistance generated in superconducting units was dependent upon the winding direction of the primary and the secondary coils, which can reduce the power burden. The resistance of the superconducting elements in the subtractive polarity winding is higher than that of the additive polarity winding. The fault current limiting effect of the subtractive polarity winding is better than that of the additive polarity winding. From this results, we confirmed that the power capacity of the flux-lock type SFCL could be increased by the parallel connection of the superconducting units.

• Kyungpook Mathematical Journal
• /
• v.45 no.4
• /
• pp.561-570
• /
• 2005

Weak Crossed Product Algebras correspond to certain graphs called lower subtractive graphs. The properties of such algebras can be obtained by studying this kind of graphs ([4], [5]). In [1], the author showed that a weak crossed product is Frobenius and its restricted subalgebra is symmetric if and only if its associated graph has a unique maximal vertex. A special construction of these graphs came naturally and was known as standard lower subtractive graph. It was a deep question that when such a special graph possesses unique maximal vertex? This work is to answer the question partially and to give a particular characterization for such graphs at which the corresponding algebras are isomorphic. A graph that follows the mentioned characterization is called flexible. Flexibility is to some extend a generalization of the so-called Coxeter groups and its weak Bruhat ordering.

• Journal of Multimedia Information System
• /
• v.6 no.2
• /
• pp.87-90
• /
• 2019

Machine learning techniques have been applied in almost all the domains of human life to aid and enhance the problem solving capabilities of the system. The field of medical science has improved to a greater extent with the advent and application of these techniques. Efficient expert systems using various soft computing techniques like artificial neural network, Fuzzy Logic, Genetic algorithm, Hybrid system, etc. are being developed to equip medical practitioner with better and effective diagnosing capabilities. In this paper, a comparative study to evaluate the predictive performance of subtractive clustering based ANFIS hybrid system (SCANFIS) with Fuzzy C-Means (FCM) based ANFIS system (FCMANFIS) for Alzheimer disease (AD) has been taken. To evaluate the performance of these two systems, three parameters i.e. root mean square error (RMSE), prediction accuracy and precision are implemented. Experimental results demonstrated that the FCMANFIS model produce better results when compared to SCANFIS model in predictive analysis of Alzheimer disease (AD).

• Journal of Dental Rehabilitation and Applied Science
• /
• v.37 no.2
• /
• pp.73-80
• /
• 2021

Purpose: The purpose of this study is to compare the color stability of provisional restorative materials fabricated by subtractive and additive manufacturing. Materials and Methods: PMMA specimens by subtractive manufacturing and conventional method and bis-acryl specimens by additive manufacturing were fabricated each 20. After immersing specimens in the coffee solution and the wine solution, the color was measured as CIE Lab with a colorimeter weekly for 4 weeks. Color change was calculated and data were analyzed with one-way ANOVA and the Tukey multiple comparisons test (α = 0.05). Results: PMMA provisional prosthetic materials by subtractive manufacturing showed superior color stability compared to bis-acryl provisional prosthetic materials by additive manufacturing (P < 0.05), and showed similar color stability to the PMMA provisional prosthetic materials by conventional method (P > 0.05). Conclusion: It is recommended to fabricate provisional restorations by subtractive manufacturing in areas where esthetics is important, such as anterior teeth, and consideration of the color stability will be required when making provisional prosthetic using additive manufacturing.

• Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
• /
• v.20 no.9
• /
• pp.70-77
• /
• 2006

We have fabricated an integrated three-phase flux-lock type SFCL, which consists of an YBCO($YB_a2Cu_3O_7$) thin film and a flux-lock reactor wound around an iron core of each phase. In order to apply the SFCL in a real power system, fault analyses for the three-phase system are essential. The short-circuit currents were effectively limited by adjusting the numbers of winding of each secondary coil and their winding directions. The flux flow generated in the iron core cancelled out under the normal operation due to the parallel connection between primary and secondary windings. However, the flux-lock type SFCL with same iron core was operated just after the fault due to the flux generating in the iron core. To analyze the current limiting characteristics, the additive polarity winding was compared with the subtractive one in the flux lock reactor. Whenever a single line-to-ground fault occurred in any phase, the peak value of the line current of the fault phase in the additive polarity winding increased up to about 12.87 times during the first-half cycle. On the other hand, the peak value in the subtractive polarity winding increased up to about 34.07 times under the same conditions. This is because the current flow between the primary and the secondary windings changed to additive or subtractive status according to the winding direction. We confirmed that the current limiting behavior in the additive polarity winding was more effective for a single-line-to-ground fault

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
• /
• v.18 no.3
• /
• pp.269-275
• /
• 2005

A flux lock-type SFCL consists of two coils which are wound in parallel each other through an iron core, and a HTSC thin film connects in series with coil 2. The operation of the flux-lock type SFCL can be divided into the subtractive polarity winding and the additive polarity winding operations according to the winding directions between coil 1, coil 2. When a fault occurs, the fault current in the HTS thin film exceeds the critical current so that resistance is generated in the HTS film, and thereby the fault current is limited by an instant rise in the impedance of the flux-lock type SFCL. We investigated he variances of initial fault current limiting instant according to the ratio of inductance of coil 1 and coil 2 in the flux-lock type SFCL. It was confirmed from experiments that the initial fault current limiting instant in the subtractive polarity and additive polarity windings were faster as the ratio of coil 2' inductance for coil 1's inductance increased. The 1st peak of fault current in case of the subtractive polarity winding was higher as the ratio of coil 2's inductance for coil 1's inductance increased. On the other hand, in case of the additive polarity winding, the 1st peak of fault current was lower.

• The Journal of Advanced Prosthodontics
• /
• v.9 no.6
• /
• pp.463-469
• /
• 2017

PURPOSE. To evaluate the fit of a three-unit metal framework of fixed dental prostheses made by subtractive and additive manufacturing. MATERIALS AND METHODS. One master model of metal was fabricated. Twenty silicone impressions were made on the master die, working die of 10 poured with Type 4 stone, and working die of 10 made of scannable stone. Ten three-unit wax frameworks were fabricated by wax-up from Type IV working die. Stereolithography files of 10 three-unit frameworks were obtained using a model scanner and three-dimensional design software on a scannable working die. The three-unit wax framework was fabricated using subtractive manufacturing (SM) by applying the prepared stereolithography file, and the resin framework was fabricated by additive manufacturing (AM); both used metal alloy castings for metal frameworks. Marginal and internal gap were measured using silicone replica technique and digital microscope. Measurement data were analyzed by Kruskal-Wallis H test and Mann-Whitney U-test (${\alpha}=.05$). RESULTS. The lowest and highest gaps between premolar and molar margins were in the SM group and the AM group, respectively. There was a statistically significant difference in the marginal gap among the 3 groups (P<.001). In the marginal area where pontic was present, the largest gap was $149.39{\pm}42.30{\mu}m$ in the AM group, and the lowest gap was $24.40{\pm}11.92{\mu}m$ in the SM group. CONCLUSION. Three-unit metal frameworks made by subtractive manufacturing are clinically applicable. However, additive manufacturing requires more research to be applied clinically.

• The Korean Journal of Malacology
• /
• v.29 no.3
• /
• pp.181-187
• /
• 2013

In order to investigate environmental stress inducible genes in abalone, we analyzed differentially expressed transcripts from a Pacific abalone, Haliotis discus hannai, after exposure to heat-, cold- or hyposalinity-shock by suppression subtractive hybridization (SSH) method. 1,074 unique sequences from SSH libraries were composed to 115 clusters and 986 singletons, the overall redundancy of the library was 16.3%. From the BLAST search, of the 1,316 ESTs, 998 ESTs (75.8%) were identified as known genes, but 318 clones (24.2%) did not match to any previously described genes. From the comparison results of ESTs pattern of three SSH cDNA libraries, the most abundant EST was different in each SSH library: small heat shock protein p26 (sHSP26) in heat-shock, trypsinogen 2 in cold-shock, and actin in hyposalinity SSH cDNA library. Based on sequence similarities, several response-to-stress genes such as heat shock proteins (HSPs) were identified commonly from the abalone SSH libraries. HSP70 gene was induced by environmental stress regardless of temperature-shock or salinity-stress, while the increase of sHSP26 mRNA expression was not detected in cold-shock but in heat-shock condition. These results suggest that the suppression subtractive hybridization method is an efficient way to isolate differentially expressed gene from the invertebrate environmental stress-response transcriptome.