• Title/Summary/Keyword: subsistence

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Induction of Apoptosis by Samgibopae-tang in Human Non-small-cell Lung Cancer Cells (인체폐암세포 NCI-H460 및 A549의 apoptosis 유발에 미치는 삼기보배탕의 영향)

  • Heo, Man-Kyu;Heo, Tae-Yool;Kim, Ki-Tak;Byun, Mi-Kwon;Kim, Jin-Young;Sim, Sung-Heum;Kim, Koang-Lock;Kam, Cheol-Woo;Park, Dong-Il
    • The Journal of Internal Korean Medicine
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    • v.28 no.3
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    • pp.473-491
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    • 2007
  • Objectives : This study was designed to investigate the antiproliferative activity of the water extract of Samgibopae-tang (SGBPT) in NCI-H460 and A549 non-small-cell lung cancer cell lines Methods : In this study, we measured the subsistence, form of NCI-H460 and A549 non-small-cell lung cancer cell by hemocytometer and DAPI staining. In each cell, we analyzed DNA fragmentation. reverse transcription-polymerase chain reaction and measured activity of caspase-3, caspase-8 and caspase-9. Results and Conclusions : We found that exposure of A549 cells to SGBPT resulted in growth inhibition in a dose-dependent manner. butSGBPT did not affect the growth of NCI-H460 cells. The antiproliferative effect by SGBPT treatment in A549 cells was associated with morphological changes. SGBPT treatment partially induced the expression of DR5 cells and the expression of Faswas markedly increased in both transcriptional and translational levels in A549 cells. SGBPT treatment partially induced the expression of Bcl-2, Bcl-XL and the expression of Bid was markedly decreased in translational levels in A549 cells. However, SGBPT treatment did not affect the expression of IAP family in A549 orNCI-H460 cells. SGBPT treatment partially induced the expression of caspase-3, caspase-8, caspase-9 activity which markedly increased in a dose-dependent manners in A549 cells. The fragmental development of PARP and ${\beta}$-catenin protein was observed in A549 cells by SGBPT treatment. SGBPT treatment induced the expression of PLC-${\gamma}1$ protein which decreased in A549 cells. SGBPT treatment partially induced the expression of DFF45/ICAD which markedly increased in a dose-dependent manner in A549 cells. Taken together. these findings suggested that SGBPT-induced inhibition of human lung carcinoma did not affect NCI-H460 cell growth. However, SGBPT-induced inhibition of human lung carcinoma A549 cell growth was associated with the induction of death receptor and mitochondrial pathway. The results provided important new insights into the possible molecular mechanisms of the anti-cancer activity of SGBPT.

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Selection of Superior Sorghum Accession by Assessing Agronomic Characters and Biological Activity (수수 수집종의 농업적 형질 및 생리활성 분석을 통한 우수계통 선발)

  • Jeon, Mi Ran;Yoo, Ji Hye;Kim, Chang Heum;Choi, Jae Hoo;Kang, Byeong Ju;Seong, Eun Soo;Yu, Chang Yeon
    • Korean Journal of Medicinal Crop Science
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    • v.24 no.5
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    • pp.386-392
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    • 2016
  • Background: Sorghum is a major cereal food crop used in many parts of the world. It has been grown on a subsistence level by farmers, under various conditions of environmental stresses in the semi-arid tropics of Africa and Asia. This plant has received significant attention because of its ability to reduce cholesterol in the blood, and its anti-dementia, antioxidant, and antimicrobial properties. It is possible to develop a functional and commercially viable sorghum variety by using superior cultivars of sorghum. The objective of this study was to build a database of superior sorghum accession. Methods and Results: We used 250 sorghum accessions collected from different geographical bioregions in Korea. We determined various agronomic characters including germination rate and ear length of these accessions. To determine the antioxidant capacity, we measured the 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity, 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)-diammonium salt radical scavenging activity, total phenolic contents, and total flavonoid contents. Accession 189 showed higher germination (> 80%) than the other accessions. Higher 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity was observed in 11-SB-078 ($RC_{50}$; $1.89{\pm}2.88{\mu}g/m{\ell}$), and higher 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)-diammonium salt radical scavenging activity was recorded in 11-SB-116 ($RC_{50}$; $35.48{\pm}2.42{\mu}g/m{\ell}$) than in the other accessions. The ear length ranged from 15 cm to 48 cm, the total phenolic contents ranged from $3mg{\cdot}GAE/g$ to $77mg{\cdot}GAE/g$, and total flavonoid contents ranged from $0.09mg{\cdot}QE/g$ to $1.07mg{\cdot}QE/g$. Conclusions: Among 250 sorghum accessions, we selected 10 with both superior agronomic characters and highly functional food quality.

Consequences of Water Induced Disasters to Livelihood Activities in Nepal

  • Gurung, Anup;Karki, Arpana;Karki, Rahul;Bista, Rajesh;Oh, Sang-Eun
    • Korean Journal of Environmental Agriculture
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    • v.31 no.2
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    • pp.129-136
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    • 2012
  • BACKGROUND: The changes in the climatic conditions have brought potentially significant new challenges, most critical are likely to be its impact on local livelihoods, agriculture, biodiversity and environments. Water induced disasters such as landslides, floods, erratic rain etc., are very common in developing countries which lead to changes in biological, geophysical and socioeconomic elements. The extent of damages caused by natural disasters is more sever in least developing countries. However, disasters affect women and men differently. In most of the cases women have to carry more burden as compared to their male counterpart during the period of disasters. METHODS AND RESULTS: This study examines the impact of disasters on the local livelihood especially agriculture and income generating activities of women in three districts of Nepal. The study uses the primary data collected following an exploratory approach, based on an intensive field study. The general findings of the study revealed that women had to experience hard time as compared to their male counterpart both during and after the disaster happen. Women are responsible for caring their children, collecting firewood, fetching water, collecting grass for livestock and performing household chores. Whereas, men are mainly involved in out-migration and remained out-side home most of the time. After the disaster occurred, most of the women had to struggle to support their lives as well as had to work longer hours than men during reconstruction period. Nepal follows patriarchal system and men can afford more leisure time as compared to women. During the disaster period, some of the households lost their agricultural lands, livestock and other properties. These losses created some additional workload to women respondent, however at the same time; they learn to build confidence, self-respect, self-esteem, and self-dependency.Although Nepal is predominantly agriculture, majority of the farmers are at subsistence level. In addition, men and women have different roles which differ with the variation in agro-production systems. Moreover women are extensively involved in agricultural activities though their importances were not recognized. Denial of land ownership and denial of access to resources as well as migration of male counterparts are some of the major reasons for affecting the agricultural environments for women in Nepal. CONCLUSION: The shelter reconstruction program has definitely brought positive change in women's access to decision making. The gradual increase in number of women respondent in access to decision making in different areas is a positive change and this has also provided them with a unique opportunity to change their gendered status in society.Furthermore, the exodus out-flow of male counterparts accelerated the additional burden and workload on women.

A Comparative Analysis of Major Oil Spill Compensation Systems in France, Spain, and Korea - In the Case of M/T Erika, Prestige, and Hebei Spirit - (프랑스.스페인.한국의 대형유류오염손해배상제도에 관한 비교연구 -에리카호.프레스티지호.허베이스피리트호를 중심으로-)

  • Cho, Dong-Oh;Mok, Jin-Yong
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.14 no.3
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    • pp.177-181
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    • 2008
  • In this study, the authors analyzed the limit of IOPC Fund system, such as limit of compensation, amount of FC, long period of assessment by IOPC Fund, issue of non verification by poor claimant or small business, issue of poor claimant subsistence in early stage of accidents, and comparatively analyzed how the three countries resolved these limits by enacting special laws and government policy. Until recently most governments have carried out prevention policy for maritime safety, oil spill response, investigation of maritime accidents, and restoration of marine environment when oil spill accidents happened. However, governments have not actively participated in the oil spill compensation process because it is a matter of private sectors between the polluter and claimant. The governments have only limited their role in ratifying Civil Liability Convention and Fund Convention and enacted relevant domestic laws. However, the governments of France, Spain, and Korea have actively participated in the compensation process of oil pollution incidents of M/T Erika, M/T Prestige, and M/T Hebei Spirit. This is because they had experiences of unsuccessful compensation in M/T Amoco Cadiz, M/T Agean Sea, and M/T Sea Prince.

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Constructing of Humidity Automatic Regulation Environment to Build Effective Mushroom Growing Environment (버섯의 효과적인 생육환경 구축을 위한 자동 습도조절 환경 연구)

  • Xu, Chen-Lin;Lee, Hyun-Chang;Kang, Sun-kyung;Shin, Seong-Yoon
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.19 no.11
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    • pp.2597-2602
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    • 2015
  • With the development of economy and improving of people's living standards, people dietary needs will be achieved from subsistence to high nutrition and from high nutrition to healthy transformation. Mushroom as a kind of highly nutritious, low fat, rich vitamin food has a great interest among the people. This makes the mushroom into a new sunrise industry and it gradually from pure manual cultivation develops toward the fully automatic factory. In the process of mushroom factory production, regulation of environmental factors directly affects the yield and quality of mushroom. In related to the methods of mushroom cultivation, the recent technologies apply the new technology such as sensors and IT convergence services. And then cultivating mushroom is managed effectively. This paper in order to solve the above problems and construct an effective mushroom growth environment using technology such as humidity sensor construct an environment that can automatically adjust the humidity. This environment has important significance to improve the level of automation mushroom production, increase yield per unit area and quality of mushroom, increase economic efficiency of mushroom production, and enhance the competitiveness of mushroom production.

Archaeogenetic Research of Excavated Human Bones from the Ancient Tombs (분묘 유적지 출토 인골에 대한 고고유전학 연구)

  • Jee, Sang Hyun;Chung, Yong Jae;Seo, Min Seok
    • Korean Journal of Heritage: History & Science
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    • v.41 no.1
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    • pp.99-108
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    • 2008
  • The paleogenetic analysis has become an increasingly important subject of archaeological, anthropological, biological as well as public interest. Recently, scientific research for human skeletal remains was more activated because of increasing awareness of the valuable archaeological information by the ancient DNA analysis. State of preservation of organic remains vary in different soil and burying environmental condition. Almost all available tissue disappear to analysis ancient DNA of bone in acidic soil caused by climate and geological features in Korea. Many preserved human remains excavated in the 'Heogwakmyo'(limelayered tomb of Chosun Dynasty Period) is able to explain through the relationship between burial conditions and bone survival form the burial method and ceremony. Ancient DNA analysis of excavated human bone form ancient tomb requires to remove contaminants such as microorganism's DNA and soil components that affect authentic results. Particularly, contamination control of contemporary human DNA is major serious problem and should verified by criteria of authenticity. In order to understand migration and culture of ancient population, when possible, ancient DNA studies needs to go abreast both radiocarbon and stable isotope studies because the dietary inferences will suggest ancient subsistence and settlement patterns. Also when the paleogenetic research supported with the arts and humanities research such as physical anthropology and archaeology, more valuable ancient genetic information is providing a unique results about evolutionary and population genetics studies to reconstruct the past.

Cultivation of millet in Mahan-Baekje Villages: Archaeobotanical examination of Osan site, Jangheung, Korea (전남 마한백제계 취락의 서속(黍粟) 재배와 농경 체계: 장흥 봉림리 오산유적 자료를 중심으로)

  • RYU Ara;KIM Sebin;OH Byungwook;KIM Minkoo
    • Korean Journal of Heritage: History & Science
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    • v.56 no.2
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    • pp.48-60
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    • 2023
  • Although foxtail and broomcorn millet have been cultivated since the Neolithic Age on the Korean Peninsula, there have been few studies on how the importance of millet cultivation changed over time. The discovery of millet has been rare in the Mahan-Baekje villages in the Jeonnam region, and archaeological discussions on ancient farming have centered around rice farming. However, a large amount of millet was found at the Osan site in Bongrim-ri, Jangheung, showing that the tradition of millet cultivation continued during the AD 3-4 centuries. In contrast to rice farming, which requires low-lying wetlands, hot and humid summers, and a large labor force, millet cultivation has few restrictions in terms of growing environment and labor mobilization. Regarding harvesting seasons, the crops at the Osan site can be divided into fall harvesting (rice, legumes, millet) and summer harvesting (wheat). Regarding field locations, the crops are divided into wetland crops (rice) and dryland crops (wheat, legumes, millet). The operation of the dual agricultural system contributed to subsistence resilience and agricultural production, making it possible to effectively manage social and climatic crises.

Effects of Strength Recognition on the Intent of Start-up after Retirement: To the Office Workers (강점인식이 은퇴 후 창업의도에 미치는 영향: 직장인을 대상으로)

  • Park, Hye sung;Park, Woojin;Yun, Bae byung
    • Asia-Pacific Journal of Business Venturing and Entrepreneurship
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    • v.15 no.1
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    • pp.143-150
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    • 2020
  • This research is intended to identify how office worker's recognition of strength affects the intention of starting a business when it is used as a medium for utilizing strength. The survey period was conducted for two months from January 17, 2019 to March 8, 2019. In order to achieve the object of this study, a significant number of employees have been collected nationwide, and 287 office workers were analyzed after collecting 300 questionaires, excluding 13 office workers who were not sincere. The significant data were analyzed by utilizing the structural equation and technical statistics and by verifying the conformity of structural model and measurement model and by investigating the relationship between variables. The results of this study are summarized as follows: First, employee's perception of strength has been found to have a significant effect on the utilizing of strength. Second, the use of workers' strengths has been found to have a significant effect on the intention of starting a business. Third, it has been confirmed that in the relationship between the employee's perception of strength and intention to start a business, the recognition of strength has a significant effect on the intention of starting a business only through the use of strength. Most office workers are nervous about the future and make various plans. The time to live after retirement is getting longer and jobs and working hours are shrinking at an alarming rate. According to the analysis on the status of small business owners in the mid-term of 2017 data, the average monthly operating profit of small business owners across the country is decreasing over the years, and most start-ups are subsistence-type start-ups, and they decide to start their own businesses because they have no other choice than start-ups. As the number of small business owners suffering from financial difficulties has increased since the financial crisis and insecurity of job is pushing those with financial difficulties after retirement to the battleground of the start-up market, the result of this research suggest that the workers need to recognize their own strength and enhance the importance of utilizing the strength.

A Study on the Regionality of Land-Lease Farming : A Comparative Analysis of the Case Study Areas (임차농(賃借農)의 지역성(地域性)에 관한 연구 -사례지역의 비교분석-)

  • Suh, Chan-Ki
    • Journal of the Korean association of regional geographers
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    • v.3 no.2
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    • pp.121-150
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    • 1997
  • This Study puts the purpose to explicate the regionalities of land-lease farming by a comparative analisis of the five case study areas in Kungpook Province as the agricultural space system of Teagu metropolitan city. For the regional comparative analysis the province was divided into the three zones with the distance from the central city(Teagu): urban fringes, intermediate and remote zone, and then subdivided into the five regions by farming systems : horticulture, rice-farming, and dry-field farming. The data were collected from 77 land-leasing farmers by questionnaire and interview with farm managers in 5 sample villages representing the regionalities of the above five regions respectively. In spite of relatively restricted scope of the research areas, the analytical results appear remarkable regional differences in the characteristics of land-lease farming within a single agricultural space system. In the final analysis the regionalities of the five land-lease farming regions could be described respectively as follows. (1) Koryong-Gun in the inner urban fringe zone : The developing land-lease farming region of commercialized suburban horticulture with medium scale. (2) Songju-Gun in the outer urban fringe zone : The developing land-lease farming region of highly commercialized horticulture with large scale. (3) Uisong-Gun in the intermediate zone : The stagnated land-lease farming region of commercialized rice-farming with large scale. (4) Yongil-Gun in the intermediate zone : The stagnated land-lease farming region of commercializing dry-field farming with medium scale. (5) Ponghwa-Gun in the remote zone : The stagnated and delayed region in commercializing of intermountain dry-field land-lease farming with small scale. These varied regionalities resulted from the diverse spatiality as a complex of spatial orders and localities. The spatial orders in this study are frequently recognizable as a form of distance-decay, and the locality of a region is determined mostly by the its peculiarity of physical and population conditions. In the comparative analysis of the regionalities the degree of commercialization of a region is a most comprehensive and useful frame of reference because it reflects the degree of development of capitalist land-lease farming. Finally these apparent regional differentiations of land-lease farming within a agricultural space system raise the problem of impracticality of the existing uniform logic on the land-lease farming such as "large scale farms share larger part of leased farmland." This problem suggests the urgent need of reappraisal of many aspatial logics and theories on the land-lease farming.

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Cultivational Possibilities of Camellia sinensis L. in the Mountain-area of West-Gyeongnam Province, Korea (경남 서부 산간지의 차나무 (Camellia sinensis L.) 재배 가능성)

  • Lee Seong-Tae;Shon Gil-Man;Kang Jin-ho;Lee Yong-Ho
    • Korean Journal of Plant Resources
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    • v.18 no.1
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    • pp.1-7
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    • 2005
  • This study was conducted to investigate the possibility of cultivating tea plants planted in the mountain area of west Gyeongnam province, Korea for 2 years from March 27th, 2001 to July 30th 2003. Ninety each plants collected from 5 different sife were cultivated in nursery cup pot$(\phi\;16cm)$. in the greenhouse condition and transplanted in 5 different location and monitored their survival growing state etc. The results obtained are as follow : 1. Survival rate tea plants after transfer to soil was relatively high Gaya-myeon Hanyang-gun with $90.0\%$ and the lowest in Buksang-meyon Geochang-gun with $80.0\%$. The tea plant collected from Gaya-myeon showed the best growing activity at early stage. 2. For the second year of harvesting time, survival rate was the highest in Machun-myeon Hamyang-gun with $92.2\%$ and the lowest was Buksang-myeon Geochang-gun with $76.7\%$. 3. For the 3rd year of harvesting time, it was impossible to data collection because of the most upper parts of plants were killed by severe freezing weather condition. In the Baekjeon-myeon, Hamyang-gun Buksang-myeon, Ungyang-myeon Geochang-gun, which are severly cold$(below\;-10^{\circ}C)$ in winter season, seems not a suitable places for tea plant cultivation since it is very different to harvest the young loaves in growing season. In conclnsion we could select two sites Gaya-myeon, Hapcheon-gun, Machun-myeon, Hamyang-gun, as tea plant cultivation in the mountain area of west-Gyeongnam province, korea.