Journal of Sensor Science and Technology (센서학회지)

The Korean Sensors Society (한국센서학회)
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Fiber-optic fluoroimmunosensor for foodborn pathogens using an optical evanescent field

광섬유 소산장을 이용한 식중독균 신속검출용 형광면역센서

  • Yeom, Se-Hyuk (Department of Sensor and Display Engineering, Kyungpook National University) ;
  • Park, Chang-Sub (Department of Sensor and Display Engineering, Kyungpook National University) ;
  • Kim, Do-Eok (School of Electrical Engineering and Computer Science, Kyungpook National University) ;
  • Kim, Kyu-Jin (School of Electrical Engineering and Computer Science, Kyungpook National University) ;
  • Kang, Byoung-Ho (School of Electrical Engineering and Computer Science, Kyungpook National University) ;
  • Kang, Shin-Won (School of Electrical Engineering and Computer Science, Kyungpook National University)
  • 염세혁 (경북대학교 센서 및 디스플레이공학과) ;
  • 박창섭 (경북대학교 센서 및 디스플레이공학과) ;
  • 김도억 (경북대학교 전자전기컴퓨터학부) ;
  • 김규진 (경북대학교 전자전기컴퓨터학부) ;
  • 강병호 (경북대학교 전자전기컴퓨터학부) ;
  • 강신원 (경북대학교 전자전기컴퓨터학부)
  • Published : 2007.11.30
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Abstract

In this study, the fiber-optic fluoro-immunosensor was designed to detect foodborne pathogens. The fabricated system is composed of the multimode optical fiber on which antibodies are immobilized. Then, a sandwich immunoassay is applied to the fabricated the fiber-optic fluoro-immunosensor. In the "sandwich" binding format, a primary or "capture" antibody is immobilized on the core surface of the multimode optical fiber and a secondary or named as "tracer" antibody is added to the bulk solution. A tracer is labeled FITC (fluorescein isothiocyanate; ${\lambda}ex$=492 nm, ${\lambda}em$= 520 nm). Different concentrations of antigens are tested in different fibers. The detection limit of the fabricated system is 5.08×103 cfu/ml for Vibrio antigen and $0.1{\mu}g/ml$, $0.05{\mu}g/ml$ in non-labeled monolayer phosphate buffered saline (NMP), non-labeled monolayer carbonate bicarbonate buffer (NMC), respectively.

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