• Journal of Microbiology and Biotechnology
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• v.18 no.10
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• pp.1630-1633
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• 2008

A novel putative toxin-antitoxin segregational stability system named KyAB system was identified in a novel native plasmid pBMB8240 from Bacillus thuringiensis strain YBT-1520, based on sequences homology with other toxin-antitoxin systems, the lethal activity of the KyB putative toxin in Escherichia coli and the stabilizing effect of the kyAB system in Bacillus thuringiensis. Secondarily, the native plasmid pBMB9741 from the same strain was resequenced and the corrected plasmid was named as pBMB7635. Based on sequence homology with the tasAB system and the lethal activity of toxin protein in Escherichia coli, a tasAB-like putative toxin-antitoxin system was identified on pBMB7635.

• Proceedings of the KSME Conference
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• 2003.11a
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• pp.1086-1091
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• 2003

The problem of residual stresses and fatigue behavior in welded structures is the main concern of welding research fields. The residual stresses and distortions of structures by welding exert negative effect on the safety of mechanical structures. Postweld heat treatment is usually carried out to relieve this residual stresses of welded joints. In this paper the influence of postweld heat treatment on fatigue life of butt-welded joint was investigated. To predict the effect of PWHT, an analytical model is developed by finite element and local strain approach and the result of fatigue life analysis is compared to experimental results. It is demonstrated that fatigue life estimates closely approximate the experimental results and PWHT provides some increase of fatigue lives in long-life fatigue region and no increase in short-life fatigue region because of the residual stress relaxation under tensile loads.

• The Korean Journal of Mycology
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• v.51 no.2
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• pp.101-109
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• 2023

A fungal strain designated KNUF-21-66Q1 was isolated from soil in Chungcheongbuk Province, Korea. Moderate growth of colonies was observed on potato dextrose agar, oatmeal agar (OA), malt extract agar, and cornmeal agar media at 25℃, and the detailed morphology was examined on OA medium. The colonies on OA medium were flat, had entire margin, hyaline, and yellow concentric rings in 3-4 weeks. Conidiomata were pycnidial, solitary or clustered, globose to subglobose, black-brown, and 300-500 ㎛ in diameter. Conidiogenous cells were smooth, hyaline, globose to ampulliform, and 6.0-9.0×3.0-6.0 ㎛ in size (n=15). Conidia were hyaline to pale brown, slightly golden, obovoid to slightly ellipsoidal, smooth, guttulate, and 3.0-4.7×2.1-3.3 ㎛ in size (n=100). The strain was confirmed based on phylogenetic analysis using internal transcribed spacer regions, the partial 28S rDNA of large subunit, and β-tubulin gene sequences. The morphological observations and phylogenetic analysis revealed that the strain KNUF-21-66Q1 was similar to the previously described Paraconiothyrium kelleni. To our knowledge, this is the first report of P. kelleni in Korea.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.25 no.10
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• pp.1528-1534
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• 2001

In order to improve Leak-Be(ore-Break methodology, more precisely the crack growth evaluation, a round robin analysis was proposed by the CEA Saclay. The aim of this analysis was to evaluate the crack initiation life, penetration life and shape of through wall crack under cyclic bending loads. The proposed round robin analysis is composed of three main topic; fatigue crack initiation, crack propagation and crack penetration. This paper deals with the first topic, crack initiation in a notched pipe under four point bending. Both elastic-plastic finite element analysis and Neuber's rule were used to estimate the crack initiation life and the finite element models were verified by mesh-refinement, stress distribution and global deflection. In elastic-plastic finite element analysis, crack initiation life was determined by strain amplitude at the notch tip and strain-life curve of the material. In the analytical method, Neuber's rule with the consideration of load history and mean stress effect, was used for the life estimation. The effect of notch tip radius, strain range, cyclic hardening rule were examined in this study. When these results were compared with the experimental ones, the global deformation was a good agreement but the crack initiation cycle was higher than the experimental result.

• Korean Journal of Environmental Agriculture
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• v.24 no.4
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• pp.379-385
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• 2005

This study was conducted to find out a useful bioremediation technology for heavy metal contaminated soil and water. We isolated strain CPB from heavy metal contaminated soil and evaluated the tolerance level and adsorption capacity of strain CPB to heavy metals (Strain is not determined yet). Strain CPB showed variable tolerance limit to different kinds heavy metal or concentrations of heavy metals. The growth of strain CPB was significantly inhibited by mixed heavy metals (Cd+Cu+Pb+Zn) than that of by single heavy metal. Strain CPB showed high binding capacity with Pb (Pb>Cd>Cu>Zn). In general, strain CPB showed high uptake of heavy metals such as Pb, Cd and Cu. It was observed that the capacity of heavy metal uptake from mixture of heavy metals was reduced in comparison with single heavy metal treatment. But total contents of heavy metal bound with cell in mixed heavy metal showed higher than in single heavy metal treatment. Heavy metal adsorption in cells was affected by several external factors, such as temperature and pH etc.. The optimum temperature and pH of the adsorption of heavy metal into cells were ca. $25{\sim}35^{\circ}C$ and pH ca. $5{\sim}7$, respectively. A large number of the electron dense particles were found mainly on the cell wall and cell membrane fractions, which was determined by transmission electron microscope. Energy dispersive X-ray spectroscopy revealed that the electron dense particles were the heavy metal complexes the substances binding with heavy metals.

• Korean Journal of Microbiology
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• v.54 no.3
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• pp.289-292
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• 2018

Using pyrene as the enrichment nutrient, a bacterial strain 10PY1A, was isolated by enrichment culture from oil-contaminated sea sand of Arabian Gulf in Saudi Arabia, and this strain belongs to the species Idiomarina piscisalsi, based on 16S RNA gene sequence analysis. The genome of I. piscisalsi strain 10PY1A contains 2,346 protein-coding sequences and an average GC content of 47.4% in its chromosome (2.59 Mbp). Genes encoding proteins related to the degradation of pyrene were existed in the strain 10PY1A genome, indicating that this strain can be used to degrade polycyclic aromatic hydrocarbons in oil-contaminated marine flora and soil.

• Transactions of the Korean Society of Mechanical Engineers
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• v.19 no.5
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• pp.1182-1189
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• 1995

Several methods have been developed to predict the creep rupture time of the steam pipes in thermal power plant. However, existing creep life prediction methods give very conservative value at operating stress of power plant and creep rupture strain cannot be well estimated. Therefore, in this study, creep rupture time and strain prediction method accounting for material damage and grain boundary sliding is newly proposed and compared with the existing experimental data. The creep damage evolves by continuous cavity nucleation and constrained cavity growth. The results showed good correlation between the theoretically predicted creep rupture time and the experimental data. And creep rupture strain may be well estimated by using the proposed method.

• Journal of the Korean Society for Railway
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• v.7 no.2
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• pp.85-92
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• 2004

This paper consists of two parts. One is finite element analysis of the redistribution of residual stresses of weld specimen by cutting. This work is necessary to predict the actual residual stress distribution of weld specimens used in fatigue test. The other subject is to calculate the relaxation of residual stress and the strain field induced by cyclic loading. To obtain fatigue life of weldment, the value of strain amplitude at each position is necessary, for example in the strain-life approach, and the numerical results can be used to verify experimental strain measurements. Thermo mechanical finite element analyses were conducted on the commercial package ABAQUS.

• Journal of Applied Biological Chemistry
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• v.43 no.2
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• pp.74-78
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• 2000

A strain that produces a high level of carboxymethyl cellulase was isolated from rotten leaves. The isolated strain was revealed to be gram-negative, oxidase-positive, and catalase-negative. From the electron microscopic features, it was identified as a rod-shaped bacterium with peritrichous flagella and did not form spores. Morphological and biochemical characteristics of the strain were found to be similar to the Pseudomonas species. However, carbon utilization test showed different results. Based on the results, this new strain was identified as Pseudomonas sp. CMC-50. CMCase produced by this strain showed a strong activity in neutral and weak acidic conditions and maximum activity at $50^{\circ}C$.

• Journal of Life Science
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• v.11 no.3
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• pp.273-278
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• 2001

Removal of nitrogen compound from waste water is essential and often accomplished by biological process. Deni-trification bacterium. Paracoccus denitrificans(KCTC 2350) is employed to estimate the ability and the characteristics of denitrification. In the immobilized biological reactor system, the measurement of absolute amount of active strain in the reactor is comparatively difficult or impossible. In this study, strain immobilized denitrification reactor was designed with the unwoven texture wrapped peeped hole plastic tube to calculated the absolute amount of active strain by comparing the activity of the immobilized reactor adn the free cell reactor. The reactor system was continuous stirred tank reactor and the rate of substrate consumption was assumed to be Michaelis-Menten equation. As a result, we found that the amount of immobilized active strain was the half of the total active strain in the reactor and the time required to reach in the equilibrium state in the immobilized reactor system was shorter than that of the free cell reactor system.