• Journal of Microbiology and Biotechnology
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• 제19권9호
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• pp.898-903
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• 2009

Pullorum disease affecting poultry is caused by Salmonella enterica serovar Pullorum and results in severe economic loss every year, especially in countries with a developing poultry industry. The pathogenesis of S. Pullorum is not yet well defined, as the specific virulence factors still need to be identified. Thus, to isolate specific DNA fragments belonging to S. Pullorum, this study used suppression subtractive hybridization. As such, the genome of the S. Pullorum C79-13 strain was subtracted from the genome of Salmonella enterica serovar Gallinarum 9 and Salmonella enterica serovar Enteritidis CMCC(B) 50041, respectively, resulting in the identification of 20 subtracted fragments. A sequence homology analysis then revealed three types of fragment: phage sequences, plasmid sequences, and sequences with an unknown function. As a result, several important virulence-related genes encoding the IpaJ protein, colicin Y, tailspike protein, excisionase, and Rhs protein were identified that may play a role in the pathogenesis of S. Pullorum.

• Journal of the Optical Society of Korea
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• 제19권1호
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• pp.38-44
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• 2015

Three-dimensional (3D) crosstalk is one of the main causes of visual fatigue and needs to be suppressed. The 3D crosstalk can be categorized into two different kinds according to its appearance-additive 3D crosstalk and subtractive 3D crosstalk. In this paper, we analyze the influence of different kinds of 3D crosstalk to the perceived level of visual fatigue in order to suppress the perceived 3D crosstalk effectively.

• 전기학회논문지P
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• 제57권2호
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• pp.198-201
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• 2008

We investigated the operating characteristics of the flux-lock type superconducting fault current limiter(SFCL) with the parallel connection between the primary and secondary windings which are connected with two superconducting units in series. The parallel connection for current level increase of the flux-lock type SFCL is necessary to apply the SFCL into the power system. The resistance generated in superconducting units was dependent upon the winding direction of the primary and the secondary coils, which can reduce the power burden. The resistance of the superconducting elements in the subtractive polarity winding is higher than that of the additive polarity winding. The fault current limiting effect of the subtractive polarity winding is better than that of the additive polarity winding. From this results, we confirmed that the power capacity of the flux-lock type SFCL could be increased by the parallel connection of the superconducting units.

• Kyungpook Mathematical Journal
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• 제45권4호
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• pp.561-570
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• 2005

Weak Crossed Product Algebras correspond to certain graphs called lower subtractive graphs. The properties of such algebras can be obtained by studying this kind of graphs ([4], [5]). In [1], the author showed that a weak crossed product is Frobenius and its restricted subalgebra is symmetric if and only if its associated graph has a unique maximal vertex. A special construction of these graphs came naturally and was known as standard lower subtractive graph. It was a deep question that when such a special graph possesses unique maximal vertex? This work is to answer the question partially and to give a particular characterization for such graphs at which the corresponding algebras are isomorphic. A graph that follows the mentioned characterization is called flexible. Flexibility is to some extend a generalization of the so-called Coxeter groups and its weak Bruhat ordering.

• Journal of Multimedia Information System
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• 제6권2호
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• pp.87-90
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• 2019

Machine learning techniques have been applied in almost all the domains of human life to aid and enhance the problem solving capabilities of the system. The field of medical science has improved to a greater extent with the advent and application of these techniques. Efficient expert systems using various soft computing techniques like artificial neural network, Fuzzy Logic, Genetic algorithm, Hybrid system, etc. are being developed to equip medical practitioner with better and effective diagnosing capabilities. In this paper, a comparative study to evaluate the predictive performance of subtractive clustering based ANFIS hybrid system (SCANFIS) with Fuzzy C-Means (FCM) based ANFIS system (FCMANFIS) for Alzheimer disease (AD) has been taken. To evaluate the performance of these two systems, three parameters i.e. root mean square error (RMSE), prediction accuracy and precision are implemented. Experimental results demonstrated that the FCMANFIS model produce better results when compared to SCANFIS model in predictive analysis of Alzheimer disease (AD).

• 구강회복응용과학지
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• 제37권2호
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• pp.73-80
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• 2021

목적: 본 연구의 목적은 CAD-CAM 방식 중 절삭가공과 적층가공으로 제작한 임시 보철 재료의 색 안정성을 비교 연구하는 것이다. 연구 재료 및 방법: 절삭가공을 통해 제작한 PMMA계열, DLP타입 프린터와 광경화성 수지를 이용해 적층가공으로 제작한 bis-acryl계열 그리고 전통적인 방법으로 제작한 PMMA계열의 시편을 각 20개씩 제작하였다. 시편을 커피용액과 와인용액에 침전시킨 후, 비색계를 이용하여 4주간 매주 색 측정을 시행하였다. 색 변화(ΔE^*)값의 비교분석을 위해 One-way ANOVA와 사후 검정으로 Tukey test를 실시하였다(α = 0.05). 결과: 절삭가공을 통해 제작된 PMMA계열의 시편은 적층가공을 통해 제작된 bis-acryl계열의 시편에 비해 뛰어난 색 안정성을 보였고(P < 0.05), 전통적인 방법을 통해 제작된 PMMA계열의 시편과 유사한 색 안정성을 보였다(P > 0.05). 결론: 전치부와 같이 심미가 중요한 부위에서는 절삭가공을 통한 임시 보철물의 제작을 추천하며, 적층가공을 이용한 임시 보철물 제작 시에는 색 안정성에 대한 고려가 필요하다.

• 조명전기설비학회논문지
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• 제20권9호
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• pp.70-77
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• 2006

We have fabricated an integrated three-phase flux-lock type SFCL, which consists of an YBCO($YB_a2Cu_3O_7$) thin film and a flux-lock reactor wound around an iron core of each phase. In order to apply the SFCL in a real power system, fault analyses for the three-phase system are essential. The short-circuit currents were effectively limited by adjusting the numbers of winding of each secondary coil and their winding directions. The flux flow generated in the iron core cancelled out under the normal operation due to the parallel connection between primary and secondary windings. However, the flux-lock type SFCL with same iron core was operated just after the fault due to the flux generating in the iron core. To analyze the current limiting characteristics, the additive polarity winding was compared with the subtractive one in the flux lock reactor. Whenever a single line-to-ground fault occurred in any phase, the peak value of the line current of the fault phase in the additive polarity winding increased up to about 12.87 times during the first-half cycle. On the other hand, the peak value in the subtractive polarity winding increased up to about 34.07 times under the same conditions. This is because the current flow between the primary and the secondary windings changed to additive or subtractive status according to the winding direction. We confirmed that the current limiting behavior in the additive polarity winding was more effective for a single-line-to-ground fault

• 한국전기전자재료학회논문지
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• 제18권3호
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• pp.269-275
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• 2005

A flux lock-type SFCL consists of two coils which are wound in parallel each other through an iron core, and a HTSC thin film connects in series with coil 2. The operation of the flux-lock type SFCL can be divided into the subtractive polarity winding and the additive polarity winding operations according to the winding directions between coil 1, coil 2. When a fault occurs, the fault current in the HTS thin film exceeds the critical current so that resistance is generated in the HTS film, and thereby the fault current is limited by an instant rise in the impedance of the flux-lock type SFCL. We investigated he variances of initial fault current limiting instant according to the ratio of inductance of coil 1 and coil 2 in the flux-lock type SFCL. It was confirmed from experiments that the initial fault current limiting instant in the subtractive polarity and additive polarity windings were faster as the ratio of coil 2' inductance for coil 1's inductance increased. The 1st peak of fault current in case of the subtractive polarity winding was higher as the ratio of coil 2's inductance for coil 1's inductance increased. On the other hand, in case of the additive polarity winding, the 1st peak of fault current was lower.

• The Journal of Advanced Prosthodontics
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• 제9권6호
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• pp.463-469
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• 2017

PURPOSE. To evaluate the fit of a three-unit metal framework of fixed dental prostheses made by subtractive and additive manufacturing. MATERIALS AND METHODS. One master model of metal was fabricated. Twenty silicone impressions were made on the master die, working die of 10 poured with Type 4 stone, and working die of 10 made of scannable stone. Ten three-unit wax frameworks were fabricated by wax-up from Type IV working die. Stereolithography files of 10 three-unit frameworks were obtained using a model scanner and three-dimensional design software on a scannable working die. The three-unit wax framework was fabricated using subtractive manufacturing (SM) by applying the prepared stereolithography file, and the resin framework was fabricated by additive manufacturing (AM); both used metal alloy castings for metal frameworks. Marginal and internal gap were measured using silicone replica technique and digital microscope. Measurement data were analyzed by Kruskal-Wallis H test and Mann-Whitney U-test (${\alpha}=.05$). RESULTS. The lowest and highest gaps between premolar and molar margins were in the SM group and the AM group, respectively. There was a statistically significant difference in the marginal gap among the 3 groups (P<.001). In the marginal area where pontic was present, the largest gap was $149.39{\pm}42.30{\mu}m$ in the AM group, and the lowest gap was $24.40{\pm}11.92{\mu}m$ in the SM group. CONCLUSION. Three-unit metal frameworks made by subtractive manufacturing are clinically applicable. However, additive manufacturing requires more research to be applied clinically.

• 한국패류학회지
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• 제29권3호
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• pp.181-187
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• 2013

In order to investigate environmental stress inducible genes in abalone, we analyzed differentially expressed transcripts from a Pacific abalone, Haliotis discus hannai, after exposure to heat-, cold- or hyposalinity-shock by suppression subtractive hybridization (SSH) method. 1,074 unique sequences from SSH libraries were composed to 115 clusters and 986 singletons, the overall redundancy of the library was 16.3%. From the BLAST search, of the 1,316 ESTs, 998 ESTs (75.8%) were identified as known genes, but 318 clones (24.2%) did not match to any previously described genes. From the comparison results of ESTs pattern of three SSH cDNA libraries, the most abundant EST was different in each SSH library: small heat shock protein p26 (sHSP26) in heat-shock, trypsinogen 2 in cold-shock, and actin in hyposalinity SSH cDNA library. Based on sequence similarities, several response-to-stress genes such as heat shock proteins (HSPs) were identified commonly from the abalone SSH libraries. HSP70 gene was induced by environmental stress regardless of temperature-shock or salinity-stress, while the increase of sHSP26 mRNA expression was not detected in cold-shock but in heat-shock condition. These results suggest that the suppression subtractive hybridization method is an efficient way to isolate differentially expressed gene from the invertebrate environmental stress-response transcriptome.