Browse > Article
http://dx.doi.org/10.5635/ASED.2022.38.4.027

New Records of Two Arcuospathidium Subspecies (Ciliophora: Haptoria: Arcuospathidiidae) from Korea  

Jang, Seok Won (Protist Research Team, Nakdonggang National Institute of Biological Resources)
Nam, Seung Won (Protist Research Team, Nakdonggang National Institute of Biological Resources)
Shazib, Shahed Uddin Ahmed (Department of Biological Science, College of Natural Sciences, University of Ulsan)
Shin, Mann Kyoon (Department of Biological Science, College of Natural Sciences, University of Ulsan)
Publication Information
Animal Systematics, Evolution and Diversity / v.38, no.4, 2022 , pp. 226-237 More about this Journal
Abstract
Arcuospathidium is a haptorian ciliate genus composed of 18 species, and only one species has been reported in Korea. Here, we identify two unrecorded Arcuospathidium subspecies by morphological observation of both living and protargol-impregnated specimens with the small subunit ribosomal RNA (18S rRNA) gene sequence. These subspecies, Arcuospathidium cultriforme cultriforme (Penard, 1922) Foissner, 1984 and A. cultriforme scalpriforme (Kahl, 1930) Foissner, 2003, were isolated from various terrestrial habitats in July and August 2013, respectivley. Arcuospathidium cultriforme cultriforme is similar to A. cultriforme scalpriforme by a knife-shaped body, a twisted-shaped macronucleus, number of dorsal brushes, position of dorsal brushes, and shape of macronucleus but former mainly differs from the body length to oral bulge length ratio (27-38% vs. 41-53%), extrusome (one types vs. three types), cyst shape (roughly faceted wall vs. smooth surface and thin wall) and number of somatic kinety rows(18-30 vs. 30-44). Additionally, we analyzed the 18S rRNA gene sequences of two A. cultriforme subspecies and compared them with the sequences from GenBank to confirm their identification at the molecular level. As the results of genetic analysis, the 18S rRNA gene sequence of the Korean A. cultriforme cultriforme population is most similar to that of Austrian population. Also, the sequence of the Korean A. cultriforme scalpriforme population is most similar to that of another population with some nucleotide differences.
Keywords
Arcuospathidiidae; Haptorian ciliates; morphology; protargol impregnation; 18S rRNA gene;
Citations & Related Records
Times Cited By KSCI : 11  (Citation Analysis)
연도 인용수 순위
1 Choi JM, Jung JH, Kim YO, 2018. First record of Amphisiella milnei (Ciliophora, Stichotrichida) from Korea. Animal Systematics, Evolution and Diversity, 34:143-151. https://doi.org/10.5635/ASED.2018.34.3.143   DOI
2 Dragesco J, Dragesco-Kerneis A, 1979. Cilies muscicoles nouveaux ou peu connus. Acta Protozoologica, 18:401-416.
3 Foissner W, 1996. Terrestrial ciliates(Protozoa, Ciliophora) from two islands(Gough Marion) in the southern oceans, with description of two new species, Arcuospathidium cooperi and Oxytricha ottowi. Biology and Fertility of Soils, 23:282-291.   DOI
4 Foissner W, 1999. Notes on soil ciliate biota (Protozoa, Ciliophora) from the Shimba Hills in Kenya (Africa): diversity and description of three new genera and ten new species. Biodiversity and Conservation, 8:319-389.   DOI
5 Foissner W, 2003b. Two remarkable soil spathidiids(Ciliophora: Haptorida), Arcuospathidium pachyoplites sp. n. and Spathidium faurefremieti nom. n. Acta Protozoologica, 42:145-159.
6 Foissner W, Agatha S, Berger H, 2002. Soil ciliates (Protozoa, Ciliophora) from Namibia (Southwest Africa) with emphasis on two contrasting environments, the Etosha region and the Namib Desert. Denisia, 5:1-1459.
7 Foissner W, Foissner I, 1988. The fine structure of Fuscheria terricola Berger et al., 1983 and a proposed new classification of the subclass Haptoria Corliss, 1974 (Ciliophora, Litostomatea). Archiv fur Protistenkunde, 135:213-235. https://doi.org/10.1016/S0003-9365(88)80070-8   DOI
8 Foissner W, Xu K, 2007. Monograph of the Spathidiida (Ciliophora, Haptoria). Vol. I: Protospathidiidae, Arcuospathidiidae, Apertospathulidae. Monographiae Biologicae, Vol. 81. Springer, Dordrecht, pp. 1-486.
9 Jang SW, Omar A, Nam SW, Jung JH, 2022. Morphology and molecular phylogeny of Fuscheriides baugilensis sp. nov. (Protozoa, Ciliophora, Haptorida) from South Korea. Diversity, 14:70. https://doi.org/10.3390/d14020070   DOI
10 Jang SW, Vd'acny P, Shazib SUA, Shin MK, 2015. Morphology, ciliary pattern and molecular phylogeny of Trachelophyllum brachypharynx Levander, 1894 (Litostomatea, Haptoria, Spathidiida). Acta Protozoologica, 54:123-135. https://doi.org/10.4467/16890027AP.15.010.2735   DOI
11 Jung JH, 2021b. Taxonomy of four scuticociliates(Protozoa: Ciliophora) from coastal waters of South Korea. Journal of Species Research, 10:184-190. https://doi.org/10.12651/JSR.2021.10.2.184   DOI
12 Jung JH, Yeo JH, 2020. New record of five ciliate species from temporary ponds on a grass lawn. Journal of Species Research, 9:68-76. https://doi.org/10.12651/JSR.2020.9.1.068   DOI
13 Kahl A, 1930a. Neue und erganzende Beobachtungen holotricher Infusorien. II. Archiv fur Protistenkunde, 70:313-416.
14 Kahl A, 1930b. Urtiere oder Protozoa. I: Wimpertiere oder Ciliata (Infusoria) 1. Allgemeiner Teil und Prostomata. Tierwelt Deutschlands, 18:1-180.
15 Kim JH, Jung JH, 2019. Brief description of newly recorded eight ciliate species (Protozoa, Ciliophora) collected from South Korea. Journal of Species Research, 8:380-388. https://doi.org/10.12651/JSR.2019.8.4.380   DOI
16 Kim JH, Omar A, Jung JH, 2020a. Brief description of 18 newly recorded ciliate species from soil and inland waters(Protozoa, Ciliophora) in South Korea. Journal of Species Research, 9:251-268. https://doi.org/10.12651/JSR.2020.9.3.251   DOI
17 Park MH, Min GS, 2019. New records of one marine and two soil ciliates (Ciliophora: Intramacronucleata) from Korea. Journal of Species Research, 8:113-115. https://doi.org/10.12651/JSR.2019.8.1.113   DOI
18 Kumar S, Stecher G, Li M, Knyaz C, Tamura K, 2018. MEGA X: molecular evolutionary genetics analysis across computing platforms. Molecular Biology and Evolution, 35:1547-1549. https://doi.org/10.1093/molbev/msy096   DOI
19 Kwon CB, Shin MK, Kim SY, Kim SJ, Kim YO, Jung JH, 2019. Phylum CILIOPHORA. In: National species list of Korea, II. Vertebrates, invertebrates, protozoans(Ed., National Institute of Biological Resources). Designzip, Seoul, pp. 673-697 (in Korean).
20 Omar A, Jung JH, 2021. New record of 21 ciliate species(Protozoa, Ciliophora) from South Korea. Journal of Species Research, 10:301-320. https://doi.org/10.12651/JSR.2021.10.3.301   DOI
21 Penard E, 1922. Etudes sur les Infusoires d'Eau Douce. Georg and Cie, Geneve, pp. 1-331.
22 Wilbert N, 1975. Eine verbesserte Tecknik der Protargolimpragnation fur Ciliaten. Mikrokosmos, 64:171-179.
23 Yildiz I, 2018. Morphology and phylogeny of Apertospathula oktemae n. sp. (Ciliophora, Haptoria, Sapthidiida) from Lake Van, Turkey. European Journal of Protistology, 66:1-8. https://doi.org/10.1016/j.ejop.2018.06.001   DOI
24 Kabir AS, Bharti D, Shin MK, 2018. Redescription of Australocirrus shii and first report of Afrokeronopsis aurea (Ciliophora: Spirotrichea: Sporadotrichida) from South Korea. Animal Systematics, Evolution and Diversity, 34:37-49. https://doi.org/10.5635/ASED.2018.34.1.024   DOI
25 Chae KS, Kim KS, Min GS, 2021. Redescription of two soil ciliates, Anteholosticha bergeri and Bakuella granulifera, from South Korea. Journal of Species Research, 10:63-71.   DOI
26 Foissner W, 1984. Infraciliatur, Silberliniensystem und Biometrie einiger neuer und wenig bekannter terrestrischer, limnischer und mariner Ciliaten (Protozoa: Ciliophora) aus den Klassen Kinetofragminophora, Colpodea und Polyhymenophora. Stapfia, 12:1-165.
27 Foissner W, 2003a. Cultellothrix velhoi gen. n., sp. n., a new spathidiid ciliate (Ciliophora: Haptorida) from a Brazilian floodplain soil. Acta Protozoologica, 42:47-54.
28 Foissner W, Berger H, Xu K, Zechmeister-Boltenstern S, 2005. A huge, undescribed soil ciliate (Protozoa: Ciliophora) diversity in natural forest stands of Central Europe. Biodiversity and Conservation, 14:617-701. https://doi.org/10.1007/s10531-004-3923-6   DOI
29 Foissner W, Wolf KW, Kumar S, Xu K, Quintela-Alonso P, 2014. Five new spathidiids (Ciliophora: Haptoria) from Caribbean tank bromeliads. Acta Protozoologica, 53:159-194. https://doi.org/10.4467/16890027AP.14.015.1596   DOI
30 Jung JH, 2021a. New record of two Oligohymenophorean ciliates(Protozoa: Ciliophora) from Korea. Animal Systematics, Evolution and Diversity, 37:280-286. https://doi.org/10.5635/ASED.2021.37.4.029   DOI
31 Pan X, Bourland WA, Song W, 2013. Protargol synthesis: an inhouse protocol. Journal of Eukaryotic Microbiology, 60:609-614. https://doi.org/10.1111/jeu.12067   DOI
32 Kearse M, Moir R, Wilson A, Stones-Havas S, Cheung M, Sturrock S, Buxton S, Cooper A, Markowitz S, Duran C, Thierer T, Ashton B, Mentjies P, Drummond A, 2012. Geneious Basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics, 28:1647-1649. https://doi.org/10.1093/bioinformatics/bts199   DOI
33 Kim JH, Omar A, Moon JH, Jung JH, 2020b. Taxonomy of 16 indigenous ciliate species(Protozoa, Ciliophora) from South Korea. Journal of Species Research, 9:427-442. https://doi.org/10.12651/JSR.2020.9.4.427   DOI
34 Lynn DH, 2008. The ciliated protozoa: characterization, classification, and guide to the literature. Springer, New York, pp. 1-605.
35 Xu K, Foissner W, 2005. Morphology, ontogenesis and encystment of a soil ciliate (Ciliophora, Haptorida), Arcuospathidium cultriforme (Penard, 1922), with models for the formation of the oral bulge, the ciliary patterns, and the evolution of the spathidiids. Protistology, 4:5-55.