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http://dx.doi.org/10.5851/kosfa.2019.e57

Potential Evaluation and Health Fostering Intrinsic Traits of Novel Probiotic Strain Enterococcus durans F3 Isolated from the Gut of Fresh Water Fish Catla catla  

Alshammari, Eyad (Department of Clinical Nutrition, College of Applied Medical Sciences, University of Hail)
Patel, Mitesh (Department of Biosciences, Bapalal Vaidya Botanical Research Centre, Veer Narmad South Gujarat University)
Sachidanandan, Manojkumar (Department of Oral Radiology, College of Dentistry, University of Hail)
Kumar, Prashant (Ingress Bioinnovation)
Adnan, Mohd (Department of Biology, College of Science, University of Hail)
Publication Information
Food Science of Animal Resources / v.39, no.5, 2019 , pp. 844-861 More about this Journal
Abstract
Over the last few years, marine environment was found to be a source of surplus natural products and microorganisms with new bioactive secondary metabolites of interest which can divulge nutritional and biological impact on the host. This study aims to assess the possible, inherent and functional probiotic properties of a novel probiotic strain Enterococcus durans F3 (E. durans F3) isolated from the gut of fresh water fish Catla catla. Parameters for evaluating and describing the probiotics described in FAD/WHO guidelines were followed. E. durans F3 demonstrated affirmative results including simulated bile, acid and gastric juice tolerance with exhibited significant bactericidal effect against pathogens Staphylococcus aureus, Salmonella Typhi, Escherichia coli and Pseudomonas aeruginosa. This can be due to the enterocin produced by E. durans F3 strain, which was resolute by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) gel with amplification of the anticipated fragment of a structural gene; enterocin A, followed by antibiotic susceptibility assessment. Effective antioxidant potentiality against ${\alpha}$-diphenyl-${\alpha}$-picrylhydrazyl free radicals including lipase, bile salt hydrolase activity with auto-aggregation and cell surface hydrophobicity was similarly observed. Results are proving the potentiality of E. durans F3, which can also be used as probiotic starter culture in dairy industries for manufacturing new products that imparts health benefits to the host. Finding the potent and novel probiotic strains will also satisfy the current developing market demand for probiotics.
Keywords
Enterococcus; Catla catla; probiotics; enterocin; intestinal microbiota;
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