• Journal of Microbiology and Biotechnology
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• v.4 no.4
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• pp.338-342
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• 1994

The bacterium producing EPA was isolated from the intestines of the marine fishes. The strains were studied for their identification and their culture conditions. The selected strain was gram negative, rod(0.7 $\times$ 2.4 $\mu m$ in size) and motile with a single polar flagellum. This strain was identified as a Pseudomonas sp. on the basis of its morphological, cultural and physiological characteristics. The strain showed a maximum productivity of phospholipid at $20^{\circ}C$ after 48 hours of culture time with an initia1 pH of 7.0 in the PYM-glucose medium. Under these culture conditions, the production of phospholipid was about 0.3 mg/ml and 0.06 mg/mg dry cell weight.

• Microbiology and Biotechnology Letters
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• v.17 no.3
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• pp.202-206
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• 1989

A microorganism, YS-221-B strain which powerfully produced $\alpha$-D-Glucosidase Inhibitor was isolated from soil. The morphological, cultural characteristics and physiological properties of the strain were investigated. As the results of various examinations, the strain YS-221-B was identified to be similar to Streptomyces flavouirens except the utilization of raffinose, i-inositol among the various carbon sources.

• Microbiology and Biotechnology Letters
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• v.22 no.4
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• pp.373-381
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• 1994

An actinomycete strain, HSL-613 was isolated -from soil and identified by International Streptomyces Project (ISP) and chemotaxonomic methods. The spore chain of the strain HSL-613 appears in a spiral shape, and its spores are spherical shape with smooth surface. The cell wall contains LL-diaminopimelic acid (DAP). Menaquinone MK-9 (H$_{6}$, H$_{8}$) and iso- and anteiso-branched fatty acids were detected from whole cell extract. Sugars identified from whole cell extract include galactose, glucose, mannose and ribose, which are distinct from general sugar patterns of Streptomyces. Average G+C content in the chromosome is 59%. 5S rRNA of HSL-613 consists of 120 nucleotides as determined by comparing with that of a type strain Streptomyces griseus subsp. KCTC 9080. Through morphological, physiological, and chemical characterization, HSL-613 was identified and named as Streptomyces sp. HSL-613.

• Journal of Ship and Ocean Technology
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• v.7 no.3
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• pp.56-65
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• 2003

The hull monitoring systems of container ships with four long-base gages give enough information for identifying the hull girder loads such as bending and torsional moments. But such a load-identification for container ships has not been known. In this paper, a load-identification method is suggested in terms of a linear matrix equation that the measured strain vector equals to the multiplication of the transformation matrix and the desired strain component vector. The equation is proved to be mathematically complete by the property of positive-definite determinant of the transformation matrix. The method is applied to a hull stress monitoring system for 8100TED container ship during sea trial, and the estimated external loads illustrate reasonable results in comparison with the pre-estimated results. This moment decomposition concept has also been tested in real operation conditions. The typical phenomena over the Suez Canal illustrated very suitable results comparing with the physical understandings. Henceforth, one can effectively use the proposed concept to monitor the hull girder loads such as bending and torsional moments.

• Journal of Microbiology and Biotechnology
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• v.25 no.8
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• pp.1257-1264
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• 2015

Trichoderma strain T-33 has been demonstrated to have inhibitory effect on the fungus species Cytospora chrysosperma. Here, an active antifungal compound was obtained from Trichoderma strain T-33 extract via combined separation technologies, including organic solvent extraction, liquid chromatography, and thin-layer chromatography. The purified compound was further characterized by advanced analytical technologies to elucidate its chemical structure. Results indicated that the active antifungal compound in Trichoderma strain T-33 extract is 2,5-cyclohexadiene-1,4-dione-2,6-bis (1,1-dimethylethyl).

• Journal of Korean Physical Therapy Science
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• v.9 no.1
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• pp.95-102
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• 2002

The present study focuses on the relation of the attitudes of university students to disabled by using modified disability factor scales(DFS, Siller). As for the attitude areas, it relies on the eight categories DFS provides. The 8 categories that specify each attitude area are: generalized rejection, distressed identification, inferred emotional consequences, imputed functional limitation, authoritarian virtuousness, rejection of intimacy, interaction strain, mainstreaming. This study is based on a series of questionnaire researches conducted on 322 students, over the period from October 19th to November 14th, 2001. The questionnaire carries 50 items, covering the 8 attitude areas and each item has 5 choices. The subjects' responses to each of the areas are examined in their relations to the four conditions, as they are analysed by t-test and one way ANOVA. The conclusion drawn from the research are as follows: 1. There is no significant difference between the students of the department concerned with the problem of disability, and those of the department unconcerned. But the responses are significantly different in the three areas of generalized rejection, rejection of intimacy and interaction strain(1st condition, P<.05). 2. The responses are significantly different in 6 areas except for the two, distressed identification and inferred emotional consequences, between the students who have volunteered services as helpers of the disabled and those who have no such experiences(2nd condition, P<.05). 3. the responses are significantly different between those who have volunteered long-time services and those who are far less experienced(3nd condition, P<.05). 4. There is no significant difference, whether the subjects have disabled persons around them or not. But generalized rejection, authoritarian virtuousness, rejection of intimacy, interaction strain & mainstreming show significant differences(4th condition, P<.05). 5. Generalized rejection, rejection intimacy and interaction strain show significant differences in every pair of the 4 condition. 6. Distressed identification and inferred emotional consequence show no significant difference between the two conditions in every pair of the 4 considerations.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.42 no.5
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• pp.461-467
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• 2009

A bacterial strain was screened and identified from sea sediments in Tongyeong coastal area in order to obtain proteases or peptidase cleaving C-terminal of glutamic acid. Strain TS0611, which showed the highest activity from 5 isolated protease producing strains, was selected and its properties investigated. Strain TS0611 was a gram-positive rod, $1.2\;{\mu}m$ in cell length, catalase positive, motility-positive, melanin-negative and grew at 15~$50^{\circ}C$, and hydrolyzed gelatin and casein. This strain was identified as Bacillus thuringiensis or Bacillus cereus based on results from the API system(API 50 CHB) which examined saccharides properties, fatty acid composition of cell wall, and 16S rRNA gene sequence.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.49 no.5
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• pp.573-581
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• 2016

Bacterial decarboxylation of amino acids in food leads to the production of biogenic amines, which can cause reactions in human that include headaches, nausea, palpitations, chills, and severe respiratory distress. The amine putrescine is an especially effective inhibitor of metabolizing enzymes and amplifies histamine intoxication and tyramine poisoning. Using an L-ornithine decarboxylating medium, we isolated 14 putrescine-producing bacteria from sand lance, Ammodytes personatus, sauces. The isolates were identified, using an API kit and 16S rRNA analysis, as Lysinibacillus fusiformis (1 strain), Lysinibacillus xylanilyticus (6 strains), Lysinibacillus macroides (1 strain), Lysinibacillus sphaericus (3 strains), Bacillus fusiformis (1 strain), Paenibacillus favisporus (1 strain), and Staphylococcus caprae (1 strain). These strains produced between 1.66 to 236.97 μg/mL of putrescine after 48 h incubation. Lysinibacillus spp. were the dominant putrescine-producing bacteria in sand lance sauces, which produced 236.97 μg/mL of putrescine from a culture broth containing 0.5% L-ornithine. This is the first report on the isolation and identification of putrescine-producing bacteria from sand lance sauces.

• Journal of Microbiology
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• v.34 no.2
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• pp.124-130
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• 1996

A survey was conducted to determine the prevalance of infectious pancreatic necrosis virus (IPNV) on fish farms in Korea and the epidemiology of IPNV infection in the farmed rainbow trout. In total, 43 pools of rainbow trout with apparent signs of viral infection from five provinces were obtained and analyzed. Evident cytopathic effects, including karyopycnosis and cell destruction, were observed in CHSE (chinook samlmon embyro)-214 cells infected with the virus isolates. Of these, ten viral isolates were assumed to be IPNV based on biophysical properties. RNA analysis revealed that the isolates contained two-segmented RNA genomes, further indicating that the viral isolates are IPNV. Antigenic comparison of the IPNV isolates identified three distinct serological groups separable by the cross-neutralization test. Of the ten IPNV isolates, six could be classified as strain DRT, two as strain Ab, and two as strain VR299. We were not able to isolate new strain of IPNV or any isolate serologically similar to the standard strain Sp.poraceae and families of the Agaricales, they are genetically more related to the Polyporaceae. These results are consistent with morphological characters observed in those mushrooms. However, it is premature to conclude taxonomic status Ganoderma species in the present study employing small sample size.

• Korean Journal of Microbiology
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• v.30 no.5
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• pp.415-420
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• 1992

Numerical identification was carried out for an isolate of Streptomyces strain producing the extracellular .betha.-lactamase inhibitor. Fifty taxonbomic unit characters were tested and the data were analyzed numerically using the TAXON program. The isoalte was identified to the majro cluster 5 of Streptomyces and it was best matched to Strepstomyces omiyaensis which is a synonym of Streptomyces exfoliatus. Therefore, it was concluded that the isolate was identified to be a strain (SMF19) of Streptomyces exfoliatus.