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Fiber-optic fluoroimmunosensor for foodborn pathogens using an optical evanescent field (광섬유 소산장을 이용한 식중독균 신속검출용 형광면역센서)

  • Yeom, Se-Hyuk;Park, Chang-Sub;Kim, Do-Eok;Kim, Kyu-Jin;Kang, Byoung-Ho;Kang, Shin-Won
    • Journal of Sensor Science and Technology
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    • v.16 no.6
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    • pp.441-448
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    • 2007
  • In this study, the fiber-optic fluoro-immunosensor was designed to detect foodborne pathogens. The fabricated system is composed of the multimode optical fiber on which antibodies are immobilized. Then, a sandwich immunoassay is applied to the fabricated the fiber-optic fluoro-immunosensor. In the "sandwich" binding format, a primary or "capture" antibody is immobilized on the core surface of the multimode optical fiber and a secondary or named as "tracer" antibody is added to the bulk solution. A tracer is labeled FITC (fluorescein isothiocyanate; ${\lambda}ex$=492 nm, ${\lambda}em$= 520 nm). Different concentrations of antigens are tested in different fibers. The detection limit of the fabricated system is 5.08×103 cfu/ml for Vibrio antigen and $0.1{\mu}g/ml$, $0.05{\mu}g/ml$ in non-labeled monolayer phosphate buffered saline (NMP), non-labeled monolayer carbonate bicarbonate buffer (NMC), respectively.

The Effect of Modifying and Applying Waste Management Program on Environmental Sensitivity and Environmental Conservation Behavior (폐기물 관리 프로그램의 구안과 적용이 환경 감수성과 환경보전 행동에 미치는 영향)

  • Kim, Jung-Hee;Nam, Young-Sook
    • Hwankyungkyoyuk
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    • v.18 no.3 s.28
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    • pp.1-18
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    • 2005
  • In the sustainable waste management, the necessity of environmental education which improves acquisition of the knowledge, attitudes and values for the environment is being emphasized to help students make environmentally desirable decision and promote responsible environmental behavior. However, the environmental education in high schools, as a whole, would be susceptible to many problems caused by tile educational administration focusing only on the college entrance examination. Therefore, this study modifies and applies a program using four teaching/learning methods (Investigation study, Internet-based learning, Field work, Issue analysis study) which deals with the sustainable waste management as the learning subject for the environmental education, and tries to find its effects on the students' environmental sensitivity and environmental conservation behavior in high schools. The main results of this study are summarized as fellows. First, the waste management program had little influence on the environmental sensitivity of experimental groups, while it had positive effect on their improvement of the environmental conservation behavior. Second, there were little meaningful effect on general environmental conservation behavior, but there were some significant results to environmental conservation behavior related to the sustainable waste management. In addition, positive results were found in ecomanagement and consumerism, whereas there were no meaningful results about in persuasion and legal action, which were sub-domains of the waste management program. Among the waste management programs, the school waste investigation study had the most positive effect on the students' recognition and solution of environmental problems as well as improvement of environmental conservation behavior. The easiest one of the four teaching/learning methods was the Internet-based learning, while the most difficult one of them was the issue analysis study. Consequently, in order to achieve the goals of the environmental education in the academic field, there has to be selected the most suitable one of the teaching/learning methods and to be applied to the class, according to the detailed objectives and loaming contents for the environmental education.

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An Improved Genetic Algorithm for Integrated Planning and Scheduling Algorithm Considering Tool Flexibility and Tool Constraints (공구유연성과 공구관련제약을 고려한 통합공정일정계획을 위한 유전알고리즘)

  • Kim, Young-Nam;Ha, Chunghun
    • Journal of Korean Society of Industrial and Systems Engineering
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    • v.40 no.2
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    • pp.111-120
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    • 2017
  • This paper proposes an improved standard genetic algorithm (GA) of making a near optimal schedule for integrated process planning and scheduling problem (IPPS) considering tool flexibility and tool related constraints. Process planning involves the selection of operations and the allocation of resources. Scheduling, meanwhile, determines the sequence order in which operations are executed on each machine. Due to the high degree of complexity, traditionally, a sequential approach has been preferred, which determines process planning firstly and then performs scheduling independently based on the results. The two sub-problems, however, are complicatedly interrelated to each other, so the IPPS tend to solve the two problems simultaneously. Although many studies for IPPS have been conducted in the past, tool flexibility and capacity constraints are rarely considered. Various meta-heuristics, especially GA, have been applied for IPPS, but the performance is yet satisfactory. To improve solution quality against computation time in GA, we adopted three methods. First, we used a random circular queue during generation of an initial population. It can provide sufficient diversity of individuals at the beginning of GA. Second, we adopted an inferior selection to choose the parents for the crossover and mutation operations. It helps to maintain exploitation capability throughout the evolution process. Third, we employed a modification of the hybrid scheduling algorithm to decode the chromosome of the individual into a schedule, which can generate an active and non-delay schedule. The experimental results show that our proposed algorithm is superior to the current best evolutionary algorithms at most benchmark problems.

Photo-Electrochemical Properties of $TiO_2$ Electrodes Prepared by Anodic Oxidation (양극산화에 의해 제조된 $TiO_2$ 전극의 광전기화학적 성질)

  • Yong Kook Choi;Soon Ki Lee;Q Won Choi;Jeong Sub Seong;Ki Hyung Chjo
    • Journal of the Korean Chemical Society
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    • v.37 no.12
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    • pp.1010-1018
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    • 1993
  • The titanium oxide thin films were prepared by anodic oxidation. The Photo-electrochemical properties of the electrodes were studied in 1 M NaOH solution. The flat band potentials of $TiO_2$ electrodes prepared by anodic oxidation showed around -0.8V and the values were shifted 0.2V to the positive potential direction that of single crystal $TiO_2$. Reduction potential of oxygen by cyclic voltammetry showed around -0.95V vs. SCE and these reactions were processed totally irreversible. The photocurrent of electrodes were showed shorter wavelength than that of single crystal $TiO_2$ and its current density decreased.

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Directional Alignment and Printing of One Dimensional Nanomaterials Using the Combination of Microstructure and Hydrodynamic Force (마이크로 구조 및 동유체력을 이용한 나노와이어 미세 정렬 및 프린팅 기법)

  • Chung, Yongwon;Seo, Jungmok;Lee, Sanggeun;Kwon, Hyukho;Lee, Taeyoon
    • Korean Journal of Materials Research
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    • v.23 no.10
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    • pp.586-591
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    • 2013
  • The printing of nanomaterials onto certain substrates is one of the key technologies behind high-speed interconnection and high-performance electronic devices. For the printing of next-generation electronic devices, a printing process which can be applied to a flexible substrate is needed. A printing process on a flexible substrate requires a lowtemperature, non-vacuum process due to the physical properties of the substrate. In this study, we obtained well-ordered Ag nanowires using modified gravure printing techniques. Ag nanowires are synthesized by a silver nitrate ($AgNO_3$) reduction process in an ethylene glycol solution. Ag nanowires were well aligned by hydrodynamic force on a micro-engraved Si substrate. With the three-dimensional structure of polydimethylsiloxane (PDMS), which has an inverse morphology relative to the micro-engraved Si substrate, the sub-micron alignment of Ag nanowires is possible. This technique can solve the performance problems associated with conventional organic materials. Also, given that this technique enables large-area printing, it has great applicability not only as a next-generation printing technology but also in a range of other fields.

Evidence for a Common Molecular Basis for Sequence Recognition of N3-Guanine and N3-Adenine DNA Adducts Involving the Covalent Bonding Reaction of (+)-CC-1065

  • Park, Hyun-Ju
    • Archives of Pharmacal Research
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    • v.25 no.1
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    • pp.11-24
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    • 2002
  • The antitumor antibiotic (+)-CC-1065 can alkylate N3 of guanine in certain sequences. A previous high-field $^1H$ NMR study on the$(+)-CC-1065d[GCGCAATTG*CGC]_2$ adduct ($^*$ indicates the drug alkylation site) showed that drag modification on N3 of guanine results in protonation of the cross-strand cytosine [Park, H-J.; Hurley, L. H. J. Am. Chem. Soc.1997, 119,629]. In this contribution we describe a further analysis of the NMR data sets together with restrained molecular dynamics. This study provides not only a solution structure of the (+)-CC-1065(N3- guanine) DNA duplex adduct but also new insight into the molecular basis for the sequence- specific interaction between (+)-CC-1065 and N3-guanine in the DNA duplex. On the basis of NOESY data, we propose that the narrow minor groove at the 7T8T step and conformational kinks at the junctions of 16C17A and 18A19T are both related to DNA bending in the drugDNA adduct. Analysis of the one-dimensional $^1H$ NMR (in $H_2O$) data and rMD trajectories strongly suggests that hydrogen bonding linkages between the 8-OH group of the (+)-CC-1065 A-sub-unit and the 9G10C phosphate via a water molecule are present. All the phenomena observed here in the (+)-CC-1065(N3-guanine) adduct at 5'$-AATTG^*$are reminiscent of those obtained from the studies on the (+)-CC-1065(N3-adenine) adduct at $5'-AGTTA^*$, suggesting that (+)-CC-1065 takes advantage of the conformational flexibility of the 5'-TPu step to entrap the bent structure required for the covalent bonding reaction. This study reveals a common molecular basis for (+)-CC-1065 alkylation at both $5'-TTG^*$ and $5'-TTA^*$, which involves a trapping out of sequence-dependent DNA conformational flexibility as well as sequence-dependent general acid and general base catalysis by duplex DNA.

Rigorous Analysis for Optical Impacts of Tapered Sidewall Profile on Trapezoidal Diffraction Grating (사다리꼴 회절격자에서 테이퍼 측면의 광학적 효과에 대한 정확한 분석)

  • Ho, Kwang-Chun
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.20 no.5
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    • pp.151-156
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    • 2020
  • To analyze the diffraction properties of optical signals and the significant impacts of tapered sidewall profile at periodic trapezoidal 2D diffraction gratings, Toeplitz dielectric tensor is first defined and formulated by 2D spatial Fourier expansions associated with trapezoidal profile. The characteristic modes in each layer is then based on eigenvalue problem, and the complete solution is found rigorously in terms of modal transmission-line theory (MTLT) to address the pertinent boundary-value problems. Based on those one, the numerical analysis is performed on how the tapered side profile of grating structures with trapezoidal refractive index distribution affects the design of a sub-wavelength grating reflector. The numerical results reveal that this tapered sidewall profile plays a critical role in determining the reflection bandwidth, the average reflectance, and the band edge.

Asymmetric Joint Scheduling and Rate Control under Reliability Constraints in Cognitive Radio Networks (전파인지 네트워크에서 신뢰성 보장 비대칭 스케줄-데이터율 결합제어)

  • Nguyen, Hung Khanh;Song, Ju-Bin
    • Journal of the Institute of Electronics Engineers of Korea TC
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    • v.49 no.7
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    • pp.23-31
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    • 2012
  • Resource allocation, such as joint rate control and scheduling, is an important issue in cognitive radio networks. However, it is difficult to jointly consider the rate control and scheduling problem due to the stochastic behavior of channel availability in cognitive radio networks. In this paper, we propose an asymmetric joint rate control and scheduling technique under reliability constraints in cognitive radio networks. The joint rate control and scheduling problem is formulated as a convex optimization problem and substantially decomposed into several sub-problems using a dual decomposition method. An algorithm for secondary users to locally update their rate that maximizes the utility of the overall system is also proposed. The results of simulations revealed that the proposed algorithm converges to a globally optimal solution.

Changes of Satsuma Mandarin Sacs during Storage (온주(溫州)밀감 sac의 저장중(貯藏中) 변화(變化))

  • Choi, Young-Bae;Kang, Dong-Sub;Kang, Yeung-Joo
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.16 no.3
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    • pp.21-25
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    • 1987
  • In order to obtain a basic understanding of the storage of Satsuma mandarin sacs, the canned sacs were stored at room temperature from Feb. 20 to June 20(4 months), after dipping in chlorine solution and pasteurization as a pretreatment before canning. In general, the brix, acidity and pH of the sacs were almost not affected by the chlorine treatment ana a variety of pasteurization temperatures during storage. The broken rate was apperently increased by chlorine treatment, especially in the early stage of storage. However, the broken sacs were partially recovered in the end of storage. The contents of total carotenoid were almost not decreased by the conditions of the pretreatments during storage. The contents of vitamin C were sharply decreased between 3rd and 4th month of storage time on all sample. As a result, the decrement of vitamin C in canned sacs was caused by storage temperature rather than the condition of the pretreament.

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Ultra-low-latency services in 5G systems: A perspective from 3GPP standards

  • Jun, Sunmi;Kang, Yoohwa;Kim, Jaeho;Kim, Changki
    • ETRI Journal
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    • v.42 no.5
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    • pp.721-733
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    • 2020
  • Recently, there is an increasing demand for ultra-low-latency (ULL) services such as factory automation, autonomous driving, and telesurgery that must meet an end-to-end latency of less than 10 ms. Fifth-generation (5G) New Radio guarantees 0.5 ms one-way latency, so the feasibility of ULL services is higher than in previous mobile communications. However, this feasibility ensures performance at the radio access network level and requires an innovative 5G network architecture for end-to-end ULL across the entire 5G system. Hence, we survey in detailed two the 3rd Generation Partnership Party (3GPP) standardization activities to ensure low latency at network level. 3GPP standardizes mobile edge computing (MEC), a low-latency solution at the edge network, in Release 15/16 and is standardizing time-sensitive communication in Release 16/17 for interworking 5G systems and IEEE 802.1 time-sensitive networking (TSN), a next-generation industry technology for ensuring low/deterministic latency. We developed a 5G system based on 3GPP Release 15 to support MEC with a potential sub-10 ms end-to-end latency in the edge network. In the near future, to provide ULL services in the external network of a 5G system, we suggest a 5G-IEEE TSN interworking system based on 3GPP Release 16/17 that meets an end-to-end latency of 2 ms.