• Korean Journal of Veterinary Research
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• v.36 no.3
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• pp.657-663
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• 1996

The API STAPH system was compared with conventional methods for identification of 214 strains of Staphylococcus hyicus subsp. hyicus isolated from cases of exudative epidermitis in piglets, skin of healthy pigs, skin of healthy chickens and bovine intramammary infections, and biochemical characteristics among the swine, avian and bovine strains were also compared. All of the swine and bovine strains produced acid within 24 hours from fructose, lactose and trehalose by conventional methods, but some of the avian strains showed a delayed positive reaction in these carbohydrates. These delayed positive strains in conventional methods gave usually negative results for them in the API STAPH system. With the API STAPH system, eighteen different profile numbers were encountered in 214 strains of swine, avian and bovine origin. The swine and bovine strains, respectively, were distributed among 4 profiles, while the avian strains were distributed among 17 profiles. The profile number observed most frequently in the strains of each animal species was uniformly 6 516 153. By conventional methods, approximately 96% of the swine strains were positive for ${\beta}$-glucuronidase, but not in any strains from chickens and cattle. For hyaluronidase production determined by degradation of sodium hyaluronidate in a solid culture medium, all the swine and bovine strains were positive, but only 37.5% of the avian strains were positive for it. From these findings, there were differences in the production of extracellular active substances between swine strains of Staphylococcus hyicus subsp. hyicus and those isolated from chickens and cattle.

• The Korean Journal of Food And Nutrition
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• v.7 no.3
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• pp.203-212
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• 1994

The nangmyun is a Korean iced noodle made by putting buckwheat-noodles into cold broth. Seven samples of nangmyun-broths were collected from Korean restaurants in Bucheon during July 1994. The contamination levels of nangmyun-broths by coliform bacteria were determined, and then the 40 colonies of coliform bacteria, isolated randomly from 4 samples of nangmyun-broths, were identified at genus or species level with the additional test for psychrotrophic character The coliform counts in nangmyun-broths were 6.0x102-6.5$\times$104/ml(average 2.3$\times$104%). Among the 40 strains of isolates, 27 strains(67.5%) were identified as the genus Klebsiella, 9 strains(22.5%) as the genus Enterobacter, 2 strains(5.0%) as the genus Citrobacter and 2 strains (5.0%) as the genus Escherichia. Among 27 strains of Klebsiella, 11 strains (40.8%) were identified as K planticola, 4 strains(14.8%) as K. pneumoniae, 2 strains(7.4%) as K. ozaenae and 2 strains(7.4%) as K. terrigena, but 8 strains(29.6%) of a typic\ulcorner1 Klebgiella could not be Identified at species level. All the 40 strains of coliform bacteria were psychrotrophs showing slow growth at 1$0^{\circ}C$, and 18 strains(45%) grew at 5$^{\circ}C$. It was thought to be a good basic data In describing the reason for too high coliform counts in nangmyun-broths that all coliform bacteria tested In this study were psychrotrophs.

• Journal of Microbiology and Biotechnology
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• v.27 no.5
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• pp.909-915
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• 2017

Bifidobacterium bifidum BGN4 (BGN4) has many proven beneficial effects, including antiallergy and anticancer properties. It has been commercialized and used in several probiotic products, and thus strain-specific identification of this strain is very valuable for further strain-dependent physiological study. For this purpose, we developed novel multiplex polymerase chain reaction (PCR) primer sets for strain-specific detection of BGN4 in commercial products and fecal samples of animal models. The primer set was tested on seven strains of B. bifidum and 75 strains of the other Bifidobacterium species. The BGN4-specific regions were derived using megaBLAST against genome sequences of various B. bifidum databases and four sets of primers were designed. As a result, only BGN4 produced four PCR products simultaneously whereas the other strains did not. The PCR detection limit using BGN4-specific primer sets was $2.8{\times}10^1CFU/ml$ of BGN4. Those primer sets also detected and identified BGN4 in the probiotic products containing BNG4 and fecal samples from a BGN4-fed animal model with high specificity. Our results indicate that the PCR assay from this study is an efficient tool for the simple, rapid, and reliable identification of BGN4, for which probiotic strains are known.

• Journal of Aquaculture
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• v.3 no.1
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• pp.31-37
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• 1990

Ten strains of tilapiine species from genus Oreochromis were cytogenetically studied for genetic stock identification. Both the chromosome numbers(2n=44) were identical in all 10 strains. Heteromorphic sex chromosome pair were not found in any strains. Nuclear volumes vary between O. niloticus(21.0 $\mu$ $m^3$) and O. aureus(22.4 $\mu$ $m^3$)

• The Journal of the Korean Society for Microbiology
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• v.21 no.4
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• pp.417-422
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• 1986

The specimens were collected from 89 diarrheal patients who had visited Pusan National University Hospital from June to September 1985. They were cultured and tested for the bacteriological identification of causative agents. In this study we identified 5 strains of Salmonella species, 5 strains of Shigella species, 2 strains of Y. enterocolitica, and 17 strains of enteric pathogenic E. coli. Enteric pathogenic E. coli were classified into enterotoxigenic E. coli, enteropathogenic E. coli, and enteroinvasive E. coli by serological type. We tried to isolate V. cholerae and V. parahaemolyticus too but we cannot find them out.

• International Journal of Oral Biology
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• v.31 no.1
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• pp.11-14
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• 2006

This study was undertaken to develop PCR primers for the identification and detection of Streptococcus anginosus using species-specific forward and reverse primers. These primers targeted the variable regions of the 16S ribosomal RNA coding gene(rDNA). The primer specificity was tested against 12 S. anginosus strains and 6 different species(10 strains) of oral bacteria. The primer sensitivity was determined by testing serial dilutions of the purified genomic DNA of S. anginosus ATCC $33397^T$. The data showed that species-specific amplicons were obtained from all the S. anginosus strains tested, but not in the six other species. The PCR could detect as little as 0.4pg of the chromosomal DNA from S. anginosus. This suggests that the PCR primers are highly sensitive and applicable to the detection and identification of S. anginosus.

• Mycobiology
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• v.50 no.4
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• pp.231-237
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• 2022

Penicillium species have been actively studied in various fields, and many new and unrecorded species continue to be reported in Korea. Moreover, unidentified and misidentified Korean Penicillium species still exist in GenBank. Therefore, it is necessary to revise the Korean Penicillium inventory based on accurate identification. We collected Korean Penicillium nucleotide sequence records from GenBank using the newly developed software, GenMine, and re-identified Korean Penicillium based on the maximum likelihood trees. A total of 1681 Korean Penicillium GenBank nucleotide sequence records were collected from GenBank. In these records, 1208 strains with four major genes (Internal Transcribed Spacer rDNA region, b-tubulin, Calmodulin and RNA polymerase II) were selected for Penicillium reidentification. Among 1208 strains, 927 were identified, 82 were identified as other genera, the rest remained undetermined due to low phylogenetic resolution. Identified strains consisted of 206 Penicillium species, including 156 recorded species and 50 new species candidates. However, 37 species recorded in the national list of species in Korea were not found in GenBank. Further studies on the presence or absence of these species are required through literature investigation, additional sampling, and sequencing. Our study can be the basis for updating the Korean Penicillium inventory.

• Journal of Mushroom
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• v.7 no.2
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• pp.49-56
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• 2009

This study was conducted to preserve of mushroom resources and utility useful wild mushrooms by segregation and identification from 2005 to 2007. The mushroom strains were collected a center of native mushroom wild growth place of Chungbuk Province. The obtained results from this study were summarized as follows ; We collected 79 wild mushroom strains, and the collected wild mushrooms were composition of 32 strains of edible mushrooms, 3 strains of medicinal use mushrooms, 15 strains of poisonous mushrooms, and 29 strains indistinct mushrooms. The 28 strains were segregated and identified from 32 strains of edible mushrooms. The present preservation strains are 15 strains, and other 13 strains were damaged in tissue culture and preservation. We made specimen of wild mushroom by alcohol, and have preserved perennial mushrooms by drying. We photographed 79 strains of wild mushrooms.

• The Journal of the Korean Society for Microbiology
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• v.7 no.1
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• pp.43-49
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• 1972

Mycotic infection seems to be increasing in importance as the causal agents of disease in man, especially invaders in already debilitated persons. This paper presents the identification of 39 stock strains of yeast like cells which were isolated from patients at National Medical Center. It reveals 21 strains of C. albicans, 5 strains of T. glabrata, 4 strains of C. tropicalis, one strain of T. mogii & 8 strains of unidentified.

• Microbiology and Biotechnology Letters
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• v.22 no.5
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• pp.544-549
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• 1994

Lactobacilli proliferating in fermented sausages of the specific ripening conditions were isolated from fermented sausages, manufactured in the absence of an added starter, during ripening under controlled temperature-humidity conditions. Based on morphological, physiological and bio- chemical characteristics and carbohydrate fermentation of isolated strains, three strains of isolates were identified as Lactobacillus curvatus, two strains as Lactobacillus sake. Optimal temperature and pH for growth of isolated strains were 30$\circ$C and pH 6.0~7.0, respectively. These strains were salt tolerant, multiplying in the presense of 6~8% NaCl.