• Title/Summary/Keyword: structure elucidation

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Structural Elucidation and Antimicrobial Activity of an Antibiotic, Echinomycin, Produced by Actinomycetes KGT-37 (Actinomycetes KGT-37 균주가 생산하는 항생물질 Echinomycin의 구조해석 및 항균활성)

  • 여운형;김영호;윤봉식;박은경
    • Korean Journal Plant Pathology
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    • v.12 no.3
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    • pp.302-306
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    • 1996
  • 항균물질을 생산하는 방선균 KGT-37를 인삼밭 토양에서 분리하여 배양액으로부터 항균물질을 분리하고 silica gel column chromatography, preparative TLC, HPLC로 정제하였다. \ulcornerH-\ulcornerH COSY, HMQC, HMBC등 NMR 분석결과 KGT-37이 생산하는 항균물질은 cyclic depsipeptide계의 항생물질인 echinomycin으로 동정되었으며 이 연구에서 지금까지 assign되지 않았던 \ulcornerH과\ulcornerC의 NMR signal이 완전히 assign되었다. 이 항균활성물질은 감자더뎅이병균인 Streptomyces scabies과 Corynebacterium lilium을 포함하는 그람 양성 세균에 특히 강한 항균활성을 보였으며, 도열병균 및 점무늬낙엽병균 등 병원곰팡이에도 항균활성을 나타내었다.

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Structure Elucidation of New Cochlioquinol Derivatives from Pathogenic Fungus Bipolaris cynodontis (식물 병원균 Bipolaris cynodontis로부터 분리한 새로운 Cochlioquinol 유도체의 구조 분석)

  • Lim, Chi-Hwan
    • Analytical Science and Technology
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    • v.9 no.1
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    • pp.112-117
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    • 1996
  • Three active compounds were isolated from the culture of a plant pathogenic fungus, Bipolaris cynodontis. The structure elucidation of these compounds was accomplished by 2D NMR techniques, such as $^1H-^1H$ and $^{13}C-^1H$ COSY, COLOC, HMBC and rotating frame NOE(ROESY). Compounds were found as derivatives of cochlioquinone and cochlioquinol that were previously isolated as phytotoxins from B. bicolor and B. cynodontis, respectively. The compounds showed phytotxicity against Italian ryegrass, one of the host plants of B. cynodontis.

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Structure elucidation of a newly isolated saponin from Clerodendrum serratum (L) Moon

  • Bhujbal, Santosh S.;K.Nanda, Rabindra;S.Deoda, Ramesh;Kumar, Dinesh;Kewatkar, Shailesh M.;S.More, Laxmikant;J.Patil, Manohar
    • Advances in Traditional Medicine
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    • v.10 no.4
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    • pp.319-321
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    • 2010
  • Plant saponins are widely distributed amongst plants and have a wide range of biological properties. Icosahydropicenic acid, $C_{51}H_{80}O_{19}$ ((4S,6bS)-8a-((4,5-dihydroxy-6-methyl-3-((3R)-3,4,5-trihydroxy-6-methyl-tetrahydro-2H-pyran-2-yloxy)-tetrahydro-2H-pyran-2-yloxy)carbonyl)-2-hydroxy-4, 6a, 6b, 11, 14b-pentamethyl-11-(2-methylprop-1-enyl)-3-(3,4,5-trihydroxy-6-(hydroxymethyl) - tetrahydro-2Hpyran-2-yloxy)-1, 2, 3, 4, 4a, 5, 6, 6a, 6b, 7, 8, 8a, 9, 10, 11, 12, 12a, 14, 14a, 14b-icosahydropicene-4-carboxylic acid), a new saponin was first time isolated from the roots of Clerodendrum Serratum (L) Moon (Verbenaceae). The structure elucidation of the compound was carried out by $^1H$ NMR and DART-MS studies.

Isolation and Structure Elucidation of a New Glycolipid from the Soft Coral Lobophytum microlobulatum Collected from Havellock Island of Andaman and Nicobar Group of Islands

  • Anjaneyulu, Ammanamanchi S.R.;Rao, Nidasanametla S. Kameswara;Garg, H.S.
    • Natural Product Sciences
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    • v.2 no.2
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    • pp.102-105
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    • 1996
  • A new glycolipid, 2-hydroxy-3 $(octadecyloxy)-propyl-{\alpha}-D-arabinopyranoside$ (1) has been isolated from Lobophytum microlobulatum and its structure has been elucidated by physical and spectral $(UV,\;IR,\;^1H,\;^{13}C\;NMR,\;FABMS)$ data.

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$^{13}C-Nuclear$ Magnetic Resonance of Phenolic Compounds (II) -A Study on the Chemical Shifts of the Phenolic Coumarine Derivatives- (Phenol성(性) 화합물(化合物)의 $^{13}C$-핵자기(核磁氣) 공명(共鳴)(II) -Phenol 성(性) Coumarine 유도체(誘導體)의 Chemical Shift에 관(關)하여-)

  • Ahn, Byung-Zun
    • Korean Journal of Pharmacognosy
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    • v.8 no.1
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    • pp.23-29
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    • 1977
  • The $^{13}C-chemical\;shifts$ of the phenolic coumarine derivatives, aesculetin, daphnetin and herniarin were studied on the basis of my previous report. All spectral data found in this report could be utilized to the structure elucidation of the unknown phenolic coumarine derivatives and other phenolic compounds. In addition, it is suggested that a long range coupling may occur in the following structure as represented by arrow.

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Meroparamycin Production by Newly Isolated Streptomyces sp. Strain MAR01: Taxonomy, Fermentation, Purification and Structural Elucidation

  • El-Naggar Moustafa Y.;El-Assar Samy A.;Abdul-Gawad Sahar M.
    • Journal of Microbiology
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    • v.44 no.4
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    • pp.432-438
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    • 2006
  • Twelve actinomycete strains were isolated from Egyptian soil. The isolated actinomycete strains were then screened with regard to their potential to generate antibiotics. The most potent of the producer strains was selected and identified. The cultural and physiological characteristics of the strain identified. the strain as a member of the genus Streptomyces. The nucleotide sequence of the 16S rRNA gene (1.5kb) of the most potent strain evidenced a 99% similarity with Streptomyces spp. and S. aureofaciens 16S rRNA genes, and the isolated strain was ultimately identified as Streptomyces sp. MAR01. The extraction of the fermentation broth of this strain resulted in the isolation of one major compound, which was active in vitro against gram-positive, gram-negative representatives and Candida albicans. The chemical structure of this bioactive compound was elucidated based on the spectroscopic data obtained from the application of MS, IR, UV, $^1H$ NMR, $^{13}C$ NMR, and elemental analysis techniques. Via comparison to the reference data in the relevant literature and in the database search, this antibiotic, which had a molecular formula of $C_{19}H_{29}NO_2$ and a molecular weight of 303.44, was determined to differ from those produced by this genus as well as the available known antibiotics. Therefore, this antibiotic was designated Meroparamycin.