• Journal of Applied Biological Chemistry
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• 제59권1호
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• pp.57-62
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• 2016

In this study, we optimized fermentation conditions for the solid state fermentation of rice bran with Monascus pilosus KCCM60084, and the antioxidant activities were investigated. Optimal fermentation conditions were determined by the production of Monacolin K, a functional secondary metabolites with cholesterol lowering activity. The highest Monacolin K production were 2.88 mg/g observed on day 10 with 45% moisture content in the substrate when inoculated with 5% inoculum (w/w). Reducing power, iron chelating activity and $ABTS^+$ radical scavenging activity were significantly enhanced after fermentation by 60, 80, and 38% respectively. Furthermore, the content of total flavonoid were found to be increased by 4.58 fold. Based on these results, Monascus-fermented rice bran showed strong possibility to be used as a natural antioxidant agent due to its enhanced antioxidant activity.

• 한국환경과학회지
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• 제33권1호
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• pp.17-25
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• 2024

This study aimed to determine the most suitable coir substrate mixing ratio for optimizing the growth and yield of the "lpduelkkae 1" cultivar. We comprehensively analyzed the physicochemical properties, growth, and yields of four different substrate combinations: perlite (coir with mixing ratios of 70:30 (PC30), 50:50 (PC50), and 30:70 (PC70)) and 100% coir (C100). The results revealed substantial differences in substrate properties. C100 exhibited the highest total porosity and the lowest solid phase, indicating excellent air permeability. The pH levels and electrical conductivity (EC) values ranged from 5.4-6.8 and 1.2-3.1 dS·m^-1, respectively. Leaf growth parameters, including length, width, and dry weight, showed positive correlations with high coir ratios, except for PC30. PC70 and C100 outperformed other substrates in stem growth, exhibiting superior stem diameter and fresh and dry weights. The quantity of marketable leaves was the highest in the C100 substrate. Furthermore, C100 comprised integrated levels of essential nutrients, such as Ca and Mg, owing to its high coir content. In conclusion, a coir ratio of approximately 70% (v/v) should be maintained in the substrate for creating an optimal cultivation environment. Furthermore, the selection of humidity-resistant varieties as well as precise nutrient and moisture management for different seasons and growth stages are crucial for a successful perilla leaf hydroponic cultivation.

• 한국버섯학회지
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• 제13권3호
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• pp.233-236
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• 2015

본 보고는 버섯 병재배용 배지혼합기를 사용하여 배지를 조제할 때 매일 배지의 수분함량을 일정하게 유지하고자 로드셀을 장치하여 활용하는 방법을 고안한 결과이다. 배지혼합기의 하부에 로드셀을 장치하고 병당 입병량과 입병수로 계산한 무게에 도달했을 때 물 주입이 자동적으로 끝나도록 하는 장치를 개발하였다. 또한 배지재료별 수분함량을 측정하고 프로그램(Cheong et al., 2015)을 활용하여 배지 조제시 목표로 하는 수분함량에 도달하는 배지의 총 무게를 산출하였다. 이 방법은 로드셀의 표시부에 목표 수분함량에 따른 배지의 총 무게를 입력한 후, 물공급을 시작하여 혼합배지가 입력해 둔 무게에 도달하면 물 공급이 중단된다. 또한 물 공급을 자동화하여 반복되는 배지 조제 작업에서 사용자의 수고를 경감시켜 편의성을 향상시킬 수 있다. 그리고 버섯 종류별 배지 조성별로 혼합 배지의 알맞은 수분 함량의 정확도를 향상시킬 수 있고, 균 배양 기간, 버섯 발생 및 생육 기간이 균일하게 유지되어 배지를 안정적으로 생산할 수 있다. 따라서, 연중 버섯 품질 및 수량 안정으로 버섯 농가의 경영 안정을 도모할 수 있다.

• 한국작물학회지
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• 제53권3호
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• pp.339-346
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• 2008

현미, 쌀보리, 옥수수, 고구마작물의 전분 함량과 일반성분 함량분석이 에탄올 생산에 미치는 영향을 조사하였다. 1. 단순 회귀분석 결과에 의해서 각 원료에서 전분 함량이 에탄올 생산량에 유의하게 영향을 주는 것으로 나타났는데, 전분 함량이 높을수록 에탄올 생산이 증가하였다. 2. 쌀보리의 경우 단백질 함량이 낮을수록 에탄올 생산이 많이 되는 것으로 나타났으며, 특이하게도 조섬유의 경우는 단백질 함량과 반대로 조섬유 함량이 높을수록 에탄올 생산이 높았다. 3. 현미의 경우, 회분 함량이 높을수록 에탄올 생산이 낮게 나타났다. 4. 고구마의 경우, 수분 함량이 다른 원료에 비하여 상대적으로 뚜렷이 높았으며, 수분 함량이 높을수록 에탄올 생산량은 낮아지는 것으로 분석되었다. 5. 건조 전 작물 중량을 기준으로 할 때 가장 높은 에탄올 생산수율은 현미에서 나타났고, 이중에서도 다산 현미가 439.3 liter/ton으로 가장 높았고, 그 다음 옥수수 그리고 보리의 순이었으며, 수분함량이 높은 고구마의 경우 전분함량이 상대적으로 낮아 에탄올 생산량이 낮게 분석되었다. 6. 건조 후 작물 중량 기준 시에는 건미 고구마가 530.6 l/ton으로 가장 높았고, 그 다음이 다산 현미 등의 현미, 정미 및 바이오미의 고구마, 옥수수, 보리의 순이었다. 7. 단위면적당 에탄올 생산량 성적을 살펴보면 현미가 평균 2560.0 l/ha, 쌀보리가 평균 1523.6 l/ha, 옥수수가 평균 2992.3 l/ha, 고구마가 평균 4169.1 l/ha로 나타났으며, 이중에서 건미 고구마가 5115.7 l/ha로 가장 높은 단위면적당 에탄올 생산량을 보였다.

• 한국균학회소식:학술대회논문집
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• 한국균학회 2018년도 춘계학술대회 및 임시총회
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• pp.29-29
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• 2018

Sparassis latifolia is called "Cauliflower Mushroom" and is known as an edible mushroom that has high content of ${\beta}$-glucan. Recently, artificial cultivation of S. latifolia has been done by bottle, plastic bag and wood cultivation in Korea. However it is not widely used because there are low incubation ratio and yield. For the high efficiency of production, we aim to find the superior strains and media for better mycelial and fruit body growth. First, we analyzed the genetic relationship among 31 strains and divided five groups with three kinds of URP primers. And then ten strains were selected from five groups based on the experiment of mycelial growth. The suitability of media for mycelial growth was different according to media type. The suitable solid and liquid media for mycelial growth of S. latifolia isolates were PDA and M2, respectively. In addition, with regard to C/N ratio, the mycelial growth increased even until C/N 160. Second, we investigated the production of fruitbody of the strains by plastic bag cultivation. The substrate was mixed with larch sawdust, corn flour, and wheat flour (8:1:1, v/v). Moisture content of substrate was controlled by about 60% with 10% molasses solution. Out of 31 strains, 19 strains formed primordia. The eight strains produced more than 140g/1kg in fresh weight. Third, molasses culture media was selected for the mycelial growth. And molasses suitable sugar content and input aeration were around 8Brix% and 0.3~0.6vvm, respectively. The longer the incubation period is, the more dried weight of mycelia increased, but medium volume decreased. Therefore, the best incubation period was 9 to 11 days depending on strains. In the future, research project entitled development of culture system and new variety for stable production of S. latifolia will be considered as a new item.

• 미생물학회지
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• 제19권1호
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• pp.14-22
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• 1981

Protein content of cellulosic wastes, such as spent grain, hop bark, spent rye, rice straw, rice hull, saw dust and used newspaper, was increased by a mixed culture of C. utilis wastes having 66-75% moisture. Among the fungal strains tested. A.phoenicis KU175 was the most powerful to increase the protein content of A. phoenicis during the mixed culture with C. utilis in the CMC medium reached at the peak for one day culture after inoculation of the both strains at the same time, while it reached at peark from the beginning of the mixed culture, when A. phoenicis was inocultated for 12-24hours prior to the inoculation of C.utilis. To increase the protein content of the cellulosic wastes by the mixed culture of C.utilis and A.phoenicis, the inoculation of both strains at the same time was more effective than the preinoculation of A. phoenicis for 6-24 hours. Content of crude cellulose in the used newspaper, saw dust and spent grain was high relatively, and the lignin content of spent grain, spent rye, and rice strew was reduced more than half by the treatment of 2% NaOH. However, effect of alkali treatment of increase the protein content of the cellulosic wastes was not prominent in the case of mixed culture. Protein content of the cellulosic wastes was increased prominently by the mixed culture of C.utilis and A.phoenicis in semi-solid substrate, compared with the single culture of C. utilis, although the latter increased the protein content of cellulosic wastes considerably. The effect of mixed culture of C. utilis and A. phoenicis increased 4-fold the protein content of spent grain, and more than doubled crude protein in hop bark and rice straw.

• 한국환경과학회지
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• 제21권3호
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• pp.289-296
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• 2012

The purpose of this study was to identify the irrigation intervals and the amount of suitable growing substrate needed to achieve the desired shallow-extensive green roof system during a dry summer in Korea. In terms of treatment, three types (SL, $P_6P_2L_2$, $P_4P_4L_2$) with varying soil mixture ratios and two types (15 cm, 25 cm) with varying soil depths were created. The results have been analyzed after measuring growth and soil water contents. The difference of growth by treatment was significant in terms of green coverage, height, leaf width and photosynthesis. In measurement of chlorophyll content, no difference was detected when measured against soil depth. According to the growth measurement of Zoysia japonica with respect to differing soil mixture ratios in the 15 cm-deep treatment, a statistical difference was detected at the 0.05 significance level in photosynthesis. In case of green coverage, height, chlorophyll content and leaf width, no statistical significance was observed. In case of the 25 cm-deep treatment, a statistical significance was observed in height and photosynthesis. In terms of green coverage, chlorophyll content and leaf width, no statistical significance was detected. In comparisons of soil moisture tension and soil water contents, the irrigation interval and amount were 8 days and 14.9 L in the SL (15 cm) treatment, respectively. The irrigation interval showed for 10 days a 1.3-fold increase, and the irrigation amount was 27.4 L 1.8-fold more than SL (25 cm), respectively. For $P_6P_2L_2$ (15 cm) treatment, the irrigation interval and amount were 12 days and 20.7 L, respectively. However, an irrigation interval under $P_6P_2L_2$ (25 cm) was for 15 days 1.3 times longer than $P_6P_2L_2$ (15 cm), and an irrigation amount of 40 L was 1.9 times more than that under $P_6P_2L_2$ (15 cm). In $P_4P_4L_2$ (15 cm) treatment, it was indicated that the irrigation interval was 15 days, and the irrigation amount was 19.2 L. It was not needed to irrigate for 16 days under $P_4P_4L_2$ (25 cm) treatment during the dry summer and the longest no-rain periods. The irrigation interval and amount under $P_4P_4L_2$ were 1.8-fold and 1.3-fold, respectively, more than SL treatment as affected by soil mixture ratio. Comparatively $P_4P_4L_2$ had more 1.3-fold and 0.9-fold in irrigation interval and amount more than $P_6P_2L_2$. Therefore, it can be noted that different soil depth and soil mixture ratios had a significant effect on the irrigation interval and amount.

• 한국균학회지
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• 제26권2호통권85호
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• pp.265-274
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• 1998

목질진흙버섯(Phellinus sp.) 자실체의 인공재배를 위한 기초자료마련의 일환으로 자실체로부터 순수분리한 상황속의 한 균주를 이용하여 참나무 (Quercus aliena), 뽕나무(Morus alba) 및 오리나무(Alnus japonica)의 3종 톱밥기질에 따른 균사생육의 최적조건을 반응표면분석방법으로 조사하였다. 균사생육에 영향을 미치는 3종의 독립변수(쌀겨첨가량, 배지의 초기 pH 및 수분함량)와 그 최적값을 검토한 결과, 쌀겨첨가량은 3종의 톱밥배지 모두에서 약 30%(w/w) 첨가시에 가장 좋았으며, 수분함량은 $65{\sim}70%(w/v)$ 범위 에서 좋았다. 배지의 초기 pH는 참나무와 뽕나무에서는 $pH\;5{\sim}6$, 오리나무 톱밥배지의 경우는 pH 6에서 가장 좋았다. 균사의 생육온도는 모든 톱밥배지에서 $25{\sim}30^{\circ}C$의 온도범위에서 좋았으나, 톱밥수종에 따른 차이를 보였다. 균사생육에 영향을 미치는 쌀겨첨가량$(X_1)$, 배지의 초기 $pH(X_2)$ 및 수분함량 $(X_3)$을 각각 종속변수로 하고, 균사의 생육길이를 독립변수(Y)로 하여 반응 표면분석한 결과, 참나무 톱밥의 경우, 쌀겨첨가량 31.01%(w/w), 배지의 PH 5.31, 그리고 수분함량 69.03%(w/v)에서 얻어졌으며, 이때 기대되는 균사의 생육길이는 8.32cm이었다. 특히, 쌀겨 첨가량$(X_1)$과 수분함량$(X_3)$)의 회귀계수가 높아, 이 변수들이 균사생육에 큰 영향을 미침을 알 수 있었다. 뽕나무 톱밥배지의 경우, 쌀겨첨가량, 배지의 초기 pH 및 수분함량의 정상점은 각각 28.77%(w/w), 5.28및 69.8%(w/v)로, 예측되는 균사의 생육길이는 7.60 cm이었다. 오리나무 톱밥 배지의 정상점은 쌀겨첨가량 28.74%(w/w), 배지의 pH 6.04및 수분함량 66.96%(w/v)으로, 이때, 예상되는 균사의 생육길이는 5.38cm이었다. 이상의 결과로부터 목질진흙버섯의 균사 생육과 독립변수들 사이에는 상관관계가 높았고, 쌀겨첨가량과 배지의 초기 pH가 수분함량보다 균사생육에 더 큰 영향을 미쳤으며, 목질진흙버섯의 균사 생육을 위한 최적의 톱밥 수종은 참나무(Quercus aliena)>뽕나무(Morus alba)>오리나무(Alnus japonica)의 순이었다.

• Journal of Microbiology and Biotechnology
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• 제21권9호
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• pp.947-953
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• 2011

A fungal strain, Aspergillus terreus strain GA2, isolated from an agricultural field cultivating sweet sorghum, produced feruloyl esterase using maize bran. In order to obtain maximum yields of feruloyl esterase, the solid state fermentation (SSF) conditions for enzyme production were standardized. Effective feruloyl esterase production was observed with maize bran as substrate followed by wheat bran, coconut husk, and rice husk among the tested agro-waste crop residues. Optimum particle size of 0.71-0.3 mm and moisture content of 80% favored enzyme production. Moreover, optimum feruloyl esterase production was observed at pH 6.0 and a temperature of $30^{\circ}C$. Supplementation of potato starch (0.6%) as the carbon source and casein (1%) as the nitrogen source favored enzyme production. Furthermore, the culture produced the enzyme after 7 days of incubation when the C:N ratio was 5. Optimization of the SSF conditions revealed that maximum enzyme activity (1,162 U/gds) was observed after 7 days in a production medium of 80% moisture content and pH 6.0 containing 16 g maize bran [25% (w/v)] of particle size of 0.71-0.3 mm, 0.6% potato starch, 3.0% casein, and 64 ml of formulated basal salt solution. Overall, the enzyme production was enhanced by 3.2-fold as compared with un-optimized conditions.

• Journal of Welding and Joining
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• 제35권3호
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• pp.7-14
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• 2017

The effect of high temperature-moisture on corrosion and mechanical properties for Sn-0.7Cu, Sn-3.0Ag-0.5Cu (SAC305) solders on flexible substrate was studied using Highly Accelerated Temperature/Humidity Stress Test (HAST) followed by three-point bending test. Both Sn-0.7Cu and SAC305 solders produced the internal $SnO_2$ oxides. Corrosion occurred between the solder and water film near flexible circuit board/copper component. For the SAC305 solder with Ag content, furthermore, octahedral corrosion products were formed near Ag3Sn. For the SAC305 and Sn-0.7Cu solders, the amount of internal oxide increased with the HAST time and the amount of internal oxides was mostly constant regardless of Ag content. The size of the internal oxide was larger for the Sn-0.7Cu solder. Despite of different size of the internal oxide, the fracture time during three-point bending test was not significantly changed. It was because the bending crack was always initiated from the three-point corner of the chip. However, the crack propagation depended on the oxides between the flexible circuit board and the Cu chip. The fracture time of the three-point bending test was dependent more on the crack initiation than on the crack propagation.