• Journal of Microbiology and Biotechnology
• /
• 제24권5호
• /
• pp.667-674
• /
• 2014

Twenty-five Saccharomyces cerevisiae strains were screened for the highest sugar tolerance, ethanol-tolerance, ethanol production, and inhibitor resistance, and S. cerevisiae KL5 was selected as the best strain. Inhibitor cocktail (100%) was composed of 75 mM formic acid, 75 mM acetic acid, 30 mM furfural, 30 mM hydroxymethyl furfural (HMF), and 2.7 mM vanillin. The cells of strain KL5 were treated with ${\gamma}$-irradiation, and among the survivals, KL5-G2 with improved inhibitor resistance and the highest ethanol yield in the presence of inhibitor cocktail was selected. The KL5-G2 strain was adapted to inhibitor cocktail by sequential transfer of cultures to a minimal YNB medium containing increasing concentrations of inhibitor cocktail. After 10 times of adaptation, most of the isolated colonies could grow in YNB with 80% inhibitor cocktail, whereas the parental KL5 strain could not grow at all. Among the various adapted strains, the best strain (KL5-G2-A9) producing the highest ethanol yield in the presence of inhibitor cocktail was selected. In a complex YP medium containing 60% inhibitor cocktail and 5% glucose, the theoretical yield and productivity (at 48 h) of KL5-G2-A9 were 81.3% and 0.304 g/l/h, respectively, whereas those of KL5 were 20.8% and 0.072 g/l/h, respectively. KL5-G2-A9 reduced the concentrations of HMF, furfural, and vanillin in the medium in much faster rates than KL5.

• Journal of Microbiology and Biotechnology
• /
• 제11권2호
• /
• pp.222-228
• /
• 2001

Vibrio metschnikovii strain RH530 is a non-pathogenic, industrially-important alkaline protease producer which has been isolated from wastewater. In this paper, we report on the transformation of this strain by using the method of electroporation. A field strength of $7.5\;kVcm^{-1}$ and $25\;{\mu}F$, and using a 0.2-cm cuvette, appeared to be the optimal conditions for electroporation of the cells with the recombinant pSBCm plasmid carrying the vapK alkaline protease gene and the ColE1 replicon. Cells were subjected to osmotic shock in order to remove extracelluar DNase, and adding 200 mM of sucrose to electroporation buffer cells showed an increased transformation efficiency. Maximum efficiency of transformation was obtained at an early exponential growth phase. Using all of the conditions mentioned above, we routinely obtained a transformation efficiency of more than $10^4{({\mu}g\;plasmid\;DNA)}^{-1}$. The stability of the plasmid pSBCm in V. metschnikovii RH530 was 25% after 18h of growth (27 generations) in the medium without antibiotic selection. The insertion of the par locus to the pSBCm increased the stability of the plasmid up to 42% without selective pressure. The increase in plasmid stability was accompanied by the increase in the productivity of alkaline protease in the recombinant V. metschnikovii strain RH530. Determining optimal conditions for the transformation of the industrially-important, nonpathogenic Vibrio strain, and the improvement of plasmid stability by introducing the par locus into the high copy number plasmid vector, will allow the development of procedures involved in the genetic manipulation of this strain, particularly for its use in the production of industrial enzymes such as alkaline protease.

• 한국미생물·생명공학회지
• /
• 제27권2호
• /
• pp.145-152
• /
• 1999

Brettanomyces custersii CBS 5512 which has reported as a thermotolerant glucose-cellobiose co-fermentable yeast strain was mutated with UV and NTG to improve ethanol yield at higher than 4$0^{\circ}C$ B. custersii H1-23, H1-39, H1-55 and H1062 were finally selected for hyper-fermentable strains at higher than 4$0^{\circ}C$ from thermotolerant 7510 colonies through 5th selection. Among the selected strains, H1-39 mutant had better fermentability at 4$0^{\circ}C$ and 43$^{\circ}C$ from different concentrations of glucose. H1-39 and H1-23 mutants yielded more than 70% of the theoretical ethanol yield in 4 and 8% mixed sugars at above 4$0^{\circ}C$, which was 5-11% higher than those by original strain. Especially, H1-39 mutant had better fermentability in 4% mixed sugar. It showed 78.5% of the theoretical yield at 4$0^{\circ}C$ and 72.2% of the theoretical yield at 43$^{\circ}C$. On the other hand, theoretical yield of ethanol by H1-39 mutant in 8% mixed sugar at 4$0^{\circ}C$ and 43$^{\circ}C$ were 75.2% and 70.2%, respectively. Theses values increased up to 7-11% as compared to those by orginal strain. By the simultaneous saccharification and fermentation, ethanol production by H1-39 mutant increased up to more than 23% as compared to that by original strain.

• Smart Structures and Systems
• /
• 제24권6호
• /
• pp.709-721
• /
• 2019

This paper investigates the framework of vision-based dense displacement and strain measurement of miter gates with the approach for the quantitative evaluation of the expected performance. The proposed framework consists of the following steps: (i) Estimation of 3D displacement and strain from images before and after deformation (water-fill event), (ii) evaluation of the expected performance of the measurement, and (iii) selection of measurement setting with the highest expected accuracy. The framework first estimates the full-field optical flow between the images before and after water-fill event, and project the flow to the finite element (FE) model to estimate the 3D displacement and strain. Then, the expected displacement/strain estimation accuracy is evaluated at each node/element of the FE model. Finally, methods and measurement settings with the highest expected accuracy are selected to achieve the best results from the field measurement. A physics-based graphics model (PBGM) of miter gates of the Greenup Lock and Dam with the updated texturing step is used to simulate the vision-based measurements in a photo-realistic environment and evaluate the expected performance of different measurement plans (camera properties, camera placement, post-processing algorithms). The framework investigated in this paper can be used to analyze and optimize the performance of the measurement with different camera placement and post-processing steps prior to the field test.

• 적정기술학회지
• /
• 제7권1호
• /
• pp.2-25
• /
• 2021

본 연구에서는 일반적으로 협동 로봇의 스킨으로 사용될 수 있는 고분자 재료 선정 및 기계적 특성 검사를 진행하고, 각 재료에 대한 성별 선호도 설문조사를 진행하였다. 조사는 20~30세의 근무자 225명(남: 124명, 여: 101명)을 대상으로 작업 중 로봇과 가장 많이 접촉하는 어깨, 팔꿈치 별로 선정된 Dragon-skin, Ecoflex, 및 polydimethylsiloxane(PDMS)에 대한 성별에 따른 선호도 조사로 진행하였다. 설문은 각각 설문자들이 느끼는 재료에 대한 인식 단단함, 끈적임, 익숙함, 선호도 4종류로 구분하여 진행되었고, 단단함과 끈적임은 각각 재료의 변형률과 접촉각으로 측정되었다. 선호도 조사 결과, 여성은 변형률이 작은, 더 단단한 재료를 선호하는 반면, 남성은 변형률이 큰 부드러운 재료를 선호했다. 성별에 따른 선호도와 관련하여 재료의 특성을 평가한 결과, 여성은 끈적임이 낮고 변형률이 낮은 Dragon-skin을 선호하는 경향이 있는 반면, 남성은 끈적임에 관계없이 변형률이 높은 Ecoflex를 선호하는 경향이 있음을 확인하였다. 따라서 이러한 결과는 협동 로봇 스킨 제작을 고려할 때 재료 선택에 기준이 될 것으로 보인다.

• Journal of Microbiology and Biotechnology
• /
• 제16권8호
• /
• pp.1269-1275
• /
• 2006

The selection of multienzyme complex-producing bacteria under aerobic condition was conducted for improving the degradation of lignocellulosic substances. The criteria for selection were cellulase and xylanase enzyme production, the presence of cellulose-binding domains and/or xylan-binding domains in enzymes to bind to insoluble substances, the adhesion of bacterial cells to insoluble substances, and the production of multiple cellulases and xylanases in a form of a high molecular weight complex. Among the six Bacillus strains, isolated from various sources and deposited in our laboratory, Paenibacillus curdlanolyticus B-6 strain was the best producer of cellulase and xylanase enzymes, which have both cellulose-binding factors (CBFs) and xylan-binding factors (XBFs). Moreover, multiple carboxymethyl cellulases (CMCases) and xylanases were produced by the strain B-6. The zymograms analysis showed at least 9 types of xylanases and 6 types of CMCases associated in a protein band of xylanase and cellulase with high molecular weight. These cells also enabled to adhere to both avicel and insoluble xylan, which were analyzed by scanning electron microscopy. The results indicated that the strain B-6 produced the multienzyme complex, which may be cellulosome or xylanosome. Thus, P. curdlanolyticus B-6 was selected to study the role and interaction between the enzymes and their substrates and the cooperation of multiple enzymes to enhance the hydrolysis due to the complex structure for efficient cellulases and xylanases degradation of insoluble polysaccharides.

• 한국미생물·생명공학회지
• /
• 제39권2호
• /
• pp.111-118
• /
• 2011

교배와 원형질체 융합을 통한 배수체인 야생형 산업균주의 개발을 위하여, hybrid의 선별표지로서 우성의 선별표지인 aureobasidin A 내성이 사용될 수 있는 지를 알아보고자 하였다. 선별배지에서 aureobasidin A의 최적농도는 야생형 균주인 경우 SD와 YPD 배지에서는 0.5 ${\mu}g$/mL 이상이었고, SG와 YPG에서는 0.2-0.3 ${\mu}g$/mL 이었다. 한편 호흡결여돌연변이주는 야생형 균주보다 훨씬 높은 농도의 aureobasidin A에도 내성이 있음을 나타내었다. 우리는 K114/YIP균주의 전분분해 능력이 배수체 야생형 산업 균주에 전달 될 수 있는지를 이 방법을 통하여 관찰하였다. 반수체 영양요구성 균주 K114/YIP에 aureobasidin에 대한 내성을 부여하는 pAUR112가 도입된 균주와 야생형 균주 KL 혹은 C6와의 rare-mating 후 aureobasidin A 함유 배지에서 성장한 hybrid를 분리할 수 있었다. Hybrid는 전분분해 능력을 함유하고 있었을 뿐 아니라 두 양친의 특성을 동시에 지녔으며, 전자현미경 관찰 결과에서도 hybrid는 양친주의 특성을 모두 갖는 것으로 나타났다.

• 한국미생물·생명공학회지
• /
• 제26권6호
• /
• pp.562-565
• /
• 1998

Nisin-producing and nisin resistant L. lactis subsp. lactis ATCC7962 harbored six plasmids. To find a plasmid containing a nisin resistant gene, these plasmids were transformed into L lactis LM0230 of plasmid-free and nisin sensitive strain. After screening on nisin selection media containing nisin (150 $\mu\textrm{g}$/$m\ell$), several nisin resistant transformants were obtained and the level of nisin resistance was very similar to that of wild type L lactis subsp. lactis ATCC7962. A 26.5 kb plasmid, named as pCS100, which confers resistance to nisin, was identified in transformants. The pCS100 was digested with EcoRI and Southern blot hybridization was done with nisI probe to localize the nisin resistant gene. A 4 kb EcoRI fragment showed a strong positive signal, and it was cloned into pBluescript for the potential selection marker.

• Journal of Microbiology and Biotechnology
• /
• 제7권5호
• /
• pp.333-340
• /
• 1997

An actinomycetes, KT-10 isolated from ginseng field in Kyongpook, Korea was selected based on its ability to produce a lysosomal cathepsin B inhibitor. The inhibitor purified from the culture supernatant of the isolate KT-10 showed strong inhibitory effects against cathepsin B as well as against papain when the activities were measured using synthetic substrate, ${\alpha}$-N-benzyloxycarbonyl-L-Iysine p-nitrophenyl ester (CLN) or ${\alpha}$-N-benzoyl-D,L-arginine 2-naphthylamide (BANA). The isolate KT-10 was identified as a species of Streptomyces based on its morphological characteristics and chemotaxonomic data. The TAXON program of Ward was used to identify Streptomyces sp. KT-10 as a strain of Streptomyces luteogriseus belong to cluster 18 of the genus Streptomyces with a Willcox probability 0.999388. The cathepsin B inhibitor was presumed to a novel material composed of a polyhydroxylamine.

• 한국정밀공학회:학술대회논문집
• /
• 한국정밀공학회 2002년도 춘계학술대회 논문집
• /
• pp.373-377
• /
• 2002

In the end milling operation the deflection of the cutter is an important factor affecting the accuracy of machining with implications on the selection of cutting parameters and economics of the operation. The deflection of the end mill was studied both experimentally with strain gauge, tool dynamometer, laser measuring apparatus and on a finite element model of the cutting using ANSYS software. The deflection of machining tool with various helix angles was studied with FEM simulation and experiment. ANSYS analysis performed on the finite element model of the end mill provides deflection results which agree within 15.0% with the experimental ones.