Meroparamycin Production by Newly Isolated Streptomyces sp. Strain MAR01: Taxonomy, Fermentation, Purification and Structural Elucidation

  • El-Naggar Moustafa Y. (Botany Department, Microbiology Division, Faculty of Science, Alexandria University) ;
  • El-Assar Samy A. (Botany Department, Microbiology Division, Faculty of Science, Alexandria University) ;
  • Abdul-Gawad Sahar M. (Botany Department, Microbiology Division, Faculty of Science, Alexandria University)
  • Published : 2006.08.01

Abstract

Twelve actinomycete strains were isolated from Egyptian soil. The isolated actinomycete strains were then screened with regard to their potential to generate antibiotics. The most potent of the producer strains was selected and identified. The cultural and physiological characteristics of the strain identified. the strain as a member of the genus Streptomyces. The nucleotide sequence of the 16S rRNA gene (1.5kb) of the most potent strain evidenced a 99% similarity with Streptomyces spp. and S. aureofaciens 16S rRNA genes, and the isolated strain was ultimately identified as Streptomyces sp. MAR01. The extraction of the fermentation broth of this strain resulted in the isolation of one major compound, which was active in vitro against gram-positive, gram-negative representatives and Candida albicans. The chemical structure of this bioactive compound was elucidated based on the spectroscopic data obtained from the application of MS, IR, UV, $^1H$ NMR, $^{13}C$ NMR, and elemental analysis techniques. Via comparison to the reference data in the relevant literature and in the database search, this antibiotic, which had a molecular formula of $C_{19}H_{29}NO_2$ and a molecular weight of 303.44, was determined to differ from those produced by this genus as well as the available known antibiotics. Therefore, this antibiotic was designated Meroparamycin.

Keywords

References

  1. Baltz, R.H. 1998. Genetic manipulation of antibiotic producing Streptomyces. Trends in Microbiol. 6, 76-83 https://doi.org/10.1016/S0966-842X(97)01161-X
  2. Ben-Fguira, L.F., S. Fosto, R.B. Mehdi, L. Mellouli, and H. Laatsch. 2005. Purification and structure elucidation of antifungal and antibacterial activities of newly isolated Streptomyces sp. strain US80. Microbiol. Res. 156, 341-347 https://doi.org/10.1016/j.resmic.2004.10.006
  3. Bhavanani, S.M. and C.H. Ballow. 2000. New agents for gram-positive bacteria. Curr. Op. Microbiol. 3, 528-534 https://doi.org/10.1016/S1369-5274(00)00134-X
  4. Edwards, U., T. Rogall, H. Bocker, M. Emade, and E. Bottger. 1989. Isolation and direct complete nucleotide determination of entire genes. Characterization of a gene coding for 16S ribosomal DNA. Nucleic Acid Res. 17, 7843-7853 https://doi.org/10.1093/nar/17.19.7843
  5. El-Naggar, M.Y., M.A. Hassan, W.Y. Said, and S.A. El-Aassar. 2003. Effect of support materials on antibiotic MSW2000 production by immobilized Streptomyces violatus. J. Gen. Appl. Microbiol. 49, 235-243 https://doi.org/10.2323/jgam.49.235
  6. Furumai, T., Y. Igarashi, H. Higuchi, N. Saito, and T. Oki. 2002. Kosinostatin, a quinocycline antibiotic with antitumor activity from Micromonospora sp. TP-A0468. J. Antibiot. 55, 128-133 https://doi.org/10.7164/antibiotics.55.128
  7. Hall, T.A. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl. Acids Symp. Ser. 41, 95-98
  8. Kornerup, A. and J.H. Wanscher. 1978. Methuen Handbook of colour (ed.). Methuen, London, UK
  9. Laatsch, H. 2003. A natural products database for rapid structure determination, chemical concepts. Weinheim, Germany
  10. Lechevalier, H.A. 1975. Production of the same antibiotics by members of different genera of microorganisms. Adv. Appl. Microbiol. 19, 25-45 https://doi.org/10.1016/S0065-2164(08)70421-0
  11. Lechevalier, H.A., S.T. Willium, M.E. Sharpe, and J.G. Holt. 1989. The Actinomycetes: A practical guide to genetic identification of actinomycetes. p. 2344-3330. In, Bergy's Manual of Determinative Bacteriology. The Williams & Wilkins Co., Baltimore, Maryland, USA
  12. Miyadoh, S. 1993. Research on antibiotic screening in Japan over the last decade: a producing microorganisms approach. Actinomycetologica. 9, 100-106
  13. Motta, A.S., F. Cladera-Olivera, and A. Brandelli. 2004. Screening for antimicrobial activity among bacteria isolated from the Amazon Basin. Brazilian J. Microbiol. 35, 307-310 https://doi.org/10.1590/S1517-83822004000300007
  14. Okami, Y. and K. Hotta. 1988. Search and discovery of new antibiotics. p. 33-67. In M. Goodfellow, S.T. Williams, and M. Mordarski (eds.), Actinomycetes in Biotechnology. Academic Press, London, UK
  15. Okazaki, T. and Y. Okami. 1972. Studies on marine microorganisms. II. Actinomycetes in Sagami Bay and their antibiotic substances. J. antibiot. 25, 461-466 https://doi.org/10.7164/antibiotics.25.461
  16. Page, R.D.M. 1996. TREEVIEW: An application to display phylogenetic trees on personal computers. Computer Appl. Biosci. 12, 357-358
  17. Pamboukian, C.R.D. and M.C.R. Facciotti. 2004. Production of the antitumoral retamycin during continuous fermentations of Streptomyces olindensis. Process Biochem. 39, 2249- 2255 https://doi.org/10.1016/j.procbio.2003.11.006
  18. Saadoun, I. and R. Gharaibeh. 2003. The Streptomyces flora of Badia region of Jordan and its potential as a source of antibiotics active against antibiotic-resistant bacteria. J. Arid. Environ. 53, 365-371 https://doi.org/10.1006/jare.2002.1043
  19. Sambrook, J., E.F. Fritsch, and T. Maniatis. 1989. Molecular cloning. A laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, USA
  20. Sanger, F., S. Nicklen, and A.R. Coulson. 1977. DNA sequencing with chain terminator inhibitors. Proc. Natl. Acad. Sci. 74, 5463-5467
  21. Shirling, E.B. and D. Gottlieb. 1966. Methods for characterization of Streptomyces species. Int. J. Syst. Bacteriol. 16, 313-340 https://doi.org/10.1099/00207713-16-3-313
  22. Stryzhkova, H.M., O.P. Kopeiko, V.L. Lavrinchuk, O.L. Bambura, and B.P. Matseliukh. 2002. Spontaneous and induced variability of Streptomyces aureofaciens chlortetracycline producer. Mikrobiol. Zentable. 64, 19-23
  23. Tanaka, Y. T. and S.O. Mura. 1993. Agroactive compounds of microbial origin. Ann. Rev. Microbiol. 47, 57-87 https://doi.org/10.1146/annurev.mi.47.100193.000421
  24. White, J.D., R. Hanselmann, R.W. Jackson, W.J. Porter, Y. Ohba, T. Tiller, and S. Wang. 2001. Total synthesis of rutamycin B, a macrolide antibiotic from Streptomyces aureofacins. J. Org. Chem. 66, 5217-5231 https://doi.org/10.1021/jo0104429
  25. Yang, S.S. and M.Y. Ling. 1989. Tetracycline production with sweet potato residue by solid-state fermentation. J. Biotechnol. Bioeng. 33, 1021-1028 https://doi.org/10.1002/bit.260330811