• 식물병연구
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• 제24권3호
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• pp.213-220
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• 2018

섬유소 분해 점액세균인 Sorangium cellulosum 균주들 중 Botrytis cinerea에 길항력을 갖는다고 보고한 4균주(Antagonistic to Botrytis, AB 계열)와 Colletotrichum acutatum에 길항력을 갖는다고 보고한 2균주(Antagonistic to Colletotrichum, AC 계열)를 5가지(A-E)의 S. cellulosum 아종(subspecies)으로 분류하였다. 분류 기준 유전자인 xynB1, bglA2, groEL1 세 유전자의 유전자 유무 및 염기서열 분석을 통해 AB 계열은 아종 C, AC 계열은 아종 D로 분류하였다. 또한, 배양추출물을 고효율 액체크로마토그래피(HPLC)로 분석한 아종 분류 결과 AB 계열 4균주는 머무름시간 20-22.5분에 유사한 특징 피크가 나타난 반면, AC 계열인 2균주는 특징 피크가 없으므로 AB 계열은 아종 C, AC 계열은 아종 D임을 재확인하였다. AB 계열 4균주들의 배양여액을 사용하여 방울토마토에 전처리 후 B. cinerea를 접종한 생물검정으로 방제가와 크로마토그램 특징 피크의 상대 면적값과의 상관분석 결과, 피크 면적이 큰 균주일수록 방제가도 높은 양의 상관관계($R^2=0.9652$)를 확인하였다. 아종 분류 결과 AB 계열은 아종 C의 대표 생리활성 물질의 파생물인 epothilone D로 추정되어, 이를 표준시료로 HPLC 분석한 결과 epothilone D의 머무름시간은 9.9분이었던 반면, KYC 3270 특성 피크의 머무름시간은 11.581분으로 달랐다. 따라서 우리는 AB 계열이 분비하는 생리활성 물질은 epothilone의 파생물인 7-ketone epothilone D로 추정하였다.

• Animal Systematics, Evolution and Diversity
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• 제3권1호
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• pp.57-62
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• 1987

잔라북도 부안군의 백천에 서식하는 기름종개속 어류의 표본을 조사한바, 종검색의 기준이 되는 수컷 가슴지느러미 기부에 출현하는 골질반은 참종개 Cogbitis koreensis 와 비슷하지만, 조골수역의 기름종개 속 모든 표본은 체측반문의 모양이 특이하고, 척추골수가 37-40개로 적으며 체장범위가 85m 이하인 소형인 점이 참종개와는 잘 구별되어 신아종 C.koreensis pumilus n. subsp.(부안종개, 신칭)로 명명 기재한다.

• Animal Systematics, Evolution and Diversity
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• 제12권2호
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• pp.107-119
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• 1996

한국산 왕눈이반날개족에 대한 재검토 결과, 3속 10종 1아종으로 밝혀졌다. 이들 중, Veleeius circumipectus는 신종, Quedius japonicus sachonensis는 신아종이었으며, Heterothops rotundiceps, Q. japonicus(s.str.), Q. simulans, 및 V. setosus는 한국산 미기록 종이었다.

• Asian-Australasian Journal of Animal Sciences
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• 제11권5호
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• pp.475-479
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• 1998

Data on body weights were analyzed in the four genetic groups from all possible crosses of two subspecies of mice to estimate average direct genetic effects (ADGE), average maternal genetic effects (AMGE) and heterotic effect (HE). The genetic groups used were $CF_{{\sharp}1}$ laboratory mouse (Mus musculus domesticus), Yonakuni wild mouse (Yk, Mus musculus molossinus yonakuni) and two reciprocal $F_1$ crosses of them, CY and YC. First symbol in the reciprocal $F_1$ represent subspecies of dam. Body weight at 1 (Wk1), 3 (Wk3), 6 (Wk6) and 10 weeks of age (Wk10) were analyzed from 258 mice of the four genetic groups. The model used to evaluate body weights included main effects of genetic group and sex, and interaction effect between genetic group and sex. The ADGE and the AMGE were estimated as deviations of Yk from $CF_{{\sharp}1}$. The HE was estimated from the differences between the reciprocal $F_1$ and the midparent mean. Results of this study showed that all effects, except sex and interaction between genetic group and sex at Wk1 and Wk3, were highly significant source variation (p < 0.01). The ADGE were positive and highly significant (p < 0.01) at all ages studied for both sexes, while the AMGE were highly significant at Wk3, Wk6 and Wk10. The ADGE were larger in contributing effect on body weight differences than the AMGE. The positive value of the HE were observed at all ages for males, while for females the positive effects occured from birth through weaning.

• 한국동물위생학회지
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• 제41권3호
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• pp.203-210
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• 2018

Pasteurella multocida is an opportunistic organism that plays a significant role in porcine respiratory disease complex (PRDC). In the current study, we provide nationwide information of P. multocida isolates from pneumonic lungs of slaughter pigs by determining their prevalence, subspecies, biovars, capsular types, virulence-associated genes, and minimum inhibitory concentrations. P. multocida was the second most frequently confirmed (19.2%) bacterial pathogen and most of the isolates (88.9%) showed simultaneous infection with other respiratory pathogens, especially Mycoplasma hyopneumoniae (63.3%, P<0.001) and porcine circovirus type 2 (53.3%, P=0.0205). Of 42 isolates investigated, 41 (97.6%) were identified as P. multocida subspecies multocida, and only one isolate was identified as subspecies septica (biovar 5). All the isolates were capsular type A and the most prevalent biovar was biovar 3 (40.5%), followed by biovar 2 (31.0%). Comparing virulence-associated genes and biovars, all biovar 2 isolates exhibited $hgbB^-pfhA^+$ (P<0.001); all biovar 3 (P=0.0002) and biovar 13 (P=0.0063) isolates presented $hgbB^+pfhA^-$. Additionally, all biovar 2 (P=0.0037) isolates and most of biovar 3 (P=0.0265) isolates harbored tadD. P. multocida showed the highest resistance levels to oxytetracycline (73.8%), followed by florfenicol (11.9%). Continuous monitoring is required for surveillance of the antimicrobial resistance and new emerging strains of P. multocida in slaughter lines.

• Animal Systematics, Evolution and Diversity
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• 제8권1호
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• pp.35-56
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• 1992

한국 담수산 윤충류의 분류학적 연구를 위하여 1990년 3月부터 1992년 2월까지 전국 일대에 산재해 있는 댐, 호수, 저수지, 연못, 논, 웅덩이 등을 대상으로 총 205개 지점에서 채집을 실시하여 조사한바, Brachionidae과의 Brachionus속에 9아종 2변종 6형이, 그리고 Platyas속에는 1종이 밝혀졌는데, 그중 Brachionus속의 1아종 4형 : Brachionus urceolaris bennini, B. angularis f. bidens, B. quadridentatus f. rhenanus, B. forficuIa f. minor, B. forficula f. angularis가 한국 미기록으로 판명되어 기재와 함께 도판을 작성하였고, 본 연구에서 확인된 Brachionus와 Platyas속내의 종들에 대해서는 물론, 연차적인 발표를 목적으로 한국산 윤형동물에 대한 전체척언 검색표를 만들었다. 따라서 지금까지 기록된 한국산 윤충류는 총 2강 4목 14과 40속 134종, 15아종, 9변종, 9형이 된다.

• Asian-Australasian Journal of Animal Sciences
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• 제13권7호
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• pp.888-893
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• 2000

The present study was conducted to evaluate heterosis effects of body weight and jumping height for successive generations of rotational crossing using two subspecies of mice which are very different in body weight and in genetic relationship from each other. Domesticated laboratory mouse $CF_{{\sharp}1}$ (C) and Yonakuni wild mouse (Y) were used as materials. Two groups of rotational crossing was made according to the parent used at the beginning of crosses, C male$\times$Y female and Y male$\times$C female. These crosses were done to produce the first ($G_1$ and $G_1{^{\prime}}$), second ($G_2$ and $G_2{^{\prime}}$) and third generations ($G_3$ and $G_3{^{\prime}}$) with sire used was alternated. Individual body weights were weighed at 1 (wk1), 3 (wk3), 6 (wk6) and 10 weeks of age (wk10) and jumping heights were measured at six weeks of age (wk6). Only the first litter used. For body weight, results of this study showed that genetic group effects were significant (p<0.01) source of variation at all ages studied. Sex effects were significant (p<0.01) at wk3, wk6 and wk10, but not at wk1. Significant interaction effects (p<0.01) between genetic group and sex were found at wk6 and wk10. The C mice with large maternal effects produced heavier offspring body weight and crosses using sire of this subspecies maintained heavy weight compared to wild Y mouse sire that has small body size. Heterosis tended to exist at the rotational crossing started from Y male C female. For jumping height, effects of genetic group and sex were significant, sire and dam effects (heterosis) exhibited from the first to third generations, and no maternal effects were observed.

• 한국육종학회지
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• 제41권2호
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• pp.101-105
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• 2009

전 세계에서 광범위하게 수집한 벼 유전자원 320개를 63개의 아종특이적마커로 분석하여 유전자원의 다양성, 유연관계 및 유전집단의 구조분석을 하여 아종특이적마커의 아종판별 효율을 검정하고 아종의 게놈 구성을 검토하고자 본 시험을 수행하였다. 1. 본 연구에서 사용한 63개의 아종특이적 마커는 벼 품종을 인디카와 자포니카 두 아종으로 구분하는데 효과적으로 이용할 수 있었다. 2. 실험에 사용한 320개의 벼 유전자원들은 자포니카군(128개)과 인디카군(178개)으로 나눌 수 있었고 Basmati370, Shennong27 등 14개 품종은 중간형 admixture형태의 품종으로 구분할 수 있었다.

• Journal of Microbiology and Biotechnology
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• 제29권5호
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• pp.785-793
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• 2019

Black rot caused by Xanthomonas campestris pv. campestris (Xcc) is the most damaging disease in Brassica crops around the world. In this study, we developed a molecular marker specific to Xcc race 5. To do this, the available whole genome sequences of Xcc races/strains and Xc subspecies were aligned and identified a highly variable genomic region (XccR5-89.2). Subsequently, a primer set covering the 'XccR5-89.2' region was designed and tested against the genomic DNA of Xcc races/strains, Xc subspecies and other plant-infecting bacterial strains (Pseudomonas syringae pv. maculicola and Erwinia carotovora subsp. carotovora). The results showed that the 'XccR5-89.2' primer pair amplified a 2,172-bp fragment specific to Xcc race 5. Moreover, they also amplified a 1,515-bp fragment for Xcc race 1 and an over 3,000-bp fragment for Xcc race 3. However, they did not amplify any fragments from the remaining Xcc races/strains, subspecies or other bacterial strains. The 'XccR5-89.2' primer pair was further PCR amplified from race-unknown Xcc strains and ICMP8 was identified as race 5 among nine race-unknown Xcc strains. Further cloning and sequencing of the bands amplified from race 5 and ICMP8 with 'XccR5-89.2' primers revealed both carrying identical sequences. The results showed that the 'XccR5-89.2' marker can effectively and proficiently detect, and identify Xcc race 5 from Xcc races/strains, subspecies and other plant-infecting bacteria. To our knowledge, this is the first report for an Xcc race 5-specific molecular marker.

• Animal Systematics, Evolution and Diversity
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• 제38권4호
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• pp.226-237
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• 2022

Arcuospathidium is a haptorian ciliate genus composed of 18 species, and only one species has been reported in Korea. Here, we identify two unrecorded Arcuospathidium subspecies by morphological observation of both living and protargol-impregnated specimens with the small subunit ribosomal RNA (18S rRNA) gene sequence. These subspecies, Arcuospathidium cultriforme cultriforme (Penard, 1922) Foissner, 1984 and A. cultriforme scalpriforme (Kahl, 1930) Foissner, 2003, were isolated from various terrestrial habitats in July and August 2013, respectivley. Arcuospathidium cultriforme cultriforme is similar to A. cultriforme scalpriforme by a knife-shaped body, a twisted-shaped macronucleus, number of dorsal brushes, position of dorsal brushes, and shape of macronucleus but former mainly differs from the body length to oral bulge length ratio (27-38% vs. 41-53%), extrusome (one types vs. three types), cyst shape (roughly faceted wall vs. smooth surface and thin wall) and number of somatic kinety rows(18-30 vs. 30-44). Additionally, we analyzed the 18S rRNA gene sequences of two A. cultriforme subspecies and compared them with the sequences from GenBank to confirm their identification at the molecular level. As the results of genetic analysis, the 18S rRNA gene sequence of the Korean A. cultriforme cultriforme population is most similar to that of Austrian population. Also, the sequence of the Korean A. cultriforme scalpriforme population is most similar to that of another population with some nucleotide differences.