• BMB Reports
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• v.53 no.12
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• pp.611-621
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• 2020

Bacterial endoribonuclease toxins belong to a protein family that inhibits bacterial growth by degrading mRNA or rRNA sequences. The toxin genes are organized in pairs with its cognate antitoxins in the chromosome and thus the activities of the toxins are antagonized by antitoxin proteins or RNAs during active translation. In response to a variety of cellular stresses, the endoribonuclease toxins appear to be released from antitoxin molecules via proteolytic cleavage of antitoxin proteins or preferential degradation of antitoxin RNAs and cleave a diverse range of mRNA or rRNA sequences in a sequence-specific or codon-specific manner, resulting in various biological phenomena such as antibiotic tolerance and persister cell formation. Given that substrate specificity of each endoribonuclease toxin is determined by its structure and the composition of active site residues, we summarize the biology, structure, and substrate specificity of the updated bacterial endoribonuclease toxins.

• Transactions of Materials Processing
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• v.25 no.6
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• pp.353-358
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• 2016

Direct energy deposition (DED) is an additive manufacturing technique that involves the melting of metal powder with a high-powered laser beam and is used to build a variety of components. In recent year, it can be widely used in order to produce hard, wear resistant and/or corrosion resistant surface layers of metallic mechanical parts, such as dies and molds. For the purpose of the hardfacing to achieve high wear resistance and hardness, application of high speed steel (HSS) can be expected to improve the tool life. During the DED process using the high-carbon steel, however, defects (delamination or cracking) can be induced by rapid solidification of the molten powder. Thus, substrate preheating is generally adopted to reduce the deposition defect. While the substrate preheating ensures defect-free deposition, it is important to select the optimal preheating temperature since it also affects the microstructure evolution and mechanical properties. In this study, AISI M4 powder was deposited on the AISI 1045 substrate preheated at different temperatures (room temperature to $500^{\circ}C$). In addition, the micro-hardness distribution, cooling rates, and microstructures of the deposited layers were investigated in order to observe the influence of the substrate preheating on the mechanical and metallurgical properties.

• Journal of the Institute of Electronics Engineers of Korea TC
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• v.47 no.10
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• pp.67-73
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• 2010

In this paper, the effect of a finite substrate on the mutual coupling of a pair of microstrip patch antennas along the H -plane is investigated. The mutual coupling of a pair of microstrip patch antennas can be reduced using the interference effect due to the phase difference by a variety of routes of the surface wave. In the case of the substrate with $\varepsilon_r$=10 and thickness of 3.2 mm, the mutual coupling is reduced by 4.85 dB on the substrate size with the strong mutual coupling, while the mutual coupling is reduced by 34.28 dB on the substrate size with the weak mutual coupling when the distance between the antenna centers is varied from 0.5 $\lambda_0$ to 1.0 $\lambda_0$. In the case of optimization substrate size, the decreasing rate of the mutual coupling with the increase of the distance between the antenna centers is very large. Good agreements between the image method and full wave simulation results are obtained.

• The Korean Journal of Mycology
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• v.43 no.2
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• pp.104-111
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• 2015

In this study, a new strain of Lentinula edodes (Shiitake) Poongnyunko was bred from dikaryotic KFRI 490 and monokaryotic KFRI 536 by Di-mon crossing method. In this study, the productivity was conducted through bed-log and sawdust substrate cultivation. The Poongnyunko showed significant intensive production from summer to autumn. Optimum temperatures of fruit-body formation were $17{\sim}24^{\circ}C$. The total amount of fruit-body production during 4 years by bed-log cultivation was $22kg/m^3$, and total yield of fruit-body until the 3rd flush was 648 g/ 2 kg. In case of sawdust substrate cultivation, the mycelial incubation period was 110~130 days. The suitable temperature and relative humidity were $18{\pm}1^{\circ}C$ and $90{\pm}5%$ respectively.

• Proceedings of the KIEE Conference
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• 1996.07c
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• pp.1618-1620
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• 1996

A variety of processing techniques have been reported for preparing high quality functional thin films, and one of the most successful techniques has been known to be the rf FTMS(facing targets magnetron sputtering) method. The rf FTMS has preferable advantages to reduce the resputtering effect when depositing thin films and efficiently to oxidize the grown films by oxygen radicals. The resulting optimum conditions were found to be the rf power 50 W and the substrate position of 20 mm.

• Journal of Microbiology and Biotechnology
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• v.27 no.11
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• pp.1907-1915
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• 2017

Lipases are important enzymes with biotechnological applications in dairy, detergent, food, fine chemicals, and pharmaceutical industries. Specifically, hormone-sensitive lipase (HSL) is an intracellular lipase that can be stimulated by several hormones, such as catecholamine, glucagon, and adrenocorticotropic hormone. Bacterial hormone-sensitive lipases (bHSLs), which are homologous to the C-terminal domain of HSL, have ${\alpha}/{\beta}-hydrolase$ fold with a catalytic triad composed of His, Asp, and Ser. These bHSLs could be used for a wide variety of industrial applications because of their high activity, broad substrate specificity, and remarkable stability. In this review, the relationships among HSLs, the microbiological origins, the crystal structures, and the biotechnological properties of bHSLs are summarized.

• Analytical Science and Technology
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• v.18 no.6
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• pp.460-468
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• 2005

The biotin-avidin complex, as a model recognition system, has been constructed through N-hydroxysuccinimide(NHS) reaction on a variety of substrates such as a smooth Au film, electrochemically roughened Au electrode and chemically modified mica. Stepwise self-assembled monolayers (SAMs) of biotin-avidin system were characterized by surface-enhanced resonance Raman scattering (SERRS) spectroscopy, atomic force microscopy (AFM) and surface plasmon resonance (SPR). A strong SERRS signal of rhodamine tags labeled in avidin from the SAMs on a roughened gold electrode indicated the successful complex formation of stepwise biotin-avidin recognition system. AFM images showed the circular shaped avidin aggregates (hexamer) with ca. $60{\AA}$ thick on the substrate, corresponding to one layer of avidin. The surface coverage and concentration of avidin molecules were estimated to be 90% and $7.5{\times}10^{-12}mol/cm^2$, respectively. SPR technique allowed one to monitor the surface reaction of the specific recognition with high sensitivity and precision.

• Journal of the Korean Institute of Navigation
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• v.20 no.4
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• pp.129-137
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• 1996

In this paper, we simulated a radiation pattern directivity of microstrip patch antenna for 9[GHz] using the computer. It is analyzed and compared the directivity of field characteristics at two different aspects. One is that of 1-dimension and 2-dimension array, the other is that of a linear and planar array antenna. We tried the constants for the simulation of teflon substrate, dielectric constant ${\varepsilon}r=2.50$, ground plane conductor thickness t=0.03556, substrate height h=0.72188, dielectric loss degree $tan{\delta}$=0.00085, characteristics impedence of line $Zo=50({\Omega})$. The major advantage of microstrip patch antenna is that the arrangement of antenna is associated with the directivity and effectiveness. We have been studied the difference of the radiation pattern of antenna with wave variety through computer simulation.

• Journal of the Korean Society of Manufacturing Process Engineers
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• v.13 no.2
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• pp.41-48
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• 2014

Direct writing(DW) is a method of patterning materials to a substrate directly, without a mask. It can use a variety of materials and be applied to various fields. Among DW systems, the flow-based type, using a syringe pump and nozzle, is simpler than other types. Furthermore, the range of materials is exceptionally wide. In additive processes, a three dimensional structure is made of stacking layer. Each layer is made of several lines. In this regard, good surface roughness of fabricated layers is essential to three dimensional fabrication. The surface roughness of any fabricated layer tends to change with the dispensing pattern. When multiple layers fabricated by a nozzle dispensing system are stacked, control of the nozzle position from the substrate is important in order to avoid interference between the nozzle and the fabricated layer. In this study, a fluid direct writing system for three dimensional structure fabrication was developed. Experimentsto control the position of the nozzle from substrate were conducted in order to examine the characteristics of the material used in this system.

• Proceedings of the Korean Vacuum Society Conference
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• 2014.02a
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• pp.424.1-424.1
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• 2014

Vanadium dioxide (VO2) is a strongly correlated oxide exhibiting a first-order metal-insulator transition (MIT) that is accompanied by a structural phase transition from a low temperature monoclinic phase to a high-temperature rutile phase. VO2 has attracted significant attention because of a variety of possible applications based on its ultrafast MIT. Interestingly, the transition nature of VO2 is significantly affected by stress due to doping and/or interaction with a substrate and/or surface tension as well as defects. Accordingly, there have been considerable efforts to understand the influences of such factors on the phase transition and the fundamental mechanisms behind the MIT behavior. Here, we present the influences of oxygen deficiency, hydrogen doping, and substrate-induced stress on MIT phenomena in single-crystalline VO2 nanobeams. Specifically, the work function and the electrical resistance of the VO2 nanobeams change with the compositional variation due to the oxygen-deficiency-related defects. In addition, the VO2 nanobeams during exposure to hydrogen gas exhibit the reduction of transition temperature and the complex phase inhomogenieties arising from both substrate-induced stress and the formation of the hydrogen doping-induced metallic rutile phase.