• 제목/요약/키워드: strain developments

검색결과 39건 처리시간 0.023초

바퀴(Blattella germanica L.)의 살충제 저항성에 관한 연구. 2. 저항성 발현 및 교차저항성 (Studies on the Insecticide Resistance of the German Cockroach (Blattella germanica L.) II. Resistant Developments and Cross resistance)

  • 방종렬;이형래;김정화
    • 한국응용곤충학회지
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    • 제32권2호
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    • pp.129-133
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    • 1993
  • 바퀴(Blattella germanica L.)를 chlorpyrifos와 permthrin 살충제로 누대도태하여 얻어진 저항성 바퀴를 대상으로 저항성 유발 및 교차저항성에 대해서 조사한 결과는 다음과 같다. chlorpyrifos와 permethrin으로 6세대 도태 한 결과 감수성 계통 보다 도태계통의 반수치사농도가 각각 3.23배, 2.10배 증가하였다. chlorpyrifos도태계통에 있어서 propoxur, fenvalerate 및 permethrin의 반수치사농도가 각각 2.97배, 3.89배 및 1.31배로써 fenvalerate 와 proposur가 permethrin보다 비교적 높은 교차저항성을 보였으며, permethrin 도태계통에 있어서 chorpyrifos, propoxur 및 fenvalerate의 반수치사농도가 각각 1.42배, 1.08배 및 5.23배로써 fenvalerate가 다른 살충제에 비하여 비교적 높은 교차저항성을 보였다.

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Evaluation of Mungbean Genotypes Based on Yield Stability and Reaction to Mungbean Yellow Mosaic Virus Disease

  • Alam, A.K.M. Mahbubul;Somta, Prakit;Jompuk, Choosak;Chatwachirawong, Prasert;Srinives, Peerasak
    • The Plant Pathology Journal
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    • 제30권3호
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    • pp.261-268
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    • 2014
  • This work was conducted to identify mungbean genotypes showing yield stability and resistance to mungbean yellow mosaic virus (MYMV) disease. Sixteen genotypes were evaluated in a randomized complete block design with two replications for two years (2011 and 2012) at three locations (Gazipur, Ishurdi and Madaripur) of the Bangladesh Agricultural Research Institute. An analysis of variance exhibited significant effects of genotype (G), environment (E), and genotype ${\times}$ environment ($G{\times}E$) on grain yield. Among eight agronomic characters, the principal component 1 (PC1) was always higher than the PC2. Considering $G{\times}E$ interaction, BM6 was the best genotype at all three locations in both years. Based on grain yield and stability performance, BM6 ranked first while the worst performing genotypes were BM1 and G10. Based on discrimination and representation, Gazipur was identified as an ideal environment for these mungbeans. Relationship between soil-plant analysis developments (SPAD) value was positive with yield but negative with MYMV severity. BM6, G1 and G2 were considered as promising sources of resistance for low disease score and stable response across the environments. The environment proved to have an influence on MYMV infection under natural infestation. A positive correlation was observed between disease score and the temperature under natural growing condition.

통합 구조 시스템의 유한요소망 형성의 자동화 (Automated Finite Element Mesh Generation for Integrated Structural Systems)

  • 윤종열
    • 한국지진공학회논문집
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    • 제27권2호
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    • pp.77-82
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    • 2023
  • The structural analysis module is an essential part of any integrated structural system. Diverse integrated systems today require, from the analysis module, efficient real-time responses to real-time input such as earthquake signals, extreme weather-related forces, and man-made accidents. An integrated system may also be for the entire life span of a civil structure conceived during the initial conception, developed throughout various design stages, effectively used in construction, and utilized during usage and maintenance. All these integrated systems' essential part is the structural analysis module, which must be automated and computationally efficient so that responses may be almost immediate. The finite element method is often used for structural analysis, and for automation, many effective finite element meshes must be automatically generated for a given analysis. A computationally efficient finite element mesh generation scheme based on the r-h method of mesh refinement using strain deviations from the values at the Gauss points as error estimates from the previous mesh is described. Shape factors are used to sort out overly distorted elements. A standard cantilever beam analyzed by four-node plane stress elements is used as an example to show the effectiveness of the automated algorithm for a time-domain dynamic analysis. Although recent developments in computer hardware and software have made many new applications in integrated structural systems possible, structural analysis still needs to be executed efficiently in real-time. The algorithm applies to diverse integrated systems, including nonlinear analyses and general dynamic problems in earthquake engineering.

보수/보강 효과를 고려한 철근콘크리트교량의 내진응답해석 (Earthquake Response Analysis of a RC Bridge Including the Effect of Repair/retrofitting)

  • 이도형;조규상;전정문
    • 대한토목학회논문집
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    • 제28권4A호
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    • pp.611-622
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    • 2008
  • 보수된 교량 교각의 보수 후의 내진응답 거동을 검토하기 위하여 보수요소를 이용하여 교각 및 교량구조물에 대한 비선형해석을 수행하였다. 본 연구목적을 위하여 단순화된 CFRP 재료의 응력-변형률 모델을 제안하였다. 본 연구에서 제안된 해석기법 및 모델링을 포함한 교각의 해석결과는 강도와 강성에 있어서 실험결과와 만족할 만한 상관관계를 나타내었다. 또한, 교각의 보수효과가 교량전체구조물에 미치는 영향을 검토하기 위하여 다양한 연속지진하중 조합을 작용시켜 교량구조물의 동적시간이력해석을 수행하였다. 이 해석에서는 교각의 이론적인 소성힌지 구간을 CFRP와 강판피복으로 보수하였고, 비교해석결과는 보수 후의 응답을 위해 보수효과를 직접적으로 반영한 해석의 필요성을 입증해 주었다. 결론적으로 본 연구에서 제안된 해석기법 및 모델은 손상된 구조물의 내진보수와 관련하여 유용한 정보를 제공해 줄 수 있을 것으로 사료된다.

혼합구조의 성능 향상을 위한 개선된 접합부의 개발 (II): 개선된 접합방식의 성능확인을 위한 모형실험 및 해석 (Developments of Advanced Connection Type for Improvements of Mixed Structures (II))

  • 윤익중;노병철;김문겸;조성용
    • 대한토목학회논문집
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    • 제28권2A호
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    • pp.207-214
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    • 2008
  • 본 논문에서는 연계논문에서 제안된 혼합구조 접합부의 개선정도를 확인하기 위하여 2개의 혼합구조 실험체에 대하여 4절점 휨시험을 수행하였다. 혼합구조 접합부의 거동 분석을 위하여 3차원 비선형해석 결과와 실재하 실험 결과를 하중-처짐 관계, 하중-변형률 관계, 접합부 개구폭, 균열과 파괴모드를 통하여 비교하였다. 3차원 비선형 해석을 위하여 접촉요소를 사용하였으며 해석프로그램은 범용 구조 해석프로그램인 아바쿠스를 이용하였다. 실험과 해석의 결과로부터 제안된 L 모양의 접합부가 휨하중에서 기존안보다 강성이 크게 나타났으며, 보다 나은 구조적 성능을 나타냄을 확인하였다.

프로바이오틱 제품 개발 동향과 과제 (Development of Probiotic Products and Challenges)

  • 서재구;이과수;김진응;정명준
    • KSBB Journal
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    • 제25권4호
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    • pp.303-310
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    • 2010
  • 프로바이오틱스는 위장관에서 고유의 생리활성을 발휘하기 때문에 섭취 후 최대한 장까지 사멸하지 않고 도달하도록 하는 코팅이나 encapsulation 기술이 개발되어 왔다. 이와 함께 제품의 형태도 여러 종류의 유산균이 포함된 multispecies probiotics가 선호되는 추세이며 높은 생균수를 가지는 제품들이 개발되고 있다. 프로바이오토틱 시장은 현재까지 생균 중심의 제품들이 대부분을 차지하고 있으나 사균체 제품 또는 박테리오신과 같은 항균물질을 포함하는 제품 또한 다양한 분야에 적용될 수 있고 생균제와 비교하여 여러가지 장점을 가지고 있어 관련 제품개발이 이루어져야 할 것으로 보인다. 프로바이오틱 제품들은 살아 있는 생균제로서 질환특이적 효능을 바탕으로 개발되고 있으나 질환개선 효능 표시에 대한 규제가 강화되고 있어 이에 대한 대비가 필요하다. 이를 위해 제품에 사용된 strain의 동정, 안전성 및 기능성에 대한 과학적 증거가 제시되어야 할 것이며 임상을 통한 안전성과 효능에 대한 검증 자료가 필요할 것으로 보인다. 이와 같은 프로바이오틱스 제품의 헬스클레임에 대한 엄격해진 검증은 앞으로 프로바이오틱 시장에 큰 영향을 미칠 것으로 판단된다.

An exact finite strip for the calculation of relative post-buckling stiffness of isotropic plates

  • Ovesy, H.R.;Ghannadpour, S.A.M.
    • Structural Engineering and Mechanics
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    • 제31권2호
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    • pp.181-210
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    • 2009
  • This paper presents the theoretical developments of an exact finite strip for the buckling and initial post-buckling analyses of isotropic flat plates. The so-called exact finite strip is assumed to be simply supported out-of-plane at the loaded ends. The strip is developed based on the concept that it is effectively a plate. The present method, which is designated by the name Full-analytical Finite Strip Method in this paper, provides an efficient and extremely accurate buckling solution. In the development process, the Von-Karman's equilibrium equation is solved exactly to obtain the buckling loads and the corresponding form of out-of-plane buckling deflection modes. The investigation of thin flat plate buckling behavior is then extended to an initial post-buckling study with the assumption that the deflected form immediately after the buckling is the same as that obtained for the buckling. It is noted that in the present method, only one of the calculated out-of-plane buckling deflection modes, corresponding to the lowest buckling load, i.e., the first mode is used for the initial post-buckling study. Thus, the postbuckling study is effectively a single-term analysis, which is attempted by utilizing the so-called semi-energy method. In this method, the Von-Karman's compatibility equation governing the behavior of isotropic flat plates is used together with a consideration of the total strain energy of the plate. Through the solution of the compatibility equation, the in-plane displacement functions which are themselves related to the Airy stress function are developed in terms of the unknown coefficient in the assumed out-of-plane deflection function. These in-plane and out-of-plane deflected functions are then substituted in the total strain energy expressions and the theorem of minimum total potential energy is applied to solve for the unknown coefficient. The developed method is subsequently applied to analyze the initial postbuckling behavior of some representative thin flat plates for which the results are also obtained through the application of a semi-analytical finite strip method. Through the comparison of the results and the appropriate discussion, the knowledge of the level of capability of the developed method is significantly promoted.

표고버섯의 원형질체 분리 최적화와 RNPs/나노파티클 복합체 형성 (Optimization of Protoplast Isolation and Ribonucleoprotein/Nanoparticle Complex Formation in Lentinula edodes)

  • 김민식;류호진;오민지;임지훈;이종원;오연이
    • 한국버섯학회지
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    • 제20권3호
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    • pp.178-182
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    • 2022
  • 버섯의 오랜 역사에도 불구하고 버섯의 유전적 기능과 분자유전학을 응용한 신품종 개발에 대한 연구는 크게 부족한 상황이다. 그러나 최근 유전자 가위인 CRISPR/Cas를 이용한 새로운 유전자 교정 기술이 개발됨에 따라 버섯 연구에서 이 기술을 이용한 다양한 시도가 이루어지고 있다. 특히 선택의 용이성을 위해 외래 유전자 삽입 없이도 고효율로 유전자 편집이 가능한 RNPs를 활용한 연구가 활발히 진행되고 있다. 그러나 RNPs는 원형질체의 세포막을 통과하기에 Cas9이 너무 거대하고 guide RNA가 쉽게 파괴된다는 단점을 가지고 있다. 이러한 단점을 극복하기 위하여 세포막 통과에 용이한 미네랄 성분인 CaP와 PAA를 조합하여 Nanoparticle을 형성함으로써 극복하고자 했다. 표고버섯 단핵 균주인 산조705-13을 이용하여 원형질체 분리에 적합한 Osmotic buffer를 찾기 위하여 0.6M과 1.2M의 Sucrose, Sorbitol, Mannitol, KCl을 처리하였고 그 결과 0.6M Sucrose가 가장 적합한 osmotic buffer임을 확인하였다. 또한 CaP으로 RNPs와 Nanoparticle 복합체를 형성하고 이 복합체가 RNase A로부터 RNPs의 기능을 온전히 보호하는 것을 확인할 수 있었다.

Beak Trimming Methods - Review -

  • Glatz, P.C.
    • Asian-Australasian Journal of Animal Sciences
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    • 제13권11호
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    • pp.1619-1637
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    • 2000
  • A review was undertaken to obtain information on the range of beak-trimming methods available or under development. Beak-trimming of commercial layer replacement pullets is a common yet critical management tool that can affect the performance for the life of the flock. The most obvious advantage of beak-trimming is a reduction in cannibalism although the extent of the reduction in cannibalism depends on the strain, season, and type of housing, flock health and other factors. Beak-trimming also improves feed conversion by reducing food wastage. A further advantage of beak-trimming is a reduction in the chronic stress associated with dominance interactions in the flock. Beak-trimming of birds at 7-10 days is favoured by Industry but research over last 10 years has shown that beak-trimming at day-old causes the least stress on birds and efforts are needed to encourage Industry to adopt the practice of beak-trimming birds at day-old. Proper beak-trimming can result in greatly improved layer performance but improper beak-trimming can ruin an other wise good flock of hens. Re-trimming is practiced in most flocks, although there are some flocks that only need one trimming. Given the continuing welfare scrutiny of using a hot blade to cut the beak, attempts have been made to develop more welfare friendly methods of beak-trimming. Despite the developments in design of hot blade beak-trimmers the process has remained largely unchanged. That is, a red-hot blade cuts and cauterises the beak. The variables in the process are blade temperature, cauterisation time, operator ability, severity of trimming, age of trimming, strain of bird and beak length. This method of beak-trimming is still overwhelmingly favoured in Industry and there appears to be no other alternative procedures that are more effective. Sharp secateurs have been used trim the upper beak of both layers and turkeys. Bleeding from the upper mandible ceases shortly after the operation, and despite the regrowth of the beak a reduction of cannibalism has been reported. Very few differences have been noted between behaviour and production of the hot blade and cold blade cut chickens. This method has not been used on a large scale in Industry. There are anecdotal reports of cannibalism outbreaks in birds with regrown beaks. A robotic beak-trimming machine was developed in France, which permitted simultaneous, automated beak-trimming and vaccination of day-old chicks of up to 4,500 chickens per hour. Use of the machine was not successful because if the chicks were not loaded correctly they could drop off the line, receive excessive beak-trimming or very light trimming. Robotic beak-trimming was not effective if there was a variation in the weight or size of chickens. Capsaicin can cause degeneration of sensory nerves in mammals and decreases the rate of beak regrowth by its action on the sensory nerves. Capsaicin is a cheap, non-toxic substance that can be readily applied at the time of less severe beak-trimming. It suffers the disadvantage of causing an extreme burning sensation in operators who come in contact with the substance during its application to the bird. Methods of applying the substance to minimise the risk to operators of coming in contact with capsaicin need to be explored. A method was reported which cuts the beaks with a laser beam in day-old chickens. No details were provided on the type of laser used, or the severity of beak-trimming, but by 16 weeks the beaks of laser trimmed birds resembled the untrimmed beaks, but without the bill tip. Feather pecking and cannibalism during the laying period were highest among the laser trimmed hens. Currently laser machines are available that are transportable and research to investigate the effectiveness of beak-trimming using ablasive and coagulative lasers used in human medicine should be explored. Liquid nitrogen was used to declaw emu toes but was not effective. There was regrowth of the claws and the time and cost involved in the procedure limit the potential of using this process to beak-trim birds.