• Title/Summary/Keyword: strain demand

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Isolation and Characterization of Terephthalic Acid-degrading Bacteria (Terephthalic Acid 분해 세균의 분리 및 특성)

  • 김재화;이창호;우철주;주길재;서승교;박희동
    • Microbiology and Biotechnology Letters
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    • v.27 no.2
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    • pp.118-123
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    • 1999
  • A bacterial strain, designated T116, degrading terephthalic acid (TPA) was isolated from the soil around Taegu industrial area into which dye works wastewater flow. The isolate was identified as pseudomonas sp. based on its morphological and physiological characteristics. Degradation of TPA by the strain T116 was confirmed with UV scanning and HPLC. About 90% and 98% of TPA were degraded after 36 and 60 hours, respectively, during the culture in a liquid medium containing 0.1% TPA. Addition of KH2PO4 at a final concentration of 100ppm enhanced the chemical oxygen demand (COD) removal rate about 50% from dye works wastewater by Pseudomonas sp. T116. Optimum pH and temperature for COD reduction from wastewater were 7.0 and 3$0^{\circ}C$, respectively. The bacterium was applied to the continuous culture for the treatment of dye works wastewater whose TPA concentration and CODMn were 2,200ppm and 1,620ppm, respectively. It was observed that 90-95% of COD was eliminated after 4 days culture in the continuous culture with a retention time of 37 or 47 hours.

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A Study on Job Stress of Container Termainal Workers (항만하역 근로자들의 직무 스트레스에 관한 연구)

  • Choi, Eun-Kyung;Kim, Kong-Hyun;Lee, Jong-Tae
    • Korean Journal of Occupational Health Nursing
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    • v.11 no.1
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    • pp.63-80
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    • 2002
  • Objectives: The objective of this study is to evaluate the job characteristics of container terminal workers by applying the Job Strain model, and to assess the relationship among the general characteristics, job characteristics and psychosocial distress. Methods: A self-administrated questionnaire survey was performed to the container terminal workers in Pusan. Among the 200 male workers who answered the questionnaires, white-collar workers and blue-collar workers were 100, respectively. Karaseks Job Content Questionnaire was utilized to evaluate the job characteristics and Psychosocial well-being index (PWI) was applied to measure the extent of their psychosocial stress. Results: In white-collar workers, the skill discretion, created skill, decision-making authority, decision-making latitude, psychological job demand, and supervisor support of the job characteristics were significantly high, while in blue-collar workers physical exertion was significantly high. The result of Psychosocial well-being index (PWI) reveals that blue-collar workers were more stressed than white-collar workers, especially, the indices of depression (factor 2), sleeping disturbance and anxiety (factor 3), General well-being and vitality (factor 4) were significantly increased; whereas, in white-collar workers, only the index of social performance and self-confidence (factor 1) was significantly increased. And PWI scores were significantly increased in the lower social support and psychological job demand. By the multiple logistic regression analysis for PWI, blue-collar workers had increased odds ratio of 2.66(95% CI;1.11-6.41) compared with white-collar workers. The unmarried workers increased odds ratio of 3.54(95% CI;1.18-10.62) compared with married workers. And workers who have not own house increased odds ratio of 2.35(95% CI;1.15-4.79) compared with workers who have own house. Particularly, odds ratio of work-shift in blue-collar workers was 11.10(2.14-57.64). Conclusion: Skill discretion, created skill, decision-making authority, decision-making latitude, psychological job demand, and supervisor support were increased in white-collar workers. Decreased skill discretion and increased physical exertion were found in blue-collar workers, which is supported the Job Strain model. Job stress of blue-collar workers was comparatively higher than that of white-collar workers, especially, skill discretion, decision-making authority, decision-making latitude, job insecurity, physical exertion were noticeable factors. Especially, sleeping, smoking, and work shifting turned out to be a main cause that increases stress. Therefore, in order to decrease the job stress, a health promotion program to change the health behaviors should be activated and an organized job stress management program should be introduced. Especially, working condition for blue-collar such as physical exertion and work-shift should be improved.

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Implications of yield penetration on confinement requirements of r.c. wall elements

  • Tastani, Souzana P.;Pantazopoulou, Stavroula J.
    • Earthquakes and Structures
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    • v.9 no.4
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    • pp.831-849
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    • 2015
  • Seismic-design procedures for walls require that the confinement in the critical (plastic hinge) regions should extend over a length in the compression zone of the cross section at the wall base where concrete strains in the Ultimate Limit State (ULS) exceed the limit of 0.0035. In a performance-based framework, confinement is linked to required curvature ductility so that the drift demand at the performance point of the structure for the design earthquake may be met. However, performance of flexural walls in the recent earthquakes in Chile (2010) and Christchurch (2011) indicates that the actual compression strains in the critical regions of many structural walls were higher than estimated, being responsible for several of the reported failures by toe crushing. In this study, the method of estimating the confined region and magnitude of compression strain demands in slender walls are revisited. The objective is to account for a newly identified kinematic interaction between the normal strains that arise in the compression zone, and the lumped rotations that occur at the other end of the wall base due to penetration of bar tension yielding into the supporting anchorage. Design charts estimating the amount of yield penetration in terms of the resulting lumped rotation at the wall base are used to quantify the increased demands for compression strain in the critical section. The estimated strain increase may exceed by more than 30% the base value estimated from the existing design expressions, which explains the frequently reported occurrence of toe crushing even in well confined slender walls under high drift demands. Example cases are included in the presentation to illustrate the behavioral parametric trends and implications in seismic design of walls.

Measurement of Thermal Expansion Coefficient of Rock using Strain Gauge (스트레인 게이지를 이용한 암석의 열팽창계수 측정)

  • Park, Chan;Kim, Hyung-Mok;Synn, Joong-Ho;Park, Yeon-Jun;Cheon, Dae-Sung
    • Tunnel and Underground Space
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    • v.17 no.6
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    • pp.475-483
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    • 2007
  • With increasing demand for LNG as energy resources and need for $CO_2$ sequestration as greenhouse gas, more storage facilities are required in Korea. Due to the recent acute safety concerns and land shortage, storage facilities tend to be located underground. In design and construction of underground storage for low and high temperature materials, besides their mechanical characteristics, the thermal characteristics of rock under temperature variation should be understood. In this study, laboratory experiments for the measurement of the thermal expansion coefficient of rock were performed using strain gauge in consideration of the particle size of mineral and experiment temperature range. Experiment results show that thermal expansion coefficient decreased as the temperature decreases. In addition, linear thermal expansion coefficient was developed for typical Korean rocks such as granite. The results of this study can be utilized for the evaluation of thermal propagation in rock mass and the thermo-mechanical stability of underground facilities.

Cultural Performances of Two Escherichia coli Host- vector Systems for Production of $\beta$-Galactosidase ($\beta$-Galactosidase 생산을 위한 두 대장균 숙주-벡터의 배양 특성)

  • Choi, D.K;Park, Y.H.
    • Microbiology and Biotechnology Letters
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    • v.15 no.6
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    • pp.396-401
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    • 1987
  • Protein productivities of a cloned gene ($\beta$-galactosidase) and the cultural performances of two recombinant Escherichia coli strains, which use different host-vector systems, were studied. E. coli JM109/pTBG10 strain which carries Tac promoter had higher protein productivity than E. coli MH3000 (pRKc1857)/pASI(lacZ) strain which carries pL promoter. Induction of protein syn-thesis was optimum at the initial-and mid-logarithmic growth phases for both strains. Oxygen demand was observed to be very high during the cloned gene expression, and could be alleviated to some extent through pH control. The ratio of specific growth rates of plasmid-harboring to plasmidfree cell, $\mu$+ /$\mu$-, of the high productivity strain was observed to be lower than that of the low productivity one. Plasmid stability was analyzed for 20-30 generations, and it was found that the traction of plasmid-harboring cells dropped to l0% level in about 25 generations for both strains when the cloned gene expression was induced.

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Experimental Study on Unconfined Compression Strength and Split Tensile Strength Properties in relation to Freezing Temperature and Loading Rate of Frozen Soil (동결 온도와 재하속도에 따른 동결토의 일축압축 및 쪼갬인장 강도특성)

  • Seo, Young-Kyo;Choi, Heon-Woo
    • Journal of Ocean Engineering and Technology
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    • v.26 no.6
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    • pp.19-26
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    • 2012
  • Recently the world has been suffering from difficulties related to the demand and supply of energy due to the democratic movements sweeping across the Middle East. Consequently, many have turned their attention to never-developed extreme regions such as the polar lands or deep sea, which contain many underground resources. This research investigated the strength and initial elastic modulus values of eternally frozen ground through a uniaxial compression test and indirect tensile test using frozen artificial soil specimens. To ensure accurate test results, a sandymud mixture of standard Jumunjin sand and kaolinite (20% in weight) was used for the specimens in these laboratory tests. Specimen were prepared by varying the water content ratio (7%, 15%, and 20%). Then, the variation in the strength value, depending on the water content, was observed. This research also established three kinds of environments under freezing temperatures of $-5^{\circ}C$, $-10^{\circ}C$, and $-15^{\circ}C$. Then, the variation in the strength value was observed, depending on the freezing environment. In addition, the tests divided the loading rate into 6 phases and observed the variation in the stress-strain ratio, depending on the loading rate. The test data showed that a lower freezing temperature resulted in a larger strength value. An increase in the ice content in the specimen with the increase in the water content ratio influenced the strength value of the specimen. A faster load rate had a greater influence on the uniaxial compression and indirect tensile strengths of a frozen specimen and produced a different strength engineering property through the initial tangential modulus of elasticity. Finally, the long-term strength under a constant water content ratio and freezing temperature was checked by producing stress-strain ratio curves depending on the loading rate.

Potential Evaluation and Health Fostering Intrinsic Traits of Novel Probiotic Strain Enterococcus durans F3 Isolated from the Gut of Fresh Water Fish Catla catla

  • Alshammari, Eyad;Patel, Mitesh;Sachidanandan, Manojkumar;Kumar, Prashant;Adnan, Mohd
    • Food Science of Animal Resources
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    • v.39 no.5
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    • pp.844-861
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    • 2019
  • Over the last few years, marine environment was found to be a source of surplus natural products and microorganisms with new bioactive secondary metabolites of interest which can divulge nutritional and biological impact on the host. This study aims to assess the possible, inherent and functional probiotic properties of a novel probiotic strain Enterococcus durans F3 (E. durans F3) isolated from the gut of fresh water fish Catla catla. Parameters for evaluating and describing the probiotics described in FAD/WHO guidelines were followed. E. durans F3 demonstrated affirmative results including simulated bile, acid and gastric juice tolerance with exhibited significant bactericidal effect against pathogens Staphylococcus aureus, Salmonella Typhi, Escherichia coli and Pseudomonas aeruginosa. This can be due to the enterocin produced by E. durans F3 strain, which was resolute by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) gel with amplification of the anticipated fragment of a structural gene; enterocin A, followed by antibiotic susceptibility assessment. Effective antioxidant potentiality against ${\alpha}$-diphenyl-${\alpha}$-picrylhydrazyl free radicals including lipase, bile salt hydrolase activity with auto-aggregation and cell surface hydrophobicity was similarly observed. Results are proving the potentiality of E. durans F3, which can also be used as probiotic starter culture in dairy industries for manufacturing new products that imparts health benefits to the host. Finding the potent and novel probiotic strains will also satisfy the current developing market demand for probiotics.

A Study on Improvement of Correction Bracket Performance Using Optimal Structural Design (최적구조설계를 이용한 교정브라켓 성능향상에 관한 연구)

  • Li, Jung Hua;Kweon, Hyun Kyu;Gwon, Dong Jae;Park, Sang Jun;Jeon, Yoo Jin
    • Journal of the Semiconductor & Display Technology
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    • v.21 no.1
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    • pp.27-32
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    • 2022
  • There has always been a demand for orthodontic treatment. Orthodontic treatment allows tooth to be arranged by flexible arch wire fixed with tooth-attached brackets. Arch wire generate constant pressure to tooth brackets which moves the teeth to proper place. When the bracket transmits force, the braced wing of the bracket may deform. Deformed tie wing will lead to lost tension of elastic ligature. Then, lacking grip between tie wing and ligature might delay the tooth movement. Furthermore, tooth brackets used for orthodontic treatment make contact with in direct oral surface and this cause feeling of irritation that comes from height of tooth braces. This study suggests an optimal teethe bracket design to make up for inconvenience by shorten the height of bracket and complement the shape of bracket to reduce strain rate using finite element analysis. As a result, new optimal design of teethe bracket indicates lower strain rate of the bracket wing and takes good effects of shorten body height in terms of convenience.

A Study on Hull Deflection and Shaft Alignment Interaction in VLCC

  • Lee Yong-Jin;Kim Ue-Kan
    • Journal of Advanced Marine Engineering and Technology
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    • v.29 no.7
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    • pp.785-794
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    • 2005
  • Modern ship hulls of large oil carriers and container carrers have become more flexible with scantling optimization and increase in ship length. On the other hand. as the demand for power has increased with the ship size. shaft diameters have become larger and stiffer. Consequently. the alignment of the propulsion system has become more sensitive to hull girder deflections. resulting in difficulties in analyzing the alignment and conducting the alignment procedure. Accordingly. the frequency of shaft alignment related bearing damages has increased significantly in recent years. The alignment related damages are mostly attributed to inadequate analyses. changes in the design of the vessel. shipyards' practices in conducting the alignment. and a lack of well defined analytical criteria. The hull deflections should be considered at the design stage to minimize the bearing damage caused by hull deflection. Hull deflections can be estimated by analytical approach and reverse calculation using the measured data. The hull girder deflection analysis using the reverse calculation will be introduced in this paper.

Die and mold technology of in-mold labeling in functional packaging (기능성 생활용기 인 몰드 라벨링 금형 성형 기술)

  • Kim, Yu-Jin;Jung, Gui-Jae;Gong, Sung-Ho;Shin, Jang-Soon;Yoon, Gil-Sang;Jung, Woo-Chul;Kim, Gun-Hee;Seo, Tae-Il
    • Design & Manufacturing
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    • v.2 no.6
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    • pp.17-22
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    • 2008
  • Recently, the demand of high-productivity injection mold increases since the consumption of packaging grows in the world. Stack mold is composed of more than two molds and it has very high productivity and economic efficiency. In advanced country, stack mold which has was developed already but, in occasion of domestic mold industry, there is no study of stack mold. In this study, die and mold of in-mold labeling was developed for securing the technique of manufacturing high-productivity mold.

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