• Title/Summary/Keyword: strain development

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Microstructure Evolution of 15Cr ODS Steel by a Simple Torsion Test (단순 전단변형에 의한 15Cr 산화물 분산강화 강의 미세조직 변화)

  • Jin, Hyun Ju;Kang, Suk Hoon;Kim, Tae Kyu
    • Journal of Powder Materials
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    • v.21 no.4
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    • pp.271-276
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    • 2014
  • 15Cr-1Mo base oxide dispersion strengthened (ODS) steel which is considered to be as a promising candidate for high- temperature components in nuclear fusion and fission systems because of its excellent high temperature strength, corrosion and radiation resistance was fabricated by using mechanical alloying, hot isostatic pressing and hot rolling. Torsion tests were performed at room temperature, leading to two different shear strain routes in the forward and reverse directions. In this study, microstructure evolution of the ODS steel during simple shearing was investigated. Fine grained microstructure and a cell structure of dislocation with low angle boundaries were characterized with shear strain in the shear deformed region by electron backscattered diffraction (EBSD). Grain refinement with shear strain resulted in an increase in hardness. After the forward-reverse torsion, the hardness value was measured to be higher than that of the forward torsion only with an identical shear strain amount, suggesting that new dislocation cell structures inside the grain were generated, thus resulting in a larger strengthening of the steel.

Development of Fenvalerate Resistance in the Diamondback Moth, Plutella xylostela Linne (Lepidoptera : Yponomeutidae) and its Cross Resistance (배추좀나방의 Fenvalerate에 대한 저항성 발달과 교차저항성)

  • 김길하;서영식;이준호;조광연
    • Korean journal of applied entomology
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    • v.29 no.3
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    • pp.194-200
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    • 1990
  • The diamondback moth (Plutella xylostella L.) was selected over 24 generations with fenvalerate. The resulting resistant strain was tested to study development of insecticide resistance and cross resistance to some insecticides in the laboratory. Insecticide resistance of diamondback moth at the 24th generation devleoped 66.2 fold compared to the parent strain for fenvalerate. The fenvalerate selected strain exhibited 145 fold, a high level of cross resistance to deltamethrin, and also showed 17.4-45.0 fold cross resistance to alphamethrin, cypermethrin, fenvalerate, permethrin, and tetramethrin in the pyrethroid insecticides. The fenvalerate selected strain showed 2.5-4.3 fold, low cross resistance to diazinon, dichlorvos, EPN, BPMC, cabaryl, and methomyl. However, it did not show cross resistance to acephate, fenitrothion, phenthoate, and carbofuran.

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Current Status and Applications of Adaptive Laboratory Evolution in Industrial Microorganisms

  • Lee, SuRin;Kim, Pil
    • Journal of Microbiology and Biotechnology
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    • v.30 no.6
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    • pp.793-803
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    • 2020
  • Adaptive laboratory evolution (ALE) is an evolutionary engineering approach in artificial conditions that improves organisms through the imitation of natural evolution. Due to the development of multi-level omics technologies in recent decades, ALE can be performed for various purposes at the laboratory level. This review delineates the basics of the experimental design of ALE based on several ALE studies of industrial microbial strains and updates current strategies combined with progressed metabolic engineering, in silico modeling and automation to maximize the evolution efficiency. Moreover, the review sheds light on the applicability of ALE as a strain development approach that complies with non-recombinant preferences in various food industries. Overall, recent progress in the utilization of ALE for strain development leading to successful industrialization is discussed.

Genome analysis of Bacteroides sp. CACC 737 isolated from feline for its potential application

  • Kim, Jung-Ae;Jung, Min Young;Kim, Dae-Hyuk;Kim, Yangseon
    • Journal of Animal Science and Technology
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    • v.62 no.6
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    • pp.952-955
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    • 2020
  • Bacteroides sp. CACC 737 was isolated from a feline, and its potential probiotic properties were characterized using functional genome analysis. Whole-genome sequencing was performed using the PacBio RSII and Illumina HiSeq platforms. The complete genome of strain CACC 737 contained 4.6 Mb, with a guanine (G) + cytosine (C) content of 45.8%, six cryptic plasmids, and extracellular polysaccharide gene as unique features. The strain was beneficial to animal health when consumed as feed, for example, for ameliorating immunological dysfunctions and metabolic disorders. The genome information adds to the comprehensive understanding of Bacteroides sp. and suggests potential animal-related industrial applications for this strain.

Studies of the Insecticide Resistance in the Green Peach Aphid, Myzus persicae Sulzer (V). Development of Cypermethrin and Pirimicarb Resistance, and Cross Resistance (복숭아혹진딧물의 살충제 저항성에 관한 연구 (V). Cypermethrin과 Pirimicarb에 의한 저항성 발달과 교류저항성)

  • 최승윤;김길하;안용준
    • Korean journal of applied entomology
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    • v.28 no.1
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    • pp.23-27
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    • 1989
  • The green peach aphid(Myzus pericae Sulzer) was selected over 20 generations with cypermethrin and pirmicarb, respectively. The resulting resistant strains were tested to inverting-ate the development of insecticide resistance and cross-resistance to some insecticides in the laboratory. The development of insecticide resistance against green peach aphid at the 20th selected generation was greatly varied with the insecticides: 20.5 fold for cypermethrin and 3.2 fold for pirimicarb compared with the parent strain. The cypermethrin selected strain exhibited cross resistance to acephate and pirimicarb, and pirimicarb selected strain to acephate and cypermethrin, respectively. Demeton-S-methyl, however, has not been shown cross-resistance by the selected strains.

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Microstructural Evolution during Hot Deformation of Molybdenum using Processing Map Approach (변형지도 모델링을 통한 몰리브데늄의 고온 변형에 따른 미세조직 변화 연구)

  • Kim, Young-Moo;Lee, Sung-Ho;Lee, Seong;Noh, Joon-Woong
    • Journal of Powder Materials
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    • v.15 no.6
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    • pp.458-465
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    • 2008
  • The hot deformation characteristics of pure molybdenum was investigated in the temperature range of $600{\sim}1200^{\circ}C$ and strain rate range of $0.01{\sim}10.0/s$ using a Gleeble test machine. The power dissipation map for hot working was developed on the basis of the Dynamic Materials Model. According to the map, dynamic recrystallization (DRX) occurs in the temperature range of $1000{\sim}1100^{\circ}C$ and the strain rate range of $0.01{\sim}10.0/s$, which are the optimum conditions for hot working of this material. The average grain size after DRX is $5{\mu}m$. The material undergoes flow instabilities at temperatures of $900{\sim}1200^{\circ}C$ and the strain rates of $0.01{\sim}10.0/s$, as calculated by the continuum instability criterion.

Prediction of Liquation Crack Initiation at HAZ of Laser Weldment Based on Strain Analysis at Elevated Temperature

  • Yamamoto, Motomichi;Shinozaki, Kenji;Kitamura, Mitsuru;Shirai, Makoto
    • Proceedings of the KWS Conference
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    • 2002.10a
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    • pp.244-249
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    • 2002
  • The purpose of this study is to develope the prediction method of liquation crack initiation in HAZ of laser weldment. Thermal two dimensional strain analyses were performed by FEM for bead-on-plate welding in order to obtain the plastic strain at elevated temperature in HAZ of the laser weldment. From these results, it became clear that the plastic strain at elevated temperature affected liquation crack initiation in HAZ, and it could be proposed that the critical strain, which controlled liquation crack initiation, existed. Moreover, an attempt was made to develop thermal and dynamic three dimensional strain analysis method for the laser weldment in order to obtain the plastic strain at elevated temperature in HAZ of the laser weldment in more detail and precisely.

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Hot Deformation Behavior and Microstructural Evolution of Powder Metallurgy Ti-6Al-4V Alloy (티타늄 합금 분말 소결체의 고온 변형 거동 및 미세조직 연구)

  • Kim, Youngmoo;Song, Young-Beom;Lee, Sung Ho;Kwon, Young-Sam
    • Journal of Powder Materials
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    • v.21 no.4
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    • pp.277-285
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    • 2014
  • The effects of processing parameters on the flow behavior and microstructures were investigated in hot compression of powder metallurgy (P/M) Ti-6Al-4V alloy. The alloy was fabricated by a blended elemental (B/E) approach and it exhibited lamellar ${\alpha}+{\beta}$ microstructure. The hot compression tests were performed in the range of temperature $800-1000^{\circ}C$ with $50^{\circ}C$ intervals, strain rate $10^{-4}-10s^{-1}$, and strain up to 0.5. At $800-950^{\circ}C$, continuous flow softening after a peak stress was observed with strain rates lower than $0.1s^{-1}$. At strain rates higher than $1s^{-1}$, rapid drop in flow stress with strain hardening or broad oscillations was recorded. The processing map of P/M Ti-6Al-4V was designed based on the compression test and revealed the peak efficiency at $850^{\circ}C$ and $0.001s^{-1}$. As the processing temperature increased, the volume fraction of ${\beta}$ phase was increased. In addition, below $950^{\circ}C$, the globularization of phase at the slower strain rate and kinking microstructures were found. Based on these data, the preferred working condition of the alloy may be in the range of $850-950^{\circ}C$ and strain rate of $0.001-0.01s^{-1}$.

Suitability of Lactobacillus plantarum SPC-SNU 72-2 as a Probiotic Starter for Sourdough Fermentation

  • Park, Da Min;Bae, Jae-Han;Kim, Min Soo;Kim, Hyeontae;Kang, Shin Dal;Shim, Sangmin;Lee, Deukbuhm;Seo, Jin-Ho;Kang, Hee;Han, Nam Soo
    • Journal of Microbiology and Biotechnology
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    • v.29 no.11
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    • pp.1729-1738
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    • 2019
  • In sourdough fermentation, lactic acid bacteria perform important roles in the production of volatile and antimicrobial compounds, and exerting health-promoting effects. In this study, we report the probiotic properties and baking characteristics of Lactobacillus plantarum SPC-SNU 72-2 isolated from kimchi. This strain is safe to use in food fermentation as it does not carry genes for biogenic amine production (i.e., hdc, tdc, and ldc) and shows no β-hemolytic activity against red blood cells. The strain is also stable under simulated human gastrointestinal conditions, showing tolerance to gastric acid and bile salt, and adheres well to colonic epithelial cells. Additionally, this strain prevents pathogen growth and activates mouse peritoneal macrophages by inducing cytokines such as tumor necrosis factor-α, interleukin (IL)-6, and IL-12. Furthermore, the strain possesses good baking properties, providing rich aroma during dough fermentation and contributing to the enhancement of bread texture. Taken together, L. plantarum SPC-SNU 72-2 has the properties of a good starter strain based on the observation that it improves bread flavor and texture while also providing probiotic effects comparable with commercial strains.